(Circular) (Six-base) MAPSORT of: pFN21AE1573.seq Check: 6421 from: 1 to: 7585 pFN21AE1573 7585 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4759 4759 Size: 7585 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5395 278 Size: 53 83 186 936 3859 2468 Fragments arranged by size: 3859 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2088 3330 4928 1937 Size: 151 1242 1598 4594 Fragments arranged by size: 4594 1598 1242 151 Acc65I G'GTAC_C Cuts at: 4906 4906 Size: 7585 AclI AA'CG_TT Cuts at: 1563 5712 6085 1563 Size: 4149 373 3063 Fragments arranged by size: 4149 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3276 3788 3986 4338 5648 7032 7300 1632 Size: 1644 512 198 352 1310 1384 268 1917 Fragments arranged by size: 1917 1644 1384 1310 512 352 268 198 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7388 AflIII A'CryG_T Cuts at: 1203 2781 3110 4513 6484 7461 1203 Size: 1578 329 1403 1971 977 1327 Fragments arranged by size: 1971 1578 1403 1327 977 329 AgeI A'CCGG_T Cuts at: 6416 6416 Size: 7585 AhdI GACnn_n'nnGTC Cuts at: 6314 6314 Size: 7585 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7585 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7585 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4656 4688 4656 Size: 32 7553 AlwNI CAG_nnn'CTG Cuts at: 4538 4775 5328 6900 4538 Size: 237 553 1572 5223 Fragments arranged by size: 5223 1572 553 237 ApaLI G'TGCA_C Cuts at: 5642 6798 5642 Size: 1156 6429 ApoI r'AATT_y Cuts at: 1493 2612 3456 3587 4200 4894 5120 5272 7381 Size: 1119 844 131 613 694 226 152 2109 Cuts at: 7381 1493 Size: 1697 Fragments arranged by size: 2109 1697 1119 844 694 613 226 152 131 AseI AT'TA_AT Cuts at: 160 1032 3084 6139 160 Size: 872 2052 3055 1606 Fragments arranged by size: 3055 2052 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7585 AvaI C'yCGr_G Cuts at: 1870 1943 3381 4014 4910 1870 Size: 73 1438 633 896 4545 Fragments arranged by size: 4545 1438 896 633 73 BamHI G'GATC_C Cuts at: 4829 4915 5282 4829 Size: 86 367 7132 Fragments arranged by size: 7132 367 86 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2713 4906 6361 618 Size: 325 217 627 926 2193 1455 1842 Fragments arranged by size: 2193 1842 1455 926 627 325 217 BanII G_rGCy'C Cuts at: 1391 4904 1391 Size: 3513 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2315 928 Size: 971 416 6198 Fragments arranged by size: 6198 971 416 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5776 5810 1903 Size: 34 3839 34 3678 Fragments arranged by size: 3839 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5477 6687 5477 Size: 1210 6375 BclI T'GATC_A Cuts at: 1548 2119 2142 4399 1548 Size: 571 23 2257 4734 Fragments arranged by size: 4734 2257 571 23 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2109 6196 136 Size: 107 122 71 631 605 437 4087 1525 Fragments arranged by size: 4087 1525 631 605 437 122 107 71 BglII A'GATC_T Cuts at: 3013 4046 4733 7580 3013 Size: 1033 687 2847 3018 Fragments arranged by size: 3018 2847 1033 687 BlpI GC'TnA_GC Cuts at: 5002 5002 Size: 7585 Bme1580I G_kGCm'C Cuts at: 1790 5646 6802 1790 Size: 3856 1156 2573 Fragments arranged by size: 3856 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3418 1318 Size: 2100 5485 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2072 2117 5316 Size: 416 53 83 399 24 655 45 3199 Cuts at: 5316 6274 442 Size: 958 1753 Fragments arranged by size: 3199 1753 958 655 416 399 83 53 45 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7585 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4027 4029 4027 Size: 2 7583 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2364 2866 3938 6245 1239 Size: 1125 502 1072 2307 2579 Fragments arranged by size: 2579 2307 1125 1072 502 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3288 5710 6575 6873 3288 Size: 2422 865 298 4000 Fragments arranged by size: 4000 2422 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4000 6248 882 Size: 617 2501 2248 2219 Fragments arranged by size: 2501 2248 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3068 4209 5376 493 Size: 711 1864 1141 1167 2702 Fragments arranged by size: 2702 1864 1167 1141 711 BsaBI GATnn'nnATC Cuts at: 7579 7579 Size: 7585 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5392 5774 6409 275 Size: 53 83 186 936 3859 382 635 1451 Fragments arranged by size: 3859 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3039 6018 6416 6690 6837 7248 3039 Size: 2979 398 274 147 411 3376 Fragments arranged by size: 3376 2979 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2204 2234 2362 2392 2949 Size: 30 165 30 801 30 128 30 557 Cuts at: 2949 2979 1178 Size: 30 5784 Fragments arranged