(Linear) (Six-base) MAPSORT of: KIEE5504.seq Check: 9000 from: 1 to: 1260 KIEE5504.orf With 182 enzymes: SgfI * December 5, 2014 13:02 .. AatII G_ACGT'C Cuts at: 0 1127 1260 Size: 1127 133 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 318 695 1260 Size: 318 377 565 Fragments arranged by size: 565 377 318 AleI CACnn'nnGTG Cuts at: 0 745 1260 Size: 745 515 AlwNI CAG_nnn'CTG Cuts at: 0 1080 1260 Size: 1080 180 ApaI G_GGCC'C Cuts at: 0 174 805 894 1106 1260 Size: 174 631 89 212 154 Fragments arranged by size: 631 212 174 154 89 AvaI C'yCGr_G Cuts at: 0 1181 1255 1260 Size: 1181 74 5 Fragments arranged by size: 1181 74 5 BamHI G'GATC_C Cuts at: 0 1112 1260 Size: 1112 148 BanII G_rGCy'C Cuts at: 0 158 174 521 712 805 894 1106 1260 Size: 158 16 347 191 93 89 212 154 Fragments arranged by size: 347 212 191 158 154 93 89 16 BbsI GAAGACnn'nnnn_ Cuts at: 0 522 639 1119 1143 1260 Size: 522 117 480 24 117 Fragments arranged by size: 522 480 117 117 24 BciVI GTATCCnnnnn_n' Cuts at: 0 840 1260 Size: 840 420 BglI GCCn_nnn'nGGC Cuts at: 0 1101 1260 Size: 1101 159 Bme1580I G_kGCm'C Cuts at: 0 174 344 805 856 894 1106 1260 Size: 174 170 461 51 38 212 154 Fragments arranged by size: 461 212 174 170 154 51 38 BmgBI CAC'GTC Cuts at: 0 857 1260 Size: 857 403 BmrI ACTGGGnnnn_n' Cuts at: 0 437 1260 Size: 437 823 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1237 1260 Size: 1237 23 Bpu10I CC'TnA_GC Cuts at: 0 448 1260 Size: 448 812 BsaBI GATnn'nnATC Cuts at: 0 82 1260 Size: 82 1178 BsaHI Gr'CG_yC Cuts at: 0 1049 1124 1260 Size: 1049 75 136 Fragments arranged by size: 1049 136 75 BsaWI w'CCGG_w Cuts at: 0 1066 1260 Size: 1066 194 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 277 307 683 713 1260 Size: 277 30 376 30 547 Fragments arranged by size: 547 376 277 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 285 704 895 1260 Size: 285 419 191 365 Fragments arranged by size: 419 365 285 191 BseYI C'CCAG_C Cuts at: 0 249 520 928 1260 Size: 249 271 408 332 Fragments arranged by size: 408 332 271 249 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 247 1260 Size: 247 1013 BsiEI CG_ry'CG Cuts at: 0 1158 1260 Size: 1158 102 BsiHKAI G_wGCw'C Cuts at: 0 158 952 1260 Size: 158 794 308 Fragments arranged by size: 794 308 158 Bsp1286I G_dGCh'C Cuts at: 0 158 174 344 521 712 805 856 894 Size: 158 16 170 177 191 93 51 38 Cuts at: 894 952 1106 1260 Size: 58 154 154 Fragments arranged by size: 191 177 170 158 154 154 93 58 51 38 16 BsrBI CCG'CTC Cuts at: 0 1216 1260 Size: 1216 44 BssHII G'CGCG_C Cuts at: 0 1195 1197 1260 Size: 1195 2 63 Fragments arranged by size: 1195 63 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 27 1260 Size: 27 1233 BstXI CCAn_nnnn'nTGG Cuts at: 0 397 550 1260 Size: 397 153 710 Fragments arranged by size: 710 397 153 BstYI r'GATC_y Cuts at: 0 776 884 1112 1260 Size: 776 108 228 148 Fragments arranged by size: 776 228 148 108 BtgI C'CryG_G Cuts at: 0 1158 1260 Size: 1158 102 EaeI y'GGCC_r Cuts at: 0 1145 1260 Size: 1145 115 EarI CTCTTCn'nnn_ Cuts at: 0 95 671 1165 1260 Size: 95 576 494 95 Fragments arranged by size: 576 494 95 95 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1042 1082 1260 Size: 1042 40 178 Fragments arranged by size: 1042 178 40 EcoICRI GAG'CTC Cuts at: 0 156 1260 Size: 156 1104 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 318 695 1260 Size: 318 377 565 Fragments arranged by size: 565 377 318 EcoNI CCTnn'n_nnAGG Cuts at: 0 451 686 1260 Size: 451 235 574 Fragments arranged by size: 574 451 235 EcoO109I rG'GnC_Cy Cuts at: 0 116 170 171 309 669 871 891 1077 Size: 116 54 1 138 360 202 20 186 Cuts at: 1077 1102 1103 1260 Size: 25 1 157 Fragments arranged by size: 360 202 186 157 138 116 54 25 20 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 213 663 1260 Size: 213 450 597 Fragments arranged by size: 597 450 213 FspI TGC'GCA Cuts at: 0 1241 1260 Size: 1241 19 HaeII r_GCGC'y Cuts at: 0 1181 1260 Size: 1181 79 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 277 309 683 715 1260 Size: 277 32 374 32 545 Fragments arranged by size: 545 374 277 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 126 1260 Size: 126 1134 MslI CAynn'nnrTG Cuts at: 0 548 745 1260 Size: 548 197 515 Fragments arranged by size: 548 515 197 MspA1I CmG'CkG Cuts at: 0 15 1160 1260 Size: 15 1145 100 Fragments arranged by size: 1145 100 15 NspI r_CATG'y Cuts at: 0 901 1260 Size: 901 359 PasI CC'CwG_GG Cuts at: 0 181 805 1260 Size: 181 624 455 Fragments arranged by size: 624 455 181 PflMI CCAn_nnn'nTGG Cuts at: 0 181 305 1080 1260 Size: 181 124 775 180 Fragments arranged by size: 775 181 180 124 PfoI T'CCnGG_A Cuts at: 0 316 842 1260 Size: 316 526 418 Fragments arranged by size: 526 418 316 PpuMI rG'GwC_Cy Cuts at: 0 116 309 669 871 1260 Size: 116 193 360 202 389 Fragments arranged by size: 389 360 202 193 116 PspOMI G'GGCC_C Cuts at: 0 170 801 890 1102 1260 Size: 170 631 89 212 158 Fragments arranged by size: 631 212 170 158 89 PstI C_TGCA'G Cuts at: 0 1101 1260 Size: 1101 159 SacI G_AGCT'C Cuts at: 0 158 1260 Size: 158 1102 SacII CC_GC'GG Cuts at: 0 1161 1260 Size: 1161 99 SanDI GG'GwC_CC Cuts at: 0 309 669 1260 Size: 309 360 591 Fragments arranged by size: 591 360 309 SapI GCTCTTCn'nnn_ Cuts at: 0 671 1165 1260 Size: 671 494 95 Fragments arranged by size: 671 494 95 SbfI CC_TGCA'GG Cuts at: 0 1101 1260 Size: 1101 159 SfcI C'TryA_G Cuts at: 0 216 1097 1260 Size: 216 881 163 Fragments arranged by size: 881 216 163 SmaI CCC'GGG Cuts at: 0 1183 1257 1260 Size: 1183 74 3 Fragments arranged by size: 1183 74 3 SphI G_CATG'C Cuts at: 0 901 1260 Size: 901 359 StyI C'CwwG_G Cuts at: 0 824 1260 Size: 824 436 TatI w'GTAC_w Cuts at: 0 612 935 1260 Size: 612 323 325 Fragments arranged by size: 612 325 323 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 713 745 1104 1136 1260 Size: 713 32 359 32 124 Fragments arranged by size: 713 359 124 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 582 1260 Size: 582 678 XmaI C'CCGG_G Cuts at: 0 1181 1255 1260 Size: 1181 74 5 Fragments arranged by size: 1181 74 5 XmnI GAAnn'nnTTC Cuts at: 0 593 1260 Size: 593 667 ZraI GAC'GTC Cuts at: 0 1125 1260 Size: 1125 135 Enzymes that do cut: AatII AcuI AleI AlwNI ApaI AvaI BamHI BanII BbsI BciVI BglI Bme1580I BmgBI BmrI BplI Bpu10I BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrBI BssHII BstAPI BstXI BstYI BtgI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I MmeI MslI MspA1I NspI PasI PflMI PfoI PpuMI PspOMI PstI SacI SacII SanDI SapI SbfI SfcI SmaI SphI StyI TatI TstI XcmI XmaI XmnI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BanI BbeI BbvCI BcgI BclI BfrBI BglII BlpI BmtI BpmI BpuEI BsaI BsaAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssSI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV FseI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SalI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI TaqII Tth111I XbaI XhoI