Restriction map for
pFN21AB7985
(Circular) (Six-base) MAPSORT of: pFN21AB7985.seq Check: 1696 from: 1 to: 5998
pFN21AB7985 5998 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3808 278
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3341 1937
Size: 1404 4594
Acc65I G'GTAC_C
Cuts at: 3319 3319
Size: 5998
AclI AA'CG_TT
Cuts at: 1563 2841 4125 4498 1563
Size: 1278 1284 373 3063
Fragments arranged by size:
3063 1284 1278 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4061 5445 5713 1632
Size: 2429 1384 268 1917
Fragments arranged by size:
2429 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5801
AflIII A'CryG_T
Cuts at: 1203 2721 2904 4897 5874 1203
Size: 1518 183 1993 977 1327
Fragments arranged by size:
1993 1518 1327 977 183
AgeI A'CCGG_T
Cuts at: 4829 4829
Size: 5998
AhdI GACnn_n'nnGTC
Cuts at: 4727 4727
Size: 5998
AleI CACnn'nnGTG
Cuts at: 1166 2710 1166
Size: 1544 4454
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5998
AlwNI CAG_nnn'CTG
Cuts at: 2939 3741 5313 2939
Size: 802 1572 3624
Fragments arranged by size:
3624 1572 802
ApaLI G'TGCA_C
Cuts at: 4055 5211 4055
Size: 1156 4842
ApoI r'AATT_y
Cuts at: 1493 2918 2942 3307 3533 3685 5794 1493
Size: 1425 24 365 226 152 2109 1697
Fragments arranged by size:
2109 1697 1425 365 226 152 24
AseI AT'TA_AT
Cuts at: 160 1032 4552 160
Size: 872 3520 1606
Fragments arranged by size:
3520 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
AvaI C'yCGr_G
Cuts at: 1870 1943 2479 2708 3323 1870
Size: 73 536 229 615 4545
Fragments arranged by size:
4545 615 536 229 73
BamHI G'GATC_C
Cuts at: 2350 3328 3695 2350
Size: 978 367 4653
Fragments arranged by size:
4653 978 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 3319 4774 618
Size: 325 217 627 258 1274 1455 1842
Fragments arranged by size:
1842 1455 1274 627 325 258 217
BanII G_rGCy'C
Cuts at: 1391 2307 3096 3317 1391
Size: 916 789 221 4072
Fragments arranged by size:
4072 916 789 221
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2481 2608 928
Size: 971 582 127 4318
Fragments arranged by size:
4318 971 582 127
BbvCI CC'TCA_GC
Cuts at: 2648 3227 2648
Size: 579 5419
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2906 2940 4189 4223 1903
Size: 34 969 34 1249 34 3678
Fragments arranged by size:
3678 1249 969 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3890 5100 3890
Size: 1210 4788
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5998
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3206 4609 136
Size: 107 122 71 631 605 1534 1403 1525
Fragments arranged by size:
1534 1525 1403 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2774 5993 2774
Size: 3219 2779
BlpI GC'TnA_GC
Cuts at: 2405 3415 2405
Size: 1010 4988
Bme1580I G_kGCm'C
Cuts at: 1790 2084 4059 5215 1790
Size: 294 1975 1156 2573
Fragments arranged by size:
2573 1975 1156 294
BmgBI CAC'GTC
Cuts at: 1318 2212 1318
Size: 894 5104
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2182 3729 4687
Size: 416 53 83 399 24 765 1547 958
Cuts at: 4687 442
Size: 1753
Fragments arranged by size:
1753 1547 958 765 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3110 4658 1239
Size: 1871 1548 2579
Fragments arranged by size:
2579 1871 1548
Bpu10I CC'TnA_GC
Cuts at: 2410 2648 3045 3227 2410
Size: 238 397 182 5181
Fragments arranged by size:
5181 397 238 182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3188 4123 4988 5286 3188
Size: 935 865 298 3900
Fragments arranged by size:
3900 935 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2415 4661 882
Size: 617 916 2246 2219
Fragments arranged by size:
2246 2219 916 617
BsaAI yAC'GTr
Cuts at: 493 1204 2905 3789 493
Size: 711 1701 884 2702
Fragments arranged by size:
2702 1701 884 711
BsaBI GATnn'nnATC
Cuts at: 5992 5992
Size: 5998
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3805 4187 4822 275
Size: 53 83 186 936 2272 382 635 1451
Fragments arranged by size:
2272 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2745 4431 4829 5103 5250 5661 2745
Size: 1686 398 274 147 411 3082
Fragments arranged by size:
3082 1686 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2862 2892 3116 3146 1178
Size: 30 165 30 1459 30 224 30 4030
Fragments arranged by size:
4030 1459 224 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2471 2636 3296 1186
Size: 1285 165 660 3888
Fragments arranged by size:
3888 1285 660 165
BseYI C'CCAG_C
