(Linear) (Six-base) MAPSORT of: KIEE1491.seq Check: 6592 from: 1 to: 1221 KIEE1491.orf With 182 enzymes: SgfI * July 15, 2010 18:40 .. AatII G_ACGT'C Cuts at: 0 196 1221 Size: 196 1025 AccI GT'mk_AC Cuts at: 0 278 1221 Size: 278 943 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 118 307 1221 Size: 118 189 914 Fragments arranged by size: 914 189 118 AhdI GACnn_n'nnGTC Cuts at: 0 192 1221 Size: 192 1029 AlwNI CAG_nnn'CTG Cuts at: 0 474 706 1017 1093 1221 Size: 474 232 311 76 128 Fragments arranged by size: 474 311 232 128 76 ApaI G_GGCC'C Cuts at: 0 555 1060 1221 Size: 555 505 161 Fragments arranged by size: 555 505 161 AvaI C'yCGr_G Cuts at: 0 44 723 1044 1159 1221 Size: 44 679 321 115 62 Fragments arranged by size: 679 321 115 62 44 BanI G'GyrC_C Cuts at: 0 33 49 252 1221 Size: 33 16 203 969 Fragments arranged by size: 969 203 33 16 BanII G_rGCy'C Cuts at: 0 555 730 1060 1221 Size: 555 175 330 161 Fragments arranged by size: 555 330 175 161 BbeI G_GCGC'C Cuts at: 0 53 1221 Size: 53 1168 BbsI GAAGACnn'nnnn_ Cuts at: 0 215 1221 Size: 215 1006 BbvCI CC'TCA_GC Cuts at: 0 711 1221 Size: 711 510 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 424 458 1221 Size: 424 34 763 Fragments arranged by size: 763 424 34 BclI T'GATC_A Cuts at: 0 218 1221 Size: 218 1003 BlpI GC'TnA_GC Cuts at: 0 790 1221 Size: 790 431 Bme1580I G_kGCm'C Cuts at: 0 265 408 555 1060 1221 Size: 265 143 147 505 161 Fragments arranged by size: 505 265 161 147 143 BmrI ACTGGGnnnn_n' Cuts at: 0 418 547 592 731 996 1221 Size: 418 129 45 139 265 225 Fragments arranged by size: 418 265 225 139 129 45 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 584 603 683 784 1221 Size: 584 19 80 101 437 Fragments arranged by size: 584 437 101 80 19 Bpu10I CC'TnA_GC Cuts at: 0 711 1221 Size: 711 510 BsaAI yAC'GTr Cuts at: 0 282 1221 Size: 282 939 BsaHI Gr'CG_yC Cuts at: 0 50 193 877 1221 Size: 50 143 684 344 Fragments arranged by size: 684 344 143 50 BsaWI w'CCGG_w Cuts at: 0 117 1221 Size: 117 1104 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 99 129 1221 Size: 99 30 1092 Fragments arranged by size: 1092 99 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 367 603 606 1216 1221 Size: 367 236 3 610 5 Fragments arranged by size: 610 367 236 5 3 BseYI C'CCAG_C Cuts at: 0 265 477 546 684 776 1053 1221 Size: 265 212 69 138 92 277 168 Fragments arranged by size: 277 265 212 168 138 92 69 Bsp1286I G_dGCh'C Cuts at: 0 265 408 555 730 1060 1221 Size: 265 143 147 175 330 161 Fragments arranged by size: 330 265 175 161 147 143 BspEI T'CCGG_A Cuts at: 0 117 1221 Size: 117 1104 BsrDI GCAATG_nn' Cuts at: 0 357 1221 Size: 357 864 BsrFI r'CCGG_y Cuts at: 0 523 1221 Size: 523 698 BsrGI T'GTAC_A Cuts at: 0 140 1221 Size: 140 1081 BssHII G'CGCG_C Cuts at: 0 14 1221 Size: 14 1207 BstAPI GCAn_nnn'nTGC Cuts at: 0 474 1221 Size: 474 747 BstEII G'GTnAC_C Cuts at: 0 645 1221 Size: 645 576 BstXI CCAn_nnnn'nTGG Cuts at: 0 582 1221 Size: 582 639 BstYI r'GATC_y Cuts at: 0 977 1221 Size: 977 244 Bsu36I CC'TnA_GG Cuts at: 0 9 1221 Size: 9 1212 BtgI C'CryG_G Cuts at: 0 968 1221 Size: 968 253 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1130 1221 Size: 1130 91 BtsI GCAGTG_nn' Cuts at: 0 265 597 1221 Size: 265 332 624 Fragments arranged by size: 624 332 265 EaeI y'GGCC_r Cuts at: 0 429 572 812 846 863 1221 Size: 429 143 240 34 17 358 Fragments arranged by size: 429 358 240 143 34 17 EarI CTCTTCn'nnn_ Cuts at: 0 672 1193 1221 Size: 672 521 28 Fragments arranged by size: 672 521 28 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 118 307 584 603 683 784 1221 Size: 118 189 277 19 80 101 437 Fragments arranged by size: 437 277 189 118 101 80 19 EcoO109I rG'GnC_Cy Cuts at: 0 551 552 1014 1057 1221 Size: 551 1 462 43 164 Fragments arranged by size: 551 462 164 43 1 HaeII r_GCGC'y Cuts at: 0 53 927 1221 Size: 53 874 294 Fragments arranged by size: 874 294 53 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 178 210 876 908 1221 Size: 178 32 666 32 313 Fragments arranged by size: 666 313 178 32 32 HincII GTy'rAC Cuts at: 0 372 1221 Size: 372 849 KasI G'GCGC_C Cuts at: 0 49 1221 Size: 49 1172 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 80 1221 Size: 80 1141 MscI TGG'CCA Cuts at: 0 574 814 865 1221 Size: 574 240 51 356 Fragments arranged by size: 574 356 240 51 MspA1I CmG'CkG Cuts at: 0 269 419 428 643 706 789 970 1053 Size: 269 150 9 215 63 83 181 83 Cuts at: 1053 1221 Size: 168 Fragments arranged by size: 269 215 181 168 150 83 83 63 9 NaeI GCC'GGC Cuts at: 0 525 1221 Size: 525 696 NarI GG'CG_CC Cuts at: 0 50 1221 Size: 50 1171 NgoMIV G'CCGG_C Cuts at: 0 523 1221 Size: 523 698 NspI r_CATG'y Cuts at: 0 1026 1221 Size: 1026 195 PasI CC'CwG_GG Cuts at: 0 1017 1221 Size: 1017 204 PflMI CCAn_nnn'nTGG Cuts at: 0 855 1221 Size: 855 366 PfoI T'CCnGG_A Cuts at: 0 196 625 742 917 1221 Size: 196 429 117 175 304 Fragments arranged by size: 429 304 196 175 117 PspOMI G'GGCC_C Cuts at: 0 551 1056 1221 Size: 551 505 165 Fragments arranged by size: 551 505 165 PstI C_TGCA'G Cuts at: 0 470 1089 1221 Size: 470 619 132 Fragments arranged by size: 619 470 132 PvuII CAG'CTG Cuts at: 0 706 789 1053 1221 Size: 706 83 264 168 Fragments arranged by size: 706 264 168 83 SacII CC_GC'GG Cuts at: 0 971 1221 Size: 971 250 ScaI AGT'ACT Cuts at: 0 64 1221 Size: 64 1157 SfcI C'TryA_G Cuts at: 0 466 1085 1221 Size: 466 619 136 Fragments arranged by size: 619 466 136 SfoI GGC'GCC Cuts at: 0 51 1221 Size: 51 1170 StyI C'CwwG_G Cuts at: 0 1037 1221 Size: 1037 184 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 529 1066 1221 Size: 529 537 155 Fragments arranged by size: 537 529 155 TatI w'GTAC_w Cuts at: 0 62 140 1221 Size: 62 78 1081 Fragments arranged by size: 1081 78 62 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 99 131 1221 Size: 99 32 1090 Fragments arranged by size: 1090 99 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 63 1221 Size: 63 1158 ZraI GAC'GTC Cuts at: 0 194 1221 Size: 194 1027 Enzymes that do cut: AatII AccI AcuI AhdI AlwNI ApaI AvaI BanI BanII BbeI BbsI BbvCI BcgI BclI BlpI Bme1580I BmrI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI Bsp1286I BspEI BsrDI BsrFI BsrGI BssHII BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI Eco57MI EcoO109I HaeII Hin4I HincII KasI MmeI MscI MspA1I NaeI NarI NgoMIV NspI PasI PflMI PfoI PspOMI PstI PvuII SacII ScaI SfcI SfoI StyI TaqII TatI TstI XcmI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI BfrBI BglI BglII BmgBI BmtI BplI BpuEI BsaI BsaBI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI BspMI BsrBI BssSI BstBI BstZ17I ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MslI NcoI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI