(Circular) (Six-base) MAPSORT of: pFN21AE6702.seq Check: 1217 from: 1 to: 6979 pFN21AE6702 6979 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3547 3547 Size: 6979 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4789 278 Size: 53 83 186 936 3253 2468 Fragments arranged by size: 3253 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3711 4322 1937 Size: 1774 611 4594 Fragments arranged by size: 4594 1774 611 Acc65I G'GTAC_C Cuts at: 4300 4300 Size: 6979 AclI AA'CG_TT Cuts at: 1563 3876 5106 5479 1563 Size: 2313 1230 373 3063 Fragments arranged by size: 3063 2313 1230 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3383 3707 3782 3788 3995 4088 5042 6426 Size: 1751 324 75 6 207 93 954 1384 Cuts at: 6426 6694 1632 Size: 268 1917 Fragments arranged by size: 1917 1751 1384 954 324 268 207 93 75 6 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6782 AflIII A'CryG_T Cuts at: 1203 5878 6855 1203 Size: 4675 977 1327 Fragments arranged by size: 4675 1327 977 AgeI A'CCGG_T Cuts at: 5810 5810 Size: 6979 AhdI GACnn_n'nnGTC Cuts at: 5708 5708 Size: 6979 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6979 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4212 1184 Size: 3028 3951 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2631 2663 2631 Size: 32 6947 AlwNI CAG_nnn'CTG Cuts at: 2047 2875 3142 4722 6294 2047 Size: 828 267 1580 1572 2732 Fragments arranged by size: 2732 1580 1572 828 267 ApaLI G'TGCA_C Cuts at: 5036 6192 5036 Size: 1156 5823 ApoI r'AATT_y Cuts at: 1493 4288 4514 4666 6775 1493 Size: 2795 226 152 2109 1697 Fragments arranged by size: 2795 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 3837 5533 160 Size: 872 2805 1696 1606 Fragments arranged by size: 2805 1696 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6979 AvaI C'yCGr_G Cuts at: 1870 1943 2199 2541 2891 4237 4304 1870 Size: 73 256 342 350 1346 67 4545 Fragments arranged by size: 4545 1346 350 342 256 73 67 AvrII C'CTAG_G Cuts at: 2156 2156 Size: 6979 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2237 2270 2237 Size: 33 6946 BamHI G'GATC_C Cuts at: 4309 4676 4309 Size: 367 6612 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2798 2995 3559 3821 4028 Size: 325 217 627 1011 197 564 262 207 Cuts at: 4028 4300 5755 618 Size: 272 1455 1842 Fragments arranged by size: 1842 1455 1011 627 564 325 272 262 217 207 197 BanII G_rGCy'C Cuts at: 1391 2150 3905 4298 1391 Size: 759 1755 393 4072 Fragments arranged by size: 4072 1755 759 393 BbeI G_GCGC'C Cuts at: 3825 3825 Size: 6979 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2386 2674 3622 3864 3951 928 Size: 971 487 288 948 242 87 3956 Fragments arranged by size: 3956 971 948 487 288 242 87 BbvCI CC'TCA_GC Cuts at: 3374 3993 3374 Size: 619 6360 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5170 5204 1903 Size: 34 3233 34 3678 Fragments arranged by size: 3678 3233 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2265 4871 6081 2265 Size: 2606 1210 3163 Fragments arranged by size: 3163 2606 1210 BclI T'GATC_A Cuts at: 1548 2514 1548 Size: 966 6013 BfrBI ATG'CAT Cuts at: 4172 4172 Size: 6979 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2345 2495 3416 Size: 107 122 71 631 605 673 150 921 Cuts at: 3416 5590 136 Size: 2174 1525 Fragments arranged by size: 2174 1525 921 673 631 605 150 122 107 71 BglII A'GATC_T Cuts at: 3096 6974 3096 Size: 3878 3101 BlpI GC'TnA_GC Cuts at: 2149 3383 4396 2149 Size: 1234 1013 4732 Fragments arranged by size: 4732 1234 1013 Bme1580I G_kGCm'C Cuts at: 1790 3000 4033 4161 5040 6196 1790 Size: 1210 1033 128 879 1156 2573 Fragments arranged by size: 2573 1210 1156 1033 879 128 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6979 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2996 3098 4710 Size: 416 53 83 399 24 1579 102 1612 Cuts at: 4710 5668 442 Size: 958 1753 Fragments arranged by size: 1753 1612 1579 958 416 399 102 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6979 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2783 3279 5639 1239 Size: 1544 496 2360 2579 Fragments arranged by size: 2579 2360 1544 496 Bpu10I CC'TnA_GC Cuts at: 3374 3904 3993 3374 Size: 530 89 6360 Fragments arranged by size: 6360 530 89 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2681 3728 4043 4169 5104 5969 6267 2681 Size: 1047 315 126 935 865 298 3393 Fragments arranged by size: 3393 1047 935 865 315 298 126 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5642 882 Size: 617 4143 2219 Fragments arranged by size: 4143 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4770 493 Size: 711 3566 2702 Fragments arranged by size: 3566 2702 711 BsaBI GATnn'nnATC Cuts at: 6973 6973 Size: 6979 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3822 4786 5168 5803 Size: 53 83 186 936 2289 964 382 635 Cuts at: 5803 275 Size: 1451 Fragments arranged by size: 2289 1451 964 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3522 5412 5810 6084 6231 6642 3522 Size: 1890 398 274 147 411 3859 Fragments arranged by size: 3859 1890 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3380 3410 1178 Size: 30 165 30 1977 30 4747 Fragments arranged by size: 4747 1977 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2144 3275 3628 3800 3880 1186 Size: 958 1131 353 172 80 4285 Fragments arranged by size: 4285 1131 958 353 172 80 BseYI C'CCAG_C Cuts at: 2134 2142 2596 2822 3018 4154 6182 6678 2134 Size: 8 454 226 196 1136 2028 496 2435 Fragments arranged by size: 2435 2028 1136 496 454 226 196 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2696 1163 Size: 573 960 5446 Fragments arranged by size: 5446 960 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2105 4429 5190 6218 1540 Size: 275 186 104 2324 761 1028 2301 Fragments arranged by size: 2324 2301 1028 761 275 186 104 BsiHKAI G_wGCw'C Cuts at: 2192 2200 2835 3236 3905 5040 5125 6196 2192 Size: 8 635 401 669 1135 85 1071 2975 Fragments arranged by size: 2975 1135 1071 669 635 401 85 8 BsmI GAATG_Cn' Cuts at: 4490 4583 4490 Size: 93 6886 BsmBI CGTCTCn'nnnn_ Cuts at: 2692 2692 Size: 6979 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2150 2192 2200 2835 3000 3236 3905 Size: 399 360 42 8 635 165 236 669 Cuts at: 3905 4033 4161 4298 5040 5125 6196 1391 Size: 128 128 137 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 669 635 399 360 236 165 137 128 128 85 42 8 BspEI T'CCGG_A Cuts at: 3522 6642 3522 Size: 3120 3859 BspHI T'CATG_A Cuts at: 4868 6598 4868 Size: 1730 5249 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2082 2668 3241 3330 3547 4334 844 Size: 1238 586 573 89 217 787 3489 Fragments arranged by size: 3489 1238 787 586 573 217 89 BsrBI CCG'CTC Cuts at: 1394 1823 3636 4866 1394 Size: 429 1813 1230 3507 Fragments arranged by size: 3507 1813 1230 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2073 3075 5474 5648 65 Size: 1183 825 1002 2399 174 1396 Fragments arranged by size: 2399 1396 1183 1002 825 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5623 5810 1700 Size: 110 3813 187 2869 Fragments arranged by size: 3813 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4110 96 Size: 4014 2965 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6979 BssSI C'ACGA_G Cuts at: 2834 3035 3512 5039 6051 2834 Size: 201 477 1527 1012 3762 Fragments arranged by size: 3762 1527 1012 477 201 BstAPI GCAn_nnn'nTGC Cuts at: 794 2875 794 Size: 2081 4898 BstBI TT'CG_AA Cuts at: 5783 5783 Size: 6979 BstEII G'GTnAC_C Cuts at: 1184 2244 2279 2623 3439 4267 1184 Size: 1060 35 344 816 828 3896 Fragments arranged by size: 3896 1060 828 816 344 35 BstXI CCAn_nnnn'nTGG Cuts at: 2011 3061 3088 2011 Size: 1050 27 5902 Fragments arranged by size: 5902 1050 27 BstYI r'GATC_y Cuts at: 1972 3096 3421 4309 4676 5061 5078 6530 6616 Size: 1124 325 888 367 385 17 1452 86 Cuts at: 6616 6628 6974 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1124 888 385 367 346 325 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3107 3107 Size: 6979 BtgI C'CryG_G Cuts at: 513 1065 2004 2433 2802 513 Size: 552 939 429 369 4690 Fragments arranged by size: 4690 939 552 429 369 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3040 487 Size: 683 56 1814 4426 Fragments arranged by size: 4426 1814 683 56 BtsI GCAGTG_nn' Cuts at: 2045 2192 2476 2808 4495 5289 5309 2045 Size: 147 284 332 1687 794 20 3715 Fragments arranged by size: 3715 1687 794 332 284 147 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6979 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3018 3053 529 Size: 35 2454 35 4455 Fragments arranged by size: 4455 2454 35 35 DraI TTT'AAA Cuts at: 4283 4283 Size: 6979 DraIII CAC_nnn'GTG Cuts at: 2567 4053 2567 Size: 1486 5493 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5986 809 Size: 507 4670 1802 Fragments arranged by size: 4670 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2102 2304 3450 Size: 54 1425 50 275 18 272 202 1146 Cuts at: 3450 3642 4426 5315 6944 8 Size: 192 784 889 1629 43 Fragments arranged by size: 1629 1425 1146 889 784 275 272 202 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2102 4426 1537 Size: 275 290 2324 4090 Fragments arranged by size: 4090 2324 290 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2588 3274 3317 4909 1353 Size: 1235 686 43 1592 3423 Fragments arranged by size: 3423 1592 1235 686 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2030 2188 3434 5566 5940 6086 Size: 1064 138 441 158 1246 2132 374 146 Cuts at: 6086 387 Size: 1280 Fragments arranged by size: 2132 1280 1246 1064 441 374 158 146 138 EcoICRI GAG'CTC Cuts at: 3903 3903 Size: 6979 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2783 3279 3383 3707 3782 3788 3995 Size: 393 1151 496 104 324 75 6 207 Cuts at: 3995 4088 5042 5639 6426 6694 1239 Size: 93 954 597 787 268 1524 Fragments arranged by size: 1524 1151 954 787 597 496 393 324 268 207 104 93 75 6 EcoNI CCTnn'n_nnAGG Cuts at: 2585 3224 3326 3404 3554 2585 Size: 639 102 78 150 6010 Fragments arranged by size: 6010 639 150 102 78 EcoO109I rG'GnC_Cy Cuts at: 2160 2439 3955 3979 2160 Size: 279 1516 24 5160 Fragments arranged by size: 5160 1516 279 24 EcoRI G'AATT_C Cuts at: 4288 4666 4288 Size: 378 6601 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4210 4210 Size: 6979 FspI TGC'GCA Cuts at: 5485 5485 Size: 6979 HaeII r_GCGC'y Cuts at: 1678 2143 3048 3825 6126 1678 Size: 465 905 777 2301 2531 Fragments arranged by size: 2531 2301 905 777 465 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2273 2305 3380 3412 5620 Size: 32 135 32 701 32 1075 32 2208 Cuts at: 5620 5652 5694 5726 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2208 1075 701 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3712 3844 4323 4569 669 Size: 988 281 1774 132 479 246 3079 Fragments arranged by size: 3079 1774 988 479 281 246 132 HindIII A'AGCT_T Cuts at: 748 3791 748 Size: 3043 3936 HpaI GTT'AAC Cuts at: 4569 4569 Size: 6979 KasI G'GCGC_C Cuts at: 3821 3821 Size: 6979 KpnI G_GTAC'C Cuts at: 4304 4304 Size: 6979 MfeI C'AATT_G Cuts at: 4578 4578 Size: 6979 MluI A'CGCG_T Cuts at: 6855 6855 Size: 6979 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2245 4255 6093 6277 1305 Size: 940 2010 1838 184 2007 Fragments arranged by size: 2010 2007 1838 940 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2306 10 Size: 54 1425 343 474 4683 Fragments arranged by size: 4683 1425 474 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2432 2456 3059 3353 4937 5296 5455 Size: 648 1266 24 603 294 1584 359 159 Cuts at: 5455 518 Size: 2042 Fragments arranged by size: 2042 1584 1266 648 603 359 294 159 24 MspA1I CmG'CkG Cuts at: 1606 2107 2666 2672 2816 2875 3022 3760 4395 Size: 501 559 6 144 59 147 738 635 Cuts at: 4395 5072 6220 6465 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 738 677 635 559 501 245 147 144 59 6 NarI GG'CG_CC Cuts at: 3822 3822 Size: 6979 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5488 Fragments arranged by size: 5488 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6979 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6979 NotI GC'GGCC_GC Cuts at: 4426 4426 Size: 6979 NsiI A_TGCA'T Cuts at: 4174 4174 Size: 6979 NspI r_CATG'y Cuts at: 1132 1229 3373 3394 4337 5882 1132 Size: 97 2144 21 943 1545 2229 Fragments arranged by size: 2229 2144 1545 943 97 21 PasI CC'CwG_GG Cuts at: 1348 3151 3982 1348 Size: 1803 831 4345 Fragments arranged by size: 4345 1803 831 PciI A'CATG_T Cuts at: 5878 5878 Size: 6979 PflMI CCAn_nnn'nTGG Cuts at: 1108 3142 1108 Size: 2034 4945 PfoI T'CCnGG_A Cuts at: 4017 4089 4017 Size: 72 6907 PmeI GTTT'AAAC Cuts at: 4283 4283 Size: 6979 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5048 5080 6561 6593 5048 Size: 32 1481 32 5434 Fragments arranged by size: 5434 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2160 2439 2160 Size: 279 6700 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6979 PsiI TTA'TAA Cuts at: 4549 4549 Size: 6979 PstI C_TGCA'G Cuts at: 830 2671 2815 2868 2989 3780 4331 830 Size: 1841 144 53 121 791 551 3478 Fragments arranged by size: 3478 1841 791 551 144 121 53 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6979 PvuII CAG'CTG Cuts at: 2666 2672 2816 2875 3022 3760 2666 Size: 6 144 59 147 738 5885 Fragments arranged by size: 5885 738 147 144 59 6 SacI G_AGCT'C Cuts at: 3905 3905 Size: 6979 SalI G'TCGA_C Cuts at: 1936 3710 4321 1936 Size: 1774 611 4594 Fragments arranged by size: 4594 1774 611 SbfI CC_TGCA'GG Cuts at: 2989 4331 2989 Size: 1342 5637 ScaI AGT'ACT Cuts at: 3596 5227 6939 3596 Size: 1631 1712 3636 Fragments arranged by size: 3636 1712 1631 SexAI A'CCwGG_T Cuts at: 2177 2177 Size: 6979 SfcI C'TryA_G Cuts at: 826 1045 2374 2667 2811 2864 2985 3673 3776 Size: 219 1329 293 144 53 121 688 103 Cuts at: 3776 4327 5462 6143 6334 826 Size: 551 1135 681 191 1471 Fragments arranged by size: 1471 1329 1135 688 681 551 293 219 191 144 121 103 53 SfiI GGCCn_nnn'nGGCC Cuts at: 2495 2495 Size: 6979 SfoI GGC'GCC Cuts at: 3823 3823 Size: 6979 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6979 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6979 SmaI CCC'GGG Cuts at: 1872 2543 2893 4306 1872 Size: 671 350 1413 4545 Fragments arranged by size: 4545 1413 671 350 SmlI C'TyrA_G Cuts at: 820 1017 1943 2696 3743 4058 4148 5083 5984 Size: 197 926 753 1047 315 90 935 901 Cuts at: 5984 6246 820 Size: 262 1553 Fragments arranged by size: 1553 1047 935 926 901 753 315 262 197 90 SnaBI TAC'GTA Cuts at: 493 4770 493 Size: 4277 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6979 SphI G_CATG'C Cuts at: 1132 3373 4337 1132 Size: 2241 964 3774 Fragments arranged by size: 3774 2241 964 SspI AAT'ATT Cuts at: 5 52 4075 4903 5 Size: 47 4023 828 2081 Fragments arranged by size: 4023 2081 828 47 StuI AGG'CCT Cuts at: 1517 2498 4243 1517 Size: 981 1745 4253 Fragments arranged by size: 4253 1745 981 StyI C'CwwG_G Cuts at: 513 1065 2004 2156 3327 3475 3606 3816 3951 Size: 552 939 152 1171 148 131 210 135 Cuts at: 3951 4418 513 Size: 467 3074 Fragments arranged by size: 3074 1171 939 552 467 210 152 148 135 131 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2044 2267 3032 4016 4255 5006 5023 Size: 1255 464 223 765 984 239 751 17 Cuts at: 5023 5176 5361 5806 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 984 765 751 464 445 239 223 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3594 3651 4110 Size: 274 80 33 51 1443 1617 57 459 Cuts at: 4110 5225 6937 96 Size: 1115 1712 138 Fragments arranged by size: 1712 1617 1443 1115 459 274 138 80 57 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2553 2585 4037 4069 6939 6971 2553 Size: 32 1452 32 2870 32 2561 Fragments arranged by size: 2870 2561 1452 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6979 XbaI T'CTAG_A Cuts at: 4315 4315 Size: 6979 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2792 3334 4151 2792 Size: 542 817 5620 Fragments arranged by size: 5620 817 542 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6979 XmaI C'CCGG_G Cuts at: 1870 2541 2891 4304 1870 Size: 671 350 1413 4545 Fragments arranged by size: 4545 1413 671 350 XmnI GAAnn'nnTTC Cuts at: 3098 3629 4076 5108 3098 Size: 531 447 1032 4969 Fragments arranged by size: 4969 1032 531 447 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4787 276 Size: 53 83 186 936 3253 2468 Fragments arranged by size: 3253 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BplI BsiWI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SrfI SwaI