Restriction map for
pFN21AB7985
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII Acc65I AccI AcyI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BbeI BbrPI BciVI
BclI BfrI BlnI BsaBI BsaHI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HindIII
HpaI KasI KpnI MamI MfeI
MluNI MscI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RcaI RsrII
SacI SalI SapI ScaI SexAI
SfoI SfuI SgrAI SnaBI SphI
SspBI SspI StuI SwaI TfiI
Tth111I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccIII 740 TCCGGA T^CCGGA
AclI 835 AACGTT AA^CGTT
AlwNI 929 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 736 GTGCTC G[AT]GC[AT]^C
BamHI 345 GGATCC G^GATCC
BanI 40 GGCACC G^G[CT][GA]CC
BcgI 912 CGAATTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1195 GCCCTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 769 AGATCT A^GATCT
BlpI 399 GCTCAGC GC^T[ACGT]AGC
BpmI 1084 CTGGAG CTGGAG(16/14)
Bpu1102I 399 GCTCAGC GC^T[ACGT]AGC
BsaAI 898 TACGTG [CT]AC^GT[GA]
BsaI 416 GAGACC GGTCTC(1/5)
BsaWI 740 TCCGGA [AT]^CCGG[AT]
BseAI 740 TCCGGA T^CCGGA
BsiHKAI 736 GTGCTC G[AT]GC[AT]^C
BspEI 740 TCCGGA T^CCGGA
BspMI 1031 ACCTGC ACCTGC(4/8)
BsrDI 542 GCAATG GCAATG(2/0)
BstXI 288 CCAGGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 399 GCTCAGC GC^T[ACGT]AGC
EaeI 273 CGGCCA [CT]^GGCC[GA]
EarI 580 CTCTTC CTCTTC(1/4)
HaeII 103 AGCGCC [GA]GCGC^[CT]
HgaI 718 GCGTC GACGC(5/10)
HincII 208 GTCAAC GT[CT]^[GA]AC
HindII 208 GTCAAC GT[CT]^[GA]AC
Ksp632I 580 CTCTTC CTCTTC(1/4)
MluI 716 ACGCGT A^CGCGT
MroI 740 TCCGGA T^CCGGA
MslI 701 CACCCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
Psp1406I 835 AACGTT AA^CGTT
SbfI 811 CCTGCAGG CCTGCA^GG
SfaNI 1094 GCATC GCATC(5/9)
SfiI 1194 GGCCCTCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 474 CCCGGG CCC^GGG
SmlI 1198 CTCAAG C^T[CT][GA]AG
SpeI 855 ACTAGT A^CTAGT
Sse8387I 811 CCTGCAGG CCTGCA^GG
XcmI 1073 CCAACCCCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 474 CCCGGG C^CCGGG
XmaI 474 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 913 GAATTT [GA]^AATT[CT]
937 GAATTT
AflIII 716 ACGCGT A^C[GA][CT]GT
899 ACGTGT
ApoI 913 GAATTT [GA]^AATT[CT]
937 GAATTT
AvaI 474 CCCGGG C^[CT]CG[GA]G
703 CCCGAG
BanII 298 GGGCTC G[GA]GC[CT]^C
1087 GAGCCC
BbsI 469 GAAGAC GAAGAC(2/6)
610 GTCTTC
BbvCI 642 CCTCAGC CCTCAGC(-5/-2)
1221 CCTCAGC
BfaI 856 CTAG C^TAG
880 CTAG
BpuAI 469 GAAGAC GAAGAC(2/6)
610 GTCTTC
BsmBI 549 CGTCTC CGTCTC(1/5)
819 GAGACG
BsoBI 474 CCCGGG C^[CT]CG[GA]G
703 CCCGAG
BsrI 786 ACTGG ACTGG(1/-1)
183 CCAGT
BssSI 99 CACGAG CACGAG(-5/-1)
370 CACGAG
CfoI 104 GCGC GCG^C
397 GCGC
EcoT14I 23 CCATGG C^C[AT][AT]GG
1205 CCATGG
HhaI 104 GCGC GCG^C
397 GCGC
HinP1I 104 GCGC G^CGC
397 GCGC
HinfI 292 GAGTC G^A[ACGT]TC
410 GACTC
KspI 1148 CCGCGG CCGC^GG
1160 CCGCGG
MaeI 856 CTAG C^TAG
880 CTAG
NcoI 23 CCATGG C^CATGG
1205 CCATGG
NspI 462 GCATGT [GA]CATG^[CT]
989 ACATGC
PleI 410 GACTC GAGTC(4/5)
292 GAGTC
PpuMI 374 AGGACCT [GA]G^G[AT]CC[CT]
455 AGGTCCC
PstI 812 CTGCAG CTGCA^G
1174 CTGCAG
PvuII 70 CAGCTG CAG^CTG
331 CAGCTG
SacII 1148 CCGCGG CCGC^GG
1160 CCGCGG
StyI 23 CCATGG C^C[AT][AT]GG
1205 CCATGG
TspRI 78 CACTG CA[GC]TG[ACGT][ACGT]^
1266 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BbvI BcnI BmyI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstUI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfcI TaqI
Tru9I TseI Tsp45I Tsp509I TthHB8I
XhoII