Restriction map for
pFN21AE5510
(Circular) (Six-base) MAPSORT of: pFN21AE5510.seq Check: 243 from: 1 to: 6007
pFN21AE5510 6007 bp
With 182 enzymes: SgfI *
December 8, 2014 13:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3817 278
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2568 3350 1937
Size: 631 782 4594
Fragments arranged by size:
4594 782 631
Acc65I G'GTAC_C
Cuts at: 3328 3328
Size: 6007
AclI AA'CG_TT
Cuts at: 1563 4134 4507 1563
Size: 2571 373 3063
Fragments arranged by size:
3063 2571 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2213 2705 3050 4070 5454 5722 1632
Size: 581 492 345 1020 1384 268 1917
Fragments arranged by size:
1917 1384 1020 581 492 345 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5810
AflIII A'CryG_T
Cuts at: 1203 4906 5883 1203
Size: 3703 977 1327
Fragments arranged by size:
3703 1327 977
AgeI A'CCGG_T
Cuts at: 4838 4838
Size: 6007
AhdI GACnn_n'nnGTC
Cuts at: 2788 4736 2788
Size: 1948 4059
AleI CACnn'nnGTG
Cuts at: 1166 2621 1166
Size: 1455 4552
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3038 1184
Size: 1854 4153
AlwNI CAG_nnn'CTG
Cuts at: 2750 3750 5322 2750
Size: 1000 1572 3435
Fragments arranged by size:
3435 1572 1000
ApaI G_GGCC'C
Cuts at: 2228 2228
Size: 6007
ApaLI G'TGCA_C
Cuts at: 4064 5220 4064
Size: 1156 4851
ApoI r'AATT_y
Cuts at: 1493 2279 3316 3542 3694 5803 1493
Size: 786 1037 226 152 2109 1697
Fragments arranged by size:
2109 1697 1037 786 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4561 160
Size: 872 3529 1606
Fragments arranged by size:
3529 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
AvaI C'yCGr_G
Cuts at: 1870 1943 2028 2929 3332 1870
Size: 73 85 901 403 4545
Fragments arranged by size:
4545 901 403 85 73
BamHI G'GATC_C
Cuts at: 3337 3704 3337
Size: 367 5640
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2558 3191 3328 4783 618
Size: 325 217 627 771 633 137 1455 1842
Fragments arranged by size:
1842 1455 771 633 627 325 217 137
BanII G_rGCy'C
Cuts at: 1391 2228 2474 3168 3326 1391
Size: 837 246 694 158 4072
Fragments arranged by size:
4072 837 694 246 158
BbeI G_GCGC'C
Cuts at: 2562 3195 2562
Size: 633 5374
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2693 2889 928
Size: 971 794 196 4046
Fragments arranged by size:
4046 971 794 196
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4198 4232 1903
Size: 34 2261 34 3678
Fragments arranged by size:
3678 2261 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3899 5109 3899
Size: 1210 4797
BclI T'GATC_A
Cuts at: 1548 2460 1548
Size: 912 5095
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2699 4618 136
Size: 107 122 71 631 605 1027 1919 1525
Fragments arranged by size:
1919 1525 1027 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2410 6002 2410
Size: 3592 2415
BlpI GC'TnA_GC
Cuts at: 3424 3424
Size: 6007
Bme1580I G_kGCm'C
Cuts at: 1790 2112 2228 4068 5224 1790
Size: 322 116 1840 1156 2573
Fragments arranged by size:
2573 1840 1156 322 116
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6007
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2106 2435 2829
Size: 416 53 83 399 24 689 329 394
Cuts at: 2829 2914 3738 4696 442
Size: 85 824 958 1753
Fragments arranged by size:
1753 958 824 689 416 399 394 329
85 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2029 2678 2696 4667 1239
Size: 790 649 18 1971 2579
Fragments arranged by size:
2579 1971 790 649 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2564 4132 4997 5295 2564
Size: 1568 865 298 3276
Fragments arranged by size:
3276 1568 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2878 4670 882
Size: 617 1379 1792 2219
Fragments arranged by size:
2219 1792 1379 617
BsaAI yAC'GTr
Cuts at: 493 1204 3798 493
Size: 711 2594 2702
Fragments arranged by size:
2702 2594 711
BsaBI GATnn'nnATC
Cuts at: 6001 6001
Size: 6007
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2559 3192 3814 4196
Size: 53 83 186 936 1026 633 622 382
Cuts at: 4196 4831 275
Size: 635 1451
Fragments arranged by size:
1451 1026 936 635 633 622 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4440 4838 5112 5259 5670 4440
Size: 398 274 147 411 4777
Fragments arranged by size:
4777 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2456 2486 1178
Size: 30 165 30 1053 30 4699
Fragments arranged by size:
4699 1053 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3260 1186
Size: 2074 3933
BseYI C'CCAG_C
Cuts at: 2417 3096 5210 5706 2417
Size: 679 2114 496 2718
Fragments arranged by size:
2718 2114 679 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2060 3154 1163
Size: 573 324 1094 4016
Fragments arranged by size:
4016 1094 573 324
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3457 4218 5246 1540
Size: 275 186 1456 761 1028 2301
Fragments arranged by size:
2301 1456 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4068 4153 5224 4068
Size: 85 1071 4851
Fragments arranged by size:
4851 1071 85
BsmI GAATG_Cn'
Cuts at: 3518 3611 3518
Size: 93 5914
BsmBI CGTCTCn'nnnn_
Cuts at: 2148 2148
Size: 6007
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 2228 2474 3168 3326 4068 4153
Size: 399 322 116 246 694 158 742 85
Cuts at: 4153 5224 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 694 399 322 246 158
116 85
BspEI T'CCGG_A
Cuts at: 5670 5670
Size: 6007
BspHI T'CATG_A
Cuts at: 2508 3234 3896 5626 2508
Size: 726 662 1730 2889
Fragments arranged by size:
2889 1730 726 662
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2082 3362 844
Size: 1238 1280 3489
Fragments arranged by size:
3489 1280 1238
BsrBI CCG'CTC
Cuts at: 1394 1823 3120 3204 3894 1394
Size: 429 1297 84 690 3507
Fragments arranged by size:
3507 1297 690 429 84
BsrDI GCAATG_nn'
Cuts at: 65 1248 2770 3144 4502 4676 65
Size: 1183 1522 374 1358 174 1396
Fragments arranged by size:
1522 1396 1358 1183 374 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4651 4838 1700
Size: 110 2841 187 2869
Fragments arranged by size:
2869 2841 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6007
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6007
BssSI C'ACGA_G
Cuts at: 4067 5079 4067
Size: 1012 4995
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3272 794
Size: 2478 3529
BstBI TT'CG_AA
Cuts at: 4811 4811
Size: 6007
BstEII G'GTnAC_C
Cuts at: 1184 2708 3052 1184
Size: 1524 344 4139
Fragments arranged by size:
4139 1524 344
BstXI CCAn_nnnn'nTGG
Cuts at: 2623 2656 2705 3122 2623
Size: 33 49 417 5508
Fragments arranged by size:
5508 417 49 33
BstYI r'GATC_y
Cuts at: 1972 2410 3337 3704 4089 4106 5558 5644 5656
Size: 438 927 367 385 17 1452 86 12
Cuts at: 5656 6002 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 927 438 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2058 2124 513
Size: 552 939 54 66 4396
Fragments arranged by size:
4396 939 552 66 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2389 487
Size: 683 56 1163 4105
Fragments arranged by size:
4105 1163 683 56
BtsI GCAGTG_nn'
Cuts at: 3523 4317 4337 3523
Size: 794 20 5193
Fragments arranged by size:
5193 794 20
ClaI AT'CG_AT
Cuts at: 1554 3062 1554
Size: 1508 4499
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5972
DraI TTT'AAA
Cuts at: 3311 3311
Size: 6007
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2966 5014 809
Size: 507 1650 2048 1802
Fragments arranged by size:
2048 1802 1650 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2082 2352
Size: 54 1425 50 275 18 177 75 270
Cuts at: 2352 2493 2691 3216 3454 4343 5972 8
Size: 141 198 525 238 889 1629 43
Fragments arranged by size:
1629 1425 889 525 275 270 238 198
177 141 75 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3454 1537
Size: 275 1642 4090
Fragments arranged by size:
4090 1642 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3937 1353
Size: 2584 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4594 4968 5114 387
Size: 1064 138 3005 374 146 1280
Fragments arranged by size:
3005 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2029 2213 2678 2696 2705 3050 4070
Size: 393 397 184 465 18 9 345 1020
Cuts at: 4070 4667 5454 5722 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1020 787 597 465 397 393 345
268 184 18 9
EcoO109I rG'GnC_Cy
Cuts at: 2225 2386 2671 2225
Size: 161 285 5561
Fragments arranged by size:
5561 285 161
EcoRI G'AATT_C
Cuts at: 3316 3694 3316
Size: 378 5629
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3286 3286
Size: 6007
FspI TGC'GCA
Cuts at: 2552 4513 2552
Size: 1961 4046
FspAI rTGC'GCAy
Cuts at: 2552 2552
Size: 6007
HaeII r_GCGC'y
Cuts at: 1678 2562 3195 5154 1678
Size: 884 633 1959 2531
Fragments arranged by size:
2531 1959 884 633
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2297 2329 2774 2806 4648
Size: 32 135 32 725 32 445 32 1842
Cuts at: 4648 4680 4722 4754 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1842 725 445 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2275 3037 3351 3597 669
Size: 988 281 337 762 314 246 3079
Fragments arranged by size:
3079 988 762 337 314 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6007
HpaI GTT'AAC
Cuts at: 3597 3597
Size: 6007
KasI G'GCGC_C
Cuts at: 2558 3191 2558
Size: 633 5374
KpnI G_GTAC'C
Cuts at: 3332 3332
Size: 6007
MfeI C'AATT_G
Cuts at: 3606 3606
Size: 6007
MluI A'CGCG_T
Cuts at: 5883 5883
Size: 6007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5121 5305 1305
Size: 3816 184 2007
Fragments arranged by size:
3816 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2495 3218 10
Size: 54 1425 343 177 486 723 2799
Fragments arranged by size:
2799 1425 723 486 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2621 2738 3965 4324 4483 518
Size: 648 843 612 117 1227 359 159 2042
Fragments arranged by size:
2042 1227 843 648 612 359 159 117
MspA1I CmG'CkG
Cuts at: 1606 2105 2375 3423 4100 5248 5493 1606
Size: 499 270 1048 677 1148 245 2120
Fragments arranged by size:
2120 1148 1048 677 499 270 245
NarI GG'CG_CC
Cuts at: 2559 3192 2559
Size: 633 5374
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4516
Fragments arranged by size:
4516 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6007
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6007
NotI GC'GGCC_GC
Cuts at: 3454 3454
Size: 6007
NspI r_CATG'y
Cuts at: 1132 1229 3365 4910 1132
Size: 97 2136 1545 2229
Fragments arranged by size:
2229 2136 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6007
PciI A'CATG_T
Cuts at: 4906 4906
Size: 6007
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2950 1108
Size: 1842 4165
PfoI T'CCnGG_A
Cuts at: 2655 2655
Size: 6007
PmeI GTTT'AAAC
Cuts at: 3311 3311
Size: 6007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4076 4108 5589 5621 4076
Size: 32 1481 32 4462
Fragments arranged by size:
4462 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2386 2386
Size: 6007
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6007
PsiI TTA'TAA
Cuts at: 3577 3577
Size: 6007
PspOMI G'GGCC_C
Cuts at: 2224 2224
Size: 6007
PstI C_TGCA'G
Cuts at: 830 3359 830
Size: 2529 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6007
PvuII CAG'CTG
Cuts at: 2105 2375 2105
Size: 270 5737
SalI G'TCGA_C
Cuts at: 1936 3349 1936
Size: 1413 4594
SanDI GG'GwC_CC
Cuts at: 2386 2386
Size: 6007
SbfI CC_TGCA'GG
Cuts at: 3359 3359
Size: 6007
ScaI AGT'ACT
Cuts at: 2218 4255 5967 2218
Size: 2037 1712 2258
Fragments arranged by size:
2258 2037 1712
SfcI C'TryA_G
Cuts at: 826 1045 3355 4490 5171 5362 826
Size: 219 2310 1135 681 191 1471
Fragments arranged by size:
2310 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2560 3193 2560
Size: 633 5374
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6007
SmaI CCC'GGG
Cuts at: 1872 2030 3334 1872
Size: 158 1304 4545
Fragments arranged by size:
4545 1304 158
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2579 4111 5012 5274 820
Size: 197 926 636 1532 901 262 1553
Fragments arranged by size:
1553 1532 926 901 636 262 197
SnaBI TAC'GTA
Cuts at: 493 3798 493
Size: 3305 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6007
SphI G_CATG'C
Cuts at: 1132 3365 1132
Size: 2233 3774
SspI AAT'ATT
Cuts at: 5 52 3931 5
Size: 47 3879 2081
Fragments arranged by size:
3879 2081 47
StuI AGG'CCT
Cuts at: 1517 3011 1517
Size: 1494 4513
StyI C'CwwG_G
Cuts at: 513 1065 2004 2085 2349 3303 3446 513
Size: 552 939 81 264 954 143 3074
Fragments arranged by size:
3074 954 939 552 264 143 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2318 2615 2662 3071 4034 4051 4204
Size: 1255 738 297 47 409 963 17 153
Cuts at: 4204 4389 4834 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 963 738 445 409 297 185
153 47 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2216 4253 5965
Size: 274 80 33 51 1443 239 2037 1712
Cuts at: 5965 96
Size: 138
Fragments arranged by size:
2037 1712 1443 274 239 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2637 2669 5967 5999 2637
Size: 32 3298 32 2645
Fragments arranged by size:
3298 2645 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6007
XbaI T'CTAG_A
Cuts at: 3343 3343
Size: 6007
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2065 2335 2065
Size: 270 5737
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6007
XmaI C'CCGG_G
Cuts at: 1870 2028 3332 1870
Size: 158 1304 4545
Fragments arranged by size:
4545 1304 158
XmnI GAAnn'nnTTC
Cuts at: 4136 4136
Size: 6007
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3815 276
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI NaeI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SacII SapI SexAI SfiI
SrfI SwaI