################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvrII BamHI BbrPI BbvCI BcgI BciVI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmI Bsp1407I BspEI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraIII EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FseI HindIII HpaI KpnI Ksp632I KspI MaeII MamI MfeI MluI MroI MseI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 562 GTCTAC GT^[AC][GT]AC AcsI 274 AAATTT [GA]^AATT[CT] AhdI 778 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 740 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 219 GGGCCC GGGCC^C ApoI 274 AAATTT [GA]^AATT[CT] AspEI 778 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 545 TGCGCA TGC^GCA BclI 455 TGATCA T^GATCA BfaI 289 CTAG C^TAG BglI 688 GCCATCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 405 AGATCT A^GATCT BsaI 867 GGTCTC GGTCTC(1/5) BseRI 1240 GAGGAG GAGGAG(10/8) BsmBI 149 GAGACG CGTCTC(1/5) BspDI 1056 ATCGAT AT^CGAT BspMI 68 ACCTGC ACCTGC(4/8) BstAPI 1261 GCAATCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 405 AGATCT [GA]^GATC[CT] ClaI 1056 ATCGAT AT^CGAT DrdI 955 GACTTGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 778 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 455 TGATCA T^GATCA FspI 545 TGCGCA TGC^GCA HapII 24 CCGG C^CGG HpaII 24 CCGG C^CGG MaeI 289 CTAG C^TAG MflI 405 AGATCT [GA]^GATC[CT] MspI 24 CCGG C^CGG PflMI 939 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 380 GGGTCCC [GA]G^G[AT]CC[CT] ScaI 211 AGTACT AGT^ACT SmaI 23 CCCGGG CCC^GGG SmlI 574 CTCAAG C^T[CT][GA]AG StuI 1004 AGGCCT AGG^CCT Tsp509I 275 AATT ^AATT Van91I 939 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 405 AGATCT [GA]^GATC[CT] XmaCI 23 CCCGGG C^CCGGG XmaI 23 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AcyI 553 GGCGCC G[GA]^CG[CT]C 1186 GGCGCC AvaI 23 CCCGGG C^[CT]CG[GA]G 924 CTCGGG BanI 553 GGCGCC G^G[CT][GA]CC 1186 GGCGCC BbeI 553 GGCGCC GGCGC^C 1186 GGCGCC BbsI 681 GAAGAC GAAGAC(2/6) 877 GAAGAC BcnI 23 CCCGG CC^[GC]GG 24 CCGGG BpuAI 681 GAAGAC GAAGAC(2/6) 877 GAAGAC BsaHI 553 GGCGCC G[GA]^CG[CT]C 1186 GGCGCC BsgI 70 CTGCAC GTGCAG(16/14) 1164 CTGCAC BsoBI 23 CCCGGG C^[CT]CG[GA]G 924 CTCGGG BspHI 503 TCATGA T^CATGA 1229 TCATGA BsrBI 1113 CCGCTC CCGCTC(-3/-3) 1197 CCGCTC BsrDI 1140 CATTGC GCAATG(2/0) 758 GCAATG BstEII 703 GGTTACC G^GT[ACGT]ACC 1047 GGTGACC BstPI 703 GGTTACC G^GT[ACGT]ACC 1047 GGTGACC DsaI 53 CCACGG C^C[GA][CT]GG 119 CCACGG EcoO65I 703 GGTTACC G^GT[ACGT]ACC 1047 GGTGACC EcoT14I 80 CCAAGG C^C[AT][AT]GG 344 CCTTGG FauI 799 CCCGC CCCGC(4/6) 361 GCGGG HaeII 553 GGCGCC [GA]GCGC^[CT] 1186 GGCGCC HgaI 1274 GACGC GACGC(5/10) 1206 GCGTC Hin1I 553 GGCGCC G[GA]^CG[CT]C 1186 GGCGCC HincII 268 GTTGAC GT[CT]^[GA]AC 1030 GTCAAC HindII 268 GTTGAC GT[CT]^[GA]AC 1030 GTCAAC KasI 553 GGCGCC G^GCGCC 1186 GGCGCC MslI 612 CACCAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 729 CATGGATGTG MspA1I 98 CAGCTG C[AC]G^C[GT]G 368 CAGCTG NarI 553 GGCGCC GG^CGCC 1186 GGCGCC NciI 23 CCCGG CC^[GC]GG 24 CCGGG PleI 37 GACTC GAGTC(4/5) 182 GAGTC PvuII 98 CAGCTG CAG^CTG 368 CAGCTG RcaI 503 TCATGA T^CATGA 1229 TCATGA SfaNI 607 GATGC GCATC(5/9) 742 GCATC SfoI 553 GGCGCC GGC^GCC 1186 GGCGCC StyI 80 CCAAGG C^C[AT][AT]GG 344 CCTTGG Tsp45I 964 GTCAC ^GT[GC]AC 1048 GTGAC XcmI 53 CCACGGTGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 323 CCAATGCCATCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BalI BanII BbvI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI MaeIII MboI MboII MluNI MnlI MscI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TaqI TfiI TseI TspRI TthHB8I