(Circular) (Six-base) MAPSORT of: pFN21AE4492.seq Check: 3669 from: 1 to: 6241 pFN21AE4492 6241 bp With 182 enzymes: SgfI * March 28, 2012 13:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2106 2106 Size: 6241 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4051 278 Size: 53 83 186 936 2515 2468 Fragments arranged by size: 2515 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2483 3584 1937 Size: 546 1101 4594 Fragments arranged by size: 4594 1101 546 Acc65I G'GTAC_C Cuts at: 3562 3562 Size: 6241 AclI AA'CG_TT Cuts at: 1563 4368 4741 1563 Size: 2805 373 3063 Fragments arranged by size: 3063 2805 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2474 2949 3533 4304 5688 5956 1632 Size: 842 475 584 771 1384 268 1917 Fragments arranged by size: 1917 1384 842 771 584 475 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6044 AflIII A'CryG_T Cuts at: 1203 2486 5140 6117 1203 Size: 1283 2654 977 1327 Fragments arranged by size: 2654 1327 1283 977 AgeI A'CCGG_T Cuts at: 5072 5072 Size: 6241 AhdI GACnn_n'nnGTC Cuts at: 4970 4970 Size: 6241 AleI CACnn'nnGTG Cuts at: 1166 3185 1166 Size: 2019 4222 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3252 3396 1184 Size: 2068 144 4029 Fragments arranged by size: 4029 2068 144 AlwNI CAG_nnn'CTG Cuts at: 3363 3984 5556 3363 Size: 621 1572 4048 Fragments arranged by size: 4048 1572 621 ApaI G_GGCC'C Cuts at: 2227 2400 3357 3527 2227 Size: 173 957 170 4941 Fragments arranged by size: 4941 957 173 170 ApaLI G'TGCA_C Cuts at: 2587 4298 5454 2587 Size: 1711 1156 3374 Fragments arranged by size: 3374 1711 1156 ApoI r'AATT_y Cuts at: 1493 2138 3550 3776 3928 6037 1493 Size: 645 1412 226 152 2109 1697 Fragments arranged by size: 2109 1697 1412 645 226 152 AseI AT'TA_AT Cuts at: 160 1032 4795 160 Size: 872 3763 1606 Fragments arranged by size: 3763 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6241 AvaI C'yCGr_G Cuts at: 1870 1943 2180 2520 2883 3566 1870 Size: 73 237 340 363 683 4545 Fragments arranged by size: 4545 683 363 340 237 73 AvrII C'CTAG_G Cuts at: 3052 3052 Size: 6241 BamHI G'GATC_C Cuts at: 3571 3938 3571 Size: 367 5874 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2850 2879 3562 5017 Size: 325 217 627 221 842 29 683 1455 Cuts at: 5017 618 Size: 1842 Fragments arranged by size: 1842 1455 842 683 627 325 221 217 29 BanII G_rGCy'C Cuts at: 1391 2227 2400 3357 3527 3560 1391 Size: 836 173 957 170 33 4072 Fragments arranged by size: 4072 957 836 173 170 33 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5270 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3005 3039 4432 4466 1903 Size: 34 1068 34 1393 34 3678 Fragments arranged by size: 3678 1393 1068 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4133 5343 4133 Size: 1210 5031 BclI T'GATC_A Cuts at: 1548 1548 Size: 6241 BfrBI ATG'CAT Cuts at: 3229 3311 3229 Size: 82 6159 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4852 136 Size: 107 122 71 631 605 3180 1525 Fragments arranged by size: 3180 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6236 6236 Size: 6241 BlpI GC'TnA_GC Cuts at: 3167 3658 3167 Size: 491 5750 Bme1580I G_kGCm'C Cuts at: 1790 2013 2227 2400 2591 2884 3150 3357 3527 Size: 223 214 173 191 293 266 207 170 Cuts at: 3527 4302 5458 1790 Size: 775 1156 2573 Fragments arranged by size: 2573 1156 775 293 266 223 214 207 191 173 170 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6241 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3530 3972 4930 Size: 416 53 83 399 24 2113 442 958 Cuts at: 4930 442 Size: 1753 Fragments arranged by size: 2113 1753 958 442 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6241 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2345 4901 1239 Size: 1106 2556 2579 Fragments arranged by size: 2579 2556 1106 Bpu10I CC'TnA_GC Cuts at: 2027 2124 3012 2027 Size: 97 888 5256 Fragments arranged by size: 5256 888 97 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2069 2411 4366 5231 5529 2069 Size: 342 1955 865 298 2781 Fragments arranged by size: 2781 1955 865 342 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3204 4904 882 Size: 617 1705 1700 2219 Fragments arranged by size: 2219 1705 1700 617 BsaAI yAC'GTr Cuts at: 493 1204 2489 4032 493 Size: 711 1285 1543 2702 Fragments arranged by size: 2702 1543 1285 711 BsaBI GATnn'nnATC Cuts at: 6235 6235 Size: 6241 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4048 4430 5065 275 Size: 53 83 186 936 2515 382 635 1451 Fragments arranged by size: 2515 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2844 4674 5072 5346 5493 5904 2844 Size: 1830 398 274 147 411 3181 Fragments arranged by size: 3181 1830 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2516 2546 1178 Size: 30 165 30 1113 30 4873 Fragments arranged by size: 4873 1113 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2026 2616 3050 1186 Size: 840 590 434 4377 Fragments arranged by size: 4377 840 590 434 BseYI C'CCAG_C Cuts at: 2012 3350 3471 5444 5940 2012 Size: 1338 121 1973 496 2313 Fragments arranged by size: 2313 1973 1338 496 121 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5668 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3691 4452 5480 1540 Size: 275 186 1690 761 1028 2301 Fragments arranged by size: 2301 1690 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2591 3018 4302 4387 5458 2591 Size: 427 1284 85 1071 3374 Fragments arranged by size: 3374 1284 1071 427 85 BsmI GAATG_Cn' Cuts at: 3313 3752 3845 3313 Size: 439 93 5709 Fragments arranged by size: 5709 439 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2186 2186 Size: 6241 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2227 2400 2591 2884 3018 3150 Size: 399 223 214 173 191 293 134 132 Cuts at: 3150 3357 3527 3560 4302 4387 5458 1391 Size: 207 170 33 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 293 223 214 207 191 173 170 134 132 85 33 BspEI T'CCGG_A Cuts at: 5904 5904 Size: 6241 BspHI T'CATG_A Cuts at: 2580 4130 5860 2580 Size: 1550 1730 2961 Fragments arranged by size: 2961 1730 1550 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2106 3214 3596 844 Size: 1262 1108 382 3489 Fragments arranged by size: 3489 1262 1108 382 BsrBI CCG'CTC Cuts at: 1394 1823 2797 4128 1394 Size: 429 974 1331 3507 Fragments arranged by size: 3507 1331 974 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4736 4910 65 Size: 1183 3488 174 1396 Fragments arranged by size: 3488 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2609 3195 4885 5072 1700 Size: 110 799 586 1690 187 2869 Fragments arranged by size: 2869 1690 799 586 187 110 BsrGI T'GTAC_A Cuts at: 96 2313 96 Size: 2217 4024 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6241 BssSI C'ACGA_G Cuts at: 4301 5313 4301 Size: 1012 5229 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6241 BstBI TT'CG_AA Cuts at: 5045 5045 Size: 6241 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6241 BstXI CCAn_nnnn'nTGG Cuts at: 2369 2369 Size: 6241 BstYI r'GATC_y Cuts at: 1972 2184 2835 3048 3571 3938 4323 4340 5792 Size: 212 651 213 523 367 385 17 1452 Cuts at: 5792 5878 5890 6236 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 651 523 385 367 346 213 212 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2331 3273 2331 Size: 942 5299 BtgI C'CryG_G Cuts at: 513 1065 2004 2320 513 Size: 552 939 316 4434 Fragments arranged by size: 4434 939 552 316 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2565 487 Size: 683 56 1339 4163 Fragments arranged by size: 4163 1339 683 56 BtsI GCAGTG_nn' Cuts at: 3757 4551 4571 3757 Size: 794 20 5427 Fragments arranged by size: 5427 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6241 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6206 DraI TTT'AAA Cuts at: 3545 3545 Size: 6241 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3275 5248 809 Size: 507 1959 1973 1802 Fragments arranged by size: 1973 1959 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2466 2734 3193 Size: 54 1425 50 275 18 636 268 459 Cuts at: 3193 3688 4577 6206 8 Size: 495 889 1629 43 Fragments arranged by size: 1629 1425 889 636 495 459 275 268 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3688 1537 Size: 275 1876 4090 Fragments arranged by size: 4090 1876 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2505 2593 4171 1353 Size: 1152 88 1578 3423 Fragments arranged by size: 3423 1578 1152 88 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3119 4828 5202 5348 387 Size: 1064 138 1530 1709 374 146 1280 Fragments arranged by size: 1709 1530 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2345 2474 2949 3533 4304 4901 5688 Size: 393 713 129 475 584 771 597 787 Cuts at: 5688 5956 1239 Size: 268 1524 Fragments arranged by size: 1524 787 771 713 597 584 475 393 268 129 EcoO109I rG'GnC_Cy Cuts at: 2147 2326 2335 2396 3524 2147 Size: 179 9 61 1128 4864 Fragments arranged by size: 4864 1128 179 61 9 EcoRI G'AATT_C Cuts at: 3550 3928 3550 Size: 378 5863 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2608 3142 3514 2608 Size: 534 372 5335 Fragments arranged by size: 5335 534 372 FspI TGC'GCA Cuts at: 4747 4747 Size: 6241 HaeII r_GCGC'y Cuts at: 1678 2570 2683 5388 1678 Size: 892 113 2705 2531 Fragments arranged by size: 2705 2531 892 113 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2163 2195 2516 2548 3024 Size: 32 135 32 591 32 321 32 476 Cuts at: 3024 3056 3182 3214 3296 3328 4882 4914 4956 Size: 32 126 32 82 32 1554 32 42 Cuts at: 4956 4988 1373 Size: 32 2626 Fragments arranged by size: 2626 1554 591 476 321 135 126 82 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3585 3831 669 Size: 988 281 1647 246 3079 Fragments arranged by size: 3079 1647 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6241 HpaI GTT'AAC Cuts at: 3831 3831 Size: 6241 KpnI G_GTAC'C Cuts at: 3566 3566 Size: 6241 MfeI C'AATT_G Cuts at: 3840 3840 Size: 6241 MluI A'CGCG_T Cuts at: 6117 6117 Size: 6241 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5355 5539 1305 Size: 4050 184 2007 Fragments arranged by size: 4050 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4419 Fragments arranged by size: 4419 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2445 2579 2942 3185 3234 3410 3464 Size: 648 1279 134 363 243 49 176 54 Cuts at: 3464 4199 4558 4717 518 Size: 735 359 159 2042 Fragments arranged by size: 2042 1279 735 648 363 359 243 176 159 134 54 49 MspA1I CmG'CkG Cuts at: 1606 3657 4334 5482 5727 1606 Size: 2051 677 1148 245 2120 Fragments arranged by size: 2120 2051 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4750 Fragments arranged by size: 4750 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6241 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6241 NotI GC'GGCC_GC Cuts at: 3688 3688 Size: 6241 NsiI A_TGCA'T Cuts at: 3231 3313 3231 Size: 82 6159 NspI r_CATG'y Cuts at: 1132 1229 2177 3599 5144 1132 Size: 97 948 1422 1545 2229 Fragments arranged by size: 2229 1545 1422 948 97 PasI CC'CwG_GG Cuts at: 1348 2144 3527 1348 Size: 796 1383 4062 Fragments arranged by size: 4062 1383 796 PciI A'CATG_T Cuts at: 5140 5140 Size: 6241 PflMI CCAn_nnn'nTGG Cuts at: 1108 2160 1108 Size: 1052 5189 PfoI T'CCnGG_A Cuts at: 2187 2862 2187 Size: 675 5566 PmeI GTTT'AAAC Cuts at: 3545 3545 Size: 6241 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4310 4342 5823 5855 4310 Size: 32 1481 32 4696 Fragments arranged by size: 4696 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2326 2326 Size: 6241 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6241 PsiI TTA'TAA Cuts at: 3811 3811 Size: 6241 PspOMI G'GGCC_C Cuts at: 2223 2396 3353 3523 2223 Size: 173 957 170 4941 Fragments arranged by size: 4941 957 173 170 PstI C_TGCA'G Cuts at: 830 2814 3211 3593 830 Size: 1984 397 382 3478 Fragments arranged by size: 3478 1984 397 382 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6241 SalI G'TCGA_C Cuts at: 1936 3583 1936 Size: 1647 4594 SanDI GG'GwC_CC Cuts at: 2326 2326 Size: 6241 SbfI CC_TGCA'GG Cuts at: 2814 3211 3593 2814 Size: 397 382 5462 Fragments arranged by size: 5462 397 382 ScaI AGT'ACT Cuts at: 4489 6201 4489 Size: 1712 4529 SexAI A'CCwGG_T Cuts at: 2781 2781 Size: 6241 SfcI C'TryA_G Cuts at: 826 1045 2810 3207 3589 4724 5405 5596 826 Size: 219 1765 397 382 1135 681 191 1471 Fragments arranged by size: 1765 1471 1135 681 397 382 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6241 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6241 SmaI CCC'GGG Cuts at: 1872 2885 3568 1872 Size: 1013 683 4545 Fragments arranged by size: 4545 1013 683 SmlI C'TyrA_G Cuts at: 820 1017 1943 2084 2180 2390 4345 5246 5508 Size: 197 926 141 96 210 1955 901 262 Cuts at: 5508 820 Size: 1553 Fragments arranged by size: 1955 1553 926 901 262 210 197 141 96 SnaBI TAC'GTA Cuts at: 493 4032 493 Size: 3539 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6241 SphI G_CATG'C Cuts at: 1132 3599 1132 Size: 2467 3774 SspI AAT'ATT Cuts at: 5 52 4165 5 Size: 47 4113 2081 Fragments arranged by size: 4113 2081 47 StuI AGG'CCT Cuts at: 1517 2379 1517 Size: 862 5379 StyI C'CwwG_G Cuts at: 513 1065 2004 2040 3052 3680 513 Size: 552 939 36 1012 628 3074 Fragments arranged by size: 3074 1012 939 628 552 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2171 2262 2867 4268 4285 4438 4623 Size: 1255 591 91 605 1401 17 153 185 Cuts at: 4623 5068 325 Size: 445 1498 Fragments arranged by size: 1498 1401 1255 605 591 445 185 153 91 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2313 4487 6199 Size: 274 80 33 51 1443 336 2174 1712 Cuts at: 6199 96 Size: 138 Fragments arranged by size: 2174 1712 1443 336 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6201 6233 6201 Size: 32 6209 Tth111I GACn'n_nGTC Cuts at: 1316 2783 1316 Size: 1467 4774 XbaI T'CTAG_A Cuts at: 3577 3577 Size: 6241 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2165 2165 Size: 6241 XhoI C'TCGA_G Cuts at: 1943 2180 1943 Size: 237 6004 XmaI C'CCGG_G Cuts at: 1870 2883 3566 1870 Size: 1013 683 4545 Fragments arranged by size: 4545 1013 683 XmnI GAAnn'nnTTC Cuts at: 4370 4370 Size: 6241 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4049 276 Size: 53 83 186 936 2515 2468 Fragments arranged by size: 2515 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BbvCI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SfiI SfoI SrfI SwaI