(Circular) (Six-base) MAPSORT of: pFN21AE6429.seq Check: 192 from: 1 to: 6490 pFN21AE6429 6490 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2716 2716 Size: 6490 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4300 278 Size: 53 83 186 936 2764 2468 Fragments arranged by size: 2764 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2876 3833 1937 Size: 939 957 4594 Fragments arranged by size: 4594 957 939 Acc65I G'GTAC_C Cuts at: 3811 3811 Size: 6490 AclI AA'CG_TT Cuts at: 1563 4617 4990 1563 Size: 3054 373 3063 Fragments arranged by size: 3063 3054 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4553 5937 6205 1632 Size: 2921 1384 268 1917 Fragments arranged by size: 2921 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6293 AflIII A'CryG_T Cuts at: 1203 2318 5389 6366 1203 Size: 1115 3071 977 1327 Fragments arranged by size: 3071 1327 1115 977 AgeI A'CCGG_T Cuts at: 5321 5321 Size: 6490 AhdI GACnn_n'nnGTC Cuts at: 2872 5219 2872 Size: 2347 4143 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6490 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6490 AlwNI CAG_nnn'CTG Cuts at: 2043 4233 5805 2043 Size: 2190 1572 2728 Fragments arranged by size: 2728 2190 1572 ApaI G_GGCC'C Cuts at: 2178 2178 Size: 6490 ApaLI G'TGCA_C Cuts at: 4547 5703 4547 Size: 1156 5334 ApoI r'AATT_y Cuts at: 1493 2260 3799 4025 4177 6286 1493 Size: 767 1539 226 152 2109 1697 Fragments arranged by size: 2109 1697 1539 767 226 152 AseI AT'TA_AT Cuts at: 160 1032 5044 160 Size: 872 4012 1606 Fragments arranged by size: 4012 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6490 AvaI C'yCGr_G Cuts at: 1870 1943 2011 3815 1870 Size: 73 68 1804 4545 Fragments arranged by size: 4545 1804 73 68 AvrII C'CTAG_G Cuts at: 3676 3676 Size: 6490 BamHI G'GATC_C Cuts at: 3820 4187 3820 Size: 367 6123 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2547 3811 5266 618 Size: 325 217 627 760 1264 1455 1842 Fragments arranged by size: 1842 1455 1264 760 627 325 217 BanII G_rGCy'C Cuts at: 1391 2012 2178 3809 1391 Size: 621 166 1631 4072 Fragments arranged by size: 4072 1631 621 166 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2898 3759 928 Size: 971 999 861 3659 Fragments arranged by size: 3659 999 971 861 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4681 4715 1903 Size: 34 2744 34 3678 Fragments arranged by size: 3678 2744 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4382 5592 4382 Size: 1210 5280 BclI T'GATC_A Cuts at: 1548 2539 1548 Size: 991 5499 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5101 136 Size: 107 122 71 631 605 3429 1525 Fragments arranged by size: 3429 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3426 6485 3426 Size: 3059 3431 BlpI GC'TnA_GC Cuts at: 3907 3907 Size: 6490 Bme1580I G_kGCm'C Cuts at: 1790 2178 2326 4551 5707 1790 Size: 388 148 2225 1156 2573 Fragments arranged by size: 2573 2225 1156 388 148 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6490 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4221 5179 442 Size: 416 53 83 399 24 2804 958 1753 Fragments arranged by size: 2804 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6490 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3592 3592 Size: 6490 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2115 5150 1239 Size: 876 3035 2579 Fragments arranged by size: 3035 2579 876 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3101 3169 3424 4615 5480 5778 3101 Size: 68 255 1191 865 298 3813 Fragments arranged by size: 3813 1191 865 298 255 68 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5153 882 Size: 617 3654 2219 Fragments arranged by size: 3654 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4281 493 Size: 711 3077 2702 Fragments arranged by size: 3077 2702 711 BsaBI GATnn'nnATC Cuts at: 2817 6484 2817 Size: 3667 2823 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4297 4679 5314 275 Size: 53 83 186 936 2764 382 635 1451 Fragments arranged by size: 2764 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4923 5321 5595 5742 6153 4923 Size: 398 274 147 411 5260 Fragments arranged by size: 5260 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3602 3632 1178 Size: 30 165 30 2199 30 4036 Fragments arranged by size: 4036 2199 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2239 3038 1186 Size: 1053 799 4638 Fragments arranged by size: 4638 1053 799 BseYI C'CCAG_C Cuts at: 2052 2085 2121 5693 6189 2052 Size: 33 36 3572 496 2353 Fragments arranged by size: 3572 2353 496 36 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2213 2592 1163 Size: 573 477 379 5061 Fragments arranged by size: 5061 573 477 379 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3940 4701 5729 1540 Size: 275 186 1939 761 1028 2301 Fragments arranged by size: 2301 1939 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 2229 4551 4636 5707 2012 Size: 217 2322 85 1071 2795 Fragments arranged by size: 2795 2322 1071 217 85 BsmI GAATG_Cn' Cuts at: 3321 4001 4094 3321 Size: 680 93 5717 Fragments arranged by size: 5717 680 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2021 2021 Size: 6490 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2178 2229 2326 3809 4551 4636 Size: 399 222 166 51 97 1483 742 85 Cuts at: 4636 5707 1391 Size: 1071 2174 Fragments arranged by size: 2174 1483 1071 742 399 222 166 97 85 51 BspEI T'CCGG_A Cuts at: 6153 6153 Size: 6490 BspHI T'CATG_A Cuts at: 3514 4379 6109 3514 Size: 865 1730 3895 Fragments arranged by size: 3895 1730 865 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2716 3310 3845 844 Size: 1872 594 535 3489 Fragments arranged by size: 3489 1872 594 535 BsrBI CCG'CTC Cuts at: 1394 1823 4377 1394 Size: 429 2554 3507 Fragments arranged by size: 3507 2554 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4985 5159 65 Size: 1183 3737 174 1396 Fragments arranged by size: 3737 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5134 5321 1700 Size: 110 3324 187 2869 Fragments arranged by size: 3324 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6490 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6490 BssSI C'ACGA_G Cuts at: 4550 5562 4550 Size: 1012 5478 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6490 BstBI TT'CG_AA Cuts at: 5294 5294 Size: 6490 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6490 BstYI r'GATC_y Cuts at: 1972 2812 3426 3820 4187 4572 4589 6041 6127 Size: 840 614 394 367 385 17 1452 86 Cuts at: 6127 6139 6485 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 840 614 394 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2556 2556 Size: 6490 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5938 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5751 Fragments arranged by size: 5751 683 56 BtsI GCAGTG_nn' Cuts at: 2081 2095 2821 4006 4800 4820 2081 Size: 14 726 1185 794 20 3751 Fragments arranged by size: 3751 1185 794 726 20 14 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6490 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6455 DraI TTT'AAA Cuts at: 2307 3794 2307 Size: 1487 5003 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5497 809 Size: 507 4181 1802 Fragments arranged by size: 4181 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3937 4826 6455 Size: 54 1425 50 275 18 2107 889 1629 Cuts at: 6455 8 Size: 43 Fragments arranged by size: 2107 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3937 1537 Size: 275 2125 4090 Fragments arranged by size: 4090 2125 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3231 3531 4420 1353 Size: 1878 300 889 3423 Fragments arranged by size: 3423 1878 889 300 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5077 5451 5597 387 Size: 1064 138 3488 374 146 1280 Fragments arranged by size: 3488 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2010 2010 Size: 6490 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2115 4553 5150 5937 6205 1239 Size: 393 483 2438 597 787 268 1524 Fragments arranged by size: 2438 1524 787 597 483 393 268 EcoO109I rG'GnC_Cy Cuts at: 2163 3441 2163 Size: 1278 5212 EcoRI G'AATT_C Cuts at: 3799 4177 3799 Size: 378 6112 FspI TGC'GCA Cuts at: 4996 4996 Size: 6490 HaeII r_GCGC'y Cuts at: 1678 5637 1678 Size: 3959 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2858 2885 2890 2917 3038 Size: 32 135 32 1286 27 5 27 121 Cuts at: 3038 3070 3652 3684 5131 5163 5205 5237 1373 Size: 32 582 32 1447 32 42 32 2626 Fragments arranged by size: 2626 1447 1286 582 135 121 42 32 32 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 669 1657 1938 3834 4080 669 Size: 988 281 1896 246 3079 Fragments arranged by size: 3079 1896 988 281 246 HindIII A'AGCT_T Cuts at: 748 3509 3526 748 Size: 2761 17 3712 Fragments arranged by size: 3712 2761 17 HpaI GTT'AAC Cuts at: 4080 4080 Size: 6490 KpnI G_GTAC'C Cuts