(Linear) (Six-base) MAPSORT of: KIEE6702.seq Check: 3628 from: 1 to: 2274 KIEE6702.orf With 182 enzymes: SgfI * March 16, 2015 11:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1542 2274 Size: 1542 732 AccI GT'mk_AC Cuts at: 0 1706 2274 Size: 1706 568 AclI AA'CG_TT Cuts at: 0 1871 2274 Size: 1871 403 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1378 1702 1777 1783 1990 2083 2274 Size: 1378 324 75 6 207 93 191 Fragments arranged by size: 1378 324 207 191 93 75 6 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2207 2274 Size: 2207 67 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 626 658 2274 Size: 626 32 1616 Fragments arranged by size: 1616 626 32 AlwNI CAG_nnn'CTG Cuts at: 0 42 870 1137 2274 Size: 42 828 267 1137 Fragments arranged by size: 1137 828 267 42 AseI AT'TA_AT Cuts at: 0 1832 2274 Size: 1832 442 AvaI C'yCGr_G Cuts at: 0 194 536 886 2232 2274 Size: 194 342 350 1346 42 Fragments arranged by size: 1346 350 342 194 42 AvrII C'CTAG_G Cuts at: 0 151 2274 Size: 151 2123 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 232 265 2274 Size: 232 33 2009 Fragments arranged by size: 2009 232 33 BanI G'GyrC_C Cuts at: 0 793 990 1554 1816 2023 2274 Size: 793 197 564 262 207 251 Fragments arranged by size: 793 564 262 251 207 197 BanII G_rGCy'C Cuts at: 0 145 1900 2274 Size: 145 1755 374 Fragments arranged by size: 1755 374 145 BbeI G_GCGC'C Cuts at: 0 1820 2274 Size: 1820 454 BbsI GAAGACnn'nnnn_ Cuts at: 0 381 669 1617 1859 1946 2274 Size: 381 288 948 242 87 328 Fragments arranged by size: 948 381 328 288 242 87 BbvCI CC'TCA_GC Cuts at: 0 1369 1988 2274 Size: 1369 619 286 Fragments arranged by size: 1369 619 286 BciVI GTATCCnnnnn_n' Cuts at: 0 260 2274 Size: 260 2014 BclI T'GATC_A Cuts at: 0 509 2274 Size: 509 1765 BfrBI ATG'CAT Cuts at: 0 2167 2274 Size: 2167 107 BglI GCCn_nnn'nGGC Cuts at: 0 340 490 1411 2274 Size: 340 150 921 863 Fragments arranged by size: 921 863 340 150 BglII A'GATC_T Cuts at: 0 1091 2274 Size: 1091 1183 BlpI GC'TnA_GC Cuts at: 0 144 1378 2274 Size: 144 1234 896 Fragments arranged by size: 1234 896 144 Bme1580I G_kGCm'C Cuts at: 0 995 2028 2156 2274 Size: 995 1033 128 118 Fragments arranged by size: 1033 995 128 118 BmrI ACTGGGnnnn_n' Cuts at: 0 991 1093 2274 Size: 991 102 1181 Fragments arranged by size: 1181 991 102 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 778 1274 2274 Size: 778 496 1000 Fragments arranged by size: 1000 778 496 Bpu10I CC'TnA_GC Cuts at: 0 1369 1899 1988 2274 Size: 1369 530 89 286 Fragments arranged by size: 1369 530 286 89 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 676 1723 2038 2164 2274 Size: 676 1047 315 126 110 Fragments arranged by size: 1047 676 315 126 110 BsaHI Gr'CG_yC Cuts at: 0 1817 2274 Size: 1817 457 BsaWI w'CCGG_w Cuts at: 0 1517 2274 Size: 1517 757 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1375 1405 2274 Size: 1375 30 869 Fragments arranged by size: 1375 869 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 139 1270 1623 1795 1875 2274 Size: 139 1131 353 172 80 399 Fragments arranged by size: 1131 399 353 172 139 80 BseYI C'CCAG_C Cuts at: 0 129 137 591 817 1013 2149 2274 Size: 129 8 454 226 196 1136 125 Fragments arranged by size: 1136 454 226 196 129 125 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 691 2274 Size: 691 1583 BsiEI CG_ry'CG Cuts at: 0 100 2274 Size: 100 2174 BsiHKAI G_wGCw'C Cuts at: 0 187 195 830 1231 1900 2274 Size: 187 8 635 401 669 374 Fragments arranged by size: 669 635 401 374 187 8 BsmBI CGTCTCn'nnnn_ Cuts at: 0 687 2274 Size: 687 1587 Bsp1286I G_dGCh'C Cuts at: 0 145 187 195 830 995 1231 1900 2028 Size: 145 42 8 635 165 236 669 128 Cuts at: 2028 2156 2274 Size: 128 118 Fragments arranged by size: 669 635 236 165 145 128 128 118 42 8 BspEI T'CCGG_A Cuts at: 0 1517 2274 Size: 1517 757 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 77 663 1236 1325 1542 2274 Size: 77 586 573 89 217 732 Fragments arranged by size: 732 586 573 217 89 77 BsrBI CCG'CTC Cuts at: 0 1631 2274 Size: 1631 643 BsrDI GCAATG_nn' Cuts at: 0 68 1070 2274 Size: 68 1002 1204 Fragments arranged by size: 1204 1002 68 BsrGI T'GTAC_A Cuts at: 0 2105 2274 Size: 2105 169 BssSI C'ACGA_G Cuts at: 0 829 1030 1507 2274 Size: 829 201 477 767 Fragments arranged by size: 829 767 477 201 BstAPI GCAn_nnn'nTGC Cuts at: 0 870 2274 Size: 870 1404 BstEII G'GTnAC_C Cuts at: 0 239 274 618 1434 2262 2274 Size: 239 35 344 816 828 12 Fragments arranged by size: 828 816 344 239 35 12 BstXI CCAn_nnnn'nTGG Cuts at: 0 1056 1083 2274 Size: 1056 27 1191 Fragments arranged by size: 1191 1056 27 BstYI r'GATC_y Cuts at: 0 1091 1416 2274 Size: 1091 325 858 Fragments arranged by size: 1091 858 325 Bsu36I CC'TnA_GG Cuts at: 0 1102 2274 Size: 1102 1172 BtgI C'CryG_G Cuts at: 0 428 797 2274 Size: 428 369 1477 Fragments arranged by size: 1477 428 369 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1035 2274 Size: 1035 1239 BtsI GCAGTG_nn' Cuts at: 0 40 187 471 803 2274 Size: 40 147 284 332 1471 Fragments arranged by size: 1471 332 284 147 40 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1013 1048 2274 Size: 1013 35 1226 Fragments arranged by size: 1226 1013 35 DraIII CAC_nnn'GTG Cuts at: 0 562 2048 2274 Size: 562 1486 226 Fragments arranged by size: 1486 562 226 EaeI y'GGCC_r Cuts at: 0 97 299 1445 1637 2274 Size: 97 202 1146 192 637 Fragments arranged by size: 1146 637 202 192 97 EagI C'GGCC_G Cuts at: 0 97 2274 Size: 97 2177 EarI CTCTTCn'nnn_ Cuts at: 0 583 1269 1312 2274 Size: 583 686 43 962 Fragments arranged by size: 962 686 583 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 25 183 1429 2274 Size: 25 158 1246 845 Fragments arranged by size: 1246 845 158 25 EcoICRI GAG'CTC Cuts at: 0 1898 2274 Size: 1898 376 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 778 1274 1378 1702 1777 1783 1990 2083 Size: 778 496 104 324 75 6 207 93 Cuts at: 2083 2274 Size: 191 Fragments arranged by size: 778 496 324 207 191 104 93 75 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 580 1219 1321 1399 1549 2274 Size: 580 639 102 78 150 725 Fragments arranged by size: 725 639 580 150 102 78 EcoO109I rG'GnC_Cy Cuts at: 0 155 434 1950 1974 2274 Size: 155 279 1516 24 300 Fragments arranged by size: 1516 300 279 155 24 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2205 2274 Size: 2205 69 HaeII r_GCGC'y Cuts at: 0 138 1043 1820 2274 Size: 138 905 777 454 Fragments arranged by size: 905 777 454 138 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 268 300 1375 1407 2274 Size: 268 32 1075 32 867 Fragments arranged by size: 1075 867 268 32 32 HincII GTy'rAC Cuts at: 0 1707 1839 2274 Size: 1707 132 435 Fragments arranged by size: 1707 435 132 HindIII A'AGCT_T Cuts at: 0 1786 2274 Size: 1786 488 KasI G'GCGC_C Cuts at: 0 1816 2274 Size: 1816 458 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 240 2250 2274 Size: 240 2010 24 Fragments arranged by size: 2010 240 24 MscI TGG'CCA Cuts at: 0 301 2274 Size: 301 1973 MslI CAynn'nnrTG Cuts at: 0 427 451 1054 1348 2274 Size: 427 24 603 294 926 Fragments arranged by size: 926 603 427 294 24 MspA1I CmG'CkG Cuts at: 0 102 661 667 811 870 1017 1755 2274 Size: 102 559 6 144 59 147 738 519 Fragments arranged by size: 738 559 519 147 144 102 59 6 NarI GG'CG_CC Cuts at: 0 1817 2274 Size: 1817 457 NsiI A_TGCA'T Cuts at: 0 2169 2274 Size: 2169 105 NspI r_CATG'y Cuts at: 0 1368 1389 2274 Size: 1368 21 885 Fragments arranged by size: 1368 885 21 PasI CC'CwG_GG Cuts at: 0 1146 1977 2274 Size: 1146 831 297 Fragments arranged by size: 1146 831 297 PflMI CCAn_nnn'nTGG Cuts at: 0 1137 2274 Size: 1137 1137 PfoI T'CCnGG_A Cuts at: 0 2012 2084 2274 Size: 2012 72 190 Fragments arranged by size: 2012 190 72 PpuMI rG'GwC_Cy Cuts at: 0 155 434 2274 Size: 155 279 1840 Fragments arranged by size: 1840 279 155 PstI C_TGCA'G Cuts at: 0 666 810 863 984 1775 2274 Size: 666 144 53 121 791 499 Fragments arranged by size: 791 666 499 144 121 53 PvuII CAG'CTG Cuts at: 0 661 667 811 870 1017 1755 2274 Size: 661 6 144 59 147 738 519 Fragments arranged by size: 738 661 519 147 144 59 6 SacI G_AGCT'C Cuts at: 0 1900 2274 Size: 1900 374 SalI G'TCGA_C Cuts at: 0 1705 2274 Size: 1705 569 SbfI CC_TGCA'GG Cuts at: 0 984 2274 Size: 984 1290 ScaI AGT'ACT Cuts at: 0 1591 2274 Size: 1591 683 SexAI A'CCwGG_T Cuts at: 0 172 2274 Size: 172 2102 SfcI C'TryA_G Cuts at: 0 369 662 806 859 980 1668 1771 2274 Size: 369 293 144 53 121 688 103 503 Fragments arranged by size: 688 503 369 293 144 121 103 53 SfiI GGCCn_nnn'nGGCC Cuts at: 0 490 2274 Size: 490 1784 SfoI GGC'GCC Cuts at: 0 1818 2274 Size: 1818 456 SmaI CCC'GGG Cuts at: 0 538 888 2274 Size: 538 350 1386 Fragments arranged by size: 1386 538 350 SmlI C'TyrA_G Cuts at: 0 691 1738 2053 2143 2274 Size: 691 1047 315 90 131 Fragments arranged by size: 1047 691 315 131 90 SphI G_CATG'C Cuts at: 0 1368 2274 Size: 1368 906 SspI AAT'ATT Cuts at: 0 2070 2274 Size: 2070 204 StuI AGG'CCT Cuts at: 0 493 2238 2274 Size: 493 1745 36 Fragments arranged by size: 1745 493 36 StyI C'CwwG_G Cuts at: 0 151 1322 1470 1601 1811 1946 2274 Size: 151 1171 148 131 210 135 328 Fragments arranged by size: 1171 328 210 151 148 135 131 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 39 262 1027 2011 2250 2274 Size: 39 223 765 984 239 24 Fragments arranged by size: 984 765 239 223 39 24 TatI w'GTAC_w Cuts at: 0 1589 1646 2105 2274 Size: 1589 57 459 169 Fragments arranged by size: 1589 459 169 57 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 548 580 2032 2064 2274 Size: 548 32 1452 32 210 Fragments arranged by size: 1452 548 210 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 787 1329 2146 2274 Size: 787 542 817 128 Fragments arranged by size: 817 787 542 128 XmaI C'CCGG_G Cuts at: 0 536 886 2274 Size: 536 350 1388 Fragments arranged by size: 1388 536 350 XmnI GAAnn'nnTTC Cuts at: 0 1093 1624 2071 2274 Size: 1093 531 447 203 Fragments arranged by size: 1093 531 447 203 Enzymes that do cut: AarI AccI AclI AcuI AlfI AloI AlwNI AseI AvaI AvrII BaeI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I NarI NsiI NspI PasI PflMI PfoI PpuMI PstI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SfoI SmaI SmlI SphI SspI StuI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AfeI AflII AflIII AgeI AhdI AleI ApaI ApaLI ApoI AscI AsiSI BamHI BcgI BmgBI BmtI BplI BsaI BsaAI BsaBI BsiWI BsmI BspHI BsrFI BssHII BstBI BstZ17I ClaI DraI DrdI EcoRI EcoRV FseI FspI FspAI HpaI KpnI MfeI MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SanDI SapI SgfI SgrAI SnaBI SpeI SrfI SwaI Tth111I XbaI XhoI ZraI