Restriction map for
pFN21AB3124
(Linear) (Six-base) MAPSORT of: KIBB3124.seq Check: 7941 from: 1 to: 1668
KIBB3124.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AatII G_ACGT'C
Cuts at: 0 105 1668
Size: 105 1563
AccI GT'mk_AC
Cuts at: 0 577 1668
Size: 577 1091
Acc65I G'GTAC_C
Cuts at: 0 1292 1668
Size: 1292 376
AclI AA'CG_TT
Cuts at: 0 441 1217 1668
Size: 441 776 451
Fragments arranged by size:
776 451 441
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 1065 1077 1508 1668
Size: 610 455 12 431 160
Fragments arranged by size:
610 455 431 160 12
AflIII A'CryG_T
Cuts at: 0 110 1668
Size: 110 1558
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 295 1668
Size: 295 1373
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 499 531 1668
Size: 499 32 1137
Fragments arranged by size:
1137 499 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1132 1668
Size: 1132 536
ApoI r'AATT_y
Cuts at: 0 319 402 1052 1192 1668
Size: 319 83 650 140 476
Fragments arranged by size:
650 476 319 140 83
AvaI C'yCGr_G
Cuts at: 0 1108 1668
Size: 1108 560
BamHI G'GATC_C
Cuts at: 0 936 1668
Size: 936 732
BanI G'GyrC_C
Cuts at: 0 1292 1668
Size: 1292 376
BanII G_rGCy'C
Cuts at: 0 74 498 1668
Size: 74 424 1170
Fragments arranged by size:
1170 424 74
BbsI GAAGACnn'nnnn_
Cuts at: 0 382 1620 1668
Size: 382 1238 48
Fragments arranged by size:
1238 382 48
BclI T'GATC_A
Cuts at: 0 162 1668
Size: 162 1506
BfrBI ATG'CAT
Cuts at: 0 1432 1668
Size: 1432 236
BglII A'GATC_T
Cuts at: 0 173 420 1668
Size: 173 247 1248
Fragments arranged by size:
1248 247 173
BlpI GC'TnA_GC
Cuts at: 0 46 672 1668
Size: 46 626 996
Fragments arranged by size:
996 626 46
Bme1580I G_kGCm'C
Cuts at: 0 1522 1608 1668
Size: 1522 86 60
Fragments arranged by size:
1522 86 60
BmgBI CAC'GTC
Cuts at: 0 1449 1668
Size: 1449 219
BmrI ACTGGGnnnn_n'
Cuts at: 0 528 720 1668
Size: 528 192 948
Fragments arranged by size:
948 528 192
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 610 1668
Size: 526 84 1058
Fragments arranged by size:
1058 526 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 188 650 1668
Size: 58 130 462 1018
Fragments arranged by size:
1018 462 130 58
BsaI GGTCTCn'nnnn_
Cuts at: 0 708 781 1668
Size: 708 73 887
Fragments arranged by size:
887 708 73
BsaAI yAC'GTr
Cuts at: 0 111 450 1668
Size: 111 339 1218
Fragments arranged by size:
1218 339 111
BsaBI GATnn'nnATC
Cuts at: 0 554 1668
Size: 554 1114
BsaHI Gr'CG_yC
Cuts at: 0 102 1668
Size: 102 1566
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 205 235 499 529 677 707 1668
Size: 205 30 264 30 148 30 961
Fragments arranged by size:
961 264 205 148 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 229 1609 1668
Size: 229 1380 59
Fragments arranged by size:
1380 229 59
BseYI C'CCAG_C
Cuts at: 0 1041 1555 1668
Size: 1041 514 113
Fragments arranged by size:
1041 514 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 1668
Size: 15 1653
BsiHKAI G_wGCw'C
Cuts at: 0 47 74 1089 1668
Size: 47 27 1015 579
Fragments arranged by size:
1015 579 47 27
BsmI GAATG_Cn'
Cuts at: 0 1434 1668
Size: 1434 234
Bsp1286I G_dGCh'C
Cuts at: 0 47 74 498 1089 1522 1608 1668
Size: 47 27 424 591 433 86 60
Fragments arranged by size:
591 433 424 86 60 47 27
BspHI T'CATG_A
Cuts at: 0 818 1668
Size: 818 850
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 202 1280 1668
Size: 202 1078 388
Fragments arranged by size:
1078 388 202
BsrBI CCG'CTC
Cuts at: 0 510 1668
Size: 510 1158
BsrDI GCAATG_nn'
Cuts at: 0 1260 1668
Size: 1260 408
BssSI C'ACGA_G
Cuts at: 0 148 635 778 1668
Size: 148 487 143 890
Fragments arranged by size:
890 487 148 143
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1220 1668
Size: 1220 448
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1021 1668
Size: 1021 647
BstYI r'GATC_y
Cuts at: 0 173 420 846 936 1668
Size: 173 247 426 90 732
Fragments arranged by size:
732 426 247 173 90
BstZ17I GTA'TAC
Cuts at: 0 578 1668
Size: 578 1090
BtgI C'CryG_G
Cuts at: 0 707 1668
Size: 707 961
BtsI GCAGTG_nn'
Cuts at: 0 47 765 1310 1522 1668
Size: 47 718 545 212 146
Fragments arranged by size:
718 545 212 146 47
ClaI AT'CG_AT
Cuts at: 0 944 1668
Size: 944 724
DraI TTT'AAA
Cuts at: 0 417 1668
Size: 417 1251
EaeI y'GGCC_r
Cuts at: 0 384 833 1668
Size: 384 449 835
Fragments arranged by size:
835 449 384
EarI CTCTTCn'nnn_
Cuts at: 0 430 1406 1555 1668
Size: 430 976 149 113
Fragments arranged by size:
976 430 149 113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 508 1668
Size: 508 1160
EcoICRI GAG'CTC
Cuts at: 0 72 1668
Size: 72 1596
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 610 1065 1077 1508 1668
Size: 526 84 455 12 431 160
Fragments arranged by size:
526 455 431 160 84 12
EcoO109I rG'GnC_Cy
Cuts at: 0 910 1668
Size: 910 758
EcoRI G'AATT_C
Cuts at: 0 319 1668
Size: 319 1349
EcoRV GAT'ATC
Cuts at: 0 1464 1668
Size: 1464 204
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 352 1668
Size: 352 1316
HaeII r_GCGC'y
Cuts at: 0 53 1668
Size: 53 1615
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 61 93 1371 1403 1668
Size: 61 32 1278 32 265
Fragments arranged by size:
1278 265 61 32 32
HincII GTy'rAC
Cuts at: 0 1356 1668
Size: 1356 312
KpnI G_GTAC'C
Cuts at: 0 1296 1668
Size: 1296 372
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1249 1668
Size: 1249 419
MscI TGG'CCA
Cuts at: 0 386 835 1668
Size: 386 449 833
Fragments arranged by size:
833 449 386
MslI CAynn'nnrTG
Cuts at: 0 946 1668
Size: 946 722
MspA1I CmG'CkG
Cuts at: 0 588 1045 1132 1668
Size: 588 457 87 536
Fragments arranged by size:
588 536 457 87
NdeI CA'TA_TG
Cuts at: 0 381 1668
Size: 381 1287
NsiI A_TGCA'T
Cuts at: 0 1434 1668
Size: 1434 234
NspI r_CATG'y
Cuts at: 0 399 978 1668
Size: 399 579 690
Fragments arranged by size:
690 579 399
PasI CC'CwG_GG
Cuts at: 0 183 1668
Size: 183 1485
PfoI T'CCnGG_A
Cuts at: 0 503 1668
Size: 503 1165
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 499 531 1668
Size: 499 32 1137
Fragments arranged by size:
1137 499 32
PpuMI rG'GwC_Cy
Cuts at: 0 910 1668
Size: 910 758
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 520 552 1668
Size: 520 32 1116
Fragments arranged by size:
1116 520 32
PstI C_TGCA'G
Cuts at: 0 1245 1668
Size: 1245 423
PvuII CAG'CTG
Cuts at: 0 588 1045 1132 1668
Size: 588 457 87 536
Fragments arranged by size:
588 536 457 87
SacI G_AGCT'C
Cuts at: 0 74 1668
Size: 74 1594
SapI GCTCTTCn'nnn_
Cuts at: 0 1406 1668
Size: 1406 262
SfcI C'TryA_G
Cuts at: 0 1241 1668
Size: 1241 427
SmlI C'TyrA_G
Cuts at: 0 73 203 665 1668
Size: 73 130 462 1003
Fragments arranged by size:
1003 462 130 73
SpeI A'CTAG_T
Cuts at: 0 1138 1668
Size: 1138 530
StuI AGG'CCT
Cuts at: 0 1612 1668
Size: 1612 56
StyI C'CwwG_G
Cuts at: 0 840 1668
Size: 840 828
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 918 1163 1668
Size: 918 245 505
Fragments arranged by size:
918 505 245
TatI w'GTAC_w
Cuts at: 0 221 1668
Size: 221 1447
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 677 709 1668
Size: 677 32 959
Fragments arranged by size:
959 677 32
XmnI GAAnn'nnTTC
Cuts at: 0 595 868 1009 1668
Size: 595 273 141 659
Fragments arranged by size:
659 595 273 141
ZraI GAC'GTC
Cuts at: 0 103 1668
Size: 103 1565
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AlfI AloI
AlwNI ApoI AvaI BamHI BanI BanII BbsI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BssSI
BstAPI BstXI BstYI BstZ17I BtgI BtsI ClaI DraI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HincII KpnI MmeI MscI MslI
MspA1I NdeI NsiI NspI PasI PfoI PpiI PpuMI
PsrI PstI PvuII SacI SapI SfcI SmlI SpeI
StuI StyI TaqII TatI TstI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AleI ApaI ApaLI
AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI
BciVI BglI BmtI BplI Bpu10I BsaWI BsiEI BsiWI
BsmBI BspEI BsrFI BsrGI BssHII BstBI BstEII Bsu36I
BtgZI CspCI DraIII DrdI EagI EcoNI FseI FspI
FspAI HindIII HpaI KasI MfeI MluI NaeI NarI
NcoI NgoMIV NheI NotI NruI PacI PciI PflMI
PmeI PmlI PshAI PsiI PspOMI PspXI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SphI SrfI SspI SwaI
Tth111I XbaI XcmI XhoI XmaI