(Linear) (Six-base) MAPSORT of: KIEE1573.seq Check: 3561 from: 1 to: 2880 KIEE1573.orf With 182 enzymes: SgfI * July 15, 2010 18:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2754 2880 Size: 2754 126 AccI GT'mk_AC Cuts at: 0 83 1325 2880 Size: 83 1242 1555 Fragments arranged by size: 1555 1242 83 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1271 1783 1981 2333 2880 Size: 1271 512 198 352 547 Fragments arranged by size: 1271 547 512 352 198 AflIII A'CryG_T Cuts at: 0 776 1105 2508 2880 Size: 776 329 1403 372 Fragments arranged by size: 1403 776 372 329 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2651 2683 2880 Size: 2651 32 197 Fragments arranged by size: 2651 197 32 AlwNI CAG_nnn'CTG Cuts at: 0 2533 2770 2880 Size: 2533 237 110 Fragments arranged by size: 2533 237 110 ApoI r'AATT_y Cuts at: 0 607 1451 1582 2195 2880 Size: 607 844 131 613 685 Fragments arranged by size: 844 685 613 607 131 AseI AT'TA_AT Cuts at: 0 1079 2880 Size: 1079 1801 AvaI C'yCGr_G Cuts at: 0 1376 2009 2880 Size: 1376 633 871 Fragments arranged by size: 1376 871 633 BamHI G'GATC_C Cuts at: 0 2824 2880 Size: 2824 56 BanI G'GyrC_C Cuts at: 0 708 2880 Size: 708 2172 BbsI GAAGACnn'nnnn_ Cuts at: 0 310 2880 Size: 310 2570 BclI T'GATC_A Cuts at: 0 114 137 2394 2880 Size: 114 23 2257 486 Fragments arranged by size: 2257 486 114 23 BglI GCCn_nnn'nGGC Cuts at: 0 104 2880 Size: 104 2776 BglII A'GATC_T Cuts at: 0 1008 2041 2728 2880 Size: 1008 1033 687 152 Fragments arranged by size: 1033 1008 687 152 BmgBI CAC'GTC Cuts at: 0 1413 2880 Size: 1413 1467 BmrI ACTGGGnnnn_n' Cuts at: 0 67 112 2880 Size: 67 45 2768 Fragments arranged by size: 2768 67 45 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2022 2024 2880 Size: 2022 2 856 Fragments arranged by size: 2022 856 2 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 359 861 1933 2880 Size: 359 502 1072 947 Fragments arranged by size: 1072 947 502 359 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1283 2880 Size: 1283 1597 BsaI GGTCTCn'nnnn_ Cuts at: 0 1995 2880 Size: 1995 885 BsaAI yAC'GTr Cuts at: 0 1063 2204 2880 Size: 1063 1141 676 Fragments arranged by size: 1141 1063 676 BsaWI w'CCGG_w Cuts at: 0 1034 2880 Size: 1034 1846 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 199 229 357 387 944 974 2880 Size: 199 30 128 30 557 30 1906 Fragments arranged by size: 1906 557 199 128 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 835 1371 2880 Size: 835 536 1509 Fragments arranged by size: 1509 835 536 BseYI C'CCAG_C Cuts at: 0 2828 2832 2880 Size: 2828 4 48 Fragments arranged by size: 2828 48 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2783 2880 Size: 2783 97 BsmBI CGTCTCn'nnnn_ Cuts at: 0 852 2422 2880 Size: 852 1570 458 Fragments arranged by size: 1570 852 458 BspHI T'CATG_A Cuts at: 0 1277 2111 2880 Size: 1277 834 769 Fragments arranged by size: 1277 834 769 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2366 2754 2880 Size: 2366 388 126 Fragments arranged by size: 2366 388 126 BsrDI GCAATG_nn' Cuts at: 0 2367 2880 Size: 2367 513 BsrGI T'GTAC_A Cuts at: 0 600 793 2880 Size: 600 193 2087 Fragments arranged by size: 2087 600 193 BstXI CCAn_nnnn'nTGG Cuts at: 0 1138 1911 2758 2880 Size: 1138 773 847 122 Fragments arranged by size: 1138 847 773 122 BstYI r'GATC_y Cuts at: 0 573 1008 2041 2728 2824 2880 Size: 573 435 1033 687 96 56 Fragments arranged by size: 1033 687 573 435 96 56 BstZ17I GTA'TAC Cuts at: 0 84 2880 Size: 84 2796 Bsu36I CC'TnA_GG Cuts at: 0 170 2880 Size: 170 2710 DraI TTT'AAA Cuts at: 0 225 1566 2693 2880 Size: 225 1341 1127 187 Fragments arranged by size: 1341 1127 225 187 EaeI y'GGCC_r Cuts at: 0 2100 2880 Size: 2100 780 EarI CTCTTCn'nnn_ Cuts at: 0 617 698 2354 2880 Size: 617 81 1656 526 Fragments arranged by size: 1656 617 526 81 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 359 861 1271 1783 1933 1981 2333 2880 Size: 359 502 410 512 150 48 352 547 Fragments arranged by size: 547 512 502 410 359 352 150 48 EcoO109I rG'GnC_Cy Cuts at: 0 50 2836 2880 Size: 50 2786 44 Fragments arranged by size: 2786 50 44 EcoRI G'AATT_C Cuts at: 0 607 2880 Size: 607 2273 EcoRV GAT'ATC Cuts at: 0 1461 2845 2880 Size: 1461 1384 35 Fragments arranged by size: 1461 1384 35 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 279 1215 2288 2880 Size: 279 936 1073 592 Fragments arranged by size: 1073 936 592 279 HaeII r_GCGC'y Cuts at: 0 2139 2880 Size: 2139 741 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 357 389 944 976 2880 Size: 357 32 555 32 1904 Fragments arranged by size: 1904 555 357 32 32 HincII GTy'rAC Cuts at: 0 1178 2880 Size: 1178 1702 HindIII A'AGCT_T Cuts at: 0 1568 2880 Size: 1568 1312 HpaI GTT'AAC Cuts at: 0 1178 2880 Size: 1178 1702 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1368 2120 2445 2880 Size: 1368 752 325 435 Fragments arranged by size: 1368 752 435 325 MslI CAynn'nnrTG Cuts at: 0 133 2880 Size: 133 2747 MspA1I CmG'CkG Cuts at: 0 1911 2832 2880 Size: 1911 921 48 Fragments arranged by size: 1911 921 48 NdeI CA'TA_TG Cuts at: 0 330 2880 Size: 330 2550 NspI r_CATG'y Cuts at: 0 780 1109 1620 2484 2512 2577 2880 Size: 780 329 511 864 28 65 303 Fragments arranged by size: 864 780 511 329 303 65 28 PciI A'CATG_T Cuts at: 0 776 1105 2508 2880 Size: 776 329 1403 372 Fragments arranged by size: 1403 776 372 329 PpuMI rG'GwC_Cy Cuts at: 0 50 2836 2880 Size: 50 2786 44 Fragments arranged by size: 2786 50 44 PsiI TTA'TAA Cuts at: 0 2642 2880 Size: 2642 238 PspXI vC'TCGA_Gb Cuts at: 0 1376 2880 Size: 1376 1504 PstI C_TGCA'G Cuts at: 0 2445 2880 Size: 2445 435 PvuII CAG'CTG Cuts at: 0 1911 2832 2880 Size: 1911 921 48 Fragments arranged by size: 1911 921 48 SanDI GG'GwC_CC Cuts at: 0 50 2880 Size: 50 2830 SfcI C'TryA_G Cuts at: 0 87 177 1322 2162 2441 2880 Size: 87 90 1145 840 279 439 Fragments arranged by size: 1145 840 439 279 90 87 SmlI C'TyrA_G Cuts at: 0 1298 1376 2880 Size: 1298 78 1504 Fragments arranged by size: 1504 1298 78 SnaBI TAC'GTA Cuts at: 0 2204 2880 Size: 2204 676 SspI AAT'ATT Cuts at: 0 1077 2880 Size: 1077 1803 StyI C'CwwG_G Cuts at: 0 284 938 1001 1244 2880 Size: 284 654 63 243 1636 Fragments arranged by size: 1636 654 284 243 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 265 2794 2880 Size: 265 2529 86 Fragments arranged by size: 2529 265 86 TatI w'GTAC_w Cuts at: 0 600 793 2303 2570 2880 Size: 600 193 1510 267 310 Fragments arranged by size: 1510 600 310 267 193 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 475 507 2880 Size: 475 32 2373 Fragments arranged by size: 2373 475 32 Tth111I GACn'n_nGTC Cuts at: 0 958 2880 Size: 958 1922 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2836 2880 Size: 2836 44 XhoI C'TCGA_G Cuts at: 0 1376 2880 Size: 1376 1504 XmnI GAAnn'nnTTC Cuts at: 0 2730 2880 Size: 2730 150 Enzymes that do cut: AarI AccI AcuI AflIII AloI AlwNI ApoI AseI AvaI BamHI BanI BbsI BclI BglI BglII BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsmBI BspHI BspMI BsrDI BsrGI BstXI BstYI BstZ17I Bsu36I DraI EaeI EarI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII HpaI MmeI MslI MspA1I NdeI NspI PciI PpuMI PsiI PspXI PstI PvuII SanDI SfcI SmlI SnaBI SspI StyI TaqII TatI TstI Tth111I XcmI XhoI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI ApaI ApaLI AscI AsiSI AvrII BaeI BanII BbeI BbvCI BcgI BciVI BfrBI BlpI Bme1580I BmtI Bpu10I BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoNI FseI FspI FspAI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PsrI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SphI SrfI StuI SwaI XbaI XmaI ZraI