Cuts at: 2073 2077 2247 2731 5201 5697 2073
Size: 4 170 484 2470 496 2374
Fragments arranged by size:
2470 2374 496 484 170 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2225 2294 2372 2708 1163
Size: 573 489 69 78 336 4453
Fragments arranged by size:
4453 573 489 336 78 69
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3448 4209 5237 1540
Size: 275 186 1447 761 1028 2301
Fragments arranged by size:
2301 1447 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2745 4059 4144 5215 2745
Size: 1314 85 1071 3528
Fragments arranged by size:
3528 1314 1071 85
BsmI GAATG_Cn'
Cuts at: 3509 3602 3509
Size: 93 5905
BsmBI CGTCTCn'nnnn_
Cuts at: 2560 2818 2560
Size: 258 5740
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2084 2307 2745 3096 3317 4059 4144
Size: 399 294 223 438 351 221 742 85
Cuts at: 4144 5215 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 438 399 351 294 223
221 85
BspEI T'CCGG_A
Cuts at: 2745 5661 2745
Size: 2916 3082
BspHI T'CATG_A
Cuts at: 3887 5617 3887
Size: 1730 4268
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3045 3353 844
Size: 2201 308 3489
Fragments arranged by size:
3489 2201 308
BsrBI CCG'CTC
Cuts at: 1394 1823 3885 1394
Size: 429 2062 3507
Fragments arranged by size:
3507 2062 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2554 4493 4667 65
Size: 1183 1306 1939 174 1396
Fragments arranged by size:
1939 1396 1306 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4642 4829 1700
Size: 110 2832 187 2869
Fragments arranged by size:
2869 2832 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5998
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5998
BssSI C'ACGA_G
Cuts at: 2104 2375 4058 5070 2104
Size: 271 1683 1012 3032
Fragments arranged by size:
3032 1683 1012 271
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5998
BstBI TT'CG_AA
Cuts at: 4802 4802
Size: 5998
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5998
BstXI CCAn_nnnn'nTGG
Cuts at: 2300 2300
Size: 5998
BstYI r'GATC_y
Cuts at: 1972 2197 2350 2774 3328 3695 4080 4097 5549
Size: 225 153 424 554 367 385 17 1452
Cuts at: 5549 5635 5647 5993 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 554 424 385 367 346 225
153 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2028 3136 3153 3165 3210 513
Size: 552 963 1108 17 12 45 3301
Fragments arranged by size:
3301 1108 963 552 45 17 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5259
Fragments arranged by size:
5259 683 56
BtsI GCAGTG_nn'
Cuts at: 3277 3514 4308 4328 3277
Size: 237 794 20 4947
Fragments arranged by size:
4947 794 237 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5998
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5963
DraI TTT'AAA
Cuts at: 3302 3302
Size: 5998
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5005 809
Size: 507 3689 1802
Fragments arranged by size:
3689 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2278 3445 4334
Size: 54 1425 50 275 18 448 1167 889
Cuts at: 4334 5963 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1167 889 448 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3445 1537
Size: 275 1633 4090
Fragments arranged by size:
4090 1633 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2591 3928 1353
Size: 1238 1337 3423
Fragments arranged by size:
3423 1337 1238
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2548 2696 2751 4585 4959 5105
Size: 1064 138 959 148 55 1834 374 146
Cuts at: 5105 387
Size: 1280
Fragments arranged by size:
1834 1280 1064 959 374 148 146 138
55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3110 4061 4658 5445 5713 1239
Size: 393 1478 951 597 787 268 1524
Fragments arranged by size:
1524 1478 951 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2380 2461 2519 3199 2380
Size: 81 58 680 5179
Fragments arranged by size:
5179 680 81 58
EcoRI G'AATT_C
Cuts at: 3307 3685 3307
Size: 378 5620
FspI TGC'GCA
Cuts at: 4504 4504
Size: 5998
HaeII r_GCGC'y
Cuts at: 1678 2112 5145 1678
Size: 434 3033 2531
Fragments arranged by size:
3033 2531 434
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2189 2221 2862 2894 4639
Size: 32 135 32 617 32 641 32 1745
Cuts at: 4639 4671 4713 4745 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1745 641 617 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2215 3342 3588 669
Size: 988 281 277 1127 246 3079
Fragments arranged by size:
3079 1127 988 281 277 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5998
HpaI GTT'AAC
Cuts at: 3588 3588
Size: 5998
KpnI G_GTAC'C
Cuts at: 3323 3323
Size: 5998
MfeI C'AATT_G
Cuts at: 3597 3597
Size: 5998
MluI A'CGCG_T
Cuts at: 2721 5874 2721
Size: 3153 2845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2565 5112 5296 1305
Size: 1260 2547 184 2007
Fragments arranged by size:
2547 2007 1260 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4176
Fragments arranged by size:
4176 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2710 3956 4315 4474 518
Size: 648 1544 1246 359 159 2042
Fragments arranged by size:
2042 1544 1246 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2077 2119 2338 2692 3155 3167 3414 4091
Size: 471 42 219 354 463 12 247 677
Cuts at: 4091 5239 5484 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 471 463 354 247 245
219 42 12
NcoI C'CATG_G
Cuts at: 513 1065 2028 3210 513
Size: 552 963 1182 3301
Fragments arranged by size:
3301 1182 963 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5998
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5998
NotI GC'GGCC_GC
Cuts at: 3445 3445
Size: 5998
NspI r_CATG'y
Cuts at: 1132 1229 2471 2998 3356 4901 1132
Size: 97 1242 527 358 1545 2229
Fragments arranged by size:
2229 1545 1242 527 358 97
PasI CC'CwG_GG
Cuts at: 1348 3061 1348
Size: 1713 4285
PciI A'CATG_T
Cuts at: 4897 4897
Size: 5998
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5998
PfoI T'CCnGG_A
Cuts at: 3087 3087
Size: 5998
PmeI GTTT'AAAC
Cuts at: 3302 3302
Size: 5998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4067 4099 5580 5612 4067
Size: 32 1481 32 4453
Fragments arranged by size:
4453 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2380 2461 2380
Size: 81 5917
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5998
PsiI TTA'TAA
Cuts at: 3568 3568
Size: 5998
PstI C_TGCA'G
Cuts at: 830 2821 3183 3350 830
Size: 1991 362 167 3478
Fragments arranged by size:
3478 1991 362 167
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5998
PvuII CAG'CTG
Cuts at: 2077 2338 2077
Size: 261 5737
SacII CC_GC'GG
Cuts at: 3156 3168 3156
Size: 12 5986
SalI G'TCGA_C
Cuts at: 1936 3340 1936
Size: 1404 4594
SbfI CC_TGCA'GG
Cuts at: 2821 3350 2821
Size: 529 5469
ScaI AGT'ACT
Cuts at: 4246 5958 4246
Size: 1712 4286
SfcI C'TryA_G
Cuts at: 826 1045 2085 2224 2365 2817 3179 3346 4481
Size: 219 1040 139 141 452 362 167 1135
Cuts at: 4481 5162 5353 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1040 681 452 362 219 191
167 141 139
SfiI GGCCn_nnn'nGGCC
Cuts at: 3206 3206
Size: 5998
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5998
SmaI CCC'GGG
Cuts at: 1872 2481 3325 1872
Size: 609 844 4545
Fragments arranged by size:
4545 844 609
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3203 4102 5003 5265 820
Size: 197 926 1260 899 901 262 1553
Fragments arranged by size:
1553 1260 926 901 899 262 197
SnaBI TAC'GTA
Cuts at: 493 3789 493
Size: 3296 2702
SpeI A'CTAG_T
Cuts at: 152 2860 152
Size: 2708 3290
SphI G_CATG'C
Cuts at: 1132 3356 1132
Size: 2224 3774
SspI AAT'ATT
Cuts at: 5 52 3922 5
Size: 47 3870 2081
Fragments arranged by size:
3870 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5998
StyI C'CwwG_G
Cuts at: 513 1065 2028 3210 3437 513
Size: 552 963 1182 227 3074
Fragments arranged by size:
3074 1182 963 552 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2280 3066 4025 4042 4195 4380 4825
Size: 1255 700 786 959 17 153 185 445
Cuts at: 4825 325
Size: 1498
Fragments arranged by size:
1498 1255 959 786 700 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2664 4244 5956
Size: 274 80 33 51 1443 687 1580 1712
Cuts at: 5956 96
Size: 138
Fragments arranged by size:
1712 1580 1443 687 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5958 5990 5958
Size: 32 5966
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5998
XbaI T'CTAG_A
Cuts at: 3334 3334
Size: 5998
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3085 3085
Size: 5998
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5998
XmaI C'CCGG_G
Cuts at: 1870 2479 3323 1870
Size: 609 844 4545
Fragments arranged by size:
4545 844 609
XmnI GAAnn'nnTTC
Cuts at: 4127 4127
Size: 5998
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3806 276
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacII SalI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SanDI SapI SexAI SfoI SrfI SwaI