Restriction map for
pFN21AE6248
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# NO CUT ENZYMES ( 183 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaI HpaII KasI
KpnI KspI MaeII MamI MfeI
MluI MroI MslI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI TfiI Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AfaI 35 GTAC GT^AC
AluI 396 AGCT AG^CT
AseI 72 ATTAAT AT^TAAT
AsnI 72 ATTAAT AT^TAAT
Asp700I 508 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 369 GAGCAC G[AT]GC[AT]^C
BalI 2 TGGCCA TGG^CCA
BbsI 426 GTCTTC GAAGAC(2/6)
BmyI 369 GAGCAC G[AGT]GC[ACT]^C
BpuAI 426 GTCTTC GAAGAC(2/6)
BsaI 110 GAGACC GGTCTC(1/5)
BsaJI 185 CCTGGG C^C[ACGT][ACGT]GG
BsiHKAI 369 GAGCAC G[AT]GC[AT]^C
Bsp1286I 369 GAGCAC G[AGT]GC[ACT]^C
BspMI 37 ACCTGC ACCTGC(4/8)
Cac8I 501 GCAAGC GC[ACGT]^[ACGT]GC
DraI 25 TTTAAA TTT^AAA
EaeI 2 TGGCCA [CT]^GGCC[GA]
EcoT22I 470 ATGCAT ATGCA^T
HaeIII 3 GGCC GG^CC
MaeIII 440 GTGAC ^GT[ACGT]AC
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
NlaIV 270 GGAACC GG[ACGT]^[ACGT]CC
NsiI 470 ATGCAT ATGCA^T
PflMI 467 CCAATGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 72 ATTAAT AT^TAAT
RsaI 35 GTAC GT^AC
SfcI 131 CTACAG C^T[GA][CT]AG
SwaI 24 ATTTAAAT ATTT^AAAT
TaqI 218 TCGA T^CGA
Tsp45I 440 GTGAC ^GT[GC]AC
TspRI 207 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 218 TCGA T^CGA
Van91I 467 CCAATGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 508 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BglII 82 AGATCT A^GATCT
405 AGATCT
BsiYI 11 CCCAGTTTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
467 CCAATGCATGG
BslI 11 CCCAGTTTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
467 CCAATGCATGG
BsmAI 110 GAGAC GTCTC(1/5)
519 GAGAC
BstF5I 357 CATCC GGATG(2/0)
189 GGATG
BstYI 82 AGATCT [GA]^GATC[CT]
405 AGATCT
EarI 172 GAAGAG CTCTTC(1/4)
333 GAAGAG
FokI 357 CATCC GGATG(9/13)
189 GGATG
HphI 439 GGTGA GGTGA(8/7)
8 TCACC
Ksp632I 172 GAAGAG CTCTTC(1/4)
333 GAAGAG
MflI 82 AGATCT [GA]^GATC[CT]
405 AGATCT
MnlI 92 GAGG CCTC(7/6)
224 GAGG
MwoI 80 GCAGATCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
496 GCCTGGCAAGC
NlaIII 291 CATG CATG^
473 CATG
XhoII 82 AGATCT [GA]^GATC[CT]
405 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AcsI ApoI BbvI BfaI BsrI
BssKI BstNI DdeI DpnII EcoRII
Fnu4HI ItaI MaeI MboI MboII
MseI MvaI NdeII Sau3AI ScrFI
SfaNI Tru9I TseI Tsp509I