################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## Acc65I AclI AcsI AflII AflIII AgeI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AspI AviII AvrII BamHI BbrPI BciVI BfrI BglI BglII BlnI BsaBI BsaI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BspDI BspHI BspLU11I BspMI BsrBI BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KpnI MamI MfeI MluI MseI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AatII 192 GACGTC GACGT^C AccI 277 GTCTAC GT^[AC][GT]AC AccIII 117 TCCGGA T^CCGGA AhdI 187 GACAGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 978 GATCC GGATC(4/5) AocI 8 CCTCAGG CC^T[ACGT]AGG AspEI 187 GACAGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 49 GGCGCC GGCGC^C BbsI 208 GAAGAC GAAGAC(2/6) BbvCI 710 CCTCAGC CCTCAGC(-5/-2) BcgI 435 CGAACTCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 218 TGATCA T^GATCA BlpI 789 GCTGAGC GC^T[ACGT]AGC Bpu1102I 789 GCTGAGC GC^T[ACGT]AGC BpuAI 208 GAAGAC GAAGAC(2/6) BsaAI 280 TACGTG [CT]AC^GT[GA] BsaWI 117 TCCGGA [AT]^CCGG[AT] BseAI 117 TCCGGA T^CCGGA Bsp1407I 140 TGTACA T^GTACA BspEI 117 TCCGGA T^CCGGA BsrDI 358 CATTGC GCAATG(2/0) BsrFI 523 GCCGGC [GA]^CCGG[CT] BsrGI 140 TGTACA T^GTACA BssHII 14 GCGCGC G^CGCGC BstAPI 468 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 645 GGTCACC G^GT[ACGT]ACC BstPI 645 GGTCACC G^GT[ACGT]ACC BstXI 575 CCAGTTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 977 AGATCC [GA]^GATC[CT] Bsu36I 8 CCTCAGG CC^T[ACGT]AGG CelII 789 GCTGAGC GC^T[ACGT]AGC Cfr10I 523 GCCGGC [GA]^CCGG[CT] DsaI 968 CCGCGG C^C[GA][CT]GG Eam1105I 187 GACAGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 8 CCTCAGG CC^T[ACGT]AGG EcoO65I 645 GGTCACC G^GT[ACGT]ACC EcoT14I 1037 CCTTGG C^C[AT][AT]GG FbaI 218 TGATCA T^GATCA HgaI 876 GACGC GACGC(5/10) HincII 370 GTCAAC GT[CT]^[GA]AC HindII 370 GTCAAC GT[CT]^[GA]AC KasI 49 GGCGCC G^GCGCC KspI 968 CCGCGG CCGC^GG MflI 977 AGATCC [GA]^GATC[CT] MroI 117 TCCGGA T^CCGGA NaeI 523 GCCGGC GCC^GGC NarI 49 GGCGCC GG^CGCC NgoMIV 523 GCCGGC G^CCGGC NspI 1022 GCATGT [GA]CATG^[CT] PflMI 849 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacII 968 CCGCGG CCGC^GG ScaI 62 AGTACT AGT^ACT SfoI 49 GGCGCC GGC^GCC SspBI 140 TGTACA T^GTACA StyI 1037 CCTTGG C^C[AT][AT]GG Van91I 849 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 56 CCAACAAGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 977 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### ApaI 551 GGGCCC GGGCC^C 1056 GGGCCC BfaI 448 CTAG C^TAG 719 CTAG BsmFI 103 GGGAC GGGAC(10/14) 93 GTCCC DpnII 219 GATC ^GATC 978 GATC EarI 666 CTCTTC CTCTTC(1/4) 1198 GAAGAG HaeII 49 GGCGCC [GA]GCGC^[CT] 923 AGCGCC HphI 454 GGTGA GGTGA(8/7) 647 TCACC Ksp632I 666 CTCTTC CTCTTC(1/4) 1198 GAAGAG MaeI 448 CTAG C^TAG 719 CTAG MaeIII 112 GTGAC ^GT[ACGT]AC 646 GTCAC MboI 219 GATC ^GATC 978 GATC NdeII 219 GATC ^GATC 978 GATC PstI 466 CTGCAG CTGCA^G 1085 CTGCAG Sau3AI 219 GATC ^GATC 978 GATC SfcI 466 CTGCAG C^T[GA][CT]AG 1085 CTGCAG Tsp45I 112 GTGAC ^GT[GC]AC 646 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwNI AvaI AvaII BalI BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BseRI BsiYI BslI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII ItaI MaeII MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI PvuII RsaI Sau96I ScrFI SfaNI TseI TspRI