(Circular) (Six-base) MAPSORT of: pFN21AE6235.seq Check: 2716 from: 1 to: 5452 pFN21AE6235 5452 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3262 278 Size: 53 83 186 936 1726 2468 Fragments arranged by size: 2468 1726 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2795 1937 Size: 858 4594 Acc65I G'GTAC_C Cuts at: 2773 2773 Size: 5452 AclI AA'CG_TT Cuts at: 1563 3579 3952 1563 Size: 2016 373 3063 Fragments arranged by size: 3063 2016 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3515 4899 5167 1632 Size: 1883 1384 268 1917 Fragments arranged by size: 1917 1883 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5255 AflIII A'CryG_T Cuts at: 1203 2243 4351 5328 1203 Size: 1040 2108 977 1327 Fragments arranged by size: 2108 1327 1040 977 AgeI A'CCGG_T Cuts at: 4283 4283 Size: 5452 AhdI GACnn_n'nnGTC Cuts at: 4181 4181 Size: 5452 AleI CACnn'nnGTG Cuts at: 1166 2093 1166 Size: 927 4525 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5452 AlwNI CAG_nnn'CTG Cuts at: 2526 3195 4767 2526 Size: 669 1572 3211 Fragments arranged by size: 3211 1572 669 ApaLI G'TGCA_C Cuts at: 3509 4665 3509 Size: 1156 4296 ApoI r'AATT_y Cuts at: 1493 2761 2987 3139 5248 1493 Size: 1268 226 152 2109 1697 Fragments arranged by size: 2109 1697 1268 226 152 AseI AT'TA_AT Cuts at: 160 1032 4006 160 Size: 872 2974 1606 Fragments arranged by size: 2974 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5452 AvaI C'yCGr_G Cuts at: 1870 1943 2777 1870 Size: 73 834 4545 Fragments arranged by size: 4545 834 73 BamHI G'GATC_C Cuts at: 2782 3149 2782 Size: 367 5085 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2138 2201 2773 4228 618 Size: 325 217 627 351 63 572 1455 1842 Fragments arranged by size: 1842 1455 627 572 351 325 217 63 BanII G_rGCy'C Cuts at: 1391 2313 2771 1391 Size: 922 458 4072 Fragments arranged by size: 4072 922 458 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2071 928 Size: 971 172 4309 Fragments arranged by size: 4309 971 172 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3643 3677 1903 Size: 34 1706 34 3678 Fragments arranged by size: 3678 1706 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3344 4554 3344 Size: 1210 4242 BclI T'GATC_A Cuts at: 1548 1548 Size: 5452 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4063 136 Size: 107 122 71 631 605 2391 1525 Fragments arranged by size: 2391 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5447 5447 Size: 5452 BlpI GC'TnA_GC Cuts at: 2307 2869 2307 Size: 562 4890 Bme1580I G_kGCm'C Cuts at: 1790 2141 3513 4669 1790 Size: 351 1372 1156 2573 Fragments arranged by size: 2573 1372 1156 351 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5452 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2100 2677 3183 Size: 416 53 83 399 24 683 577 506 Cuts at: 3183 4141 442 Size: 958 1753 Fragments arranged by size: 1753 958 683 577 506 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5452 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2500 2522 2676 4112 1239 Size: 1261 22 154 1436 2579 Fragments arranged by size: 2579 1436 1261 154 22 Bpu10I CC'TnA_GC Cuts at: 2447 2447 Size: 5452 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3577 4442 4740 3577 Size: 865 298 4289 Fragments arranged by size: 4289 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2018 4115 882 Size: 617 519 2097 2219 Fragments arranged by size: 2219 2097 617 519 BsaAI yAC'GTr Cuts at: 493 1204 3243 493 Size: 711 2039 2702 Fragments arranged by size: 2702 2039 711 BsaBI GATnn'nnATC Cuts at: 2547 5446 2547 Size: 2899 2553 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3259 3641 4276 275 Size: 53 83 186 936 1726 382 635 1451 Fragments arranged by size: 1726 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3885 4283 4557 4704 5115 3885 Size: 398 274 147 411 4222 Fragments arranged by size: 4222 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2161 2191 1178 Size: 30 165 30 758 30 4439 Fragments arranged by size: 4439 758 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2029 2048 2428 1186 Size: 843 19 380 4210 Fragments arranged by size: 4210 843 380 19 BseYI C'CCAG_C Cuts at: 2519 4655 5151 2519 Size: 2136 496 2820 Fragments arranged by size: 2820 2136 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2456 2483 1163 Size: 573 720 27 4132 Fragments arranged by size: 4132 720 573 27 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2902 3663 4691 1540 Size: 275 186 901 761 1028 2301 Fragments arranged by size: 2301 1028 901 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2190 2313 2486 3513 3598 4669 2190 Size: 123 173 1027 85 1071 2973 Fragments arranged by size: 2973 1071 1027 173 123 85 BsmI GAATG_Cn' Cuts at: 2675 2963 3056 2675 Size: 288 93 5071 Fragments arranged by size: 5071 288 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2141 2190 2313 2486 2771 3513 3598 Size: 399 351 49 123 173 285 742 85 Cuts at: 3598 4669 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 351 285 173 123 85 49 BspEI T'CCGG_A Cuts at: 5115 5115 Size: 5452 BspHI T'CATG_A Cuts at: 2344 3341 5071 2344 Size: 997 1730 2725 Fragments arranged by size: 2725 1730 997 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2807 844 Size: 1963 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3339 1394 Size: 429 1516 3507 Fragments arranged by size: 3507 1516 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2410 3947 4121 65 Size: 1183 1162 1537 174 1396 Fragments arranged by size: 1537 1396 1183 1162 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4096 4283 1700 Size: 110 2286 187 2869 Fragments arranged by size: 2869 2286 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5452 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5452 BssSI C'ACGA_G Cuts at: 3512 4524 3512 Size: 1012 4440 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5452 BstBI TT'CG_AA Cuts at: 4256 4256 Size: 5452 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5452 BstXI CCAn_nnnn'nTGG Cuts at: 2690 2690 Size: 5452 BstYI r'GATC_y Cuts at: 1972 2557 2782 3149 3534 3551 5003 5089 5101 Size: 585 225 367 385 17 1452 86 12 Cuts at: 5101 5447 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 585 385 367 346 225 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2366 2366 Size: 5452 BtgI C'CryG_G Cuts at: 513 1065 2004 2258 513 Size: 552 939 254 3707 Fragments arranged by size: 3707 939 552 254 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2635 487 Size: 683 56 1409 3304 Fragments arranged by size: 3304 1409 683 56 BtsI GCAGTG_nn' Cuts at: 2430 2968 3762 3782 2430 Size: 538 794 20 4100 Fragments arranged by size: 4100 794 538 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5452 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2194 2229 529 Size: 35 1630 35 3752 Fragments arranged by size: 3752 1630 35 35 DraI TTT'AAA Cuts at: 2756 2756 Size: 5452 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4459 809 Size: 507 3143 1802 Fragments arranged by size: 3143 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2577 2730 2899 Size: 54 1425 50 275 18 747 153 169 Cuts at: 2899 3788 5417 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 747 275 169 153 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2899 1537 Size: 275 1087 4090 Fragments arranged by size: 4090 1087 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3382 1353 Size: 2029 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4039 4413 4559 387 Size: 1064 138 2450 374 146 1280 Fragments arranged by size: 2450 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2311 2311 Size: 5452 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2500 2522 2676 3515 4112 4899 5167 Size: 393 868 22 154 839 597 787 268 Cuts at: 5167 1239 Size: 1524 Fragments arranged by size: 1524 868 839 787 597 393 268 154 22 EcoNI CCTnn'n_nnAGG Cuts at: 2365 2365 Size: 5452 EcoRI G'AATT_C Cuts at: 2761 3139 2761 Size: 378 5074 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2377 2377 Size: 5452 FspI TGC'GCA Cuts at: 3958 3958 Size: 5452 HaeII r_GCGC'y Cuts at: 1678 4599 1678 Size: 2921 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2029 2061 4093 4125 4167 Size: 32 135 32 457 32 2032 32 42 Cuts at: 4167 4199 1373 Size: 32 2626 Fragments arranged by size: 2626 2032 457 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2593 2796 3042 669 Size: 988 281 655 203 246 3079 Fragments arranged by size: 3079 988 655 281 246 203 HindIII A'AGCT_T Cuts at: 748 748 Size: 5452 HpaI GTT'AAC Cuts at: 2593 3042 2593 Size: 449 5003 KpnI G_GTAC'C Cuts at: 2777 2777 Size: 5452 MfeI C'AATT_G Cuts at: 3051 3051 Size: 5452 MluI A'CGCG_T Cuts at: 5328 5328 Size: 5452 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2092 4566 4750 1305 Size: 787 2474 184 2007 Fragments arranged by size: 2474 2007 787 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3630 Fragments arranged by size: 3630 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2093 3410 3769 3928 518 Size: 648 927 1317 359 159 2042 Fragments arranged by size: 2042 1317 927 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2523 2868 3545 4693 4938 1606 Size: 917 345 677 1148 245 2120 Fragments arranged by size: 2120 1148 917 677 345 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2258 513 Size: 552 939 254 3707 Fragments arranged by size: 3707 939 552 254 NdeI CA'TA_TG Cuts at: 387 387 Size: 5452 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5452 NotI GC'GGCC_GC Cuts at: 2899 2899 Size: 5452 NspI r_CATG'y Cuts at: 1132 1229 2247 2698 2810 4355 1132 Size: 97 1018 451 112 1545 2229 Fragments arranged by size: 2229 1545 1018 451 112 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5452 PciI A'CATG_T Cuts at: 2243 4351 2243 Size: 2108 3344 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5452 PfoI T'CCnGG_A Cuts at: 2499 2499 Size: 5452 PmeI GTTT'AAAC Cuts at: 2756 2756 Size: 5452 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3521 3553 5034 5066 3521 Size: 32 1481 32 3907 Fragments arranged by size: 3907 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5452 PsiI TTA'TAA Cuts at: 3022 3022 Size: 5452 PstI C_TGCA'G Cuts at: 830 2804 830 Size: 1974 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5452 PvuII CAG'CTG Cuts at: 2523 2523 Size: 5452 SacI G_AGCT'C Cuts at: 2313 2313 Size: 5452 SalI G'TCGA_C Cuts at: 1936 2794 1936 Size: 858 4594 SbfI CC_TGCA'GG Cuts at: 2804 2804 Size: 5452 ScaI AGT'ACT Cuts at: 3700 5412 3700 Size: 1712 3740 SfcI C'TryA_G Cuts at: 826 1045 2800 3935 4616 4807 826 Size: 219 1755 1135 681 191 1471 Fragments arranged by size: 1755 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5452 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5452 SmaI CCC'GGG Cuts at: 1872 2779 1872 Size: 907 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3556 4457 4719 820 Size: 197 926 1613 901 262 1553 Fragments arranged by size: 1613 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3243 493 Size: 2750 2702 SpeI A'CTAG_T Cuts at: 152 2621 152 Size: 2469 2983 SphI G_CATG'C Cuts at: 1132 2698 2810 1132 Size: 1566 112 3774 Fragments arranged by size: 3774 1566 112 SspI AAT'ATT Cuts at: 5 52 3376 5 Size: 47 3324 2081 Fragments arranged by size: 3324 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5452 StyI C'CwwG_G Cuts at: 513 1065 2004 2258 2891 513 Size: 552 939 254 633 3074 Fragments arranged by size: 3074 939 633 552 254 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3479 3496 3649 3834 4279 325 Size: 1255 1899 17 153 185 445 1498 Fragments arranged by size: 1899 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3698 5410 96 Size: 274 80 33 51 1443 1721 1712 138 Fragments arranged by size: 1721 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2161 2193 5412 5444 2161 Size: 32 3219 32 2169 Fragments arranged by size: 3219 2169 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5452 XbaI T'CTAG_A Cuts at: 2788 2788 Size: 5452 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5452 XmaI C'CCGG_G Cuts at: 1870 2777 1870 Size: 907 4545 XmnI GAAnn'nnTTC Cuts at: 3581 3581 Size: 5452 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3260 276 Size: 53 83 186 936 1726 2468 Fragments arranged by size: 2468 1726 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI