Restriction map for
pFN21AE6702
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# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccII AcsI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI ApoI AscI Asp718I
AspEI AspI AviII BamHI BbrPI
BcgI BfrI BsaAI BsaBI BsaI
BsiWI BsmI BspDI BspHI BspLU11I
BsrFI BssHII Bst1107I BstBI BstUI
BstZ17I Cfr10I ClaI CpoI DraI
DrdI Eam1105I Eco47III EcoRI EcoRV
FseI FspI HpaI KpnI KspI
MamI MfeI MluI MunI MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PvuI RcaI RsrII SacII
SapI SfuI SgrAI SnaBI SpeI
SwaI Tsp509I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccI 1705 GTCGAC GT^[AC][GT]AC
AccIII 1517 TCCGGA T^CCGGA
AclI 1870 AACGTT AA^CGTT
AcyI 1816 GGCGCC G[GA]^CG[CT]C
AocI 1101 CCTGAGG CC^T[ACGT]AGG
AseI 1831 ATTAAT AT^TAAT
AsnI 1831 ATTAAT AT^TAAT
AvrII 151 CCTAGG C^CTAGG
BalI 299 TGGCCA TGG^CCA
BbeI 1816 GGCGCC GGCGC^C
BciVI 249 GTATCC GTATCC(6/5)
BclI 509 TGATCA T^GATCA
BfaI 152 CTAG C^TAG
BglII 1091 AGATCT A^GATCT
BlnI 151 CCTAGG C^CTAGG
BsaHI 1816 GGCGCC G[GA]^CG[CT]C
BsaWI 1517 TCCGGA [AT]^CCGG[AT]
BseAI 1517 TCCGGA T^CCGGA
BsgI 670 GTGCAG GTGCAG(16/14)
BsiEI 97 CGGCCG CG[GA][CT]^CG
BsmBI 681 CGTCTC CGTCTC(1/5)
Bsp1407I 2105 TGTACA T^GTACA
BspEI 1517 TCCGGA T^CCGGA
BsrBI 1629 CCGCTC CCGCTC(-3/-3)
BsrGI 2105 TGTACA T^GTACA
BstAPI 864 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1101 CCTGAGG CC^T[ACGT]AGG
EagI 97 CGGCCG C^GGCCG
EclXI 97 CGGCCG C^GGCCG
Eco52I 97 CGGCCG C^GGCCG
Eco81I 1101 CCTGAGG CC^T[ACGT]AGG
EcoT22I 2165 ATGCAT ATGCA^T
FauI 486 CCCGC CCCGC(4/6)
FbaI 509 TGATCA T^GATCA
HgaI 16 GCGTC GACGC(5/10)
Hin1I 1816 GGCGCC G[GA]^CG[CT]C
HindIII 1786 AAGCTT A^AGCTT
KasI 1816 GGCGCC G^GCGCC
MaeI 152 CTAG C^TAG
MaeII 1871 ACGT A^CGT
MluNI 299 TGGCCA TGG^CCA
MroI 1517 TCCGGA T^CCGGA
MscI 299 TGGCCA TGG^CCA
MseI 1832 TTAA T^TAA
NarI 1816 GGCGCC GG^CGCC
NsiI 2165 ATGCAT ATGCA^T
PflMI 1131 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1831 ATTAAT AT^TAAT
Psp1406I 1870 AACGTT AA^CGTT
SacI 1896 GAGCTC GAGCT^C
SalI 1705 GTCGAC G^TCGAC
SbfI 979 CCTGCAGG CCTGCA^GG
ScaI 1589 AGTACT AGT^ACT
SexAI 172 ACCTGGT A^CC[AT]GGT
SfiI 483 GGCCCCGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1816 GGCGCC GGC^GCC
SphI 1364 GCATGC GCATG^C
Sse8387I 979 CCTGCAGG CCTGCA^GG
SspBI 2105 TGTACA T^GTACA
SspI 2068 AATATT AAT^ATT
TfiI 475 GAATC G^A[AT]TC
Tru9I 1832 TTAA T^TAA
Van91I 1131 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 600 GATCC GGATC(4/5)
1416 GGATC
BanII 141 GGGCTC G[GA]GC[CT]^C
1896 GAGCTC
BbvCI 1987 CCTCAGC CCTCAGC(-5/-2)
1368 GCTGAGG
BlpI 143 GCTCAGC GC^T[ACGT]AGC
1377 GCTCAGC
BpmI 757 CTGGAG CTGGAG(16/14)
1253 CTGGAG
Bpu1102I 143 GCTCAGC GC^T[ACGT]AGC
1377 GCTCAGC
BsrDI 1071 CATTGC GCAATG(2/0)
61 GCAATG
BstXI 1049 CCATCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1076 CCATCTCACTGG
BstYI 1091 AGATCT [GA]^GATC[CT]
1416 GGATCT
CelII 143 GCTCAGC GC^T[ACGT]AGC
1377 GCTCAGC
DraIII 557 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2043 CACTGTGTG
DsaI 428 CCGTGG C^C[GA][CT]GG
797 CCGTGG
HincII 1705 GTCGAC GT[CT]^[GA]AC
1837 GTCAAC
HindII 1705 GTCGAC GT[CT]^[GA]AC
1837 GTCAAC
MflI 1091 AGATCT [GA]^GATC[CT]
1416 GGATCT
NspI 1364 GCATGC [GA]CATG^[CT]
1385 ACATGC
PpuMI 154 AGGACCC [GA]G^G[AT]CC[CT]
433 GGGACCT
SmaI 536 CCCGGG CCC^GGG
886 CCCGGG
StuI 491 AGGCCT AGG^CCT
2236 AGGCCT
TaqI 1706 TCGA T^CGA
2162 TCGA
TthHB8I 1706 TCGA T^CGA
2162 TCGA
XhoII 1091 AGATCT [GA]^GATC[CT]
1416 GGATCT
XmaCI 536 CCCGGG C^CCGGG
886 CCCGGG
XmaI 536 CCCGGG C^CCGGG
886 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AfaI AluI AlwNI Asp700I
AspHI AvaI AvaII BanI BbsI
BbvI BcnI BglI BmyI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BssSI BstEII BstF5I
BstNI BstPI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TseI Tsp45I TspRI XcmI
XmnI