at: 3815 3815 Size: 6490 MfeI C'AATT_G Cuts at: 4089 4089 Size: 6490 MluI A'CGCG_T Cuts at: 6366 6366 Size: 6490 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2324 2399 2608 2868 3013 5604 5788 1305 Size: 1019 75 209 260 145 2591 184 2007 Fragments arranged by size: 2591 2007 1019 260 209 184 145 75 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4668 Fragments arranged by size: 4668 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3389 3465 4448 4807 4966 518 Size: 648 2223 76 983 359 159 2042 Fragments arranged by size: 2223 2042 983 648 359 159 76 MspA1I CmG'CkG Cuts at: 1606 2043 3906 4583 5731 5976 1606 Size: 437 1863 677 1148 245 2120 Fragments arranged by size: 2120 1863 1148 677 437 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5938 NdeI CA'TA_TG Cuts at: 387 387 Size: 6490 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6490 NotI GC'GGCC_GC Cuts at: 3937 3937 Size: 6490 NspI r_CATG'y Cuts at: 1132 1229 2322 3848 5393 1132 Size: 97 1093 1526 1545 2229 Fragments arranged by size: 2229 1545 1526 1093 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6490 PciI A'CATG_T Cuts at: 2318 5389 2318 Size: 3071 3419 PflMI CCAn_nnn'nTGG Cuts at: 1108 2263 3205 1108 Size: 1155 942 4393 Fragments arranged by size: 4393 1155 942 PmeI GTTT'AAAC Cuts at: 3794 3794 Size: 6490 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4559 4591 6072 6104 4559 Size: 32 1481 32 4945 Fragments arranged by size: 4945 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2163 3441 2163 Size: 1278 5212 PshAI GACnn'nnGTC Cuts at: 1613 3439 1613 Size: 1826 4664 PsiI TTA'TAA Cuts at: 4060 4060 Size: 6490 PspOMI G'GGCC_C Cuts at: 2174 2174 Size: 6490 PspXI vC'TCGA_Gb Cuts at: 2011 2011 Size: 6490 PstI C_TGCA'G Cuts at: 830 2076 2961 3434 3842 830 Size: 1246 885 473 408 3478 Fragments arranged by size: 3478 1246 885 473 408 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6490 PvuII CAG'CTG Cuts at: 2043 2043 Size: 6490 SacI G_AGCT'C Cuts at: 2012 2012 Size: 6490 SalI G'TCGA_C Cuts at: 1936 3832 1936 Size: 1896 4594 SbfI CC_TGCA'GG Cuts at: 3842 3842 Size: 6490 ScaI AGT'ACT Cuts at: 2332 4738 6450 2332 Size: 2406 1712 2372 Fragments arranged by size: 2406 2372 1712 SexAI A'CCwGG_T Cuts at: 3712 3712 Size: 6490 SfcI C'TryA_G Cuts at: 826 1045 2072 2167 2877 2957 3430 3838 4973 Size: 219 1027 95 710 80 473 408 1135 Cuts at: 4973 5654 5845 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1027 710 681 473 408 219 191 95 80 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6490 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6490 SmaI CCC'GGG Cuts at: 1872 3817 1872 Size: 1945 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2011 3080 3184 3403 4594 5495 Size: 197 926 68 1069 104 219 1191 901 Cuts at: 5495 5757 820 Size: 262 1553 Fragments arranged by size: 1553 1191 1069 926 901 262 219 197 104 68 SnaBI TAC'GTA Cuts at: 493 4281 493 Size: 3788 2702 SpeI A'CTAG_T Cuts at: 152 2992 152 Size: 2840 3650 SphI G_CATG'C Cuts at: 1132 3848 1132 Size: 2716 3774 SspI AAT'ATT Cuts at: 5 52 3275 4414 5 Size: 47 3223 1139 2081 Fragments arranged by size: 3223 2081 1139 47 StuI AGG'CCT Cuts at: 1517 2198 3681 1517 Size: 681 1483 4326 Fragments arranged by size: 4326 1483 681 StyI C'CwwG_G Cuts at: 513 1065 2199 3676 3929 513 Size: 552 1134 1477 253 3074 Fragments arranged by size: 3074 1477 1134 552 253 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4517 4534 4687 4872 5317 325 Size: 1255 2937 17 153 185 445 1498 Fragments arranged by size: 2937 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2330 2425 3654 Size: 274 80 33 51 1443 353 95 1229 Cuts at: 3654 4736 6448 96 Size: 1082 1712 138 Fragments arranged by size: 1712 1443 1229 1082 353 274 138 95 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2155 2187 6450 6482 2155 Size: 32 4263 32 2163 Fragments arranged by size: 4263 2163 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6490 XbaI T'CTAG_A Cuts at: 3826 3826 Size: 6490 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2185 3620 2185 Size: 1435 5055 XhoI C'TCGA_G Cuts at: 1943 2011 1943 Size: 68 6422 XmaI C'CCGG_G Cuts at: 1870 3815 1870 Size: 1945 4545 XmnI GAAnn'nnTTC Cuts at: 4619 4619 Size: 6490 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4298 276 Size: 53 83 186 936 2764 2468 Fragments arranged by size: 2764 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI