################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AviII BalI BamHI BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BglI BglII BpuAI BsaBI BsaHI BseAI BsgI BsiEI BsiWI BspDI BspEI BspLU11I BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MseI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspI SwaI TfiI Tru9I XbaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 477 GTCTAC GT^[AC][GT]AC AccII 1039 CGCG CG^CG AcsI 133 AAATTC [GA]^AATT[CT] AflIII 481 ACACGT A^C[GA][CT]GT Alw44I 582 GTGCAC G^TGCAC AlwNI 1353 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 582 GTGCAC G^TGCAC ApoI 133 AAATTC [GA]^AATT[CT] AspI 775 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1047 CCTAGG C^CTAGG BcgI 1011 GCACCAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 1048 CTAG C^TAG BlnI 1047 CCTAGG C^CTAGG BlpI 1161 GCTCAGC GC^T[ACGT]AGC BpmI 355 CTCCAG CTGGAG(16/14) Bpu1102I 1161 GCTCAGC GC^T[ACGT]AGC BsaAI 482 CACGTA [CT]AC^GT[GA] BsaI 1193 GGTCTC GGTCTC(1/5) BsaWI 839 ACCGGA [AT]^CCGG[AT] BsmBI 187 GAGACG CGTCTC(1/5) BsmI 1302 GAATGC GAATGC(1/-1) Bsp1407I 308 TGTACA T^GTACA BspHI 575 TCATGA T^CATGA BsrBI 790 GAGCGG CCGCTC(-3/-3) BsrGI 308 TGTACA T^GTACA BstUI 1039 CGCG CG^CG BstXI 357 CCAGTGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1161 GCTCAGC GC^T[ACGT]AGC DrdI 1264 GACCCTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 315 CCGTGG C^C[GA][CT]GG FauI 744 GCGGG CCCGC(4/6) MaeI 1048 CTAG C^TAG MvnI 1039 CGCG CG^CG NspI 168 ACATGC [GA]CATG^[CT] PaeR7I 175 CTCGAG C^TCGAG PflFI 775 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 149 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 320 GGGACCC [GA]G^G[AT]CC[CT] RcaI 575 TCATGA T^CATGA SexAI 776 ACCAGGT A^CC[AT]GGT SmaI 878 CCCGGG CCC^GGG SspBI 308 TGTACA T^GTACA StuI 372 AGGCCT AGG^CCT TaqI 176 TCGA T^CGA Tsp509I 134 AATT ^AATT Tth111I 775 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 176 TCGA T^CGA Van91I 149 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 153 CCACTGGCTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 175 CTCGAG C^TCGAG XmaCI 878 CCCGGG C^CCGGG XmaI 878 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AocI 325 CCTAAGG CC^T[ACGT]AGG 1267 CCTGAGG AspHI 582 GTGCAC G[AT]GC[AT]^C 1009 GAGCAC BsiHKAI 582 GTGCAC G[AT]GC[AT]^C 1009 GAGCAC BspMI 92 ACCTGC ACCTGC(4/8) 1200 ACCTGC BsrFI 604 ACCGGC [GA]^CCGG[CT] 1190 GCCGGT Bsu36I 325 CCTAAGG CC^T[ACGT]AGG 1267 CCTGAGG Cfr10I 604 ACCGGC [GA]^CCGG[CT] 1190 GCCGGT EarI 505 GAAGAG CTCTTC(1/4) 593 GAAGAG Eco81I 325 CCTAAGG CC^T[ACGT]AGG 1267 CCTGAGG EcoT14I 35 CCTTGG C^C[AT][AT]GG 1047 CCTAGG EcoT22I 1222 ATGCAT ATGCA^T 1304 ATGCAT HaeII 561 GGCGCT [GA]GCGC^[CT] 674 AGCGCC HinfI 475 GAGTC G^A[ACGT]TC 1279 GACTC Ksp632I 505 GAAGAG CTCTTC(1/4) 593 GAAGAG MaeII 112 ACGT A^CGT 483 ACGT NsiI 1222 ATGCAT ATGCA^T 1304 ATGCAT PleI 1279 GACTC GAGTC(4/5) 475 GAGTC PstI 805 CTGCAG CTGCA^G 1202 CTGCAG SbfI 804 CCTGCAGG CCTGCA^GG 1201 CCTGCAGG SfcI 805 CTGCAG C^T[GA][CT]AG 1202 CTGCAG Sse8387I 804 CCTGCAGG CCTGCA^GG 1201 CCTGCAGG StyI 35 CCTTGG C^C[AT][AT]GG 1047 CCTAGG ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AfaI AluI AlwI ApaI AvaI AvaII BanI BanII BbvI BcnI BmyI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TseI Tsp45I TspRI XhoII