Restriction map for
pFN21AB7985
(Linear) (Six-base) MAPSORT of: KIBB7985.seq Check: 8058 from: 1 to: 1293
KIBB7985.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AclI AA'CG_TT
Cuts at: 0 836 1293
Size: 836 457
AflIII A'CryG_T
Cuts at: 0 716 899 1293
Size: 716 183 394
Fragments arranged by size:
716 394 183
AleI CACnn'nnGTG
Cuts at: 0 705 1293
Size: 705 588
AlwNI CAG_nnn'CTG
Cuts at: 0 934 1293
Size: 934 359
ApoI r'AATT_y
Cuts at: 0 913 937 1293
Size: 913 24 356
Fragments arranged by size:
913 356 24
AvaI C'yCGr_G
Cuts at: 0 474 703 1293
Size: 474 229 590
Fragments arranged by size:
590 474 229
BamHI G'GATC_C
Cuts at: 0 345 1293
Size: 345 948
BanI G'GyrC_C
Cuts at: 0 40 1293
Size: 40 1253
BanII G_rGCy'C
Cuts at: 0 302 1091 1293
Size: 302 789 202
Fragments arranged by size:
789 302 202
BbsI GAAGACnn'nnnn_
Cuts at: 0 476 603 1293
Size: 476 127 690
Fragments arranged by size:
690 476 127
BbvCI CC'TCA_GC
Cuts at: 0 643 1222 1293
Size: 643 579 71
Fragments arranged by size:
643 579 71
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 901 935 1293
Size: 901 34 358
Fragments arranged by size:
901 358 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1201 1293
Size: 1201 92
BglII A'GATC_T
Cuts at: 0 769 1293
Size: 769 524
BlpI GC'TnA_GC
Cuts at: 0 400 1293
Size: 400 893
Bme1580I G_kGCm'C
Cuts at: 0 79 1293
Size: 79 1214
BmgBI CAC'GTC
Cuts at: 0 207 1293
Size: 207 1086
BmrI ACTGGGnnnn_n'
Cuts at: 0 177 1293
Size: 177 1116
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1105 1293
Size: 1105 188
Bpu10I CC'TnA_GC
Cuts at: 0 405 643 1040 1222 1293
Size: 405 238 397 182 71
Fragments arranged by size:
405 397 238 182 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1183 1293
Size: 1183 110
BsaI GGTCTCn'nnnn_
Cuts at: 0 410 1293
Size: 410 883
BsaAI yAC'GTr
Cuts at: 0 900 1293
Size: 900 393
BsaWI w'CCGG_w
Cuts at: 0 740 1293
Size: 740 553
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 857 887 1111 1141 1293
Size: 857 30 224 30 152
Fragments arranged by size:
857 224 152 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 466 631 1291 1293
Size: 466 165 660 2
Fragments arranged by size:
660 466 165 2
BseYI C'CCAG_C
Cuts at: 0 68 72 242 726 1293
Size: 68 4 170 484 567
Fragments arranged by size:
567 484 170 68 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 289 367 703 1293
Size: 220 69 78 336 590
Fragments arranged by size:
590 336 220 78 69
BsiHKAI G_wGCw'C
Cuts at: 0 740 1293
Size: 740 553
BsmBI CGTCTCn'nnnn_
Cuts at: 0 555 813 1293
Size: 555 258 480
Fragments arranged by size:
555 480 258
Bsp1286I G_dGCh'C
Cuts at: 0 79 302 740 1091 1293
Size: 79 223 438 351 202
Fragments arranged by size:
438 351 223 202 79
BspEI T'CCGG_A
Cuts at: 0 740 1293
Size: 740 553
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1040 1293
Size: 1040 253
BsrDI GCAATG_nn'
Cuts at: 0 549 1293
Size: 549 744
BssSI C'ACGA_G
Cuts at: 0 99 370 1293
Size: 99 271 923
Fragments arranged by size:
923 271 99
BstXI CCAn_nnnn'nTGG
Cuts at: 0 295 1293
Size: 295 998
BstYI r'GATC_y
Cuts at: 0 192 345 769 1293
Size: 192 153 424 524
Fragments arranged by size:
524 424 192 153
BtgI C'CryG_G
Cuts at: 0 23 1131 1148 1160 1205 1293
Size: 23 1108 17 12 45 88
