Restriction map for
pFN21AE6429
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspI AviII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfrI BglI BlpI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI DsaI EaeI EagI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HapII HhaI Hin1I HinP1I
HincII HindII HpaI HpaII KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SwaI
Tth111I XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 870 GTCTAC GT^[AC][GT]AC
AciI 1581 GCGG CCGC(-3/-1)
AcsI 255 AAATTT [GA]^AATT[CT]
AflIII 313 ACATGT A^C[GA][CT]GT
AhdI 862 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 33 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 550 CCTCAGG CC^T[ACGT]AGG
ApaI 169 GGGCCC GGGCC^C
ApoI 255 AAATTT [GA]^AATT[CT]
AspEI 862 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 6 CTCGAG C^[CT]CG[GA]G
AvrII 1671 CCTAGG C^CTAGG
BanI 542 GGTGCC G^G[CT][GA]CC
BclI 534 TGATCA T^GATCA
BglII 1421 AGATCT A^GATCT
BlnI 1671 CCTAGG C^CTAGG
BpmI 125 CTCCAG CTGGAG(16/14)
BsaBI 808 GATCTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 22 GAGACG CGTCTC(1/5)
BsmFI 1437 GTCCC GGGAC(10/14)
BsmI 1310 GAATGC GAATGC(1/-1)
BsoBI 6 CTCGAG C^[CT]CG[GA]G
BspHI 1509 TCATGA T^CATGA
BspLU11I 313 ACATGT A^CATGT
Bsu36I 550 CCTCAGG CC^T[ACGT]AGG
DraI 300 TTTAAA TTT^AAA
Eam1105I 862 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 550 CCTCAGG CC^T[ACGT]AGG
FbaI 534 TGATCA T^GATCA
HgaI 1554 GCGTC GACGC(5/10)
MaeII 1278 ACGT A^CGT
MamI 808 GATCTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 36 CAGCTG C[AC]G^C[GT]G
NspI 313 ACATGT [GA]CATG^[CT]
PaeR7I 6 CTCGAG C^TCGAG
PshAI 1430 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 36 CAGCTG CAG^CTG
RcaI 1509 TCATGA T^CATGA
SacI 3 GAGCTC GAGCT^C
ScaI 325 AGTACT AGT^ACT
SexAI 1707 ACCAGGT A^CC[AT]GGT
SfaNI 1132 GCATC GCATC(5/9)
SpeI 987 ACTAGT A^CTAGT
SspI 1268 AATATT AAT^ATT
XhoI 6 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AlwI 700 GGATC GGATC(4/5)
807 GGATC
AspHI 3 GAGCTC G[AT]GC[AT]^C
220 GTGCTC
BanII 3 GAGCTC G[GA]GC[CT]^C
169 GGGCCC
BbsI 886 GAAGAC GAAGAC(2/6)
1747 GAAGAC
BpuAI 886 GAAGAC GAAGAC(2/6)
1747 GAAGAC
BseRI 243 CTCCTC GAGGAG(10/8)
1018 GAGGAG
BsgI 602 CTGCAC GTGCAG(16/14)
187 GTGCAG
BsiHKAI 3 GAGCTC G[AT]GC[AT]^C
220 GTGCTC
BspMI 720 GCAGGT ACCTGC(4/8)
1314 GCAGGT
BstYI 807 GGATCT [GA]^GATC[CT]
1421 AGATCT
DraII 157 AGGACCT [GA]G^G[ACGT]CC[CT]
1435 AGGTCCC
EarI 1220 CTCTTC CTCTTC(1/4)
1531 GAAGAG
EcoO109I 157 AGGACCT [GA]G^G[ACGT]CC[CT]
1435 AGGTCCC
EcoT14I 194 CCTTGG C^C[AT][AT]GG
1671 CCTAGG
HindIII 1504 AAGCTT A^AGCTT
1521 AAGCTT
HphI 723 GGTGA GGTGA(8/7)
1152 TCACC
Ksp632I 1220 CTCTTC CTCTTC(1/4)
1531 GAAGAG
MaeIII 978 GTCAC ^GT[ACGT]AC
1490 GTGAC
MflI 807 GGATCT [GA]^GATC[CT]
1421 AGATCT
MslI 1380 CACGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1456 CATAAAGATG
MwoI 29 GCAGCAGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
69 GCAGTGCCAGC
PflMI 252 CCAAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1194 CCAGGCATTGG
PpuMI 157 AGGACCT [GA]G^G[AT]CC[CT]
1435 AGGTCCC
StuI 191 AGGCCT AGG^CCT
1674 AGGCCT
StyI 194 CCTTGG C^C[AT][AT]GG
1671 CCTAGG
TaqI 7 TCGA T^CGA
1224 TCGA
Tsp45I 978 GTCAC ^GT[GC]AC
1490 GTGAC
TthHB8I 7 TCGA T^CGA
1224 TCGA
Van91I 252 CCAAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1194 CCAGGCATTGG
XcmI 173 CCATGAACAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1608 CCAACCCTCCACTGG
XhoII 807 GGATCT [GA]^GATC[CT]
1421 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AfaI AluI AvaII BbvI BfaI
BmyI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MaeI MboI MboII MnlI MseI
MvaI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfcI SmlI TfiI Tru9I TseI
Tsp509I TspRI