Restriction map for
pFN21AB0986
(Linear) (Six-base) MAPSORT of: KIBB0986.seq Check: 4549 from: 1 to: 1362
KIBB0986.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AatII G_ACGT'C
Cuts at: 0 266 1362
Size: 266 1096
AccI GT'mk_AC
Cuts at: 0 157 1362
Size: 157 1205
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 502 1087 1111 1362
Size: 427 75 585 24 251
Fragments arranged by size:
585 427 251 75 24
AhdI GACnn_n'nnGTC
Cuts at: 0 1119 1362
Size: 1119 243
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 865 897 1362
Size: 865 32 465
Fragments arranged by size:
865 465 32
AlwNI CAG_nnn'CTG
Cuts at: 0 708 867 1205 1362
Size: 708 159 338 157
Fragments arranged by size:
708 338 159 157
AvaI C'yCGr_G
Cuts at: 0 435 1362
Size: 435 927
BanI G'GyrC_C
Cuts at: 0 462 721 822 1362
Size: 462 259 101 540
Fragments arranged by size:
540 462 259 101
BanII G_rGCy'C
Cuts at: 0 436 674 857 1235 1362
Size: 436 238 183 378 127
Fragments arranged by size:
436 378 238 183 127
BbsI GAAGACnn'nnnn_
Cuts at: 0 112 1362
Size: 112 1250
BbvCI CC'TCA_GC
Cuts at: 0 943 1362
Size: 943 419
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 220 254 1362
Size: 220 34 1108
Fragments arranged by size:
1108 220 34
BclI T'GATC_A
Cuts at: 0 378 519 1362
Size: 378 141 843
Fragments arranged by size:
843 378 141
BglI GCCn_nnn'nGGC
Cuts at: 0 760 1362
Size: 760 602
BglII A'GATC_T
Cuts at: 0 78 1283 1362
Size: 78 1205 79
Fragments arranged by size:
1205 79 78
BlpI GC'TnA_GC
Cuts at: 0 95 625 1362
Size: 95 530 737
Fragments arranged by size:
737 530 95
BmrI ACTGGGnnnn_n'
Cuts at: 0 1176 1362
Size: 1176 186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 370 652 718 1128 1190 1241 1362
Size: 55 315 282 66 410 62 51 121
Fragments arranged by size:
410 315 282 121 66 62 55 51
Bpu10I CC'TnA_GC
Cuts at: 0 943 1362
Size: 943 419
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 1362
Size: 901 461
BsaI GGTCTCn'nnnn_
Cuts at: 0 979 1362
Size: 979 383
BsaHI Gr'CG_yC
Cuts at: 0 263 1362
Size: 263 1099
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 394 424 1239 1269 1362
Size: 394 30 815 30 93
Fragments arranged by size:
815 394 93 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 415 673 997 1341 1362
Size: 37 378 258 324 344 21
Fragments arranged by size:
378 344 324 258 37 21
BseYI C'CCAG_C
Cuts at: 0 466 620 708 856 1205 1362
Size: 466 154 88 148 349 157
Fragments arranged by size:
466 349 157 154 148 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1021 1156 1362
Size: 1021 135 206
Fragments arranged by size:
1021 206 135
BsiHKAI G_wGCw'C
Cuts at: 0 590 735 1362
Size: 590 145 627
Fragments arranged by size:
627 590 145
BsmI GAATG_Cn'
Cuts at: 0 364 1362
Size: 364 998
BsmBI CGTCTCn'nnnn_
Cuts at: 0 928 1362
Size: 928 434
Bsp1286I G_dGCh'C
Cuts at: 0 436 590 674 735 857 1235 1362
Size: 436 154 84 61 122 378 127
Fragments arranged by size:
436 378 154 127 122 84 61
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 997 1362
Size: 997 365
BsrBI CCG'CTC
Cuts at: 0 636 1362
Size: 636 726
BsrFI r'CCGG_y
Cuts at: 0 459 1362
Size: 459 903
BssSI C'ACGA_G
Cuts at: 0 673 979 1362
Size: 673 306 383
Fragments arranged by size:
673 383 306
BstYI r'GATC_y
Cuts at: 0 78 1283 1362
Size: 78 1205 79
Fragments arranged by size:
1205 79 78
BstZ17I GTA'TAC
Cuts at: 0 158 1362
Size: 158 1204
BtgI C'CryG_G
Cuts at: 0 49 1362
Size: 49 1313
BtsI GCAGTG_nn'
Cuts at: 0 936 1362
Size: 936 426
DraIII CAC_nnn'GTG
Cuts at: 0 513 1044 1362
Size: 513 531 318
Fragments arranged by size:
531 513 318
EaeI y'GGCC_r
Cuts at: 0 417 1362
Size: 417 945
EarI CTCTTCn'nnn_
Cuts at: 0 569 869 1076 1362
Size: 569 300 207 286
Fragments arranged by size:
569 300 286 207
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 22 490 1362
Size: 22 468 872
Fragments arranged by size:
872 468 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 370 427 502 652 718 1087 1111
Size: 55 315 57 75 150 66 369 24
Cuts at: 1111 1128 1190 1241 1362
Size: 17 62 51 121
Fragments arranged by size:
369 315 150 121 75 66 62 57
55 51 24 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 19 993 1362
Size: 19 974 369
Fragments arranged by size:
974 369 19
EcoO109I rG'GnC_Cy
Cuts at: 0 66 1161 1362
Size: 66 1095 201
Fragments arranged by size:
1095 201 66
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 229 1302 1362
Size: 229 1073 60
Fragments arranged by size:
1073 229 60
HaeII r_GCGC'y
Cuts at: 0 742 1064 1362
Size: 742 322 298
Fragments arranged by size:
742 322 298
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 394 426 1105 1137 1362
Size: 394 32 679 32 225
Fragments arranged by size:
679 394 225 32 32
HincII GTy'rAC
Cuts at: 0 1224 1362
Size: 1224 138
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 1362
Size: 113 1249
MscI TGG'CCA
Cuts at: 0 419 1362
Size: 419 943
MspA1I CmG'CkG
Cuts at: 0 624 708 1202 1362
Size: 624 84 494 160
Fragments arranged by size:
624 494 160 84
NspI r_CATG'y
Cuts at: 0 241 479 1362
Size: 241 238 883
Fragments arranged by size:
883 241 238
PflMI CCAn_nnn'nTGG
Cuts at: 0 1205 1362
Size: 1205 157
PpuMI rG'GwC_Cy
Cuts at: 0 66 1161 1362
Size: 66 1095 201
Fragments arranged by size:
1095 201 66
PstI C_TGCA'G
Cuts at: 0 1155 1362
Size: 1155 207
PvuII CAG'CTG
Cuts at: 0 624 708 1362
Size: 624 84 654
Fragments arranged by size:
654 624 84
SexAI A'CCwGG_T
Cuts at: 0 14 1362
Size: 14 1348
SfcI C'TryA_G
Cuts at: 0 1151 1362
Size: 1151 211
SmaI CCC'GGG
Cuts at: 0 437 1362
Size: 437 925
SmlI C'TyrA_G
Cuts at: 0 916 1362
Size: 916 446
StuI AGG'CCT
Cuts at: 0 319 1362
Size: 319 1043
StyI C'CwwG_G
Cuts at: 0 420 1004 1157 1362
Size: 420 584 153 205
Fragments arranged by size:
584 420 205 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 480 1362
Size: 480 882
TatI w'GTAC_w
Cuts at: 0 1055 1362
Size: 1055 307
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 34 66 1362
Size: 34 32 1296
Fragments arranged by size:
1296 34 32
XmaI C'CCGG_G
Cuts at: 0 435 1362
Size: 435 927
ZraI GAC'GTC
Cuts at: 0 264 1362
Size: 264 1098
Enzymes that do cut:
AatII AccI AcuI AhdI AloI AlwNI AvaI BanI
BanII BbsI BbvCI BcgI BclI BglI BglII BlpI
BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssSI BstYI BstZ17I BtgI BtsI DraIII EaeI
EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII MmeI MscI MspA1I NspI PflMI PpuMI PstI
PvuII SexAI SfcI SmaI SmlI StuI StyI TaqII
TatI TstI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AleI
AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BciVI BfrBI Bme1580I BmgBI BmtI
BplI BsaAI BsaBI BsaWI BsiEI BsiWI BspEI BspHI
BsrDI BsrGI BssHII BstAPI BstBI BstEII BstXI Bsu36I
BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoRI
EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmnI