(Circular) (Six-base) MAPSORT of: pFN21AB6195.seq Check: 9291 from: 1 to: 6694 pFN21AB6195 6694 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4504 278 Size: 53 83 186 936 2968 2468 Fragments arranged by size: 2968 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4037 1937 Size: 2100 4594 Acc65I G'GTAC_C Cuts at: 2122 4015 2122 Size: 1893 4801 AclI AA'CG_TT Cuts at: 1563 4821 5194 1563 Size: 3258 373 3063 Fragments arranged by size: 3258 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2329 3183 3711 3716 4757 6141 6409 1632 Size: 697 854 528 5 1041 1384 268 1917 Fragments arranged by size: 1917 1384 1041 854 697 528 268 5 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6497 AflIII A'CryG_T Cuts at: 1203 5593 6570 1203 Size: 4390 977 1327 Fragments arranged by size: 4390 1327 977 AgeI A'CCGG_T Cuts at: 5525 5525 Size: 6694 AhdI GACnn_n'nnGTC Cuts at: 5423 5423 Size: 6694 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6694 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2258 1184 Size: 1074 5620 AlwNI CAG_nnn'CTG Cuts at: 2227 2860 3524 3842 4437 6009 2227 Size: 633 664 318 595 1572 2912 Fragments arranged by size: 2912 1572 664 633 595 318 ApaI G_GGCC'C Cuts at: 3540 3667 3540 Size: 127 6567 ApaLI G'TGCA_C Cuts at: 4751 5907 4751 Size: 1156 5538 ApoI r'AATT_y Cuts at: 1493 3060 4003 4229 4381 6490 1493 Size: 1567 943 226 152 2109 1697 Fragments arranged by size: 2109 1697 1567 943 226 152 AseI AT'TA_AT Cuts at: 160 1032 5248 160 Size: 872 4216 1606 Fragments arranged by size: 4216 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6694 AvaI C'yCGr_G Cuts at: 1870 1943 2152 4019 1870 Size: 73 209 1867 4545 Fragments arranged by size: 4545 1867 209 73 AvrII C'CTAG_G Cuts at: 3220 3605 3220 Size: 385 6309 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2545 2578 2545 Size: 33 6661 BamHI G'GATC_C Cuts at: 4024 4391 4024 Size: 367 6327 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2122 3502 4015 5470 618 Size: 325 217 627 335 1380 513 1455 1842 Fragments arranged by size: 1842 1455 1380 627 513 335 325 217 BanII G_rGCy'C Cuts at: 1391 2427 3248 3442 3540 3667 3711 3855 3984 Size: 1036 821 194 98 127 44 144 129 Cuts at: 3984 4013 1391 Size: 29 4072 Fragments arranged by size: 4072 1036 821 194 144 129 127 98 44 29 BbeI G_GCGC'C Cuts at: 3506 3506 Size: 6694 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2091 2519 2549 928 Size: 971 192 428 30 5073 Fragments arranged by size: 5073 971 428 192 30 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4885 4919 1903 Size: 34 2948 34 3678 Fragments arranged by size: 3678 2948 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4586 5796 4586 Size: 1210 5484 BclI T'GATC_A Cuts at: 1548 1548 Size: 6694 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2957 2966 3437 Size: 107 122 71 631 605 1285 9 471 Cuts at: 3437 3786 5305 136 Size: 349 1519 1525 Fragments arranged by size: 1525 1519 1285 631 605 471 349 122 107 71 9 BglII A'GATC_T Cuts at: 2340 3014 6689 2340 Size: 674 3675 2345 Fragments arranged by size: 3675 2345 674 BlpI GC'TnA_GC Cuts at: 3885 4111 3885 Size: 226 6468 Bme1580I G_kGCm'C Cuts at: 1790 2809 3207 3540 3667 4755 5911 1790 Size: 1019 398 333 127 1088 1156 2573 Fragments arranged by size: 2573 1156 1088 1019 398 333 127 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6694 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2019 2526 3830 Size: 416 53 83 399 24 602 507 1304 Cuts at: 3830 4425 5383 442 Size: 595 958 1753 Fragments arranged by size: 1753 1304 958 602 595 507 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6694 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2284 2506 3874 2284 Size: 222 1368 5104 Fragments arranged by size: 5104 1368 222 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2180 2476 2498 2983 3062 3138 3320 5354 Size: 941 296 22 485 79 76 182 2034 Cuts at: 5354 1239 Size: 2579 Fragments arranged by size: 2579 2034 941 485 296 182 79 76 22 Bpu10I CC'TnA_GC Cuts at: 3865 3865 Size: 6694 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3869 4819 5684 5982 3869 Size: 950 865 298 4581 Fragments arranged by size: 4581 950 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3010 3080 3689 5357 882 Size: 617 1511 70 609 1668 2219 Fragments arranged by size: 2219 1668 1511 617 609 70 BsaAI yAC'GTr Cuts at: 493 1204 4485 493 Size: 711 3281 2702 Fragments arranged by size: 3281 2702 711 BsaBI GATnn'nnATC Cuts at: 6688 6688 Size: 6694 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3503 4501 4883 5518 Size: 53 83 186 936 1970 998 382 635 Cuts at: 5518 275 Size: 1451 Fragments arranged by size: 1970 1451 998 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2538 5127 5525 5799 5946 6357 2538 Size: 2589 398 274 147 411 2875 Fragments arranged by size: 2875 2589 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3301 3331 3787 3817 1178 Size: 30 165 30 1898 30 456 30 4055 Fragments arranged by size: 4055 1898 456 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2495 3134 3625 3795 3847 1186 Size: 1309 639 491 170 52 4033 Fragments arranged by size: 4033 1309 639 491 170 52 BseYI C'CCAG_C Cuts at: 2101 2315 2582 2700 3206 3476 3539 3809 3934 Size: 214 267 118 506 270 63 270 125 Cuts at: 3934 5897 6393 2101 Size: 1963 496 2402 Fragments arranged by size: 2402 1963 506 496 270 270 267 214 125 118 63 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2364 1163 Size: 573 628 5493 Fragments arranged by size: 5493 628 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4144 4905 5933 1540 Size: 275 186 2143 761 1028 2301 Fragments arranged by size: 2301 2143 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3711 3855 4755 4840 5911 3711 Size: 144 900 85 1071 4494 Fragments arranged by size: 4494 1071 900 144 85 BsmI GAATG_Cn' Cuts at: 2470 4205 4298 2470 Size: 1735 93 4866 Fragments arranged by size: 4866 1735 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3154 3154 Size: 6694 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2427 2809 3207 3248 3442 3540 3667 Size: 399 637 382 398 41 194 98 127 Cuts at: 3667 3711 3855 3984 4013 4755 4840 5911 1391 Size: 44 144 129 29 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 637 399 398 382 194 144 129 127 98 85 44 41 29 BspEI T'CCGG_A Cuts at: 2538 6357 2538 Size: 3819 2875 BspHI T'CATG_A Cuts at: 4583 6313 4583 Size: 1730 4964 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2110 4049 844 Size: 1266 1939 3489 Fragments arranged by size: 3489 1939 1266 BsrBI CCG'CTC Cuts at: 1394 1823 4581 1394 Size: 429 2758 3507 Fragments arranged by size: 3507 2758 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3564 5189 5363 65 Size: 1183 2316 1625 174 1396 Fragments arranged by size: 2316 1625 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5338 5525 1700 Size: 110 3528 187 2869 Fragments arranged by size: 3528 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6694 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6694 BssSI C'ACGA_G Cuts at: 4754 5766 4754 Size: 1012 5682 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6694 BstBI TT'CG_AA Cuts at: 5498 5498 Size: 6694 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6694 BstXI CCAn_nnnn'nTGG Cuts at: 2893 2893 Size: 6694 BstYI r'GATC_y Cuts at: 1972 2223 2340 3014 3123 3923 4024 4391 4776 Size: 251 117 674 109 800 101 367 385 Cuts at: 4776 4793 6245 6331 6343 6689 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 800 674 385 367 346 251 117 109 101 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2946 3701 3859 2946 Size: 755 158 5781 Fragments arranged by size: 5781 755 158 BtgI C'CryG_G Cuts at: 513 1065 2004 2286 2955 3234 3279 3510 3669 Size: 552 939 282 669 279 45 231 159 Cuts at: 3669 513 Size: 3538 Fragments arranged by size: 3538 939 669 552 282 279 231 159 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5955 Fragments arranged by size: 5955 683 56 BtsI GCAGTG_nn' Cuts at: 4210 5004 5024 4210 Size: 794 20 5880 Fragments arranged by size: 5880 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6694 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3732 3767 529 Size: 35 3168 35 3456 Fragments arranged by size: 3456 3168 35 35 DraI TTT'AAA Cuts at: 3998 3998 Size: 6694 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2897 5701 809 Size: 507 1581 2804 1802 Fragments arranged by size: 2804 1802 1581 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2838 4141 5030 Size: 54 1425 50 275 18 1008 1303 889 Cuts at: 5030 6659 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1303 1008 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4141 1537 Size: 275 2329 4090 Fragments arranged by size: 4090 2329 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2525 2661 3046 3687 4624 1353 Size: 1172 136 385 641 937 3423 Fragments arranged by size: 3423 1172 937 641 385 136 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5281 5655 5801 387 Size: 1064 138 3692 374 146 1280 Fragments arranged by size: 3692 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3709 3853 3709 Size: 144 6550 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2180 2329 2476 2498 2983 3062 3138 Size: 393 548 149 147 22 485 79 76 Cuts at: 3138 3183 3320 3711 3716 4757 5354 6141 6409 Size: 45 137 391 5 1041 597 787 268 Cuts at: 6409 1239 Size: 1524 Fragments arranged by size: 1524 1041 787 597 548 485 393 391 268 149 147 137 79 76 45 22 5 EcoNI CCTnn'n_nnAGG Cuts at: 3577 3577 Size: 6694 EcoO109I rG'GnC_Cy Cuts at: 2199 2890 3536 3630 3663 3664 3870 2199 Size: 691 646 94 33 1 206 5023 Fragments arranged by size: 5023 691 646 206 94 33 1 EcoRI G'AATT_C Cuts at: 4003 4381 4003 Size: 378 6316 EcoRV GAT'ATC Cuts at: 2071 2323 2071 Size: 252 6442 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2359 3388 2359 Size: 1029 5665 FspI TGC'GCA Cuts at: 5200 5200 Size: 6694 HaeII r_GCGC'y Cuts at: 1678 3506 5841 1678 Size: 1828 2335 2531 Fragments arranged by size: 2531 2335 1828 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2203 2235 3787 3819 5335 Size: 32 135 32 631 32 1552 32 1516 Cuts at: 5335 5367 5409 5441 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1552 1516 631 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2283 4038 4284 669 Size: 988 281 345 1755 246 3079 Fragments arranged by size: 3079 1755 988 345 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6694 HpaI GTT'AAC Cuts at: 4284 4284 Size: 6694 KasI G'GCGC_C Cuts at: 3502 3502 Size: 6694 KpnI G_GTAC'C Cuts at: 2126 4019 2126 Size: 1893 4801 MfeI C'AATT_G Cuts at: 4293 4293 Size: 6694 MluI A'CGCG_T Cuts at: 6570 6570 Size: 6694 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2145 3515 3767 5808 5992 1305 Size: 840 1370 252 2041 184 2007 Fragments arranged by size: 2041 2007 1370 840 252 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2840 10 Size: 54 1425 343 1008 3864 Fragments arranged by size: 3864 1425 1008 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3974 4652 5011 5170 518 Size: 648 2808 678 359 159 2042 Fragments arranged by size: 2808 2042 678 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2080 2105 2288 2387 3350 4110 4787 5935 Size: 474 25 183 99 963 760 677 1148 Cuts at: 5935 6180 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 963 760 677 474 245 183 99 25 NarI GG'CG_CC Cuts at: 3503 3503 Size: 6694 NcoI C'CATG_G Cuts at: 513 1065 2004 3669 513 Size: 552 939 1665 3538 Fragments arranged by size: 3538 1665 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6694 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6694 NotI GC'GGCC_GC Cuts at: 4141 4141 Size: 6694 NspI r_CATG'y Cuts at: 1132 1229 3071 4052 5597 1132 Size: 97 1842 981 1545 2229 Fragments arranged by size: 2229 1842 1545 981 97 PasI CC'CwG_GG Cuts at: 1348 3025 3434 3554 1348 Size: 1677 409 120 4488 Fragments arranged by size: 4488 1677 409 120 PciI A'CATG_T Cuts at: 5593 5593 Size: 6694 PflMI CCAn_nnn'nTGG Cuts at: 1108 2080 2754 3472 1108 Size: 972 674 718 4330 Fragments arranged by size: 4330 972 718 674 PfoI T'CCnGG_A Cuts at: 2226 2325 2226 Size: 99 6595 PmeI GTTT'AAAC Cuts at: 3998 3998 Size: 6694 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3837 3869 4763 4795 6276 6308 3837 Size: 32 894 32 1481 32 4223 Fragments arranged by size: 4223 1481 894 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2199 2890 3630 3870 2199 Size: 691 740 240 5023 Fragments arranged by size: 5023 740 691 240 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6694 PsiI TTA'TAA Cuts at: 4264 4264 Size: 6694 PspOMI G'GGCC_C Cuts at: 3536 3663 3536 Size: 127 6567 PstI C_TGCA'G Cuts at: 830 3264 4046 830 Size: 2434 782 3478 Fragments arranged by size: 3478 2434 782 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6694 PvuII CAG'CTG Cuts at: 2080 2105 2387 2080 Size: 25 282 6387 Fragments arranged by size: 6387 282 25 SacI G_AGCT'C Cuts at: 3711 3855 3711 Size: 144 6550 SacII CC_GC'GG Cuts at: 2289 2289 Size: 6694 SalI G'TCGA_C Cuts at: 1936 4036 1936 Size: 2100 4594 SanDI GG'GwC_CC Cuts at: 3870 3870 Size: 6694 SapI GCTCTTCn'nnn_ Cuts at: 2661 3046 2661 Size: 385 6309 SbfI CC_TGCA'GG Cuts at: 4046 4046 Size: 6694 ScaI AGT'ACT Cuts at: 3310 4942 6654 3310 Size: 1632 1712 3350 Fragments arranged by size: 3350 1712 1632 SexAI A'CCwGG_T Cuts at: 2892 2892 Size: 6694 SfcI C'TryA_G Cuts at: 826 1045 2018 3260 3594 4042 5177 5858 6049 Size: 219 973 1242 334 448 1135 681 191 Cuts at: 6049 826 Size: 1471 Fragments arranged by size: 1471 1242 1135 973 681 448 334 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2957 2966 2957 Size: 9 6685 SfoI GGC'GCC Cuts at: 3504 3504 Size: 6694 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6694 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6694 SmaI CCC'GGG Cuts at: 1872 4021 1872 Size: 2149 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3848 4798 5699 5961 820 Size: 197 926 1905 950 901 262 1553 Fragments arranged by size: 1905 1553 950 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4485 493 Size: 3992 2702 SpeI A'CTAG_T Cuts at: 152 2453 152 Size: 2301 4393 SphI G_CATG'C Cuts at: 1132 4052 1132 Size: 2920 3774 SspI AAT'ATT Cuts at: 5 52 4618 5 Size: 47 4566 2081 Fragments arranged by size: 4566 2081 47 StuI AGG'CCT Cuts at: 1517 2489 2951 3386 1517 Size: 972 462 435 4825 Fragments arranged by size: 4825 972 462 435 StyI C'CwwG_G Cuts at: 513 1065 2004 2547 2886 3220 3605 3669 4133 Size: 552 939 543 339 334 385 64 464 Cuts at: 4133 513 Size: 3074 Fragments arranged by size: 3074 939 552 543 464 385 339 334 64 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3062 3523 4721 4738 4891 5076 5521 Size: 1255 1482 461 1198 17 153 185 445 Cuts at: 5521 325 Size: 1498 Fragments arranged by size: 1498 1482 1255 1198 461 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2148 2685 3308 Size: 274 80 33 51 1443 171 537 623 Cuts at: 3308 4940 6652 96 Size: 1632 1712 138 Fragments arranged by size: 1712 1632 1443 623 537 274 171 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6654 6686 6654 Size: 32 6662 Tth111I GACn'n_nGTC Cuts at: 1316 3799 1316 Size: 2483 4211 XbaI T'CTAG_A Cuts at: 4030 4030 Size: 6694 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2323 2893 2962 3473 2323 Size: 570 69 511 5544 Fragments arranged by size: 5544 570 511 69 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6694 XmaI C'CCGG_G Cuts at: 1870 4019 1870 Size: 2149 4545 XmnI GAAnn'nnTTC Cuts at: 2361 4823 2361 Size: 2462 4232 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4502 276 Size: 53 83 186 936 2968 2468 Fragments arranged by size: 2968 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BbvCI BfrBI BsiWI BstZ17I DraIII FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SrfI SwaI