by size: 5784 801 557 165 128 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2840 3376 1186 Size: 1654 536 5395 Fragments arranged by size: 5395 1654 536 BseYI C'CCAG_C Cuts at: 4833 4837 6788 7284 4833 Size: 4 1951 496 5134 Fragments arranged by size: 5134 1951 496 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4788 1163 Size: 573 3052 3960 Fragments arranged by size: 3960 3052 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5035 5796 6824 1540 Size: 275 186 3034 761 1028 2301 Fragments arranged by size: 3034 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5646 5731 6802 5646 Size: 85 1071 6429 Fragments arranged by size: 6429 1071 85 BsmI GAATG_Cn' Cuts at: 5096 5189 5096 Size: 93 7492 BsmBI CGTCTCn'nnnn_ Cuts at: 2857 4427 2857 Size: 1570 6015 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4904 5646 5731 6802 1391 Size: 399 3114 742 85 1071 2174 Fragments arranged by size: 3114 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 7248 7248 Size: 7585 BspHI T'CATG_A Cuts at: 3282 4116 5474 7204 3282 Size: 834 1358 1730 3663 Fragments arranged by size: 3663 1730 1358 834 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4371 4759 4940 844 Size: 3527 388 181 3489 Fragments arranged by size: 3527 3489 388 181 BsrBI CCG'CTC Cuts at: 1394 1823 5472 1394 Size: 429 3649 3507 Fragments arranged by size: 3649 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4372 6080 6254 65 Size: 1183 3124 1708 174 1396 Fragments arranged by size: 3124 1708 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6229 6416 1700 Size: 110 4419 187 2869 Fragments arranged by size: 4419 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2605 2798 96 Size: 2509 193 4883 Fragments arranged by size: 4883 2509 193 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7585 BssSI C'ACGA_G Cuts at: 5645 6657 5645 Size: 1012 6573 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7585 BstBI TT'CG_AA Cuts at: 6389 6389 Size: 7585 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7585 BstXI CCAn_nnnn'nTGG Cuts at: 3143 3916 4763 3143 Size: 773 847 5965 Fragments arranged by size: 5965 847 773 BstYI r'GATC_y Cuts at: 1972 2578 3013 4046 4733 4829 4915 5282 5667 Size: 606 435 1033 687 96 86 367 385 Cuts at: 5667 5684 7136 7222 7234 7580 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1033 687 606 435 385 367 346 96 86 86 17 12 BstZ17I GTA'TAC Cuts at: 2089 2089 Size: 7585 Bsu36I CC'TnA_GG Cuts at: 2175 2175 Size: 7585 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7033 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6846 Fragments arranged by size: 6846 683 56 BtsI GCAGTG_nn' Cuts at: 5101 5895 5915 5101 Size: 794 20 6771 Fragments arranged by size: 6771 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7585 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7550 DraI TTT'AAA Cuts at: 2230 3571 4698 4889 2230 Size: 1341 1127 191 4926 Fragments arranged by size: 4926 1341 1127 191 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6592 809 Size: 507 5276 1802 Fragments arranged by size: 5276 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4105 5032 5921 Size: 54 1425 50 275 18 2275 927 889 Cuts at: 5921 7550 8 Size: 1629 43 Fragments arranged by size: 2275 1629 1425 927 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5032 1537 Size: 275 3220 4090 Fragments arranged by size: 4090 3220 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2622 2703 4359 5515 1353 Size: 1269 81 1656 1156 3423 Fragments arranged by size: 3423 1656 1269 1156 81 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6172 6546 6692 387 Size: 1064 138 4583 374 146 1280 Fragments arranged by size: 4583 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2364 2866 3276 3788 3938 3986 4338 Size: 393 732 502 410 512 150 48 352 Cuts at: 4338 5648 6245 7032 7300 1239 Size: 1310 597 787 268 1524 Fragments arranged by size: 1524 1310 787 732 597 512 502 410 393 352 268 150 48 EcoO109I rG'GnC_Cy Cuts at: 2055 4841 2055 Size: 2786 4799 EcoRI G'AATT_C Cuts at: 2612 4894 5272 2612 Size: 2282 378 4925 Fragments arranged by size: 4925 2282 378 EcoRV GAT'ATC Cuts at: 3466 4850 3466 Size: 1384 6201 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2284 3220 4293 2284 Size: 936 1073 5576 Fragments arranged by size: 5576 1073 936 FspI TGC'GCA Cuts at: 6091 6091 Size: 7585 HaeII r_GCGC'y Cuts at: 1678 4144 6732 1678 Size: 2466 2588 2531 Fragments arranged by size: 2588 2531 2466 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2362 2394 2949 2981 6226 Size: 32 135 32 790 32 555 32 3245 Cuts at: 6226 6258 6300 6332 1373 Size: 32 42 32 2626 Fragments arranged by size: 3245 2626 790 555 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3183 4929 5175 669 Size: 988 281 1245 1746 246 3079 Fragments arranged by size: 3079 1746 1245 988 281 246 HindIII A'AGCT_T Cuts at: 748 3573 748 Size: 2825 4760 HpaI GTT'AAC Cuts at: 3183 5175 3183 Size: 1992 5593 KpnI G_GTAC'C Cuts at: 4910 4910 Size: 7585 MfeI C'AATT_G Cuts at: 5184 5184 Size: 7585 MluI A'CGCG_T Cuts at: 7461 7461 Size: 7585 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3373 4125 4450 6699 6883 1305 Size: 2068 752 325 2249 184 2007 Fragments arranged by size: 2249 2068 2007 752 325 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5763 Fragments arranged by size: 5763 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2138 5543 5902 6061 518 Size: 648 972 3405 359 159 2042 Fragments arranged by size: 3405 2042 972 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3916 4837 5001 5678 6826 7071 1606 Size: 2310 921 164 677 1148 245 2120 Fragments arranged by size: 2310 2120 1148 921 677 245 164 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7033 NdeI CA'TA_TG Cuts at: 387 2335 387 Size: 1948 5637 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7585 NotI GC'GGCC_GC Cuts at: 5032 5032 Size: 7585 NspI r_CATG'y Cuts at: 1132 1229 2785 3114 3625 4489 4517 4582 4943 Size: 97 1556 329 511 864 28 65 361 Cuts at: 4943 6488 1132 Size: 1545 2229 Fragments arranged by size: 2229 1556 1545 864 511 361 329 97 65 28 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7585 PciI A'CATG_T Cuts at: 2781 3110 4513 6484 2781 Size: 329 1403 1971 3882 Fragments arranged by size: 3882 1971 1403 329 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7585 PmeI GTTT'AAAC Cuts at: 4889 4889 Size: 7585 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5654 5686 7167 7199 5654 Size: 32 1481 32 6040 Fragments arranged by size: 6040 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2055 4841 2055 Size: 2786 4799 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7585 PsiI TTA'TAA Cuts at: 4647 5155 4647 Size: 508 7077 PspXI vC'TCGA_Gb Cuts at: 3381 3381 Size: 7585 PstI C_TGCA'G Cuts at: 830 4450 4937 830 Size: 3620 487 3478 Fragments arranged by size: 3620 3478 487 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7585 PvuII CAG'CTG Cuts at: 3916 4837 3916 Size: 921 6664 SalI G'TCGA_C Cuts at: 1936 4927 1936 Size: 2991 4594 SanDI GG'GwC_CC Cuts at: 2055 2055 Size: 7585 SbfI CC_TGCA'GG Cuts at: 4937 4937 Size: 7585 ScaI AGT'ACT Cuts at: 5833 7545 5833 Size: 1712 5873 SfcI C'TryA_G Cuts at: 826 1045 2092 2182 3327 4167 4446 4933 6068 Size: 219 1047 90 1145 840 279 487 1135 Cuts at: 6068 6749 6940 826 Size: 681 191 1471 Fragments arranged by size: 1471 1145 1135 1047 840 681 487 279 219 191 90 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7585 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7585 SmaI CCC'GGG Cuts at: 1872 4912 1872 Size: 3040 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3303 3381 5689 6590 6852 820 Size: 197 926 1360 78 2308 901 262 1553 Fragments arranged by size: 2308 1553 1360 926 901 262 197 78 SnaBI TAC'GTA Cuts at: 493 4209 5376 493 Size: 3716 1167 2702 Fragments arranged by size: 3716 2702 1167 SpeI A'CTAG_T Cuts at: 152 152 Size: 7585 SphI G_CATG'C Cuts at: 1132 4943 1132 Size: 3811 3774 SspI AAT'ATT Cuts at: 5 52 3082 5509 5 Size: 47 3030 2427 2081 Fragments arranged by size: 3030 2427 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7585 StyI C'CwwG_G Cuts at: 513 1065 2289 2943 3006 3249 5024 513 Size: 552 1224 654 63 243 1775 3074 Fragments arranged by size: 3074 1775 1224 654 552 243 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2270 4799 5612 5629 5782 5967 6412 Size: 1255 690 2529 813 17 153 185 445 Cuts at: 6412 325 Size: 1498 Fragments arranged by size: 2529 1498 1255 813 690 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2605 2798 4308 Size: 274 80 33 51 1443 628 193 1510 Cuts at: 4308 4575 5831 7543 96 Size: 267 1256 1712 138 Fragments arranged by size: 1712 1510 1443 1256 628 274 267 193 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2480 2512 7545 7577 2480 Size: 32 5033 32 2488 Fragments arranged by size: 5033 2488 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2963 1316 Size: 1647 5938 XbaI T'CTAG_A Cuts at: 4921 4921 Size: 7585 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4841 4841 Size: 7585 XhoI C'TCGA_G Cuts at: 1943 3381 1943 Size: 1438 6147 XmaI C'CCGG_G Cuts at: 1870 4910 1870 Size: 3040 4545 XmnI GAAnn'nnTTC Cuts at: 4735 5714 4735 Size: 979 6606 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5393 276 Size: 53 83 186 936 3859 2468 Fragments arranged by size: 3859 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI