################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## Acc65I AclI AcsI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII BanI BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BglI BglII BpmI BpuAI BsaBI BsaI BsiWI BsmBI BsmI BsoBI BspDI BspHI BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MseI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 949 GACGTC GACGT^C AccI 310 GTCTAC GT^[AC][GT]AC AcyI 949 GACGTC G[GA]^CG[CT]C AflIII 566 ACATGT A^C[GA][CT]GT ApaI 360 GGGCCC GGGCC^C AvrII 1143 CCTAGG C^CTAGG BalI 275 TGGCCA TGG^CCA BamHI 1011 GGATCC G^GATCC BcgI 1031 CGACTCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 1143 CCTAGG C^CTAGG BlpI 1125 GCTTAGC GC^T[ACGT]AGC Bpu1102I 1125 GCTTAGC GC^T[ACGT]AGC BsaAI 610 TACGTG [CT]AC^GT[GA] BsaHI 949 GACGTC G[GA]^CG[CT]C BsmAI 495 GTCTC GTCTC(1/5) Bsp1407I 569 TGTACA T^GTACA BspLU11I 566 ACATGT A^CATGT BsrGI 569 TGTACA T^GTACA BstAPI 1160 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 1125 GCTTAGC GC^T[ACGT]AGC DsaI 558 CCACGG C^C[GA][CT]GG EagI 868 CGGCCG C^GGCCG EclXI 868 CGGCCG C^GGCCG Eco52I 868 CGGCCG C^GGCCG EcoT14I 1143 CCTAGG C^C[AT][AT]GG HgaI 340 GCGTC GACGC(5/10) Hin1I 949 GACGTC G[GA]^CG[CT]C HindIII 874 AAGCTT A^AGCTT MluNI 275 TGGCCA TGG^CCA MscI 275 TGGCCA TGG^CCA MspA1I 691 CAGCTG C[AC]G^C[GT]G PleI 1032 GACTC GAGTC(4/5) PpuMI 685 GGGACCC [GA]G^G[AT]CC[CT] PvuII 691 CAGCTG CAG^CTG SacI 553 GAGCTC GAGCT^C SexAI 167 ACCTGGT A^CC[AT]GGT SspBI 569 TGTACA T^GTACA StyI 1143 CCTAGG C^C[AT][AT]GG TaqI 623 TCGA T^CGA TfiI 326 GAATC G^A[AT]TC TthHB8I 623 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccIII 50 TCCGGA T^CCGGA 287 TCCGGA Alw44I 709 GTGCAC G^TGCAC 1118 GTGCAC AocI 105 CCTGAGG CC^T[ACGT]AGG 519 CCTGAGG ApaLI 709 GTGCAC G^TGCAC 1118 GTGCAC BsaWI 50 TCCGGA [AT]^CCGG[AT] 287 TCCGGA BseAI 50 TCCGGA T^CCGGA 287 TCCGGA BsiEI 761 CGACCG CG[GA][CT]^CG 868 CGGCCG BspEI 50 TCCGGA T^CCGGA 287 TCCGGA BsrBI 21 CCGCTC CCGCTC(-3/-3) 43 GAGCGG BstYI 986 AGATCC [GA]^GATC[CT] 1011 GGATCC Bsu36I 105 CCTGAGG CC^T[ACGT]AGG 519 CCTGAGG DpnII 987 GATC ^GATC 1012 GATC Eco81I 105 CCTGAGG CC^T[ACGT]AGG 519 CCTGAGG HinfI 326 GAATC G^A[ACGT]TC 1032 GACTC MboI 987 GATC ^GATC 1012 GATC MboII 1051 GAAGA GAAGA(8/7) 1188 GAAGA MflI 986 AGATCC [GA]^GATC[CT] 1011 GGATCC MroI 50 TCCGGA T^CCGGA 287 TCCGGA NdeII 987 GATC ^GATC 1012 GATC NspI 486 GCATGT [GA]CATG^[CT] 566 ACATGT PstI 181 CTGCAG CTGCA^G 892 CTGCAG Sau3AI 987 GATC ^GATC 1012 GATC SfaNI 101 GCATC GCATC(5/9) 452 GCATC XcmI 516 CCACCTGAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1016 CCATTTTCTCACTGG XhoII 986 AGATCC [GA]^GATC[CT] 1011 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaII BanII BbvI BcnI BfaI BmyI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DraII EaeI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeI MaeII MaeIII MnlI MslI MspI MvaI MvnI MwoI NaeI NciI NgoMIV NlaIII NlaIV RsaI Sau96I ScrFI SfcI TseI Tsp45I TspRI