Restriction map for
pFN21AE4536
(Linear) (Six-base) MAPSORT of: KIEE4536.seq Check: 3980 from: 1 to: 1809
KIEE4536.orf
With 182 enzymes: SgfI *
March 22, 2012 18:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 351 424 1552 1809
Size: 351 73 1128 257
Fragments arranged by size:
1128 351 257 73
AccI GT'mk_AC
Cuts at: 0 464 1809
Size: 464 1345
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 777 1633 1809
Size: 571 206 856 176
Fragments arranged by size:
856 571 206 176
AfeI AGC'GCT
Cuts at: 0 1473 1809
Size: 1473 336
AflIII A'CryG_T
Cuts at: 0 587 1809
Size: 587 1222
AleI CACnn'nnGTG
Cuts at: 0 31 1075 1809
Size: 31 1044 734
Fragments arranged by size:
1044 734 31
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 469 501 1809
Size: 469 32 1308
Fragments arranged by size:
1308 469 32
AlwNI CAG_nnn'CTG
Cuts at: 0 186 357 549 1701 1809
Size: 186 171 192 1152 108
Fragments arranged by size:
1152 192 186 171 108
ApaI G_GGCC'C
Cuts at: 0 803 867 1809
Size: 803 64 942
Fragments arranged by size:
942 803 64
ApaLI G'TGCA_C
Cuts at: 0 591 1137 1432 1809
Size: 591 546 295 377
Fragments arranged by size:
591 546 377 295
AvaI C'yCGr_G
Cuts at: 0 513 769 1809
Size: 513 256 1040
Fragments arranged by size:
1040 513 256
BamHI G'GATC_C
Cuts at: 0 333 470 1166 1403 1809
Size: 333 137 696 237 406
Fragments arranged by size:
696 406 333 237 137
BanI G'GyrC_C
Cuts at: 0 412 686 871 1809
Size: 412 274 185 938
Fragments arranged by size:
938 412 274 185
BanII G_rGCy'C
Cuts at: 0 76 94 140 710 803 867 1463 1733
Size: 76 18 46 570 93 64 596 270
Cuts at: 1733 1809
Size: 76
Fragments arranged by size:
596 570 270 93 76 76 64 46
18
BbsI GAAGACnn'nnnn_
Cuts at: 0 747 1376 1809
Size: 747 629 433
Fragments arranged by size:
747 629 433
BbvCI CC'TCA_GC
Cuts at: 0 1662 1809
Size: 1662 147
BglI GCCn_nnn'nGGC
Cuts at: 0 862 1772 1809
Size: 862 910 37
Fragments arranged by size:
910 862 37
BglII A'GATC_T
Cuts at: 0 252 1809
Size: 252 1557
Bme1580I G_kGCm'C
Cuts at: 0 595 803 867 1141 1436 1747 1809
Size: 595 208 64 274 295 311 62
Fragments arranged by size:
595 311 295 274 208 64 62
BmrI ACTGGGnnnn_n'
Cuts at: 0 1214 1809
Size: 1214 595
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 597 1357 1809
Size: 597 760 452
Fragments arranged by size:
760 597 452
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 324 496 517 915 1429 1477 1809
Size: 154 170 172 21 398 514 48 332
Fragments arranged by size:
514 398 332 172 170 154 48 21
Bpu10I CC'TnA_GC
Cuts at: 0 1662 1809
Size: 1662 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 262 1809
Size: 184 78 1547
Fragments arranged by size:
1547 184 78
BsaI GGTCTCn'nnnn_
Cuts at: 0 631 1093 1809
Size: 631 462 716
Fragments arranged by size:
716 631 462
BsaAI yAC'GTr
Cuts at: 0 644 849 1358 1431 1809
Size: 644 205 509 73 378
Fragments arranged by size:
644 509 378 205 73
BsaBI GATnn'nnATC
Cuts at: 0 1171 1809
Size: 1171 638
BsaHI Gr'CG_yC
Cuts at: 0 209 299 1481 1555 1809
Size: 209 90 1182 74 254
Fragments arranged by size:
1182 254 209 90 74
BsaWI w'CCGG_w
Cuts at: 0 488 570 1809
Size: 488 82 1239
Fragments arranged by size:
1239 488 82
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 469 499 628 658 1402 1432 1809
Size: 469 30 129 30 744 30 377
Fragments arranged by size:
744 469 377 129 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 493 984 1426 1809
Size: 493 491 442 383
Fragments arranged by size:
493 491 442 383
BseYI C'CCAG_C
Cuts at: 0 338 628 1056 1142 1809
Size: 338 290 428 86 667
Fragments arranged by size:
667 428 338 290 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 562 1110 1338 1524 1809
Size: 385 177 548 228 186 285
Fragments arranged by size:
548 385 285 228 186 177
BsiEI CG_ry'CG
Cuts at: 0 231 828 1242 1809
Size: 231 597 414 567
Fragments arranged by size:
597 567 414 231
BsiHKAI G_wGCw'C
Cuts at: 0 140 266 595 1141 1343 1436 1463 1809
Size: 140 126 329 546 202 93 27 346
Fragments arranged by size:
546 346 329 202 140 126 93 27
Bsp1286I G_dGCh'C
Cuts at: 0 76 94 140 266 595 710 803 867
Size: 76 18 46 126 329 115 93 64
Cuts at: 867 1141 1343 1436 1463 1733 1747 1809
Size: 274 202 93 27 270 14 62
Fragments arranged by size:
329 274 270 202 126 115 93 93
76 64 62 46 27 18 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 351 424 1552 1700 1809
Size: 351 73 1128 148 109
Fragments arranged by size:
1128 351 148 109 73
BsrDI GCAATG_nn'
Cuts at: 0 732 1809
Size: 732 1077
BsrFI r'CCGG_y
Cuts at: 0 901 1483 1809
Size: 901 582 326
Fragments arranged by size:
901 582 326
BsrGI T'GTAC_A
Cuts at: 0 20 1067 1622 1809
Size: 20 1047 555 187
Fragments arranged by size:
1047 555 187 20
BssSI C'ACGA_G
Cuts at: 0 256 1809
Size: 256 1553
BstAPI GCAn_nnn'nTGC
Cuts at: 0 540 1701 1809
Size: 540 1161 108
Fragments arranged by size:
1161 540 108
BstXI CCAn_nnnn'nTGG
Cuts at: 0 45 1809
Size: 45 1764
BstYI r'GATC_y
Cuts at: 0 252 323 333 470 623 1166 1295 1403
Size: 252 71 10 137 153 543 129 108
Cuts at: 1403 1809
Size: 406
Fragments arranged by size:
543 406 252 153 137 129 108 71
10
Bsu36I CC'TnA_GG
Cuts at: 0 996 1550 1809
Size: 996 554 259
Fragments arranged by size:
996 554 259
BtgI C'CryG_G
Cuts at: 0 231 596 1093 1809
Size: 231 365 497 716
Fragments arranged by size:
716 497 365 231
BtsI GCAGTG_nn'
Cuts at: 0 38 64 281 1150 1647 1809
Size: 38 26 217 869 497 162
Fragments arranged by size:
869 497 217 162 38 26
DrdI GACnn_nn'nnGTC
Cuts at: 0 451 1809
Size: 451 1358
EaeI y'GGCC_r
Cuts at: 0 35 59 854 1082 1239 1382 1809
Size: 35 24 795 228 157 143 427
Fragments arranged by size:
795 427 228 157 143 35 24
EagI C'GGCC_G
Cuts at: 0 1239 1809
Size: 1239 570
EarI CTCTTCn'nnn_
Cuts at: 0 1721 1809
Size: 1721 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 931 1809
Size: 931 878
EcoICRI GAG'CTC
Cuts at: 0 138 1461 1809
Size: 138 1323 348
Fragments arranged by size:
1323 348 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 324 496 517 571 777 915 1429
Size: 154 170 172 21 54 206 138 514
Cuts at: 1429 1477 1633 1809
Size: 48 156 176
Fragments arranged by size:
514 206 176 172 170 156 154 138
54 48 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 439 1705 1809
Size: 439 1266 104
Fragments arranged by size:
1266 439 104
EcoO109I rG'GnC_Cy
Cuts at: 0 128 371 404 732 800 864 926 1617
Size: 128 243 33 328 68 64 62 691
Cuts at: 1617 1809
Size: 192
Fragments arranged by size:
691 328 243 192 128 68 64 62
33
EcoRV GAT'ATC
Cuts at: 0 937 1809
Size: 937 872
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 423 922 1809
Size: 423 499 887
Fragments arranged by size:
887 499 423
HaeII r_GCGC'y
Cuts at: 0 1035 1475 1809
Size: 1035 440 334
Fragments arranged by size:
1035 440 334
HincII GTy'rAC
Cuts at: 0 1425 1809
Size: 1425 384
MluI A'CGCG_T
Cuts at: 0 587 1809
Size: 587 1222
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 1809
Size: 251 1558
MscI TGG'CCA
Cuts at: 0 37 1084 1384 1809
Size: 37 1047 300 425
Fragments arranged by size:
1047 425 300 37
MslI CAynn'nnrTG
Cuts at: 0 31 55 1075 1642 1809
Size: 31 24 1020 567 167
Fragments arranged by size:
1020 567 167 31 24
MspA1I CmG'CkG
Cuts at: 0 354 797 1146 1455 1809
Size: 354 443 349 309 354
Fragments arranged by size:
443 354 354 349 309
NaeI GCC'GGC
Cuts at: 0 903 1485 1809
Size: 903 582 324
Fragments arranged by size:
903 582 324
NcoI C'CATG_G
Cuts at: 0 596 1809
Size: 596 1213
NgoMIV G'CCGG_C
Cuts at: 0 901 1483 1809
Size: 901 582 326
Fragments arranged by size:
901 582 326
NspI r_CATG'y
Cuts at: 0 1305 1809
Size: 1305 504
PasI CC'CwG_GG
Cuts at: 0 867 1809
Size: 867 942
PflMI CCAn_nnn'nTGG
Cuts at: 0 186 647 747 852 1809
Size: 186 461 100 105 957
Fragments arranged by size:
957 461 186 105 100
PfoI T'CCnGG_A
Cuts at: 0 473 1162 1232 1289 1329 1406 1809
Size: 473 689 70 57 40 77 403
Fragments arranged by size:
689 473 403 77 70 57 40
PmlI CAC'GTG
Cuts at: 0 849 1809
Size: 849 960
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 469 501 1809
Size: 469 32 1308
Fragments arranged by size:
1308 469 32
PpuMI rG'GwC_Cy
Cuts at: 0 128 371 926 1809
Size: 128 243 555 883
Fragments arranged by size:
883 555 243 128
PspOMI G'GGCC_C
Cuts at: 0 799 863 1809
Size: 799 64 946
Fragments arranged by size:
946 799 64
PstI C_TGCA'G
Cuts at: 0 71 362 524 536 1496 1697 1809
Size: 71 291 162 12 960 201 112
Fragments arranged by size:
960 291 201 162 112 71 12
PvuII CAG'CTG
Cuts at: 0 354 1146 1455 1809
Size: 354 792 309 354
Fragments arranged by size:
792 354 354 309
SacI G_AGCT'C
Cuts at: 0 140 1463 1809
Size: 140 1323 346
Fragments arranged by size:
1323 346 140
SanDI GG'GwC_CC
Cuts at: 0 128 1809
Size: 128 1681
SapI GCTCTTCn'nnn_
Cuts at: 0 1721 1809
Size: 1721 88
ScaI AGT'ACT
Cuts at: 0 1501 1809
Size: 1501 308
SfcI C'TryA_G
Cuts at: 0 67 358 520 532 1275 1492 1693 1809
Size: 67 291 162 12 743 217 201 116
Fragments arranged by size:
743 291 217 201 162 116 67 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 862 1809
Size: 862 947
SmlI C'TyrA_G
Cuts at: 0 199 241 1809
Size: 199 42 1568
Fragments arranged by size:
1568 199 42
StuI AGG'CCT
Cuts at: 0 383 1809
Size: 383 1426
StyI C'CwwG_G
Cuts at: 0 425 596 719 875 1706 1809
Size: 425 171 123 156 831 103
Fragments arranged by size:
831 425 171 156 123 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 175 286 859 1156 1311 1809
Size: 175 111 573 297 155 498
Fragments arranged by size:
573 498 297 175 155 111
TatI w'GTAC_w
Cuts at: 0 20 1010 1067 1499 1622 1809
Size: 20 990 57 432 123 187
Fragments arranged by size:
990 432 187 123 57 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1402 1434 1566 1598 1809
Size: 1402 32 132 32 211
Fragments arranged by size:
1402 211 132 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 781 1809
Size: 781 1028
XmnI GAAnn'nnTTC
Cuts at: 0 1501 1594 1809
Size: 1501 93 215
Fragments arranged by size:
1501 215 93
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AloI AlwNI
ApaI ApaLI AvaI BamHI BanI BanII BbsI BbvCI
BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BsrGI
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FalI HaeII HincII MluI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PfoI
PmlI PpiI PpuMI PspOMI PstI PvuII SacI SanDI
SapI ScaI SfcI SfiI SmlI StuI StyI TaqII
TatI TstI Tth111I XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AlfI ApoI
AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI
BclI BfrBI BlpI BmgBI BmtI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BssHII BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DraIII EcoRI FseI FspI FspAI
Hin4I HindIII HpaI KasI KpnI MfeI NarI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI
SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XcmI XhoI XmaI ZraI