Restriction map for
pFN21AE5504
(Circular) (Six-base) MAPSORT of: pFN21AE5504.seq Check: 5737 from: 1 to: 5965
pFN21AE5504 5965 bp
With 182 enzymes: SgfI *
December 8, 2014 13:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3132 3775 278
Size: 53 83 186 936 1596 643 2468
Fragments arranged by size:
2468 1596 936 643 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3308 1937
Size: 1371 4594
Acc65I G'GTAC_C
Cuts at: 3286 3286
Size: 5965
AclI AA'CG_TT
Cuts at: 1563 4092 4465 1563
Size: 2529 373 3063
Fragments arranged by size:
3063 2529 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2323 2700 4028 5412 5680 1632
Size: 691 377 1328 1384 268 1917
Fragments arranged by size:
1917 1384 1328 691 377 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5768
AflIII A'CryG_T
Cuts at: 1203 4864 5841 1203
Size: 3661 977 1327
Fragments arranged by size:
3661 1327 977
AgeI A'CCGG_T
Cuts at: 4796 4796
Size: 5965
AhdI GACnn_n'nnGTC
Cuts at: 4694 4694
Size: 5965
AleI CACnn'nnGTG
Cuts at: 1166 2750 1166
Size: 1584 4381
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5965
AlwNI CAG_nnn'CTG
Cuts at: 3085 3708 5280 3085
Size: 623 1572 3770
Fragments arranged by size:
3770 1572 623
ApaI G_GGCC'C
Cuts at: 2179 2810 2899 3111 2179
Size: 631 89 212 5033
Fragments arranged by size:
5033 631 212 89
ApaLI G'TGCA_C
Cuts at: 4022 5178 4022
Size: 1156 4809
ApoI r'AATT_y
Cuts at: 1493 3274 3500 3652 5761 1493
Size: 1781 226 152 2109 1697
Fragments arranged by size:
2109 1781 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4519 160
Size: 872 3487 1606
Fragments arranged by size:
3487 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5965
AvaI C'yCGr_G
Cuts at: 1870 1943 3186 3260 3290 1870
Size: 73 1243 74 30 4545
Fragments arranged by size:
4545 1243 74 73 30
BamHI G'GATC_C
Cuts at: 3117 3295 3662 3117
Size: 178 367 5420
Fragments arranged by size:
5420 367 178
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3286 4741 618
Size: 325 217 627 1499 1455 1842
Fragments arranged by size:
1842 1499 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2163 2179 2526 2717 2810 2899 3111 3284
Size: 772 16 347 191 93 89 212 173
Cuts at: 3284 1391
Size: 4072
Fragments arranged by size:
4072 772 347 212 191 173 93 89
16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2527 2644 3124 3148 928
Size: 971 628 117 480 24 3745
Fragments arranged by size:
3745 971 628 480 117 24
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4156 4190 1903
Size: 34 2219 34 3678
Fragments arranged by size:
3678 2219 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2845 3857 5067 2845
Size: 1012 1210 3743
Fragments arranged by size:
3743 1210 1012
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5965
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3106 4576 136
Size: 107 122 71 631 605 1434 1470 1525
Fragments arranged by size:
1525 1470 1434 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5960 5960
Size: 5965
BlpI GC'TnA_GC
Cuts at: 3382 3382
Size: 5965
Bme1580I G_kGCm'C
Cuts at: 1790 2179 2349 2810 2861 2899 3111 4026 5182
Size: 389 170 461 51 38 212 915 1156
Cuts at: 5182 1790
Size: 2573
Fragments arranged by size:
2573 1156 915 461 389 212 170 51
38
BmgBI CAC'GTC
Cuts at: 1318 2862 1318
Size: 1544 4421
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2442 3696 4654
Size: 416 53 83 399 24 1025 1254 958
Cuts at: 4654 442
Size: 1753
Fragments arranged by size:
1753 1254 1025 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5965
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3242 3242
Size: 5965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4625 1239
Size: 3386 2579
Bpu10I CC'TnA_GC
Cuts at: 2453 2453
Size: 5965
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4090 4955 5253 4090
Size: 865 298 4802
Fragments arranged by size:
4802 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4628 882
Size: 617 3129 2219
Fragments arranged by size:
3129 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3756 493
Size: 711 2552 2702
Fragments arranged by size:
2702 2552 711
BsaBI GATnn'nnATC
Cuts at: 2087 5959 2087
Size: 3872 2093
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3054 3129 3772 4154
Size: 53 83 186 936 1521 75 643 382
Cuts at: 4154 4789 275
Size: 635 1451
Fragments arranged by size:
1521 1451 936 643 635 382 186 83
75 53
BsaWI w'CCGG_w
Cuts at: 3071 4398 4796 5070 5217 5628 3071
Size: 1327 398 274 147 411 3408
Fragments arranged by size:
3408 1327 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2282 2312 2688 2718 1178
Size: 30 165 30 879 30 376 30 4425
Fragments arranged by size:
4425 879 376 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2290 2709 2900 1186
Size: 1104 419 191 4251
Fragments arranged by size:
4251 1104 419 191
BseYI C'CCAG_C
Cuts at: 2254 2525 2933 5168 5664 2254
Size: 271 408 2235 496 2555
Fragments arranged by size:
2555 2235 496 408 271
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2252 1163
Size: 573 516 4876
Fragments arranged by size:
4876 573 516
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3163 3415 4176 5204 1540
Size: 275 186 1162 252 761 1028 2301
Fragments arranged by size:
2301 1162 1028 761 275 252 186
BsiHKAI G_wGCw'C
Cuts at: 2163 2957 4026 4111 5182 2163
Size: 794 1069 85 1071 2946
Fragments arranged by size:
2946 1071 1069 794 85
BsmI GAATG_Cn'
Cuts at: 3476 3569 3476
Size: 93 5872
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2163 2179 2349 2526 2717 2810 2861
Size: 399 373 16 170 177 191 93 51
Cuts at: 2861 2899 2957 3111 3284 4026 4111 5182 1391
Size: 38 58 154 173 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 373 191 177 173
170 154 93 85 58 51 38 16
BspEI T'CCGG_A
Cuts at: 5628 5628
Size: 5965
BspHI T'CATG_A
Cuts at: 3854 5584 3854
Size: 1730 4235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3320 844
Size: 2476 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3221 3852 1394
Size: 429 1398 631 3507
Fragments arranged by size:
3507 1398 631 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4460 4634 65
Size: 1183 3212 174 1396
Fragments arranged by size:
3212 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4609 4796 1700
Size: 110 2799 187 2869
Fragments arranged by size:
2869 2799 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5965
BssHII G'CGCG_C
Cuts at: 1925 3200 3202 1925
Size: 1275 2 4688
Fragments arranged by size:
4688 1275 2
BssSI C'ACGA_G
Cuts at: 4025 5037 4025
Size: 1012 4953
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2032 794
Size: 1238 4727
BstBI TT'CG_AA
Cuts at: 4769 4769
Size: 5965
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5965
BstXI CCAn_nnnn'nTGG
Cuts at: 2402 2555 2402
Size: 153 5812
BstYI r'GATC_y
Cuts at: 1972 2781 2889 3117 3295 3662 4047 4064 5516
Size: 809 108 228 178 367 385 17 1452
Cuts at: 5516 5602 5614 5960 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 809 385 367 346 228 178
108 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3163 513
Size: 552 2098 3315
Fragments arranged by size:
3315 2098 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5226
Fragments arranged by size:
5226 683 56
BtsI GCAGTG_nn'
Cuts at: 3481 4275 4295 3481
Size: 794 20 5151
Fragments arranged by size:
5151 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5965
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5930
DraI TTT'AAA
Cuts at: 3269 3269
Size: 5965
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4972 809
Size: 507 3656 1802
Fragments arranged by size:
3656 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3150 3412 4301
Size: 54 1425 50 275 18 1320 262 889
Cuts at: 4301 5930 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1320 889 275 262 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3412 1537
Size: 275 1600 4090
Fragments arranged by size:
4090 1600 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2100 2676 3170 3895 1353
Size: 747 576 494 725 3423
Fragments arranged by size:
3423 747 725 576 494
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3047 3087 4552 4926 5072 387
Size: 1064 138 1458 40 1465 374 146 1280
Fragments arranged by size:
1465 1458 1280 1064 374 146 138 40
EcoICRI GAG'CTC
Cuts at: 2161 2161
Size: 5965
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2323 2700 4028 4625 5412 5680 1239
Size: 393 691 377 1328 597 787 268 1524
Fragments arranged by size:
1524 1328 787 691 597 393 377 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2456 2691 2456
Size: 235 5730
EcoO109I rG'GnC_Cy
Cuts at: 2121 2175 2176 2314 2674 2876 2896 3082 3107
Size: 54 1 138 360 202 20 186 25
Cuts at: 3107 3108 2121
Size: 1 4978
Fragments arranged by size:
4978 360 202 186 138 54 25 20
1 1
EcoRI G'AATT_C
Cuts at: 3274 3652 3274
Size: 378 5587
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2218 2668 2218
Size: 450 5515
FspI TGC'GCA
Cuts at: 3246 4471 3246
Size: 1225 4740
HaeII r_GCGC'y
Cuts at: 1678 3186 5112 1678
Size: 1508 1926 2531
Fragments arranged by size:
2531 1926 1508
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2282 2314 2688 2720 4606
Size: 32 135 32 710 32 374 32 1886
Cuts at: 4606 4638 4680 4712 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1886 710 374 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3309 3555 669
Size: 988 281 1371 246 3079
Fragments arranged by size:
3079 1371 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5965
HpaI GTT'AAC
Cuts at: 3555 3555
Size: 5965
KpnI G_GTAC'C
Cuts at: 3290 3290
Size: 5965
MfeI C'AATT_G
Cuts at: 3564 3564
Size: 5965
MluI A'CGCG_T
Cuts at: 5841 5841
Size: 5965
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2131 5079 5263 1305
Size: 826 2948 184 2007
Fragments arranged by size:
2948 2007 826 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4143
Fragments arranged by size:
4143 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2553 2750 3923 4282 4441 518
Size: 648 1387 197 1173 359 159 2042
Fragments arranged by size:
2042 1387 1173 648 359 197 159
MspA1I CmG'CkG
Cuts at: 1606 2020 3165 3381 4058 5206 5451 1606
Size: 414 1145 216 677 1148 245 2120
Fragments arranged by size:
2120 1148 1145 677 414 245 216
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5413
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5965
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5965
NotI GC'GGCC_GC
Cuts at: 3412 3412
Size: 5965
NspI r_CATG'y
Cuts at: 1132 1229 2906 3323 4868 1132
Size: 97 1677 417 1545 2229
Fragments arranged by size:
2229 1677 1545 417 97
PasI CC'CwG_GG
Cuts at: 1348 2186 2810 1348
Size: 838 624 4503
Fragments arranged by size:
4503 838 624
PciI A'CATG_T
Cuts at: 4864 4864
Size: 5965
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2186 2310 3085 1108
Size: 1078 124 775 3988
Fragments arranged by size:
3988 1078 775 124
PfoI T'CCnGG_A
Cuts at: 2321 2847 2321
Size: 526 5439
PmeI GTTT'AAAC
Cuts at: 3269 3269
Size: 5965
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4034 4066 5547 5579 4034
Size: 32 1481 32 4420
Fragments arranged by size:
4420 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2121 2314 2674 2876 2121
Size: 193 360 202 5210
Fragments arranged by size:
5210 360 202 193
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5965
PsiI TTA'TAA
Cuts at: 3535 3535
Size: 5965
PspOMI G'GGCC_C
Cuts at: 2175 2806 2895 3107 2175
Size: 631 89 212 5033
Fragments arranged by size:
5033 631 212 89
PstI C_TGCA'G
Cuts at: 830 3106 3317 830
Size: 2276 211 3478
Fragments arranged by size:
3478 2276 211
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5965
SacI G_AGCT'C
Cuts at: 2163 2163
Size: 5965
SacII CC_GC'GG
Cuts at: 3166 3166
Size: 5965
SalI G'TCGA_C
Cuts at: 1936 3307 1936
Size: 1371 4594
SanDI GG'GwC_CC
Cuts at: 2314 2674 2314
Size: 360 5605
SapI GCTCTTCn'nnn_
Cuts at: 2676 3170 2676
Size: 494 5471
SbfI CC_TGCA'GG
Cuts at: 3106 3317 3106
Size: 211 5754
ScaI AGT'ACT
Cuts at: 4213 5925 4213
Size: 1712 4253
SfcI C'TryA_G
Cuts at: 826 1045 2221 3102 3313 4448 5129 5320 826
Size: 219 1176 881 211 1135 681 191 1471
Fragments arranged by size:
1471 1176 1135 881 681 219 211 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5965
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5965
SmaI CCC'GGG
Cuts at: 1872 3188 3262 3292 1872
Size: 1316 74 30 4545
Fragments arranged by size:
4545 1316 74 30
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4069 4970 5232 820
Size: 197 926 2126 901 262 1553
Fragments arranged by size:
2126 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3756 493
Size: 3263 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5965
SphI G_CATG'C
Cuts at: 1132 2906 3323 1132
Size: 1774 417 3774
Fragments arranged by size:
3774 1774 417
SspI AAT'ATT
Cuts at: 5 52 3889 5
Size: 47 3837 2081
Fragments arranged by size:
3837 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5965
StyI C'CwwG_G
Cuts at: 513 1065 2829 3404 513
Size: 552 1764 575 3074
Fragments arranged by size:
3074 1764 575 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3992 4009 4162 4347 4792 325
Size: 1255 2412 17 153 185 445 1498
Fragments arranged by size:
2412 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2617 2940 4211
Size: 274 80 33 51 1443 640 323 1271
Cuts at: 4211 5923 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1271 640 323 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2718 2750 3109 3141 5925 5957 2718
Size: 32 359 32 2784 32 2726
Fragments arranged by size:
2784 2726 359 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5965
XbaI T'CTAG_A
Cuts at: 3301 3301
Size: 5965
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2587 2587
Size: 5965
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5965
XmaI C'CCGG_G
Cuts at: 1870 3186 3260 3290 1870
Size: 1316 74 30 4545
Fragments arranged by size:
4545 1316 74 30
XmnI GAAnn'nnTTC
Cuts at: 2598 4094 2598
Size: 1496 4469
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3130 3773 276
Size: 53 83 186 936 1596 643 2468
Fragments arranged by size:
2468 1596 936 643 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI PvuII RsrII SexAI SfiI SfoI
SrfI SwaI