(Circular) (Six-base) MAPSORT of: pFN21AB3124.seq Check: 7403 from: 1 to: 6373 pFN21AB3124 6373 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2110 4183 278 Size: 53 83 186 936 574 2073 2468 Fragments arranged by size: 2468 2073 936 574 186 83 53 AccI GT'mk_AC Cuts at: 1937 2582 3716 1937 Size: 645 1134 4594 Fragments arranged by size: 4594 1134 645 Acc65I G'GTAC_C Cuts at: 3297 3694 3297 Size: 397 5976 AclI AA'CG_TT Cuts at: 1563 2446 3222 4500 4873 1563 Size: 883 776 1278 373 3063 Fragments arranged by size: 3063 1278 883 776 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2615 3070 3082 3513 4436 5820 6088 1632 Size: 983 455 12 431 923 1384 268 1917 Fragments arranged by size: 1917 1384 983 923 455 431 268 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6176 AflIII A'CryG_T Cuts at: 1203 2115 5272 6249 1203 Size: 912 3157 977 1327 Fragments arranged by size: 3157 1327 977 912 AgeI A'CCGG_T Cuts at: 5204 5204 Size: 6373 AhdI GACnn_n'nnGTC Cuts at: 5102 5102 Size: 6373 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6373 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2300 1184 Size: 1116 5257 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2504 2536 2504 Size: 32 6341 AlwNI CAG_nnn'CTG Cuts at: 3137 4116 5688 3137 Size: 979 1572 3822 Fragments arranged by size: 3822 1572 979 ApaLI G'TGCA_C Cuts at: 4430 5586 4430 Size: 1156 5217 ApoI r'AATT_y Cuts at: 1493 2324 2407 3057 3197 3682 3908 4060 6169 Size: 831 83 650 140 485 226 152 2109 Cuts at: 6169 1493 Size: 1697 Fragments arranged by size: 2109 1697 831 650 485 226 152 140 83 AseI AT'TA_AT Cuts at: 160 1032 4927 160 Size: 872 3895 1606 Fragments arranged by size: 3895 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6373 AvaI C'yCGr_G Cuts at: 1870 1943 3113 3698 1870 Size: 73 1170 585 4545 Fragments arranged by size: 4545 1170 585 73 BamHI G'GATC_C Cuts at: 2941 3703 4070 2941 Size: 762 367 5244 Fragments arranged by size: 5244 762 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3297 3694 5149 618 Size: 325 217 627 1510 397 1455 1842 Fragments arranged by size: 1842 1510 1455 627 397 325 217 BanII G_rGCy'C Cuts at: 1391 2079 2503 3692 1391 Size: 688 424 1189 4072 Fragments arranged by size: 4072 1189 688 424 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2387 3625 928 Size: 971 488 1238 3676 Fragments arranged by size: 3676 1238 971 488 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4564 4598 1903 Size: 34 2627 34 3678 Fragments arranged by size: 3678 2627 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4265 5475 4265 Size: 1210 5163 BclI T'GATC_A Cuts at: 1548 2167 1548 Size: 619 5754 BfrBI ATG'CAT Cuts at: 3437 3437 Size: 6373 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4984 136 Size: 107 122 71 631 605 3312 1525 Fragments arranged by size: 3312 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2178 2425 6368 2178 Size: 247 3943 2183 Fragments arranged by size: 3943 2183 247 BlpI GC'TnA_GC Cuts at: 2051 2677 3790 2051 Size: 626 1113 4634 Fragments arranged by size: 4634 1113 626 Bme1580I G_kGCm'C Cuts at: 1790 3527 3613 4434 5590 1790 Size: 1737 86 821 1156 2573 Fragments arranged by size: 2573 1737 1156 821 86 BmgBI CAC'GTC Cuts at: 1318 3454 1318 Size: 2136 4237 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2533 2725 4104 Size: 416 53 83 399 24 1116 192 1379 Cuts at: 4104 5062 442 Size: 958 1753 Fragments arranged by size: 1753 1379 1116 958 416 399 192 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6373 