################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvrII BalI BanI BbeI BbrPI BbvCI BcgI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI KpnI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SspBI SspI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AatII 1123 GACGTC GACGT^C AlwNI 1075 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 589 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 1239 TGCGCA TGC^GCA BamHI 1112 GGATCC G^GATCC BciVI 846 GGATAC GTATCC(6/5) BfaI 380 CTAG C^TAG BglI 1095 GCCTGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 78 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1066 TCCGGT [AT]^CCGG[AT] BsgI 226 GTGCAG GTGCAG(16/14) BsiEI 1155 CGACCG CG[GA][CT]^CG BsrBI 1214 GAGCGG CCGCTC(-3/-3) BstAPI 21 GCAACCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DsaI 1158 CCGCGG C^C[GA][CT]GG EaeI 1145 TGGCCG [CT]^GGCC[GA] EcoT14I 824 CCAAGG C^C[AT][AT]GG FspI 1239 TGCGCA TGC^GCA HaeII 1177 AGCGCC [GA]GCGC^[CT] KspI 1158 CCGCGG CCGC^GG MaeI 380 CTAG C^TAG MamI 78 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 1004 TTAA T^TAA NspI 897 GCATGC [GA]CATG^[CT] PstI 1097 CTGCAG CTGCA^G SacI 154 GAGCTC GAGCT^C SacII 1158 CCGCGG CCGC^GG SbfI 1096 CCTGCAGG CCTGCA^GG SphI 897 GCATGC GCATG^C Sse8387I 1096 CCTGCAGG CCTGCA^GG StyI 824 CCAAGG C^C[AT][AT]GG Tru9I 1004 TTAA T^TAA Tsp509I 243 AATT ^AATT XcmI 575 CCATTCTGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 589 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcyI 1048 GGCGTC G[GA]^CG[CT]C 1123 GACGTC AspHI 154 GAGCTC G[AT]GC[AT]^C 948 GTGCTC AvaI 1181 CCCGGG C^[CT]CG[GA]G 1255 CCCGGG BsaHI 1048 GGCGTC G[GA]^CG[CT]C 1123 GACGTC BsiHKAI 154 GAGCTC G[AT]GC[AT]^C 948 GTGCTC BsoBI 1181 CCCGGG C^[CT]CG[GA]G 1255 CCCGGG BssHII 1195 GCGCGC G^CGCGC 1197 GCGCGC BstXI 390 CCAGGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 543 CCATTTTGATGG EcoNI 447 CCTGAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 682 CCTGATGGAGG HgaI 110 GACGC GACGC(5/10) 1049 GCGTC Hin1I 1048 GGCGTC G[GA]^CG[CT]C 1123 GACGTC HphI 1120 GGTGA GGTGA(8/7) 740 TCACC MslI 544 CATTTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 741 CACCTTCGTG MspA1I 13 CCGCTG C[AC]G^C[GT]G 1158 CCGCGG PleI 813 GACTC GAGTC(4/5) 98 GAGTC SapI 676 GAAGAGC GCTCTTC(1/4) 1170 GAAGAGC SfcI 216 CTACAG C^T[GA][CT]AG 1097 CTGCAG SmaI 1181 CCCGGG CCC^GGG 1255 CCCGGG Tsp45I 754 GTCAC ^GT[GC]AC 1121 GTGAC XmaCI 1181 CCCGGG C^CCGGG 1255 CCCGGG XmaI 1181 CCCGGG C^CCGGG 1255 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AccII AciI AfaI AluI AlwI ApaI AvaII BanII BbsI BbvI BcnI BmyI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI TseI TspRI TthHB8I Van91I XhoII