Restriction map for
pFN21AB3124
##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AccIII AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AspI AviII
AvrII BbeI BbrPI BbvCI BcgI
BciVI BfrI BglI BlnI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspEI BspLU11I BsrFI BsrGI BssHII
BstBI BstEII BstPI Bsu36I Cfr10I
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I FseI FspI HgaI HindIII
HpaI KasI KspI MfeI MluI
MroI MunI NaeI NarI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AatII 101 GACGTC GACGT^C
Acc65I 1292 GGTACC G^GTACC
AccI 576 GTATAC GT^[AC][GT]AC
AcyI 101 GACGTC G[GA]^CG[CT]C
AflIII 110 ACGTGT A^C[GA][CT]GT
AlwNI 1127 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1292 GGTACC G^GTACC
AvaI 1108 CCCGAG C^[CT]CG[GA]G
BamHI 936 GGATCC G^GATCC
BanI 1292 GGTACC G^G[CT][GA]CC
BclI 162 TGATCA T^GATCA
BcnI 771 CCGGG CC^[GC]GG
BsaBI 550 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 101 GACGTC G[GA]^CG[CT]C
BsgI 30 CTGCAC GTGCAG(16/14)
BsmI 1428 GAATGC GAATGC(1/-1)
BsoBI 1108 CCCGAG C^[CT]CG[GA]G
BspDI 943 ATCGAT AT^CGAT
BspHI 818 TCATGA T^CATGA
BsrBI 508 GAGCGG CCGCTC(-3/-3)
BsrDI 1253 GCAATG GCAATG(2/0)
Bst1107I 576 GTATAC GTA^TAC
BstAPI 1214 GCAACGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1014 CCAGGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 576 GTATAC GTA^TAC
ClaI 943 ATCGAT AT^CGAT
DraI 415 TTTAAA TTT^AAA
DraII 909 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 707 CCGTGG C^C[GA][CT]GG
EcoO109I 909 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 319 GAATTC G^AATTC
EcoRV 1462 GATATC GAT^ATC
EcoT14I 840 CCAAGG C^C[AT][AT]GG
EcoT22I 1430 ATGCAT ATGCA^T
FbaI 162 TGATCA T^GATCA
HaeII 49 AGCGCC [GA]GCGC^[CT]
HapII 771 CCGG C^CGG
Hin1I 101 GACGTC G[GA]^CG[CT]C
HincII 1354 GTTGAC GT[CT]^[GA]AC
HindII 1354 GTTGAC GT[CT]^[GA]AC
HinfI 925 GAATC G^A[ACGT]TC
HpaII 771 CCGG C^CGG
KpnI 1292 GGTACC GGTAC^C
MamI 550 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 416 TTAA T^TAA
MslI 942 CATCGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 771 CCGG C^CGG
NciI 771 CCGGG CC^[GC]GG
NdeI 380 CATATG CA^TATG
NsiI 1430 ATGCAT ATGCA^T
PpuMI 909 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 1241 CTGCAG CTGCA^G
RcaI 818 TCATGA T^CATGA
SacI 70 GAGCTC GAGCT^C
SapI 1399 GCTCTTC GCTCTTC(1/4)
SfcI 1241 CTGCAG C^T[GA][CT]AG
SpeI 1138 ACTAGT A^CTAGT
StuI 1610 AGGCCT AGG^CCT
StyI 840 CCAAGG C^C[AT][AT]GG
TfiI 925 GAATC G^A[AT]TC
Tru9I 416 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 21 CGCG CG^CG
54 CGCG
AclI 440 AACGTT AA^CGTT
1216 AACGTT
BalI 384 TGGCCA TGG^CCA
833 TGGCCA
BanII 70 GAGCTC G[GA]GC[CT]^C
494 GGGCTC
BbsI 375 GAAGAC GAAGAC(2/6)
1627 GTCTTC
BfaI 904 CTAG C^TAG
1139 CTAG
BglII 173 AGATCT A^GATCT
420 AGATCT
BlpI 45 GCTCAGC GC^T[ACGT]AGC
671 GCTTAGC
BpmI 625 CTCCAG CTGGAG(16/14)
505 CTGGAG
Bpu1102I 45 GCTCAGC GC^T[ACGT]AGC
671 GCTTAGC
BpuAI 375 GAAGAC GAAGAC(2/6)
1627 GTCTTC
BsaAI 109 TACGTG [CT]AC^GT[GA]
448 TACGTG
BsaI 702 GGTCTC GGTCTC(1/5)
775 GGTCTC
BseRI 1618 CTCCTC GAGGAG(10/8)
214 GAGGAG
BsmFI 179 GTCCC GGGAC(10/14)
607 GTCCC
BspMI 193 ACCTGC ACCTGC(4/8)
1289 GCAGGT
BstUI 21 CGCG CG^CG
54 CGCG
CelII 45 GCTCAGC GC^T[ACGT]AGC
671 GCTTAGC
EaeI 384 TGGCCA [CT]^GGCC[GA]
833 TGGCCA
FauI 19 CCCGC CCCGC(4/6)
682 GCGGG
MaeI 904 CTAG C^TAG
1139 CTAG
MluNI 384 TGGCCA TGG^CCA
833 TGGCCA
MscI 384 TGGCCA TGG^CCA
833 TGGCCA
MvnI 21 CGCG CG^CG
54 CGCG
NspI 395 ACATGC [GA]CATG^[CT]
974 ACATGC
Psp1406I 440 AACGTT AA^CGTT
1216 AACGTT
Tsp45I 781 GTGAC ^GT[GC]AC
1306 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BbvI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BssSI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI TaqI TseI Tsp509I
TspRI TthHB8I XhoII XmnI