(Linear) (Six-base) MAPSORT of: KIEE5510.seq Check: 333 from: 1 to: 1302 KIEE5510.orf With 182 enzymes: SgfI * December 5, 2014 13:02 .. AccI GT'mk_AC Cuts at: 0 563 1302 Size: 563 739 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 700 1045 1302 Size: 208 492 345 257 Fragments arranged by size: 492 345 257 208 AhdI GACnn_n'nnGTC Cuts at: 0 783 1302 Size: 783 519 AleI CACnn'nnGTG Cuts at: 0 616 1302 Size: 616 686 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1033 1302 Size: 1033 269 AlwNI CAG_nnn'CTG Cuts at: 0 745 1302 Size: 745 557 ApaI G_GGCC'C Cuts at: 0 223 1302 Size: 223 1079 ApoI r'AATT_y Cuts at: 0 274 1302 Size: 274 1028 AvaI C'yCGr_G Cuts at: 0 23 924 1302 Size: 23 901 378 Fragments arranged by size: 901 378 23 BanI G'GyrC_C Cuts at: 0 553 1186 1302 Size: 553 633 116 Fragments arranged by size: 633 553 116 BanII G_rGCy'C Cuts at: 0 223 469 1163 1302 Size: 223 246 694 139 Fragments arranged by size: 694 246 223 139 BbeI G_GCGC'C Cuts at: 0 557 1190 1302 Size: 557 633 112 Fragments arranged by size: 633 557 112 BbsI GAAGACnn'nnnn_ Cuts at: 0 688 884 1302 Size: 688 196 418 Fragments arranged by size: 688 418 196 BclI T'GATC_A Cuts at: 0 455 1302 Size: 455 847 BglI GCCn_nnn'nGGC Cuts at: 0 694 1302 Size: 694 608 BglII A'GATC_T Cuts at: 0 405 1302 Size: 405 897 Bme1580I G_kGCm'C Cuts at: 0 107 223 1302 Size: 107 116 1079 Fragments arranged by size: 1079 116 107 BmrI ACTGGGnnnn_n' Cuts at: 0 101 430 824 909 1302 Size: 101 329 394 85 393 Fragments arranged by size: 394 393 329 101 85 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 24 673 691 1302 Size: 24 649 18 611 Fragments arranged by size: 649 611 24 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 559 1302 Size: 559 743 BsaI GGTCTCn'nnnn_ Cuts at: 0 873 1302 Size: 873 429 BsaHI Gr'CG_yC Cuts at: 0 554 1187 1302 Size: 554 633 115 Fragments arranged by size: 633 554 115 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 451 481 1302 Size: 451 30 821 Fragments arranged by size: 821 451 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1255 1302 Size: 1255 47 BseYI C'CCAG_C Cuts at: 0 412 1091 1302 Size: 412 679 211 Fragments arranged by size: 679 412 211 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 55 1149 1302 Size: 55 1094 153 Fragments arranged by size: 1094 153 55 BsmBI CGTCTCn'nnnn_ Cuts at: 0 143 1302 Size: 143 1159 Bsp1286I G_dGCh'C Cuts at: 0 107 223 469 1163 1302 Size: 107 116 246 694 139 Fragments arranged by size: 694 246 139 116 107 BspHI T'CATG_A Cuts at: 0 503 1229 1302 Size: 503 726 73 Fragments arranged by size: 726 503 73 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 77 1302 Size: 77 1225 BsrBI CCG'CTC Cuts at: 0 1115 1199 1302 Size: 1115 84 103 Fragments arranged by size: 1115 103 84 BsrDI GCAATG_nn' Cuts at: 0 765 1139 1302 Size: 765 374 163 Fragments arranged by size: 765 374 163 BstAPI GCAn_nnn'nTGC Cuts at: 0 1267 1302 Size: 1267 35 BstEII G'GTnAC_C Cuts at: 0 703 1047 1302 Size: 703 344 255 Fragments arranged by size: 703 344 255 BstXI CCAn_nnnn'nTGG Cuts at: 0 618 651 700 1117 1302 Size: 618 33 49 417 185 Fragments arranged by size: 618 417 185 49 33 BstYI r'GATC_y Cuts at: 0 405 1302 Size: 405 897 BtgI C'CryG_G Cuts at: 0 53 119 1302 Size: 53 66 1183 Fragments arranged by size: 1183 66 53 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 384 1302 Size: 384 918 ClaI AT'CG_AT Cuts at: 0 1057 1302 Size: 1057 245 DrdI GACnn_nn'nnGTC Cuts at: 0 961 1302 Size: 961 341 EaeI y'GGCC_r Cuts at: 0 2 77 347 488 686 1211 1302 Size: 2 75 270 141 198 525 91 Fragments arranged by size: 525 270 198 141 91 75 2 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 24 208 673 691 700 1045 1302 Size: 24 184 465 18 9 345 257 Fragments arranged by size: 465 345 257 184 24 18 9 EcoO109I rG'GnC_Cy Cuts at: 0 220 381 666 1302 Size: 220 161 285 636 Fragments arranged by size: 636 285 220 161 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1281 1302 Size: 1281 21 FspI TGC'GCA Cuts at: 0 547 1302 Size: 547 755 FspAI rTGC'GCAy Cuts at: 0 547 1302 Size: 547 755 HaeII r_GCGC'y Cuts at: 0 557 1190 1302 Size: 557 633 112 Fragments arranged by size: 633 557 112 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 292 324 769 801 1302 Size: 292 32 445 32 501 Fragments arranged by size: 501 445 292 32 32 HincII GTy'rAC Cuts at: 0 270 1032 1302 Size: 270 762 270 Fragments arranged by size: 762 270 270 KasI G'GCGC_C Cuts at: 0 553 1186 1302 Size: 553 633 116 Fragments arranged by size: 633 553 116 MscI TGG'CCA Cuts at: 0 4 490 1213 1302 Size: 4 486 723 89 Fragments arranged by size: 723 486 89 4 MslI CAynn'nnrTG Cuts at: 0 616 733 1302 Size: 616 117 569 Fragments arranged by size: 616 569 117 MspA1I CmG'CkG Cuts at: 0 100 370 1302 Size: 100 270 932 Fragments arranged by size: 932 270 100 NarI GG'CG_CC Cuts at: 0 554 1187 1302 Size: 554 633 115 Fragments arranged by size: 633 554 115 PflMI CCAn_nnn'nTGG Cuts at: 0 945 1302 Size: 945 357 PfoI T'CCnGG_A Cuts at: 0 650 1302 Size: 650 652 PpuMI rG'GwC_Cy Cuts at: 0 381 1302 Size: 381 921 PspOMI G'GGCC_C Cuts at: 0 219 1302 Size: 219 1083 PvuII CAG'CTG Cuts at: 0 100 370 1302 Size: 100 270 932 Fragments arranged by size: 932 270 100 SanDI GG'GwC_CC Cuts at: 0 381 1302 Size: 381 921 ScaI AGT'ACT Cuts at: 0 213 1302 Size: 213 1089 SfoI GGC'GCC Cuts at: 0 555 1188 1302 Size: 555 633 114 Fragments arranged by size: 633 555 114 SmaI CCC'GGG Cuts at: 0 25 1302 Size: 25 1277 SmlI C'TyrA_G Cuts at: 0 574 1302 Size: 574 728 StuI AGG'CCT Cuts at: 0 1006 1302 Size: 1006 296 StyI C'CwwG_G Cuts at: 0 80 344 1302 Size: 80 264 958 Fragments arranged by size: 958 264 80 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 313 610 657 1066 1302 Size: 313 297 47 409 236 Fragments arranged by size: 409 313 297 236 47 TatI w'GTAC_w Cuts at: 0 211 1302 Size: 211 1091 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 632 664 1302 Size: 632 32 638 Fragments arranged by size: 638 632 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 60 330 1302 Size: 60 270 972 Fragments arranged by size: 972 270 60 XmaI C'CCGG_G Cuts at: 0 23 1302 Size: 23 1279 Enzymes that do cut: AccI AcuI AhdI AleI AlfI AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI BclI BglI BglII Bme1580I BmrI BpmI BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BstAPI BstEII BstXI BstYI BtgI BtgZI ClaI DrdI EaeI Eco57MI EcoO109I FalI FspI FspAI HaeII Hin4I HincII KasI MscI MslI MspA1I NarI PflMI PfoI PpuMI PspOMI PvuII SanDI ScaI SfoI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BfrBI BlpI BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaWI BsiEI BsiHKAI BsiWI BsmI BspEI BsrFI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtsI CspCI DraI DraIII EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FseI HindIII HpaI KpnI MfeI MluI MmeI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI