################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI ApoI AscI AseI AsnI Asp718I AspEI AviII AvrII BbeI BcgI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrBI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII Eam1105I EcoO65I EcoRI EcoT22I FbaI FseI FspI HindIII HpaI KasI KpnI KspI MaeI MfeI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI TaqI TfiI Tsp509I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 463 GTCTAC GT^[AC][GT]AC AflIII 587 ACGCGT A^C[GA][CT]GT Aor51HI 1471 AGCGCT AGC^GCT AspI 778 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 847 CACGTG CAC^GTG BbvCI 1661 CCTCAGC CCTCAGC(-5/-2) BglII 252 AGATCT A^GATCT BsaBI 1167 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsrDI 725 GCAATG GCAATG(2/0) BssSI 256 CTCGTG CACGAG(-5/-1) BstXI 38 CCATCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 445 GACGGCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1239 CGGCCG C^GGCCG EarI 1726 GAAGAG CTCTTC(1/4) EclXI 1239 CGGCCG C^GGCCG Eco47III 1471 AGCGCT AGC^GCT Eco52I 1239 CGGCCG C^GGCCG EcoRV 935 GATATC GAT^ATC HincII 1423 GTCAAC GT[CT]^[GA]AC HindII 1423 GTCAAC GT[CT]^[GA]AC Ksp632I 1726 GAAGAG CTCTTC(1/4) MamI 1167 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 587 ACGCGT A^CGCGT MseI 659 TTAA T^TAA NcoI 596 CCATGG C^CATGG NspI 1301 GCATGT [GA]CATG^[CT] PflFI 778 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 847 CACGTG CAC^GTG PmlI 847 CACGTG CAC^GTG SapI 1726 GAAGAGC GCTCTTC(1/4) ScaI 1499 AGTACT AGT^ACT SfaNI 1001 GCATC GCATC(5/9) SfiI 855 GGCCGCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC StuI 381 AGGCCT AGG^CCT Tru9I 659 TTAA T^TAA Tth111I 778 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AocI 995 CCTCAGG CC^T[ACGT]AGG 1549 CCTCAGG ApaI 799 GGGCCC GGGCC^C 863 GGGCCC Asp700I 1497 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1590 GAACTCCTTC AvaI 513 CTCGGG C^[CT]CG[GA]G 769 CCCGAG BbsI 1369 GAAGAC GAAGAC(2/6) 754 GTCTTC BglI 856 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1766 GCCCTGCTGGC BpuAI 1369 GAAGAC GAAGAC(2/6) 754 GTCTTC BsaI 637 GAGACC GGTCTC(1/5) 1099 GAGACC BsaWI 488 TCCGGT [AT]^CCGG[AT] 570 ACCGGA BsoBI 513 CTCGGG C^[CT]CG[GA]G 769 CCCGAG BsrFI 901 GCCGGC [GA]^CCGG[CT] 1483 GCCGGC BstAPI 534 GCAGAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1695 GCAGCCCCTGC Bsu36I 995 CCTCAGG CC^T[ACGT]AGG 1549 CCTCAGG Cfr10I 901 GCCGGC [GA]^CCGG[CT] 1483 GCCGGC Eco81I 995 CCTCAGG CC^T[ACGT]AGG 1549 CCTCAGG EcoNI 435 CCTCATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1701 CCTGCCCAAGG HaeII 1031 GGCGCT [GA]GCGC^[CT] 1471 AGCGCT HinfI 808 GACTC G^A[ACGT]TC 991 GACTC NaeI 901 GCCGGC GCC^GGC 1483 GCCGGC NgoMIV 901 GCCGGC G^CCGGC 1483 GCCGGC PleI 808 GACTC GAGTC(4/5) 991 GACTC SacI 136 GAGCTC GAGCT^C 1459 GAGCTC SmlI 199 CTCAAG C^T[CT][GA]AG 241 CTTGAG XmnI 1497 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1590 GAACTCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AccII AciI AcyI AfaI AluI Alw44I AlwI AlwNI ApaLI AspHI AvaII BalI BamHI BanI BanII BbvI BcnI BmyI BpmI BsaAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfcI SspBI StyI TseI Tsp45I TspRI Van91I XhoII