Fragments arranged by size:
1108 88 45 23 17 12
BtsI GCAGTG_nn'
Cuts at: 0 1272 1293
Size: 1272 21
EaeI y'GGCC_r
Cuts at: 0 273 1293
Size: 273 1020
EarI CTCTTCn'nnn_
Cuts at: 0 586 1293
Size: 586 707
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 543 691 746 1293
Size: 543 148 55 547
Fragments arranged by size:
547 543 148 55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1105 1293
Size: 1105 188
EcoO109I rG'GnC_Cy
Cuts at: 0 375 456 514 1194 1293
Size: 375 81 58 680 99
Fragments arranged by size:
680 375 99 81 58
HaeII r_GCGC'y
Cuts at: 0 107 1293
Size: 107 1186
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 184 216 857 889 1293
Size: 184 32 641 32 404
Fragments arranged by size:
641 404 184 32 32
HincII GTy'rAC
Cuts at: 0 210 1293
Size: 210 1083
MluI A'CGCG_T
Cuts at: 0 716 1293
Size: 716 577
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 1293
Size: 560 733
MslI CAynn'nnrTG
Cuts at: 0 705 1293
Size: 705 588
MspA1I CmG'CkG
Cuts at: 0 72 114 333 687 1150 1162 1293
Size: 72 42 219 354 463 12 131
Fragments arranged by size:
463 354 219 131 72 42 12
NcoI C'CATG_G
Cuts at: 0 23 1205 1293
Size: 23 1182 88
Fragments arranged by size:
1182 88 23
NspI r_CATG'y
Cuts at: 0 466 993 1293
Size: 466 527 300
Fragments arranged by size:
527 466 300
PasI CC'CwG_GG
Cuts at: 0 1056 1293
Size: 1056 237
PfoI T'CCnGG_A
Cuts at: 0 1082 1293
Size: 1082 211
PpuMI rG'GwC_Cy
Cuts at: 0 375 456 1293
Size: 375 81 837
Fragments arranged by size:
837 375 81
PstI C_TGCA'G
Cuts at: 0 816 1178 1293
Size: 816 362 115
Fragments arranged by size:
816 362 115
PvuII CAG'CTG
Cuts at: 0 72 333 1293
Size: 72 261 960
Fragments arranged by size:
960 261 72
SacII CC_GC'GG
Cuts at: 0 1151 1163 1293
Size: 1151 12 130
Fragments arranged by size:
1151 130 12
SbfI CC_TGCA'GG
Cuts at: 0 816 1293
Size: 816 477
SfcI C'TryA_G
Cuts at: 0 80 219 360 812 1174 1293
Size: 80 139 141 452 362 119
Fragments arranged by size:
452 362 141 139 119 80
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1201 1293
Size: 1201 92
SmaI CCC'GGG
Cuts at: 0 476 1293
Size: 476 817
SmlI C'TyrA_G
Cuts at: 0 1198 1293
Size: 1198 95
SpeI A'CTAG_T
Cuts at: 0 855 1293
Size: 855 438
StyI C'CwwG_G
Cuts at: 0 23 1205 1293
Size: 23 1182 88
Fragments arranged by size:
1182 88 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 275 1061 1293
Size: 275 786 232
Fragments arranged by size:
786 275 232
TatI w'GTAC_w
Cuts at: 0 659 1293
Size: 659 634
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1080 1293
Size: 1080 213
XmaI C'CCGG_G
Cuts at: 0 474 1293
Size: 474 819
Enzymes that do cut:
AclI AflIII AleI AlwNI ApoI AvaI BamHI BanI
BanII BbsI BbvCI BcgI BglI BglII BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI
BspMI BsrDI BssSI BstXI BstYI BtgI BtsI EaeI
EarI EciI Eco57MI EcoO109I HaeII Hin4I HincII MluI
MmeI MslI MspA1I NcoI NspI PasI PfoI PpuMI
PstI PvuII SacII SbfI SfcI SfiI SmaI SmlI
SpeI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BciVI BclI BfrBI BmtI BplI
BsaBI BsaHI BsiEI BsiWI BsmI BspHI BsrBI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SalI SanDI SapI ScaI SexAI SfoI
SgfI SgrAI SnaBI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI XhoI XmnI ZraI