Restriction map for
pFN21AB0986
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
Acc65I AccII AccIII AclI AcsI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BamHI BbeI BbrPI
BciVI BfrI BlnI BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmFI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BstAPI BstBI
BstEII BstPI BstUI BstXI Bsu36I
ClaI CpoI DraI DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HgaI HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MslI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI Tth111I
TthHB8I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 262 GACGTC GACGT^C
AccI 156 GTATAC GT^[AC][GT]AC
AcyI 262 GACGTC G[GA]^CG[CT]C
AhdI 1114 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1114 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 435 CCCGGG C^[CT]CG[GA]G
BalI 417 TGGCCA TGG^CCA
BbsI 105 GAAGAC GAAGAC(2/6)
BbvCI 942 CCTCAGC CCTCAGC(-5/-2)
BcgI 231 CGAACAACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 754 GCCGAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 105 GAAGAC GAAGAC(2/6)
BsaHI 262 GACGTC G[GA]^CG[CT]C
BsaI 985 GAGACC GGTCTC(1/5)
BsmBI 934 GAGACG CGTCTC(1/5)
BsmI 358 GAATGC GAATGC(1/-1)
BsoBI 435 CCCGGG C^[CT]CG[GA]G
BspMI 988 ACCTGC ACCTGC(4/8)
BsrBI 634 GAGCGG CCGCTC(-3/-3)
BsrFI 459 ACCGGC [GA]^CCGG[CT]
Bst1107I 156 GTATAC GTA^TAC
BstF5I 793 CATCC GGATG(2/0)
BstZ17I 156 GTATAC GTA^TAC
Cfr10I 459 ACCGGC [GA]^CCGG[CT]
DsaI 49 CCGTGG C^C[GA][CT]GG
EaeI 417 TGGCCA [CT]^GGCC[GA]
Eam1105I 1114 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 645 GCGGG CCCGC(4/6)
FokI 793 CATCC GGATG(9/13)
Hin1I 262 GACGTC G[GA]^CG[CT]C
HincII 1222 GTCAAC GT[CT]^[GA]AC
HindII 1222 GTCAAC GT[CT]^[GA]AC
MaeII 263 ACGT A^CGT
MluNI 417 TGGCCA TGG^CCA
MscI 417 TGGCCA TGG^CCA
PflMI 1199 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1151 CTGCAG CTGCA^G
SexAI 14 ACCTGGT A^CC[AT]GGT
SfcI 1151 CTGCAG C^T[GA][CT]AG
SmaI 435 CCCGGG CCC^GGG
SmlI 916 CTCAAG C^T[CT][GA]AG
StuI 317 AGGCCT AGG^CCT
Van91I 1199 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 435 CCCGGG C^CCGGG
XmaI 435 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AfaI 54 GTAC GT^AC
1056 GTAC
AlwI 939 GATCC GGATC(4/5)
117 GGATC
AspHI 586 GAGCAC G[AT]GC[AT]^C
731 GAGCAC
AvaII 66 GGACC G^G[AT]CC
1161 GGACC
BclI 378 TGATCA T^GATCA
519 TGATCA
BcnI 435 CCCGG CC^[GC]GG
436 CCGGG
BfaI 301 CTAG C^TAG
748 CTAG
BglII 78 AGATCT A^GATCT
1283 AGATCT
BlpI 94 GCTGAGC GC^T[ACGT]AGC
624 GCTGAGC
Bpu1102I 94 GCTGAGC GC^T[ACGT]AGC
624 GCTGAGC
BsgI 1036 CTGCAC GTGCAG(16/14)
1135 GTGCAG
BsiHKAI 586 GAGCAC G[AT]GC[AT]^C
731 GAGCAC
BsmAI 934 GAGAC GTCTC(1/5)
985 GAGAC
BssSI 979 CACGAG CACGAG(-5/-1)
673 CTCGTG
BstYI 78 AGATCT [GA]^GATC[CT]
1283 AGATCT
CelII 94 GCTGAGC GC^T[ACGT]AGC
624 GCTGAGC
DraII 65 AGGACCT [GA]G^G[ACGT]CC[CT]
1160 AGGACCT
DraIII 508 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
1039 CACGAAGTG
EcoNI 15 CCTGGTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
989 CCTGCATCAGG
EcoO109I 65 AGGACCT [GA]G^G[ACGT]CC[CT]
1160 AGGACCT
FbaI 378 TGATCA T^GATCA
519 TGATCA
HaeII 738 GGCGCT [GA]GCGC^[CT]
1060 AGCGCC
MaeI 301 CTAG C^TAG
748 CTAG
MflI 78 AGATCT [GA]^GATC[CT]
1283 AGATCT
MseI 679 TTAA T^TAA
1277 TTAA
NciI 435 CCCGG CC^[GC]GG
436 CCGGG
NspI 237 ACATGC [GA]CATG^[CT]
475 GCATGT
PpuMI 65 AGGACCT [GA]G^G[AT]CC[CT]
1160 AGGACCT
PvuII 622 CAGCTG CAG^CTG
706 CAGCTG
RsaI 54 GTAC GT^AC
1056 GTAC
TfiI 333 GATTC G^A[AT]TC
898 GAATC
Tru9I 679 TTAA T^TAA
1277 TTAA
Tsp509I 186 AATT ^AATT
257 AATT
XhoII 78 AGATCT [GA]^GATC[CT]
1283 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AlwNI BanI BanII
BbvI BmyI BpmI BsaJI BseRI
BsiYI BslI Bsp1286I BsrI BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
StyI TseI Tsp45I TspRI