################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI KspI MaeI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AciI 1040 CCGC CCGC(-3/-1) Asp700I 915 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 601 TGGCCA TGG^CCA BclI 177 TGATCA T^GATCA BglII 717 AGATCT A^GATCT BsaBI 1157 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 964 GGTCTC GGTCTC(1/5) BseRI 1012 GAGGAG GAGGAG(10/8) BsgI 146 CTGCAC GTGCAG(16/14) BsmFI 496 GGGAC GGGAC(10/14) BspDI 751 ATCGAT AT^CGAT BspLU11I 832 ACATGT A^CATGT BsrDI 506 GCAATG GCAATG(2/0) BstYI 717 AGATCT [GA]^GATC[CT] ClaI 751 ATCGAT AT^CGAT DraI 568 TTTAAA TTT^AAA DraII 689 AGGTCCT [GA]G^G[ACGT]CC[CT] DraIII 8 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 601 TGGCCA [CT]^GGCC[GA] EcoNI 984 CCTACACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 689 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoT22I 290 ATGCAT ATGCA^T FbaI 177 TGATCA T^GATCA MamI 1157 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 717 AGATCT [GA]^GATC[CT] MluNI 601 TGGCCA TGG^CCA MscI 601 TGGCCA TGG^CCA NcoI 902 CCATGG C^CATGG NdeI 293 CATATG CA^TATG NsiI 290 ATGCAT ATGCA^T NspI 832 ACATGT [GA]CATG^[CT] PpuMI 689 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 831 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1031 CAGCTG CAG^CTG SapI 318 GCTCTTC GCTCTTC(1/4) SmlI 883 CTCAAG C^T[CT][GA]AG SspI 353 AATATT AAT^ATT StuI 992 AGGCCT AGG^CCT XhoII 717 AGATCT [GA]^GATC[CT] XmnI 915 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AfaI 628 GTAC GT^AC 679 GTAC AflIII 832 ACATGT A^C[GA][CT]GT 890 ACACGT AvaII 120 GGTCC G^G[AT]CC 690 GGTCC BsiYI 703 CCATACAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 984 CCTACACAAGG BslI 703 CCATACAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 984 CCTACACAAGG BsmAI 829 GAGAC GTCTC(1/5) 965 GTCTC Bsp1407I 627 TGTACA T^GTACA 678 TGTACA BsrGI 627 TGTACA T^GTACA 678 TGTACA BstXI 686 CCAAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 902 CCATGGGAATGG Cfr13I 120 GGTCC G^G[ACGT]CC 690 GGTCC DsaI 902 CCATGG C^C[GA][CT]GG 960 CCACGG EcoRI 3 GAATTC G^AATTC 271 GAATTC EcoT14I 686 CCAAGG C^C[AT][AT]GG 902 CCATGG HindIII 259 AAGCTT A^AGCTT 766 AAGCTT HinfI 394 GAATC G^A[ACGT]TC 1157 GATTC MaeII 334 ACGT A^CGT 892 ACGT MaeIII 184 GTCAC ^GT[ACGT]AC 1006 GTCAC MslI 726 CATGATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 903 CATGGGAATG MspA1I 1031 CAGCTG C[AC]G^C[GT]G 1040 CCGCTG MwoI 1027 GCTGCAGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1033 GCTGCCACCGC NlaIV 130 GGAGCC GG[ACGT]^[ACGT]CC 956 GGAGCC PstI 760 CTGCAG CTGCA^G 1028 CTGCAG RsaI 628 GTAC GT^AC 679 GTAC Sau96I 120 GGTCC G^G[ACGT]CC 690 GGTCC SspBI 627 TGTACA T^GTACA 678 TGTACA StyI 686 CCAAGG C^C[AT][AT]GG 902 CCATGG TfiI 394 GAATC G^A[AT]TC 1157 GATTC Tsp45I 184 GTCAC ^GT[GC]AC 1006 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AcsI AluI ApoI BbvI BpmI BsaJI BsrI BssKI BstF5I BstNI DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HphI ItaI Ksp632I MboI MboII MnlI MseI MvaI NdeII NlaIII Sau3AI ScrFI SfaNI SfcI TaqI Tru9I TseI Tsp509I TspRI TthHB8I