Restriction map for
pFN21AE2404
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BbsI BcgI BciVI BclI
BfaI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MflI MluI MroI
MslI MunI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
RcaI RsaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AflII 334 CTTAAG C^TTAAG
AlwI 480 GATCC GGATC(4/5)
AlwNI 145 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 670 ATTAAT AT^TAAT
AsnI 670 ATTAAT AT^TAAT
AspHI 63 GAGCTC G[AT]GC[AT]^C
BalI 579 TGGCCA TGG^CCA
BanI 867 GGTGCC G^G[CT][GA]CC
BanII 63 GAGCTC G[GA]GC[CT]^C
BfrI 334 CTTAAG C^TTAAG
BmyI 63 GAGCTC G[AGT]GC[ACT]^C
BsaI 172 GAGACC GGTCTC(1/5)
BsiHKAI 63 GAGCTC G[AT]GC[AT]^C
BsmAI 172 GAGAC GTCTC(1/5)
Bsp1286I 63 GAGCTC G[AGT]GC[ACT]^C
BspMI 15 ACCTGC ACCTGC(4/8)
BsrDI 467 GCAATG GCAATG(2/0)
BstXI 498 CCAGAATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 550 GACGAGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 576 CCGTGG C^C[GA][CT]GG
EcoT14I 582 CCAAGG C^C[AT][AT]GG
FauI 87 CCCGC CCCGC(4/6)
HaeII 842 AGCGCC [GA]GCGC^[CT]
HgaI 221 GCGTC GACGC(5/10)
HincII 223 GTCAAC GT[CT]^[GA]AC
HindII 223 GTCAAC GT[CT]^[GA]AC
MaeII 683 ACGT A^CGT
MaeIII 539 GTGAC ^GT[ACGT]AC
MluNI 579 TGGCCA TGG^CCA
MscI 579 TGGCCA TGG^CCA
NdeI 1050 CATATG CA^TATG
PshBI 670 ATTAAT AT^TAAT
PvuII 148 CAGCTG CAG^CTG
SacI 63 GAGCTC GAGCT^C
SfcI 936 CTGTAG C^T[GA][CT]AG
StyI 582 CCAAGG C^C[AT][AT]GG
Tsp45I 539 GTGAC ^GT[GC]AC
XcmI 962 CCAAGTCCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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Asp700I 604 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
735 GAAAGTGTTC
BbvCI 102 GCTGAGG CCTCAGC(-5/-2)
618 GCTGAGG
BcnI 393 CCGGG CC^[GC]GG
483 CCGGG
BsiYI 477 CCTGATCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
903 CCTTATTCTGG
BslI 477 CCTGATCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
903 CCTTATTCTGG
BsmFI 945 GGGAC GGGAC(10/14)
85 GTCCC
BsrI 243 CCAGT ACTGG(1/-1)
744 CCAGT
Cac8I 98 GCTGGC GC[ACGT]^[ACGT]GC
123 GCGAGC
CfoI 843 GCGC GCG^C
924 GCGC
DraII 191 AGGACCC [GA]G^G[ACGT]CC[CT]
953 AGGACCC
EaeI 573 TGGCCG [CT]^GGCC[GA]
579 TGGCCA
EarI 187 GAAGAG CTCTTC(1/4)
688 GAAGAG
EcoO109I 191 AGGACCC [GA]G^G[ACGT]CC[CT]
953 AGGACCC
HapII 393 CCGG C^CGG
483 CCGG
HhaI 843 GCGC GCG^C
924 GCGC
HinP1I 843 GCGC G^CGC
924 GCGC
HpaII 393 CCGG C^CGG
483 CCGG
Ksp632I 187 GAAGAG CTCTTC(1/4)
688 GAAGAG
MspI 393 CCGG C^CGG
483 CCGG
NciI 393 CCGGG CC^[GC]GG
483 CCGGG
NlaIII 598 CATG CATG^
696 CATG
PleI 895 GACTC GAGTC(4/5)
644 GAGTC
PpuMI 191 AGGACCC [GA]G^G[AT]CC[CT]
953 AGGACCC
SfaNI 781 GATGC GCATC(5/9)
458 GCATC
TfiI 833 GATTC G^A[AT]TC
874 GAATC
XmnI 604 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
735 GAAAGTGTTC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BbvI BsaJI BseRI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI MboI MboII MnlI MseI
MspA1I MvaI MwoI NdeII NlaIV
Sau3AI Sau96I ScrFI SmlI Tru9I
TseI Tsp509I TspRI