(Circular) (Six-base) MAPSORT of: pFN21AB0986.seq Check: 5730 from: 1 to: 6067 pFN21AB0986 6067 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2271 3877 278 Size: 53 83 186 936 735 1606 2468 Fragments arranged by size: 2468 1606 936 735 186 83 53 AccI GT'mk_AC Cuts at: 1937 2162 3410 1937 Size: 225 1248 4594 Fragments arranged by size: 4594 1248 225 Acc65I G'GTAC_C Cuts at: 3388 3388 Size: 6067 AclI AA'CG_TT Cuts at: 1563 4194 4567 1563 Size: 2631 373 3063 Fragments arranged by size: 3063 2631 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2432 2507 3092 3116 4130 5514 5782 1632 Size: 800 75 585 24 1014 1384 268 1917 Fragments arranged by size: 1917 1384 1014 800 585 268 75 24 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5870 AflIII A'CryG_T Cuts at: 1203 4966 5943 1203 Size: 3763 977 1327 Fragments arranged by size: 3763 1327 977 AgeI A'CCGG_T Cuts at: 4898 4898 Size: 6067 AhdI GACnn_n'nnGTC Cuts at: 3124 4796 3124 Size: 1672 4395 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6067 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6067 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2870 2902 2870 Size: 32 6035 AlwNI CAG_nnn'CTG Cuts at: 2713 2872 3210 3810 5382 2713 Size: 159 338 600 1572 3398 Fragments arranged by size: 3398 1572 600 338 159 ApaLI G'TGCA_C Cuts at: 4124 5280 4124 Size: 1156 4911 ApoI r'AATT_y Cuts at: 1493 3376 3602 3754 5863 1493 Size: 1883 226 152 2109 1697 Fragments arranged by size: 2109 1883 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4621 160 Size: 872 3589 1606 Fragments arranged by size: 3589 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 AvaI C'yCGr_G Cuts at: 1870 1943 2440 3392 1870 Size: 73 497 952 4545 Fragments arranged by size: 4545 952 497 73 BamHI G'GATC_C Cuts at: 3397 3764 3397 Size: 367 5700 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2467 2726 2827 3388 4843 Size: 325 217 627 680 259 101 561 1455 Cuts at: 4843 618 Size: 1842 Fragments arranged by size: 1842 1455 680 627 561 325 259 217 101 BanII G_rGCy'C Cuts at: 1391 2441 2679 2862 3240 3386 1391 Size: 1050 238 183 378 146 4072 Fragments arranged by size: 4072 1050 378 238 183 146 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2117 928 Size: 971 218 4878 Fragments arranged by size: 4878 971 218 BbvCI CC'TCA_GC Cuts at: 2948 2948 Size: 6067 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2225 2259 4258 4292 1903 Size: 34 50 34 204 34 1999 34 3678 Fragments arranged by size: 3678 1999 204 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3959 5169 3959 Size: 1210 4857 BclI T'GATC_A Cuts at: 1548 2383 2524 1548 Size: 835 141 5091 Fragments arranged by size: 5091 835 141 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2765 4678 Size: 107 122 71 631 605 337 756 1913 Cuts at: 4678 136 Size: 1525 Fragments arranged by size: 1913 1525 756 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2083 3288 6062 2083 Size: 1205 2774 2088 Fragments arranged by size: 2774 2088 1205 BlpI GC'TnA_GC Cuts at: 2100 2630 3484 2100 Size: 530 854 4683 Fragments arranged by size: 4683 854 530 Bme1580I G_kGCm'C Cuts at: 1790 4128 5284 1790 Size: 2338 1156 2573 Fragments arranged by size: 2573 2338 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6067 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3181 3798 4756 Size: 416 53 83 399 24 1764 617 958 Cuts at: 4756 442 Size: 1753 Fragments arranged by size: 1764 1753 958 617 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6067 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 2375 2657 2723 3133 3195 3246 4727 Size: 821 315 282 66 410 62 51 1481 Cuts at: 4727 1239 Size: 2579 Fragments arranged by size: 2579 1481 821 410 315 282 66 62 51 Bpu10I CC'TnA_GC Cuts at: 2948 2948 Size: 6067 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2906 4192 5057 5355 2906 Size: 1286 865 298 3618 Fragments arranged by size: 3618 1286 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2984 4730 882 Size: 617 1485 1746 2219 Fragments arranged by size: 2219 1746 1485 617 BsaAI yAC'GTr Cuts at: 493 1204 3858 493 Size: 711 2654 2702 Fragments arranged by size: 2702 2654 711 BsaBI GATnn'nnATC Cuts at: 6061 6061 Size: 6067 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2268 3874 4256 4891 Size: 53 83 186 936 735 1606 382 635 Cuts at: 4891 275 Size: 1451 Fragments arranged by size: 1606 1451 936 735 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4500 4898 5172 5319 5730 4500 Size: 398 274 147 411 4837 Fragments arranged by size: 4837 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2399 2429 3244 3274 1178 Size: 30 165 30 996 30 815 30 3971 Fragments arranged by size: 3971 996 815 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 2420 2678 3002 3346 1186 Size: 856 378 258 324 344 3907 Fragments arranged by size: 3907 856 378 344 324 258 BseYI C'CCAG_C Cuts at: 2471 2625 2713 2861 3210 5270 5766 2471 Size: 154 88 148 349 2060 496 2772 Fragments arranged by size: 2772 2060 496 349 154 148 88 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3026 3161 1163 Size: 573 1290 135 4069 Fragments arranged by size: 4069 1290 573 135 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3517 4278 5306 1540 Size: 275 186 1516 761 1028 2301 Fragments arranged by size: 2301 1516 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2595 2740 4128 4213 5284 2595 Size: 145 1388 85 1071 3378 Fragments arranged by size: 3378 1388 1071 145 85 BsmI GAATG_Cn' Cuts at: 2369 3578 3671 2369 Size: 1209 93 4765 Fragments arranged by size: 4765 1209 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2933 2933 Size: 6067 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2441 2595 2679 2740 2862 3240 3386 Size: 399 651 154 84 61 122 378 146 Cuts at: 3386 4128 4213 5284 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 651 399 378 154 146 122 85 84 61 BspEI T'CCGG_A Cuts at: 5730 5730 Size: 6067 BspHI T'CATG_A Cuts at: 3956 5686 3956 Size: 1730 4337 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3002 3422 844 Size: 2158 420 3489 Fragments arranged by size: 3489 2158 420 BsrBI CCG'CTC Cuts at: 1394 1823 2641 3954 1394 Size: 429 818 1313 3507 Fragments arranged by size: 3507 1313 818 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4562 4736 65 Size: 1183 3314 174 1396 Fragments arranged by size: 3314 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2464 4711 4898 1700 Size: 110 654 2247 187 2869 Fragments arranged by size: 2869 2247 654 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6067 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6067 BssSI C'ACGA_G Cuts at: 2678 2984 4127 5139 2678 Size: 306 1143 1012 3606 Fragments arranged by size: 3606 1143 1012 306 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6067 BstBI TT'CG_AA Cuts at: 4871 4871 Size: 6067 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6067 BstYI r'GATC_y Cuts at: 1972 2083 3288 3397 3764 4149 4166 5618 5704 Size: 111 1205 109 367 385 17 1452 86 Cuts at: 5704 5716 6062 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1205 385 367 346 111 109 86 17 12 BstZ17I GTA'TAC Cuts at: 2163 2163 Size: 6067 BtgI C'CryG_G Cuts at: 513 1065 2054 513 Size: 552 989 4526 Fragments arranged by size: 4526 989 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5328 Fragments arranged by size: 5328 683 56 BtsI GCAGTG_nn' Cuts at: 2941 3583 4377 4397 2941 Size: 642 794 20 4611 Fragments arranged by size: 4611 794 642 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6067 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6032 DraI TTT'AAA Cuts at: 3371 3371 Size: 6067 DraIII CAC_nnn'GTG Cuts at: 2518 3049 2518 Size: 531 5536 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5074 809 Size: 507 3758 1802 Fragments arranged by size: 3758 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2422 3514 4403 Size: 54 1425 50 275 18 592 1092 889 Cuts at: 4403 6032 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1092 889 592 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3514 1537 Size: 275 1702 4090 Fragments arranged by size: 4090 1702 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2574 2874 3081 3997 1353 Size: 1221 300 207 916 3423 Fragments arranged by size: 3423 1221 916 300 207 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2027 2495 4654 5028 5174 387 Size: 1064 138 438 468 2159 374 146 1280 Fragments arranged by size: 2159 1280 1064 468 438 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2375 2432 2507 2657 2723 3092 Size: 393 428 315 57 75 150 66 369 Cuts at: 3092 3116 3133 3195 3246 4130 4727 5514 5782 Size: 24 17 62 51 884 597 787 268 Cuts at: 5782 1239 Size: 1524 Fragments arranged by size: 1524 884 787 597 428 393 369 315 268 150 75 66 62 57 51 24 17 EcoNI CCTnn'n_nnAGG Cuts at: 2024 2998 2024 Size: 974 5093 EcoO109I rG'GnC_Cy Cuts at: 2071 3166 2071 Size: 1095 4972 EcoRI G'AATT_C Cuts at: 3376 3754 3376 Size: 378 5689 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2234 3307 2234 Size: 1073 4994 FspI TGC'GCA Cuts at: 4573 4573 Size: 6067 HaeII r_GCGC'y Cuts at: 1678 2747 3069 5214 1678 Size: 1069 322 2145 2531 Fragments arranged by size: 2531 2145 1069 322 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2399 2431 3110 3142 4708 Size: 32 135 32 827 32 679 32 1566 Cuts at: 4708 4740 4782 4814 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1566 827 679 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3229 3411 3657 669 Size: 988 281 1291 182 246 3079 Fragments arranged by size: 3079 1291 988 281 246 182 HindIII A'AGCT_T Cuts at: 748 748 Size: 6067 HpaI GTT'AAC Cuts at: 3657 3657 Size: 6067 KpnI G_GTAC'C Cuts at: 3392 3392 Size: 6067 MfeI C'AATT_G Cuts at: 3666 3666 Size: 6067 MluI A'CGCG_T Cuts at: 5943 5943 Size: 6067 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2118 5181 5365 1305 Size: 813 3063 184 2007 Fragments arranged by size: 3063 2007 813 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2424 10 Size: 54 1425 343 592 3653 Fragments arranged by size: 3653 1425 592 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4025 4384 4543 518 Size: 648 2859 359 159 2042 Fragments arranged by size: 2859 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2629 2713 3207 3483 4160 5308 5553 1606 Size: 1023 84 494 276 677 1148 245 2120 Fragments arranged by size: 2120 1148 1023 677 494 276 245 84 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5515 NdeI CA'TA_TG Cuts at: 387 387 Size: 6067 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6067 NotI GC'GGCC_GC Cuts at: 3514 3514 Size: 6067 NspI r_CATG'y Cuts at: 1132 1229 2246 2484 3425 4970 1132 Size: 97 1017 238 941 1545 2229 Fragments arranged by size: 2229 1545 1017 941 238 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6067 PciI A'CATG_T Cuts at: 4966 4966 Size: 6067 PflMI CCAn_nnn'nTGG Cuts at: 1108 3210 1108 Size: 2102 3965 PmeI GTTT'AAAC Cuts at: 3371 3371 Size: 6067 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4136 4168 5649 5681 4136 Size: 32 1481 32 4522 Fragments arranged by size: 4522 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2071 3166 2071 Size: 1095 4972 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6067 PsiI TTA'TAA Cuts at: 3637 3637 Size: 6067 PstI C_TGCA'G Cuts at: 830 3160 3419 830 Size: 2330 259 3478 Fragments arranged by size: 3478 2330 259 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6067 PvuII CAG'CTG Cuts at: 2629 2713 2629 Size: 84 5983 SalI G'TCGA_C Cuts at: 1936 3409 1936 Size: 1473 4594 SbfI CC_TGCA'GG Cuts at: 3419 3419 Size: 6067 ScaI AGT'ACT Cuts at: 4315 6027 4315 Size: 1712 4355 SexAI A'CCwGG_T Cuts at: 2019 2019 Size: 6067 SfcI C'TryA_G Cuts at: 826 1045 3156 3415 4550 5231 5422 826 Size: 219 2111 259 1135 681 191 1471 Fragments arranged by size: 2111 1471 1135 681 259 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6067 SmaI CCC'GGG Cuts at: 1872 2442 3394 1872 Size: 570 952 4545 Fragments arranged by size: 4545 952 570 SmlI C'TyrA_G Cuts at: 820 1017 1943 2921 4171 5072 5334 820 Size: 197 926 978 1250 901 262 1553 Fragments arranged by size: 1553 1250 978 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3858 493 Size: 3365 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6067 SphI G_CATG'C Cuts at: 1132 3425 1132 Size: 2293 3774 SspI AAT'ATT Cuts at: 5 52 3991 5 Size: 47 3939 2081 Fragments arranged by size: 3939 2081 47 StuI AGG'CCT Cuts at: 1517 2324 1517 Size: 807 5260 StyI C'CwwG_G Cuts at: 513 1065 2425 3009 3162 3506 513 Size: 552 1360 584 153 344 3074 Fragments arranged by size: 3074 1360 584 552 344 153 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2485 4094 4111 4264 4449 4894 325 Size: 1255 905 1609 17 153 185 445 1498 Fragments arranged by size: 1609 1498 1255 905 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3060 4313 6025 Size: 274 80 33 51 1443 1083 1253 1712 Cuts at: 6025 96 Size: 138 Fragments arranged by size: 1712 1443 1253 1083 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2039 2071 6027 6059 2039 Size: 32 3956 32 2047 Fragments arranged by size: 3956 2047 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6067 XbaI T'CTAG_A Cuts at: 3403 3403 Size: 6067 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6067 XmaI C'CCGG_G Cuts at: 1870 2440 3392 1870 Size: 570 952 4545 Fragments arranged by size: 4545 952 570 XmnI GAAnn'nnTTC Cuts at: 4196 4196 Size: 6067 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2269 3875 276 Size: 53 83 186 936 735 1606 2468 Fragments arranged by size: 2468 1606 936 735 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI