(Linear) (Six-base) MAPSORT of: KIEE1337.seq Check: 5295 from: 1 to: 474 KIEE1337.orf With 182 enzymes: SgfI * July 15, 2010 18:36 .. AflII C'TTAA_G Cuts at: 0 70 474 Size: 70 404 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 298 330 474 Size: 298 32 144 Fragments arranged by size: 298 144 32 AvrII C'CTAG_G Cuts at: 0 225 474 Size: 225 249 BbvCI CC'TCA_GC Cuts at: 0 218 474 Size: 218 256 BmgBI CAC'GTC Cuts at: 0 343 474 Size: 343 131 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 185 319 474 Size: 185 134 155 Fragments arranged by size: 185 155 134 Bpu10I CC'TnA_GC Cuts at: 0 218 474 Size: 218 256 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 379 474 Size: 379 95 BsmI GAATG_Cn' Cuts at: 0 335 474 Size: 335 139 BstBI TT'CG_AA Cuts at: 0 450 474 Size: 450 24 Bsu36I CC'TnA_GG Cuts at: 0 362 474 Size: 362 112 BtsI GCAGTG_nn' Cuts at: 0 177 212 474 Size: 177 35 262 Fragments arranged by size: 262 177 35 EaeI y'GGCC_r Cuts at: 0 251 351 474 Size: 251 100 123 Fragments arranged by size: 251 123 100 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 185 319 474 Size: 185 134 155 Fragments arranged by size: 185 155 134 EcoO109I rG'GnC_Cy Cuts at: 0 45 222 474 Size: 45 177 252 Fragments arranged by size: 252 177 45 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 434 474 Size: 434 40 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 407 474 Size: 407 67 MscI TGG'CCA Cuts at: 0 253 353 474 Size: 253 100 121 Fragments arranged by size: 253 121 100 PpuMI rG'GwC_Cy Cuts at: 0 45 222 474 Size: 45 177 252 Fragments arranged by size: 252 177 45 ScaI AGT'ACT Cuts at: 0 296 474 Size: 296 178 SmlI C'TyrA_G Cuts at: 0 70 474 Size: 70 404 SspI AAT'ATT Cuts at: 0 113 474 Size: 113 361 StyI C'CwwG_G Cuts at: 0 225 474 Size: 225 249 TatI w'GTAC_w Cuts at: 0 294 474 Size: 294 180 Enzymes that do cut: AflII AloI AvrII BbvCI BmgBI BpmI Bpu10I BseRI BsmI BstBI Bsu36I BtsI EaeI Eco57MI EcoO109I FalI MmeI MscI PpuMI ScaI SmlI SspI StyI TatI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI