(Linear) (Six-base) MAPSORT of: KIEE4492.seq Check: 3191 from: 1 to: 1536 KIEE4492.orf With 182 enzymes: SgfI * March 22, 2012 18:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 101 1536 Size: 101 1435 AccI GT'mk_AC Cuts at: 0 478 1536 Size: 478 1058 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 469 944 1528 1536 Size: 469 475 584 8 Fragments arranged by size: 584 475 469 8 AflIII A'CryG_T Cuts at: 0 481 1536 Size: 481 1055 AleI CACnn'nnGTG Cuts at: 0 1180 1536 Size: 1180 356 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1247 1391 1536 Size: 1247 144 145 Fragments arranged by size: 1247 145 144 AlwNI CAG_nnn'CTG Cuts at: 0 1358 1536 Size: 1358 178 ApaI G_GGCC'C Cuts at: 0 222 395 1352 1522 1536 Size: 222 173 957 170 14 Fragments arranged by size: 957 222 173 170 14 ApaLI G'TGCA_C Cuts at: 0 582 1536 Size: 582 954 ApoI r'AATT_y Cuts at: 0 133 1536 Size: 133 1403 AvaI C'yCGr_G Cuts at: 0 175 515 878 1536 Size: 175 340 363 658 Fragments arranged by size: 658 363 340 175 AvrII C'CTAG_G Cuts at: 0 1047 1536 Size: 1047 489 BanI G'GyrC_C Cuts at: 0 3 845 874 1536 Size: 3 842 29 662 Fragments arranged by size: 842 662 29 3 BanII G_rGCy'C Cuts at: 0 222 395 1352 1522 1536 Size: 222 173 957 170 14 Fragments arranged by size: 957 222 173 170 14 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1000 1034 1536 Size: 1000 34 502 Fragments arranged by size: 1000 502 34 BfrBI ATG'CAT Cuts at: 0 1224 1306 1536 Size: 1224 82 230 Fragments arranged by size: 1224 230 82 BlpI GC'TnA_GC Cuts at: 0 1162 1536 Size: 1162 374 Bme1580I G_kGCm'C Cuts at: 0 8 222 395 586 879 1145 1352 1522 Size: 8 214 173 191 293 266 207 170 Cuts at: 1522 1536 Size: 14 Fragments arranged by size: 293 266 214 207 191 173 170 14 8 BmrI ACTGGGnnnn_n' Cuts at: 0 1525 1536 Size: 1525 11 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 340 1536 Size: 340 1196 Bpu10I CC'TnA_GC Cuts at: 0 22 119 1007 1536 Size: 22 97 888 529 Fragments arranged by size: 888 529 97 22 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 406 1536 Size: 64 342 1130 Fragments arranged by size: 1130 342 64 BsaI GGTCTCn'nnnn_ Cuts at: 0 1199 1536 Size: 1199 337 BsaAI yAC'GTr Cuts at: 0 484 1536 Size: 484 1052 BsaWI w'CCGG_w Cuts at: 0 839 1536 Size: 839 697 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 511 541 1536 Size: 511 30 995 Fragments arranged by size: 995 511 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 21 611 1045 1536 Size: 21 590 434 491 Fragments arranged by size: 590 491 434 21 BseYI C'CCAG_C Cuts at: 0 7 1345 1466 1536 Size: 7 1338 121 70 Fragments arranged by size: 1338 121 70 7 BsiHKAI G_wGCw'C Cuts at: 0 586 1013 1536 Size: 586 427 523 Fragments arranged by size: 586 523 427 BsmI GAATG_Cn' Cuts at: 0 1308 1536 Size: 1308 228 BsmBI CGTCTCn'nnnn_ Cuts at: 0 181 1536 Size: 181 1355 Bsp1286I G_dGCh'C Cuts at: 0 8 222 395 586 879 1013 1145 1352 Size: 8 214 173 191 293 134 132 207 Cuts at: 1352 1522 1536 Size: 170 14 Fragments arranged by size: 293 214 207 191 173 170 134 132 14 8 BspHI T'CATG_A Cuts at: 0 575 1536 Size: 575 961 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 101 1209 1536 Size: 101 1108 327 Fragments arranged by size: 1108 327 101 BsrBI CCG'CTC Cuts at: 0 792 1536 Size: 792 744 BsrFI r'CCGG_y Cuts at: 0 604 1190 1536 Size: 604 586 346 Fragments arranged by size: 604 586 346 BsrGI T'GTAC_A Cuts at: 0 308 1536 Size: 308 1228 BstXI CCAn_nnnn'nTGG Cuts at: 0 364 1536 Size: 364 1172 BstYI r'GATC_y Cuts at: 0 179 830 1043 1536 Size: 179 651 213 493 Fragments arranged by size: 651 493 213 179 Bsu36I CC'TnA_GG Cuts at: 0 326 1268 1536 Size: 326 942 268 Fragments arranged by size: 942 326 268 BtgI C'CryG_G Cuts at: 0 315 1536 Size: 315 1221 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 560 1536 Size: 560 976 DrdI GACnn_nn'nnGTC Cuts at: 0 1270 1536 Size: 1270 266 EaeI y'GGCC_r Cuts at: 0 461 729 1188 1536 Size: 461 268 459 348 Fragments arranged by size: 461 459 348 268 EarI CTCTTCn'nnn_ Cuts at: 0 500 588 1536 Size: 500 88 948 Fragments arranged by size: 948 500 88 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1114 1536 Size: 1114 422 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 340 469 944 1528 1536 Size: 340 129 475 584 8 Fragments arranged by size: 584 475 340 129 8 EcoO109I rG'GnC_Cy Cuts at: 0 142 321 330 391 1519 1536 Size: 142 179 9 61 1128 17 Fragments arranged by size: 1128 179 142 61 17 9 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 603 1137 1509 1536 Size: 603 534 372 27 Fragments arranged by size: 603 534 372 27 HaeII r_GCGC'y Cuts at: 0 565 678 1536 Size: 565 113 858 Fragments arranged by size: 858 565 113 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 158 190 511 543 1019 1051 1177 1209 Size: 158 32 321 32 476 32 126 32 Cuts at: 1209 1291 1323 1536 Size: 82 32 213 Fragments arranged by size: 476 321 213 158 126 82 32 32 32 32 32 MslI CAynn'nnrTG Cuts at: 0 440 574 937 1180 1229 1405 1459 1536 Size: 440 134 363 243 49 176 54 77 Fragments arranged by size: 440 363 243 176 134 77 54 49 NsiI A_TGCA'T Cuts at: 0 1226 1308 1536 Size: 1226 82 228 Fragments arranged by size: 1226 228 82 NspI r_CATG'y Cuts at: 0 172 1536 Size: 172 1364 PasI CC'CwG_GG Cuts at: 0 139 1522 1536 Size: 139 1383 14 Fragments arranged by size: 1383 139 14 PflMI CCAn_nnn'nTGG Cuts at: 0 155 1536 Size: 155 1381 PfoI T'CCnGG_A Cuts at: 0 182 857 1536 Size: 182 675 679 Fragments arranged by size: 679 675 182 PpuMI rG'GwC_Cy Cuts at: 0 321 1536 Size: 321 1215 PspOMI G'GGCC_C Cuts at: 0 218 391 1348 1518 1536 Size: 218 173 957 170 18 Fragments arranged by size: 957 218 173 170 18 PstI C_TGCA'G Cuts at: 0 809 1206 1536 Size: 809 397 330 Fragments arranged by size: 809 397 330 SanDI GG'GwC_CC Cuts at: 0 321 1536 Size: 321 1215 SbfI CC_TGCA'GG Cuts at: 0 809 1206 1536 Size: 809 397 330 Fragments arranged by size: 809 397 330 SexAI A'CCwGG_T Cuts at: 0 776 1536 Size: 776 760 SfcI C'TryA_G Cuts at: 0 805 1202 1536 Size: 805 397 334 Fragments arranged by size: 805 397 334 SmaI CCC'GGG Cuts at: 0 880 1536 Size: 880 656 SmlI C'TyrA_G Cuts at: 0 79 175 385 1536 Size: 79 96 210 1151 Fragments arranged by size: 1151 210 96 79 StuI AGG'CCT Cuts at: 0 374 1536 Size: 374 1162 StyI C'CwwG_G Cuts at: 0 35 1047 1536 Size: 35 1012 489 Fragments arranged by size: 1012 489 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 166 257 862 1536 Size: 166 91 605 674 Fragments arranged by size: 674 605 166 91 TatI w'GTAC_w Cuts at: 0 308 1536 Size: 308 1228 Tth111I GACn'n_nGTC Cuts at: 0 778 1536 Size: 778 758 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 160 1536 Size: 160 1376 XhoI C'TCGA_G Cuts at: 0 175 1536 Size: 175 1361 XmaI C'CCGG_G Cuts at: 0 878 1536 Size: 878 658 Enzymes that do cut: AarI AccI AcuI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI AvaI AvrII BanI BanII BcgI BfrBI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BstXI BstYI Bsu36I BtgI BtgZI DrdI EaeI EarI EciI Eco57MI EcoO109I FalI HaeII Hin4I MslI NsiI NspI PasI PflMI PfoI PpuMI PspOMI PstI SanDI SbfI SexAI SfcI SmaI SmlI StuI StyI TaqII TatI Tth111I XcmI XhoI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AloI AscI AseI AsiSI BaeI BamHI BbeI BbsI BbvCI BciVI BclI BglI BglII BmgBI BmtI BplI BsaBI BsaHI BsgI BsiEI BsiWI BspEI BsrDI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtsI ClaI CspCI DraI DraIII EagI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SapI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI XmnI ZraI