(Circular) (Six-base) MAPSORT of: pFN21AE0497.seq Check: 6687 from: 1 to: 5905 pFN21AE0497 5905 bp With 182 enzymes: SgfI * February 2, 2010 11:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2997 2997 Size: 5905 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2958 3715 278 Size: 53 83 186 936 1422 757 2468 Fragments arranged by size: 2468 1422 936 757 186 83 53 AccI GT'mk_AC Cuts at: 1937 2316 3248 1937 Size: 379 932 4594 Fragments arranged by size: 4594 932 379 Acc65I G'GTAC_C Cuts at: 3226 3226 Size: 5905 AclI AA'CG_TT Cuts at: 1563 4032 4405 1563 Size: 2469 373 3063 Fragments arranged by size: 3063 2469 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2237 2375 2837 2951 3968 5352 5620 1632 Size: 605 138 462 114 1017 1384 268 1917 Fragments arranged by size: 1917 1384 1017 605 462 268 138 114 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5708 AflIII A'CryG_T Cuts at: 1203 2571 4804 5781 1203 Size: 1368 2233 977 1327 Fragments arranged by size: 2233 1368 1327 977 AgeI A'CCGG_T Cuts at: 4736 4736 Size: 5905 AhdI GACnn_n'nnGTC Cuts at: 4634 4634 Size: 5905 AleI CACnn'nnGTG Cuts at: 1166 2685 2711 1166 Size: 1519 26 4360 Fragments arranged by size: 4360 1519 26 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5905 AlwNI CAG_nnn'CTG Cuts at: 2482 2749 3171 3648 5220 2482 Size: 267 422 477 1572 3167 Fragments arranged by size: 3167 1572 477 422 267 ApaI G_GGCC'C Cuts at: 2369 2369 Size: 5905 ApaLI G'TGCA_C Cuts at: 2714 3123 3962 5118 2714 Size: 409 839 1156 3501 Fragments arranged by size: 3501 1156 839 409 ApoI r'AATT_y Cuts at: 1493 3214 3440 3592 5701 1493 Size: 1721 226 152 2109 1697 Fragments arranged by size: 2109 1721 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4459 160 Size: 872 3427 1606 Fragments arranged by size: 3427 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 AvaI C'yCGr_G Cuts at: 1870 1943 3230 1870 Size: 73 1287 4545 Fragments arranged by size: 4545 1287 73 AvrII C'CTAG_G Cuts at: 3148 3148 Size: 5905 BamHI G'GATC_C Cuts at: 3016 3235 3602 3016 Size: 219 367 5319 Fragments arranged by size: 5319 367 219 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3226 4681 618 Size: 325 217 627 1439 1455 1842 Fragments arranged by size: 1842 1455 1439 627 325 217 BanII G_rGCy'C Cuts at: 1391 2369 2520 2562 3224 1391 Size: 978 151 42 662 4072 Fragments arranged by size: 4072 978 662 151 42 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4934 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3025 3059 4096 4130 1903 Size: 34 1088 34 1037 34 3678 Fragments arranged by size: 3678 1088 1037 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3797 5007 3797 Size: 1210 4695 BclI T'GATC_A Cuts at: 1548 1548 Size: 5905 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4516 136 Size: 107 122 71 631 605 2844 1525 Fragments arranged by size: 2844 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5900 5900 Size: 5905 BlpI GC'TnA_GC Cuts at: 3131 3322 3131 Size: 191 5714 Bme1580I G_kGCm'C Cuts at: 1790 2369 2718 3127 3966 5122 1790 Size: 579 349 409 839 1156 2573 Fragments arranged by size: 2573 1156 839 579 409 349 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5905 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2764 2929 3636 Size: 416 53 83 399 24 1347 165 707 Cuts at: 3636 4594 442 Size: 958 1753 Fragments arranged by size: 1753 1347 958 707 416 399 165 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5905 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4565 1239 Size: 3326 2579 Bpu10I CC'TnA_GC Cuts at: 2423 2423 Size: 5905 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4030 4895 5193 4030 Size: 865 298 4742 Fragments arranged by size: 4742 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4568 882 Size: 617 3069 2219 Fragments arranged by size: 3069 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2617 3696 493 Size: 711 1413 1079 2702 Fragments arranged by size: 2702 1413 1079 711 BsaBI GATnn'nnATC Cuts at: 5899 5899 Size: 5905 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2955 3712 4094 4729 Size: 53 83 186 936 1422 757 382 635 Cuts at: 4729 275 Size: 1451 Fragments arranged by size: 1451 1422 936 757 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2055 2292 4338 4736 5010 5157 5568 2055 Size: 237 2046 398 274 147 411 2392 Fragments arranged by size: 2392 2046 411 398 274 237 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2222 2252 1178 Size: 30 165 30 819 30 4831 Fragments arranged by size: 4831 819 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2084 2156 2246 2267 2542 2957 3113 1186 Size: 898 72 90 21 275 415 156 3978 Fragments arranged by size: 3978 898 415 275 156 90 72 21 BseYI C'CCAG_C Cuts at: 2694 2980 5108 5604 2694 Size: 286 2128 496 2995 Fragments arranged by size: 2995 2128 496 286 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2507 3106 3157 1163 Size: 573 771 599 51 3911 Fragments arranged by size: 3911 771 599 573 51 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2769 2876 3355 4116 5144 1540 Size: 275 186 768 107 479 761 1028 2301 Fragments arranged by size: 2301 1028 768 761 479 275 186 107 BsiHKAI G_wGCw'C Cuts at: 2562 2718 3127 3966 4051 5122 2562 Size: 156 409 839 85 1071 3345 Fragments arranged by size: 3345 1071 839 409 156 85 BsmI GAATG_Cn' Cuts at: 3416 3509 3416 Size: 93 5812 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2369 2520 2562 2718 3127 3224 3966 Size: 399 579 151 42 156 409 97 742 Cuts at: 3966 4051 5122 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 579 409 399 156 151 97 85 42 BspEI T'CCGG_A Cuts at: 2055 2292 5568 2055 Size: 237 3276 2392 Fragments arranged by size: 3276 2392 237 BspHI T'CATG_A Cuts at: 3794 5524 3794 Size: 1730 4175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2684 2904 2997 3260 844 Size: 1840 220 93 263 3489 Fragments arranged by size: 3489 1840 263 220 93 BsrBI CCG'CTC Cuts at: 1394 1823 2028 2050 3792 1394 Size: 429 205 22 1742 3507 Fragments arranged by size: 3507 1742 429 205 22 BsrDI GCAATG_nn' Cuts at: 65 1248 4400 4574 65 Size: 1183 3152 174 1396 Fragments arranged by size: 3152 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2148 2199 2868 4549 4736 1700 Size: 110 338 51 669 1681 187 2869 Fragments arranged by size: 2869 1681 669 338 187 110 51 BsrGI T'GTAC_A Cuts at: 96 2574 96 Size: 2478 3427 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5905 BssSI C'ACGA_G Cuts at: 3965 4977 3965 Size: 1012 4893 BstAPI GCAn_nnn'nTGC Cuts at: 794 3171 794 Size: 2377 3528 BstBI TT'CG_AA Cuts at: 4709 4709 Size: 5905 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5905 BstXI CCAn_nnnn'nTGG Cuts at: 2308 2687 2844 2308 Size: 379 157 5369 Fragments arranged by size: 5369 379 157 BstYI r'GATC_y Cuts at: 1972 2991 3016 3235 3602 3987 4004 5456 5542 Size: 1019 25 219 367 385 17 1452 86 Cuts at: 5542 5554 5900 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1019 385 367 346 219 86 25 17 12 Bsu36I CC'TnA_GG Cuts at: 2111 2525 2111 Size: 414 5491 BtgI C'CryG_G Cuts at: 513 1065 2004 2563 513 Size: 552 939 559 3855 Fragments arranged by size: 3855 939 559 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5166 Fragments arranged by size: 5166 683 56 BtsI GCAGTG_nn' Cuts at: 2384 2643 2906 3421 4215 4235 2384 Size: 259 263 515 794 20 4054 Fragments arranged by size: 4054 794 515 263 259 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5905 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5870 DraI TTT'AAA Cuts at: 3209 3209 Size: 5905 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4912 809 Size: 507 3596 1802 Fragments arranged by size: 3596 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2157 2280 2547 Size: 54 1425 50 275 18 327 123 267 Cuts at: 2547 2873 2973 3352 4241 5870 8 Size: 326 100 379 889 1629 43 Fragments arranged by size: 1629 1425 889 379 327 326 275 267 123 100 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2873 3352 1537 Size: 275 1061 479 4090 Fragments arranged by size: 4090 1061 479 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3835 1353 Size: 2482 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2060 2081 2141 4492 4866 5012 Size: 1064 138 471 21 60 2351 374 146 Cuts at: 5012 387 Size: 1280 Fragments arranged by size: 2351 1280 1064 471 374 146 138 60 21 EcoICRI GAG'CTC Cuts at: 2560 2560 Size: 5905 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2237 2375 2837 2951 3968 4565 5352 Size: 393 605 138 462 114 1017 597 787 Cuts at: 5352 5620 1239 Size: 268 1524 Fragments arranged by size: 1524 1017 787 605 597 462 393 268 138 114 EcoNI CCTnn'n_nnAGG Cuts at: 2093 2114 2426 3004 2093 Size: 21 312 578 4994 Fragments arranged by size: 4994 578 312 21 EcoO109I rG'GnC_Cy Cuts at: 2212 2365 2366 2691 3103 2212 Size: 153 1 325 412 5014 Fragments arranged by size: 5014 412 325 153 1 EcoRI G'AATT_C Cuts at: 3214 3592 3214 Size: 378 5527 FspI TGC'GCA Cuts at: 4411 4411 Size: 5905 HaeII r_GCGC'y Cuts at: 1678 2089 2139 2289 5052 1678 Size: 411 50 150 2763 2531 Fragments arranged by size: 2763 2531 411 150 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4546 4578 4620 4652 1373 Size: 32 135 32 2974 32 42 32 2626 Fragments arranged by size: 2974 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3249 3495 669 Size: 988 281 1311 246 3079 Fragments arranged by size: 3079 1311 988 281 246 HindIII A'AGCT_T Cuts at: 748 2879 748 Size: 2131 3774 HpaI GTT'AAC Cuts at: 3495 3495 Size: 5905 KpnI G_GTAC'C Cuts at: 3230 3230 Size: 5905 MfeI C'AATT_G Cuts at: 3504 3504 Size: 5905 MluI A'CGCG_T Cuts at: 5781 5781 Size: 5905 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3053 5019 5203 1305 Size: 1748 1966 184 2007 Fragments arranged by size: 2007 1966 1748 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2282 10 Size: 54 1425 343 450 3633 Fragments arranged by size: 3633 1425 450 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2450 2685 2711 3863 4222 4381 518 Size: 648 1284 235 26 1152 359 159 2042 Fragments arranged by size: 2042 1284 1152 648 359 235 159 26 MspA1I CmG'CkG Cuts at: 1606 2698 3321 3998 5146 5391 1606 Size: 1092 623 677 1148 245 2120 Fragments arranged by size: 2120 1148 1092 677 623 245 NaeI GCC'GGC Cuts at: 2150 2201 2870 2150 Size: 51 669 5185 Fragments arranged by size: 5185 669 51 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4414 Fragments arranged by size: 4414 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5905 NgoMIV G'CCGG_C Cuts at: 2148 2199 2868 2148 Size: 51 669 5185 Fragments arranged by size: 5185 669 51 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5905 NotI GC'GGCC_GC Cuts at: 3352 3352 Size: 5905 NspI r_CATG'y Cuts at: 1132 1229 2495 2575 3263 4808 1132 Size: 97 1266 80 688 1545 2229 Fragments arranged by size: 2229 1545 1266 688 97 80 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5905 PciI A'CATG_T Cuts at: 2571 4804 2571 Size: 2233 3672 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5905 PfoI T'CCnGG_A Cuts at: 2271 2271 Size: 5905 PmeI GTTT'AAAC Cuts at: 3209 3209 Size: 5905 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3974 4006 5487 5519 3974 Size: 32 1481 32 4360 Fragments arranged by size: 4360 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2691 2691 Size: 5905 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5905 PsiI TTA'TAA Cuts at: 3475 3475 Size: 5905 PspOMI G'GGCC_C Cuts at: 2365 2365 Size: 5905 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2288 2320 2288 Size: 32 5873 PstI C_TGCA'G Cuts at: 830 2190 2901 3257 830 Size: 1360 711 356 3478 Fragments arranged by size: 3478 1360 711 356 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5905 PvuII CAG'CTG Cuts at: 2698 2698 Size: 5905 SacI G_AGCT'C Cuts at: 2562 2562 Size: 5905 SalI G'TCGA_C Cuts at: 1936 3247 1936 Size: 1311 4594 SanDI GG'GwC_CC Cuts at: 2691 2691 Size: 5905 SbfI CC_TGCA'GG Cuts at: 3257 3257 Size: 5905 ScaI AGT'ACT Cuts at: 4153 5865 4153 Size: 1712 4193 SexAI A'CCwGG_T Cuts at: 2172 2172 Size: 5905 SfcI C'TryA_G Cuts at: 826 1045 2186 2897 3001 3253 4388 5069 5260 Size: 219 1141 711 104 252 1135 681 191 Cuts at: 5260 826 Size: 1471 Fragments arranged by size: 1471 1141 1135 711 681 252 219 191 104 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5905 SmaI CCC'GGG Cuts at: 1872 3232 1872 Size: 1360 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4009 4910 5172 820 Size: 197 926 2066 901 262 1553 Fragments arranged by size: 2066 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3696 493 Size: 3203 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5905 SphI G_CATG'C Cuts at: 1132 3263 1132 Size: 2131 3774 SspI AAT'ATT Cuts at: 5 52 3829 5 Size: 47 3777 2081 Fragments arranged by size: 3777 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5905 StyI C'CwwG_G Cuts at: 513 1065 2004 3148 3344 513 Size: 552 939 1144 196 3074 Fragments arranged by size: 3074 1144 939 552 196 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2462 2783 3932 3949 4102 4287 4732 Size: 1255 882 321 1149 17 153 185 445 Cuts at: 4732 325 Size: 1498 Fragments arranged by size: 1498 1255 1149 882 445 321 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2574 4151 5863 Size: 274 80 33 51 1443 597 1577 1712 Cuts at: 5863 96 Size: 138 Fragments arranged by size: 1712 1577 1443 597 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2222 2254 5865 5897 2222 Size: 32 3611 32 2230 Fragments arranged by size: 3611 2230 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5905 XbaI T'CTAG_A Cuts at: 3241 3241 Size: 5905 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2528 3028 2528 Size: 500 5405 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5905 XmaI C'CCGG_G Cuts at: 1870 3230 1870 Size: 1360 4545 XmnI GAAnn'nnTTC Cuts at: 4034 4034 Size: 5905 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2956 3713 276 Size: 53 83 186 936 1422 757 2468 Fragments arranged by size: 2468 1422 936 757 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI RsrII SacII SapI SfiI SfoI SrfI SwaI