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2531 2615 5033 1239 Size: 1292 84 2418 2579 Fragments arranged by size: 2579 2418 1292 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2063 2193 2655 4498 5363 5661 2063 Size: 130 462 1843 865 298 2775 Fragments arranged by size: 2775 1843 865 462 298 130 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2713 2786 5036 882 Size: 617 1214 73 2250 2219 Fragments arranged by size: 2250 2219 1214 617 73 BsaAI yAC'GTr Cuts at: 493 1204 2116 2455 4164 493 Size: 711 912 339 1709 2702 Fragments arranged by size: 2702 1709 912 711 339 BsaBI GATnn'nnATC Cuts at: 2559 6367 2559 Size: 3808 2565 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2107 4180 4562 5197 Size: 53 83 186 936 574 2073 382 635 Cuts at: 5197 275 Size: 1451 Fragments arranged by size: 2073 1451 936 635 574 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4806 5204 5478 5625 6036 4806 Size: 398 274 147 411 5143 Fragments arranged by size: 5143 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2210 2240 2504 2534 2682 Size: 30 165 30 807 30 264 30 148 Cuts at: 2682 2712 1178 Size: 30 4839 Fragments arranged by size: 4839 807 264 165 148 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2234 3614 1186 Size: 1048 1380 3945 Fragments arranged by size: 3945 1380 1048 BseYI C'CCAG_C Cuts at: 3046 3560 5576 6072 3046 Size: 514 2016 496 3347 Fragments arranged by size: 3347 2016 514 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2020 1163 Size: 573 284 5516 Fragments arranged by size: 5516 573 284 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3823 4584 5612 1540 Size: 275 186 1822 761 1028 2301 Fragments arranged by size: 2301 1822 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2052 2079 3094 4434 4519 5590 2052 Size: 27 1015 1340 85 1071 2835 Fragments arranged by size: 2835 1340 1071 1015 85 27 BsmI GAATG_Cn' Cuts at: 3439 3884 3977 3439 Size: 445 93 5835 Fragments arranged by size: 5835 445 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2079 2503 3094 3527 3613 3692 Size: 399 262 27 424 591 433 86 79 Cuts at: 3692 4434 4519 5590 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 591 433 424 399 262 86 85 79 27 BspEI T'CCGG_A Cuts at: 6036 6036 Size: 6373 BspHI T'CATG_A Cuts at: 2823 4262 5992 2823 Size: 1439 1730 3204 Fragments arranged by size: 3204 1730 1439 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2207 3285 3728 844 Size: 1363 1078 443 3489 Fragments arranged by size: 3489 1363 1078 443 BsrBI CCG'CTC Cuts at: 1394 1823 2515 4260 1394 Size: 429 692 1745 3507 Fragments arranged by size: 3507 1745 692 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3265 4868 5042 65 Size: 1183 2017 1603 174 1396 Fragments arranged by size: 2017 1603 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5017 5204 1700 Size: 110 3207 187 2869 Fragments arranged by size: 3207 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6373 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6373 BssSI C'ACGA_G Cuts at: 2153 2640 2783 4433 5445 2153 Size: 487 143 1650 1012 3081 Fragments arranged by size: 3081 1650 1012 487 143 BstAPI GCAn_nnn'nTGC Cuts at: 794 3225 794 Size: 2431 3942 BstBI TT'CG_AA Cuts at: 5177 5177 Size: 6373 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6373 BstXI CCAn_nnnn'nTGG Cuts at: 3026 3026 Size: 6373 BstYI r'GATC_y Cuts at: 1972 2178 2425 2851 2941 3703 4070 4455 4472 Size: 206 247 426 90 762 367 385 17 Cuts at: 4472 5924 6010 6022 6368 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 762 426 385 367 346 247 206 90 86 17 12 BstZ17I GTA'TAC Cuts at: 2583 2583 Size: 6373 BtgI C'CryG_G Cuts at: 513 1065 2004 2712 513 Size: 552 939 708 4174 Fragments arranged by size: 4174 939 708 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5634 Fragments arranged by size: 5634 683 56 BtsI GCAGTG_nn' Cuts at: 2052 2770 3315 3527 3889 4683 4703 2052 Size: 718 545 212 362 794 20 3722 Fragments arranged by size: 3722 794 718 545 362 212 20 ClaI AT'CG_AT Cuts at: 1554 2949 1554 Size: 1395 4978 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6338 DraI TTT'AAA Cuts at: 2422 3677 2422 Size: 1255 5118 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5380 809 Size: 507 4064 1802 Fragments arranged by size: 4064 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2389 2838 3820 Size: 54 1425 50 275 18 559 449 982 Cuts at: 3820 4709 6338 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 982 889 559 449 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3820 1537 Size: 275 2008 4090 Fragments arranged by size: 4090 2008 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2435 3411 3560 4303 1353 Size: 1082 976 149 743 3423 Fragments arranged by size: 3423 1082 976 743 149 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2513 4960 5334 5480 387 Size: 1064 138 924 2447 374 146 1280 Fragments arranged by size: 2447 1280 1064 924 374 146 138 EcoICRI GAG'CTC Cuts at: 2077 2077 Size: 6373 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2531 2615 3070 3082 3513 4436 5033 Size: 393 899 84 455 12 431 923 597 Cuts at: 5033 5820 6088 1239 Size: 787 268 1524 Fragments arranged by size: 1524 923 899 787 597 455 431 393 268 84 12 EcoO109I rG'GnC_Cy Cuts at: 2915 2915 Size: 6373 EcoRI G'AATT_C Cuts at: 2324 3682 4060 2324 Size: 1358 378 4637 Fragments arranged by size: 4637 1358 378 EcoRV GAT'ATC Cuts at: 3469 3469 Size: 6373 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2357 2357 Size: 6373 FspI TGC'GCA Cuts at: 4879 4879 Size: 6373 HaeII r_GCGC'y Cuts at: 1678 2058 5520 1678 Size: 380 3462 2531 Fragments arranged by size: 3462 2531 380 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2066 2098 3376 3408 5014 Size: 32 135 32 494 32 1278 32 1606 Cuts at: 5014 5046 5088 5120 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1606 1278 494 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3361 3717 3963 669 Size: 988 281 1423 356 246 3079 Fragments arranged by size: 3079 1423 988 356 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6373 HpaI GTT'AAC Cuts at: 3963 3963 Size: 6373 KpnI G_GTAC'C Cuts at: 3301 3698 3301 Size: 397 5976 MfeI C'AATT_G Cuts at: 3972 3972 Size: 6373 MluI A'CGCG_T Cuts at: 6249 6249 Size: 6373 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3254 5487 5671 1305 Size: 1949 2233 184 2007 Fragments arranged by size: 2233 2007 1949 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2391 2840 10 Size: 54 1425 343 559 449 3543 Fragments arranged by size: 3543 1425 559 449 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2951 4331 4690 4849 518 Size: 648 1785 1380 359 159 2042 Fragments arranged by size: 2042 1785 1380 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2593 3050 3137 3789 4466 5614 5859 1606 Size: 987 457 87 652 677 1148 245 2120 Fragments arranged by size: 2120 1148 987 677 652 457 245 87 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4882 Fragments arranged by size: 4882 939 552 NdeI CA'TA_TG Cuts at: 387 2386 387 Size: 1999 4374 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6373 NotI GC'GGCC_GC Cuts at: 3820 3820 Size: 6373 NsiI A_TGCA'T Cuts at: 3439 3439 Size: 6373 NspI r_CATG'y Cuts at: 1132 1229 2404 2983 3731 5276 1132 Size: 97 1175 579 748 1545 2229 Fragments arranged by size: 2229 1545 1175 748 579 97 PasI CC'CwG_GG Cuts at: 1348 2188 1348 Size: 840 5533 PciI A'CATG_T Cuts at: 5272 5272 Size: 6373 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6373 PfoI T'CCnGG_A Cuts at: 2508 2508 Size: 6373 PmeI GTTT'AAAC Cuts at: 3677 3677 Size: 6373 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2504 2536 4442 4474 5955 5987 2504 Size: 32 1906 32 1481 32 2890 Fragments arranged by size: 2890 1906 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2915 2915 Size: 6373 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6373 PsiI TTA'TAA Cuts at: 3943 3943 Size: 6373 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2525 2557 2525 Size: 32 6341 PstI C_TGCA'G Cuts at: 830 3250 3725 830 Size: 2420 475 3478 Fragments arranged by size: 3478 2420 475 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6373 PvuII CAG'CTG Cuts at: 2593 3050 3137 2593 Size: 457 87 5829 Fragments arranged by size: 5829 457 87 SacI G_AGCT'C Cuts at: 2079 2079 Size: 6373 SalI G'TCGA_C Cuts at: 1936 3715 1936 Size: 1779 4594 SapI GCTCTTCn'nnn_ Cuts at: 3411 3411 Size: 6373 SbfI CC_TGCA'GG Cuts at: 3725 3725 Size: 6373 ScaI AGT'ACT Cuts at: 4621 6333 4621 Size: 1712 4661 SfcI C'TryA_G Cuts at: 826 1045 3246 3721 4856 5537 5728 826 Size: 219 2201 475 1135 681 191 1471 Fragments arranged by size: 2201 1471 1135 681 475 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6373 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6373 SmaI CCC'GGG Cuts at: 1872 3700 1872 Size: 1828 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2078 2208 2670 4477 5378 5640 Size: 197 926 135 130 462 1807 901 262 Cuts at: 5640 820 Size: 1553 Fragments arranged by size: 1807 1553 926 901 462 262 197 135 130 SnaBI TAC'GTA Cuts at: 493 4164 493 Size: 3671 2702 SpeI A'CTAG_T Cuts at: 152 3143 152 Size: 2991 3382 SphI G_CATG'C Cuts at: 1132 3731 1132 Size: 2599 3774 SspI AAT'ATT Cuts at: 5 52 4297 5 Size: 47 4245 2081 Fragments arranged by size: 4245 2081 47 StuI AGG'CCT Cuts at: 1517 3617 1517 Size: 2100 4273 StyI C'CwwG_G Cuts at: 513 1065 2004 2845 3812 513 Size: 552 939 841 967 3074 Fragments arranged by size: 3074 967 939 841 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2923 3168 4400 4417 4570 4755 5200 Size: 1255 1343 245 1232 17 153 185 445 Cuts at: 5200 325 Size: 1498 Fragments arranged by size: 1498 1343 1255 1232 445 245 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2226 4619 6331 Size: 274 80 33 51 1443 249 2393 1712 Cuts at: 6331 96 Size: 138 Fragments arranged by size: 2393 1712 1443 274 249 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2682 2714 6333 6365 2682 Size: 32 3619 32 2690 Fragments arranged by size: 3619 2690 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6373 XbaI T'CTAG_A Cuts at: 3709 3709 Size: 6373 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6373 XmaI C'CCGG_G Cuts at: 1870 3698 1870 Size: 1828 4545 XmnI GAAnn'nnTTC Cuts at: 2600 2873 3014 4502 2600 Size: 273 141 1488 4471 Fragments arranged by size: 4471 1488 273 141 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2108 4181 276 Size: 53 83 186 936 574 2073 2468 Fragments arranged by size: 2468 2073 936 574 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI