Restriction map for
pFN21AE1491
(Circular) (Six-base) MAPSORT of: pFN21AE1491.seq Check: 6114 from: 1 to: 5926
pFN21AE1491 5926 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2201 3736 278
Size: 53 83 186 936 665 1535 2468
Fragments arranged by size:
2468 1535 936 665 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2283 3269 1937
Size: 346 986 4594
Fragments arranged by size:
4594 986 346
Acc65I G'GTAC_C
Cuts at: 3247 3247
Size: 5926
AclI AA'CG_TT
Cuts at: 1563 4053 4426 1563
Size: 2490 373 3063
Fragments arranged by size:
3063 2490 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2123 2312 3989 5373 5641 1632
Size: 491 189 1677 1384 268 1917
Fragments arranged by size:
1917 1677 1384 491 268 189
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5729
AflIII A'CryG_T
Cuts at: 1203 4825 5802 1203
Size: 3622 977 1327
Fragments arranged by size:
3622 1327 977
AgeI A'CCGG_T
Cuts at: 4757 4757
Size: 5926
AhdI GACnn_n'nnGTC
Cuts at: 2197 4655 2197
Size: 2458 3468
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5926
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5926
AlwNI CAG_nnn'CTG
Cuts at: 2479 2711 3022 3098 3669 5241 2479
Size: 232 311 76 571 1572 3164
Fragments arranged by size:
3164 1572 571 311 232 76
ApaI G_GGCC'C
Cuts at: 2560 3065 2560
Size: 505 5421
ApaLI G'TGCA_C
Cuts at: 3983 5139 3983
Size: 1156 4770
ApoI r'AATT_y
Cuts at: 1493 3235 3461 3613 5722 1493
Size: 1742 226 152 2109 1697
Fragments arranged by size:
2109 1742 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4480 160
Size: 872 3448 1606
Fragments arranged by size:
3448 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
AvaI C'yCGr_G
Cuts at: 1870 1943 2049 2728 3049 3164 3251 1870
Size: 73 106 679 321 115 87 4545
Fragments arranged by size:
4545 679 321 115 106 87 73
BamHI G'GATC_C
Cuts at: 3256 3623 3256
Size: 367 5559
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2038 2054 2257 3247 4702
Size: 325 217 627 251 16 203 990 1455
Cuts at: 4702 618
Size: 1842
Fragments arranged by size:
1842 1455 990 627 325 251 217 203
16
BanII G_rGCy'C
Cuts at: 1391 2560 2735 3065 3245 1391
Size: 1169 175 330 180 4072
Fragments arranged by size:
4072 1169 330 180 175
BbeI G_GCGC'C
Cuts at: 2058 2058
Size: 5926
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2220 928
Size: 971 321 4634
Fragments arranged by size:
4634 971 321
BbvCI CC'TCA_GC
Cuts at: 2716 2716
Size: 5926
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2429 2463 4117 4151 1903
Size: 34 492 34 1654 34 3678
Fragments arranged by size:
3678 1654 492 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3818 5028 3818
Size: 1210 4716
BclI T'GATC_A
Cuts at: 1548 2223 1548
Size: 675 5251
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4537 136
Size: 107 122 71 631 605 2865 1525
Fragments arranged by size:
2865 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5921 5921
Size: 5926
BlpI GC'TnA_GC
Cuts at: 2795 3343 2795
Size: 548 5378
Bme1580I G_kGCm'C
Cuts at: 1790 2270 2413 2560 3065 3987 5143 1790
Size: 480 143 147 505 922 1156 2573
Fragments arranged by size:
2573 1156 922 505 480 147 143
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5926
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2423 2552 2597
Size: 416 53 83 399 24 1006 129 45
Cuts at: 2597 2736 3001 3657 4615 442
Size: 139 265 656 958 1753
Fragments arranged by size:
1753 1006 958 656 416 399 265 139
129 83 53 45 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2589 2608 2688 2789 4586 1239
Size: 1350 19 80 101 1797 2579
Fragments arranged by size:
2579 1797 1350 101 80 19
Bpu10I CC'TnA_GC
Cuts at: 2716 2716
Size: 5926
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4051 4916 5214 4051
Size: 865 298 4763
Fragments arranged by size:
4763 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4589 882
Size: 617 3090 2219
Fragments arranged by size:
3090 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2287 3717 493
Size: 711 1083 1430 2702
Fragments arranged by size:
2702 1430 1083 711
BsaBI GATnn'nnATC
Cuts at: 5920 5920
Size: 5926
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2055 2198 2882 3733
Size: 53 83 186 936 522 143 684 851
Cuts at: 3733 4115 4750 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 851 684 635 522 382 186
143 83 53
BsaWI w'CCGG_w
Cuts at: 2122 4359 4757 5031 5178 5589 2122
Size: 2237 398 274 147 411 2459
Fragments arranged by size:
2459 2237 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2104 2134 1178
Size: 30 165 30 701 30 4970
Fragments arranged by size:
4970 701 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2372 2608 2611 3221 1186
Size: 1186 236 3 610 3891
Fragments arranged by size:
3891 1186 610 236 3
BseYI C'CCAG_C
Cuts at: 2270 2482 2551 2689 2781 3058 5129 5625 2270
Size: 212 69 138 92 277 2071 496 2571
Fragments arranged by size:
2571 2071 496 277 212 138 92 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5353
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3376 4137 5165 1540
Size: 275 186 1375 761 1028 2301
Fragments arranged by size:
2301 1375 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3987 4072 5143 3987
Size: 85 1071 4770
Fragments arranged by size:
4770 1071 85
BsmI GAATG_Cn'
Cuts at: 3437 3530 3437
Size: 93 5833
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2270 2413 2560 2735 3065 3245 3987
Size: 399 480 143 147 175 330 180 742
Cuts at: 3987 4072 5143 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 480 399 330 180 175
147 143 85
BspEI T'CCGG_A
Cuts at: 2122 5589 2122
Size: 3467 2459
BspHI T'CATG_A
Cuts at: 3815 5545 3815
Size: 1730 4196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3281 844
Size: 2437 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3813 1394
Size: 429 1990 3507
Fragments arranged by size:
3507 1990 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2362 4421 4595 65
Size: 1183 1114 2059 174 1396
Fragments arranged by size:
2059 1396 1183 1114 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2528 4570 4757 1700
Size: 110 718 2042 187 2869
Fragments arranged by size:
2869 2042 718 187 110
BsrGI T'GTAC_A
Cuts at: 96 2145 96
Size: 2049 3877
BssHII G'CGCG_C
Cuts at: 1925 2019 1925
Size: 94 5832
BssSI C'ACGA_G
Cuts at: 3986 4998 3986
Size: 1012 4914
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2479 794
Size: 1685 4241
BstBI TT'CG_AA
Cuts at: 4730 4730
Size: 5926
BstEII G'GTnAC_C
Cuts at: 1184 2650 1184
Size: 1466 4460
BstXI CCAn_nnnn'nTGG
Cuts at: 2587 2587
Size: 5926
BstYI r'GATC_y
Cuts at: 1972 2982 3256 3623 4008 4025 5477 5563 5575
Size: 1010 274 367 385 17 1452 86 12
Cuts at: 5575 5921 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1010 385 367 346 274 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2014 2014
Size: 5926
BtgI C'CryG_G
Cuts at: 513 1065 2973 513
Size: 552 1908 3466
Fragments arranged by size:
3466 1908 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3135 487
Size: 683 56 1909 3278
Fragments arranged by size:
3278 1909 683 56
BtsI GCAGTG_nn'
Cuts at: 2270 2602 3442 4236 4256 2270
Size: 332 840 794 20 3940
Fragments arranged by size:
3940 840 794 332 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5926
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5891
DraI TTT'AAA
Cuts at: 3230 3230
Size: 5926
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4933 809
Size: 507 3617 1802
Fragments arranged by size:
3617 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2434 2577 2817
Size: 54 1425 50 275 18 604 143 240
Cuts at: 2817 2851 2868 3373 4262 5891 8
Size: 34 17 505 889 1629 43
Fragments arranged by size:
1629 1425 889 604 505 275 240 143
54 50 43 34 18 17
EagI C'GGCC_G
Cuts at: 1537 1812 3373 1537
Size: 275 1561 4090
Fragments arranged by size:
4090 1561 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2677 3198 3856 1353
Size: 1324 521 658 3423
Fragments arranged by size:
3423 1324 658 521
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4513 4887 5033 387
Size: 1064 138 2924 374 146 1280
Fragments arranged by size:
2924 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2312 2589 2608 2688 2789 3989
Size: 393 491 189 277 19 80 101 1200
Cuts at: 3989 4586 5373 5641 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1200 787 597 491 393 277 268
189 101 80 19
EcoO109I rG'GnC_Cy
Cuts at: 2556 2557 3019 3062 2556
Size: 1 462 43 5420
Fragments arranged by size:
5420 462 43 1
EcoRI G'AATT_C
Cuts at: 3235 3613 3235
Size: 378 5548
FspI TGC'GCA
Cuts at: 4432 4432
Size: 5926
HaeII r_GCGC'y
Cuts at: 1678 2058 2932 5073 1678
Size: 380 874 2141 2531
Fragments arranged by size:
2531 2141 874 380
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2183 2215 2881 2913 4567
Size: 32 135 32 611 32 666 32 1654
Cuts at: 4567 4599 4641 4673 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1654 666 611 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2377 3270 3516 669
Size: 988 281 439 893 246 3079
Fragments arranged by size:
3079 988 893 439 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5926
HpaI GTT'AAC
Cuts at: 3516 3516
Size: 5926
KasI G'GCGC_C
Cuts at: 2054 2054
Size: 5926
KpnI G_GTAC'C
Cuts at: 3251 3251
Size: 5926
MfeI C'AATT_G
Cuts at: 3525 3525
Size: 5926
MluI A'CGCG_T
Cuts at: 5802 5802
Size: 5926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2085 5040 5224 1305
Size: 780 2955 184 2007
Fragments arranged by size:
2955 2007 780 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2579 2819 2870 10
Size: 54 1425 343 747 240 51 3066
Fragments arranged by size:
3066 1425 747 343 240 54 51
MslI CAynn'nnrTG
Cuts at: 518 1166 3884 4243 4402 518
Size: 648 2718 359 159 2042
Fragments arranged by size:
2718 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2274 2424 2433 2648 2711 2794 2975 3058
Size: 668 150 9 215 63 83 181 83
Cuts at: 3058 3342 4019 5167 5412 1606
Size: 284 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 668 284 245 215 181
150 83 83 63 9
NaeI GCC'GGC
Cuts at: 2530 2530
Size: 5926
NarI GG'CG_CC
Cuts at: 2055 2055
Size: 5926
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5374
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5926
NgoMIV G'CCGG_C
Cuts at: 2528 2528
Size: 5926
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5926
NotI GC'GGCC_GC
Cuts at: 3373 3373
Size: 5926
NspI r_CATG'y
Cuts at: 1132 1229 3031 3284 4829 1132
Size: 97 1802 253 1545 2229
Fragments arranged by size:
2229 1802 1545 253 97
PasI CC'CwG_GG
Cuts at: 1348 3022 1348
Size: 1674 4252
PciI A'CATG_T
Cuts at: 4825 4825
Size: 5926
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2860 1108
Size: 1752 4174
PfoI T'CCnGG_A
Cuts at: 2201 2630 2747 2922 2201
Size: 429 117 175 5205
Fragments arranged by size:
5205 429 175 117
PmeI GTTT'AAAC
Cuts at: 3230 3230
Size: 5926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3995 4027 5508 5540 3995
Size: 32 1481 32 4381
Fragments arranged by size:
4381 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5926
PsiI TTA'TAA
Cuts at: 3496 3496
Size: 5926
PspOMI G'GGCC_C
Cuts at: 2556 3061 2556
Size: 505 5421
PstI C_TGCA'G
Cuts at: 830 2475 3094 3278 830
Size: 1645 619 184 3478
Fragments arranged by size:
3478 1645 619 184
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5926
PvuII CAG'CTG
Cuts at: 2711 2794 3058 2711
Size: 83 264 5579
Fragments arranged by size:
5579 264 83
SacII CC_GC'GG
Cuts at: 2976 2976
Size: 5926
SalI G'TCGA_C
Cuts at: 1936 3268 1936
Size: 1332 4594
SbfI CC_TGCA'GG
Cuts at: 3278 3278
Size: 5926
ScaI AGT'ACT
Cuts at: 2069 4174 5886 2069
Size: 2105 1712 2109
Fragments arranged by size:
2109 2105 1712
SfcI C'TryA_G
Cuts at: 826 1045 2471 3090 3274 4409 5090 5281 826
Size: 219 1426 619 184 1135 681 191 1471
Fragments arranged by size:
1471 1426 1135 681 619 219 191 184
SfoI GGC'GCC
Cuts at: 2056 2056
Size: 5926
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5926
SmaI CCC'GGG
Cuts at: 1872 3253 1872
Size: 1381 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4030 4931 5193 820
Size: 197 926 2087 901 262 1553
Fragments arranged by size:
2087 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3717 493
Size: 3224 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5926
SphI G_CATG'C
Cuts at: 1132 3284 1132
Size: 2152 3774
SspI AAT'ATT
Cuts at: 5 52 3850 5
Size: 47 3798 2081
Fragments arranged by size:
3798 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5926
StyI C'CwwG_G
Cuts at: 513 1065 3042 3365 513
Size: 552 1977 323 3074
Fragments arranged by size:
3074 1977 552 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2534 3071 3953 3970 4123 4308 4753
Size: 1255 954 537 882 17 153 185 445
Cuts at: 4753 325
Size: 1498
Fragments arranged by size:
1498 1255 954 882 537 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2067 2145 4172
Size: 274 80 33 51 1443 90 78 2027
Cuts at: 4172 5884 96
Size: 1712 138
Fragments arranged by size:
2027 1712 1443 274 138 90 80 78
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2104 2136 5886 5918 2104
Size: 32 3750 32 2112
Fragments arranged by size:
3750 2112 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5926
XbaI T'CTAG_A
Cuts at: 3262 3262
Size: 5926
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2068 2068
Size: 5926
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5926
XmaI C'CCGG_G
Cuts at: 1870 3251 1870
Size: 1381 4545
XmnI GAAnn'nnTTC
Cuts at: 4055 4055
Size: 5926
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2199 3734 276
Size: 53 83 186 936 665 1535 2468
Fragments arranged by size:
2468 1535 936 665 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI
SwaI