Restriction map for
pFN21AE1334
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AlwI AlwNI AocI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BcgI BciVI
BclI BcnI BglI BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DrdI EaeI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO109I EcoO65I EcoRV EcoT22I FauI
FbaI FseI FspI HapII HgaI
Hin1I HincII HindII HpaI HpaII
HphI KasI KpnI KspI MaeIII
MamI MfeI MluI MluNI MroI
MscI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NlaIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI TaqI Tsp45I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcsI 337 GAATTC [GA]^AATT[CT]
AflII 31 CTTAAG C^TTAAG
AflIII 929 ACGTGT A^C[GA][CT]GT
Aor51HI 558 AGCGCT AGC^GCT
ApoI 337 GAATTC [GA]^AATT[CT]
AspHI 586 GAGCAC G[AT]GC[AT]^C
AvaII 283 GGACC G^G[AT]CC
BanII 954 GGGCTC G[GA]GC[CT]^C
BbrPI 883 CACGTG CAC^GTG
BbsI 151 GAAGAC GAAGAC(2/6)
BbvCI 561 GCTGAGG CCTCAGC(-5/-2)
BfaI 1015 CTAG C^TAG
BfrI 31 CTTAAG C^TTAAG
BglII 371 AGATCT A^GATCT
BpuAI 151 GAAGAC GAAGAC(2/6)
BsaAI 883 CACGTG [CT]AC^GT[GA]
BsiHKAI 586 GAGCAC G[AT]GC[AT]^C
BspHI 330 TCATGA T^CATGA
BsrDI 900 CATTGC GCAATG(2/0)
BstXI 542 CCAGGGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 371 AGATCT [GA]^GATC[CT]
Cac8I 769 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 559 GCGC GCG^C
DpnII 372 GATC ^GATC
DraIII 925 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 786 CCGTGG C^C[GA][CT]GG
Eco47III 558 AGCGCT AGC^GCT
EcoNI 289 CCTGAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 337 GAATTC G^AATTC
HaeII 558 AGCGCT [GA]GCGC^[CT]
HhaI 559 GCGC GCG^C
HinP1I 559 GCGC G^CGC
HindIII 319 AAGCTT A^AGCTT
MaeI 1015 CTAG C^TAG
MboI 372 GATC ^GATC
MflI 371 AGATCT [GA]^GATC[CT]
MslI 477 CACAGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 420 CATATG CA^TATG
NdeII 372 GATC ^GATC
NspI 982 ACATGC [GA]CATG^[CT]
PmaCI 883 CACGTG CAC^GTG
PmlI 883 CACGTG CAC^GTG
PstI 771 CTGCAG CTGCA^G
RcaI 330 TCATGA T^CATGA
SapI 911 GCTCTTC GCTCTTC(1/4)
Sau3AI 372 GATC ^GATC
StuI 767 AGGCCT AGG^CCT
Tsp509I 338 AATT ^AATT
TspRI 66 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 371 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
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Asp700I 891 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1021 GAAGTATTTC
BbvI 684 GCTGC GCAGC(8/12)
1007 GCTGC
BmyI 586 GAGCAC G[AGT]GC[ACT]^C
954 GGGCTC
Bsp1286I 586 GAGCAC G[AGT]GC[ACT]^C
954 GGGCTC
BsrI 67 ACTGG ACTGG(1/-1)
107 ACTGG
Cfr13I 283 GGACC G^G[ACGT]CC
1061 GGGCC
Fnu4HI 684 GCTGC GC^[ACGT]GC
1007 GCTGC
ItaI 684 GCTGC GC^[ACGT]GC
1007 GCTGC
MseI 32 TTAA T^TAA
230 TTAA
Sau96I 283 GGACC G^G[ACGT]CC
1061 GGGCC
SspI 131 AATATT AAT^ATT
995 AATATT
Tru9I 32 TTAA T^TAA
230 TTAA
TseI 684 GCTGC G^C[AT]GC
1007 GCTGC
XmnI 891 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1021 GAAGTATTTC
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AfaI AluI BpmI BsaJI
BsiYI BslI BsmAI BssKI BstF5I
BstNI DdeI EarI EcoRII EcoT14I
FokI HaeIII HinfI Ksp632I MaeII
MboII MnlI MvaI MwoI NlaIII
PleI RsaI ScrFI SfaNI SfcI
SmlI StyI TfiI