################################## # NO CUT ENZYMES ( 190 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BcnI BglI BglII BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeIII MamI MfeI MflI MluI MroI MslI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfaNI SfcI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AfaI 295 GTAC GT^AC AflII 70 CTTAAG C^TTAAG AluI 209 AGCT AG^CT AlwI 150 GGATC GGATC(4/5) AocI 361 CCTGAGG CC^T[ACGT]AGG AvrII 225 CCTAGG C^CTAGG BbvCI 217 GCTGAGG CCTCAGC(-5/-2) BbvI 57 GCAGC GCAGC(8/12) BfaI 226 CTAG C^TAG BfrI 70 CTTAAG C^TTAAG BlnI 225 CCTAGG C^CTAGG BseRI 364 GAGGAG GAGGAG(10/8) BsiYI 283 CCTTGTATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 283 CCTTGTATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 329 GAATGC GAATGC(1/-1) BsrI 297 ACTGG ACTGG(1/-1) BssKI 42 CCAGG ^CC[ACGT]GG BstBI 449 TTCGAA TT^CGAA BstNI 42 CCAGG CC^[AT]GG Bsu36I 361 CCTGAGG CC^T[ACGT]AGG Cac8I 437 GCTGGC GC[ACGT]^[ACGT]GC Eco81I 361 CCTGAGG CC^T[ACGT]AGG EcoRII 42 CCAGG ^CC[AT]GG EcoT14I 225 CCTAGG C^C[AT][AT]GG Fnu4HI 57 GCAGC GC^[ACGT]GC ItaI 57 GCAGC GC^[ACGT]GC MaeI 226 CTAG C^TAG MaeII 342 ACGT A^CGT MvaI 42 CCAGG CC^[AT]GG NspV 449 TTCGAA TT^CGAA PleI 33 GAGTC GAGTC(4/5) RsaI 295 GTAC GT^AC ScaI 294 AGTACT AGT^ACT ScrFI 42 CCAGG CC^[ACGT]GG SfuI 449 TTCGAA TT^CGAA SmlI 70 CTTAAG C^T[CT][GA]AG SspI 111 AATATT AAT^ATT StyI 225 CCTAGG C^C[AT][AT]GG TaqI 450 TCGA T^CGA TfiI 230 GAATC G^A[AT]TC TseI 57 GCAGC G^C[AT]GC TthHB8I 450 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AvaII 45 GGACC G^G[AT]CC 222 GGTCC BalI 251 TGGCCA TGG^CCA 351 TGGCCA BpmI 200 CTCCAG CTGGAG(16/14) 298 CTGGAG BsaJI 41 CCCAGG C^C[ACGT][ACGT]GG 225 CCTAGG DpnII 151 GATC ^GATC 462 GATC DraII 44 AGGACCC [GA]G^G[ACGT]CC[CT] 221 AGGTCCT EaeI 251 TGGCCA [CT]^GGCC[GA] 351 TGGCCA EcoO109I 44 AGGACCC [GA]G^G[ACGT]CC[CT] 221 AGGTCCT HinfI 33 GAGTC G^A[ACGT]TC 230 GAATC MboI 151 GATC ^GATC 462 GATC MluNI 251 TGGCCA TGG^CCA 351 TGGCCA MscI 251 TGGCCA TGG^CCA 351 TGGCCA NdeII 151 GATC ^GATC 462 GATC PpuMI 44 AGGACCC [GA]G^G[AT]CC[CT] 221 AGGTCCT Sau3AI 151 GATC ^GATC 462 GATC Tsp509I 125 AATT ^AATT 310 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 10 enzymes) # ######################################### Cfr13I DdeI HaeIII MboII MnlI MseI NlaIV Sau96I Tru9I TspRI