Restriction map for
pFN21AE0922
(Circular) (Six-base) MAPSORT of: pFN21AE0922.seq Check: 5075 from: 1 to: 6724
pFN21AE0922 6724 bp
With 182 enzymes: SgfI *
April 15, 2010 09:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2290 3004 2290
Size: 714 6010
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4534 278
Size: 53 83 186 936 2998 2468
Fragments arranged by size:
2998 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4067 1937
Size: 2130 4594
Acc65I G'GTAC_C
Cuts at: 4045 4045
Size: 6724
AclI AA'CG_TT
Cuts at: 1563 4851 5224 1563
Size: 3288 373 3063
Fragments arranged by size:
3288 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2681 2978 3083 3275 3478 4010 4787 6171
Size: 1049 297 105 192 203 532 777 1384
Cuts at: 6171 6439 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1049 777 532 297 268 203
192 105
AfeI AGC'GCT
Cuts at: 3117 3117
Size: 6724
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6527
AflIII A'CryG_T
Cuts at: 1203 3638 5623 6600 1203
Size: 2435 1985 977 1327
Fragments arranged by size:
2435 1985 1327 977
AgeI A'CCGG_T
Cuts at: 5555 5555
Size: 6724
AhdI GACnn_n'nnGTC
Cuts at: 3256 5453 3256
Size: 2197 4527
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6724
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6724
AlwNI CAG_nnn'CTG
Cuts at: 4467 6039 4467
Size: 1572 5152
ApaLI G'TGCA_C
Cuts at: 4781 5937 4781
Size: 1156 5568
ApoI r'AATT_y
Cuts at: 1493 3923 4033 4259 4411 6520 1493
Size: 2430 110 226 152 2109 1697
Fragments arranged by size:
2430 2109 1697 226 152 110
AseI AT'TA_AT
Cuts at: 160 1032 5278 160
Size: 872 4246 1606
Fragments arranged by size:
4246 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6724
AvaI C'yCGr_G
Cuts at: 1870 1943 2320 2811 3051 3140 4049 1870
Size: 73 377 491 240 89 909 4545
Fragments arranged by size:
4545 909 491 377 240 89 73
BamHI G'GATC_C
Cuts at: 3501 4054 4421 3501
Size: 553 367 5804
Fragments arranged by size:
5804 553 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2024 4045 5500 618
Size: 325 217 627 237 2021 1455 1842
Fragments arranged by size:
2021 1842 1455 627 325 237 217
BanII G_rGCy'C
Cuts at: 1391 2042 3300 3476 4043 1391
Size: 651 1258 176 567 4072
Fragments arranged by size:
4072 1258 651 567 176
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2669 2954 3159 3682 3869 928
Size: 971 770 285 205 523 187 3783
Fragments arranged by size:
3783 971 770 523 285 205 187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2618 2652 4915 4949 1903
Size: 34 681 34 2263 34 3678
Fragments arranged by size:
3678 2263 681 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2759 3895 4616 5826 2759
Size: 1136 721 1210 3657
Fragments arranged by size:
3657 1210 1136 721
BclI T'GATC_A
Cuts at: 1548 2916 1548
Size: 1368 5356
BfrBI ATG'CAT
Cuts at: 3770 3770
Size: 6724
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3578 4001 5335
Size: 107 122 71 631 605 1906 423 1334
Cuts at: 5335 136
Size: 1525
Fragments arranged by size:
1906 1525 1334 631 605 423 122 107
71
BglII A'GATC_T
Cuts at: 2355 2877 3264 3588 6719 2355
Size: 522 387 324 3131 2360
Fragments arranged by size:
3131 2360 522 387 324
BlpI GC'TnA_GC
Cuts at: 4141 4141
Size: 6724
Bme1580I G_kGCm'C
Cuts at: 1790 4785 5941 1790
Size: 2995 1156 2573
Fragments arranged by size:
2995 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3724 1318
Size: 2406 4318
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2590 4455 5413
Size: 416 53 83 399 24 1173 1865 958
Cuts at: 5413 442
Size: 1753
Fragments arranged by size:
1865 1753 1173 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6724
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2450 2474 3291 3479 5384 1239
Size: 1211 24 817 188 1905 2579
Fragments arranged by size:
2579 1905 1211 817 188 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4849 5714 6012 4849
Size: 865 298 5561
Fragments arranged by size:
5561 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2845 3267 5387 882
Size: 617 1346 422 2120 2219
Fragments arranged by size:
2219 2120 1346 617 422
BsaAI yAC'GTr
Cuts at: 493 1204 4515 493
Size: 711 3311 2702
Fragments arranged by size:
3311 2702 711
BsaBI GATnn'nnATC
Cuts at: 2655 2831 6718 2655
Size: 176 3887 2661
Fragments arranged by size:
3887 2661 176
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4531 4913 5548 275
Size: 53 83 186 936 2998 382 635 1451
Fragments arranged by size:
2998 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2751 2883 3681 3915 3977 5157 5555 5829 5976
Size: 132 798 234 62 1180 398 274 147
Cuts at: 5976 6387 2751
Size: 411 3088
Fragments arranged by size:
3088 1180 798 411 398 274 234 147
132 62
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2024 2054 1178
Size: 30 165 30 621 30 5848
Fragments arranged by size:
5848 621 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2519 3548 3555 1186
Size: 1333 1029 7 4355
Fragments arranged by size:
4355 1333 1029 7
BseYI C'CCAG_C
Cuts at: 3079 5927 6423 3079
Size: 2848 496 3380
Fragments arranged by size:
3380 2848 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3063 1163
Size: 573 1327 4824
Fragments arranged by size:
4824 1327 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4174 4935 5963 1540
Size: 275 186 2173 761 1028 2301
Fragments arranged by size:
2301 2173 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2759 3476 3732 4785 4870 5941 2759
Size: 717 256 1053 85 1071 3542
Fragments arranged by size:
3542 1071 1053 717 256 85
BsmI GAATG_Cn'
Cuts at: 4235 4328 4235
Size: 93 6631
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2759 3300 3476 3732 4043 4785
Size: 399 252 717 541 176 256 311 742
Cuts at: 4785 4870 5941 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 717 541 399 311 256
252 176 85
BspEI T'CCGG_A
Cuts at: 2751 2883 3681 3915 3977 6387 2751
Size: 132 798 234 62 2410 3088
Fragments arranged by size:
3088 2410 798 234 132 62
BspHI T'CATG_A
Cuts at: 3300 3645 4613 6343 3300
Size: 345 968 1730 3681
Fragments arranged by size:
3681 1730 968 345
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2290 3004 3912 4079 844
Size: 1446 714 908 167 3489
Fragments arranged by size:
3489 1446 908 714 167
BsrBI CCG'CTC
Cuts at: 1394 1823 2422 4611 1394
Size: 429 599 2189 3507
Fragments arranged by size:
3507 2189 599 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2214 3193 5219 5393 65
Size: 1183 966 979 2026 174 1396
Fragments arranged by size:
2026 1396 1183 979 966 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3310 3356 5368 5555 1700
Size: 110 1500 46 2012 187 2869
Fragments arranged by size:
2869 2012 1500 187 110 46
BsrGI T'GTAC_A
Cuts at: 96 2091 96
Size: 1995 4729
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6724
BssSI C'ACGA_G
Cuts at: 4784 5796 4784
Size: 1012 5712
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2024 794
Size: 1230 5494
BstBI TT'CG_AA
Cuts at: 5528 5528
Size: 6724
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6724
BstXI CCAn_nnnn'nTGG
Cuts at: 3880 3880
Size: 6724
BstYI r'GATC_y
Cuts at: 1972 2355 2877 3204 3264 3501 3588 4054 4421
Size: 383 522 327 60 237 87 466 367
Cuts at: 4421 4806 4823 6275 6361 6373 6719 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 522 466 385 383 367 346
327 237 87 86 60 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3345 3519 513
Size: 552 939 1341 174 3718
Fragments arranged by size:
3718 1341 939 552 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5985
Fragments arranged by size:
5985 683 56
BtsI GCAGTG_nn'
Cuts at: 2094 2122 3051 3898 4240 5034 5054 2094
Size: 28 929 847 342 794 20 3764
Fragments arranged by size:
3764 929 847 794 342 28 20
ClaI AT'CG_AT
Cuts at: 1554 3165 1554
Size: 1611 5113
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6689
DraI TTT'AAA
Cuts at: 2226 4028 2226
Size: 1802 4922
DraIII CAC_nnn'GTG
Cuts at: 2024 3614 2024
Size: 1590 5134
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3220 5731 809
Size: 507 1904 2511 1802
Fragments arranged by size:
2511 1904 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2458 2733
Size: 54 1425 50 275 18 177 451 275
Cuts at: 2733 2989 3232 3390 3487 3516 3777 4171 5060
Size: 256 243 158 97 29 261 394 889
Cuts at: 5060 6689 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 451 394 275 275 261
256 243 177 158 97 54 50 43
29 18
EagI C'GGCC_G
Cuts at: 1537 1812 4171 1537
Size: 275 2359 4090
Fragments arranged by size:
4090 2359 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2789 3815 4654 1353
Size: 1436 1026 839 3423
Fragments arranged by size:
3423 1436 1026 839
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5311 5685 5831 387
Size: 1064 138 3722 374 146 1280
Fragments arranged by size:
3722 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3474 3474
Size: 6724
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2450 2474 2681 2978 3083 3275 3291
Size: 393 818 24 207 297 105 192 16
Cuts at: 3291 3478 3479 4010 4787 5384 6171 6439 1239
Size: 187 1 531 777 597 787 268 1524
Fragments arranged by size:
1524 818 787 777 597 531 393 297
268 207 192 187 105 24 16 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2489 2558 3554 2489
Size: 69 996 5659
Fragments arranged by size:
5659 996 69
EcoO109I rG'GnC_Cy
Cuts at: 2209 2425 2527 2551 2800 2209
Size: 216 102 24 249 6133
Fragments arranged by size:
6133 249 216 102 24
EcoRI G'AATT_C
Cuts at: 3923 4033 4411 3923
Size: 110 378 6236
Fragments arranged by size:
6236 378 110
EcoRV GAT'ATC
Cuts at: 3169 3169
Size: 6724
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3244 3826 3244
Size: 582 6142
FspI TGC'GCA
Cuts at: 5230 5230
Size: 6724
HaeII r_GCGC'y
Cuts at: 1678 3119 5871 1678
Size: 1441 2752 2531
Fragments arranged by size:
2752 2531 1441
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2303 2335 3242 3274 5365
Size: 32 135 32 731 32 907 32 2091
Cuts at: 5365 5397 5439 5471 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2091 907 731 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4068 4314 669
Size: 988 281 2130 246 3079
Fragments arranged by size:
3079 2130 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6724
HpaI GTT'AAC
Cuts at: 4314 4314
Size: 6724
KpnI G_GTAC'C
Cuts at: 4049 4049
Size: 6724
MfeI C'AATT_G
Cuts at: 4323 4323
Size: 6724
MluI A'CGCG_T
Cuts at: 6600 6600
Size: 6724
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2835 5838 6022 1305
Size: 1530 3003 184 2007
Fragments arranged by size:
3003 2007 1530 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2460 2991 3392 3489
Size: 54 1425 343 177 451 531 401 97
Cuts at: 3489 3518 3779 10
Size: 29 261 2955
Fragments arranged by size:
2955 1425 531 451 401 343 261 177
97 54 29
MslI CAynn'nnrTG
Cuts at: 518 1166 2099 3321 3707 4682 5041 5200 518
Size: 648 933 1222 386 975 359 159 2042
Fragments arranged by size:
2042 1222 975 933 648 386 359 159
MspA1I CmG'CkG
Cuts at: 1606 2392 3196 4140 4817 5965 6210 1606
Size: 786 804 944 677 1148 245 2120
Fragments arranged by size:
2120 1148 944 804 786 677 245
NaeI GCC'GGC
Cuts at: 3358 3358
Size: 6724
NcoI C'CATG_G
Cuts at: 513 1065 2004 3345 513
Size: 552 939 1341 3892
Fragments arranged by size:
3892 1341 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6724
NgoMIV G'CCGG_C
Cuts at: 3356 3356
Size: 6724
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6724
NotI GC'GGCC_GC
Cuts at: 4171 4171
Size: 6724
NsiI A_TGCA'T
Cuts at: 3772 3772
Size: 6724
NspI r_CATG'y
Cuts at: 1132 1229 2863 4082 5627 1132
Size: 97 1634 1219 1545 2229
Fragments arranged by size:
2229 1634 1545 1219 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6724
PciI A'CATG_T
Cuts at: 5623 5623
Size: 6724
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2395 1108
Size: 1287 5437
PfoI T'CCnGG_A
Cuts at: 2366 2547 3000 3693 2366
Size: 181 453 693 5397
Fragments arranged by size:
5397 693 453 181
PmeI GTTT'AAAC
Cuts at: 4028 4028
Size: 6724
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4793 4825 6306 6338 4793
Size: 32 1481 32 5179
Fragments arranged by size:
5179 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2209 2425 2527 2551 2209
Size: 216 102 24 6382
Fragments arranged by size:
6382 216 102 24
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6724
PsiI TTA'TAA
Cuts at: 4294 4294
Size: 6724
PstI C_TGCA'G
Cuts at: 830 4076 830
Size: 3246 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6724
PvuII CAG'CTG
Cuts at: 2392 2392
Size: 6724
SacI G_AGCT'C
Cuts at: 3476 3476
Size: 6724
SalI G'TCGA_C
Cuts at: 1936 4066 1936
Size: 2130 4594
SapI GCTCTTCn'nnn_
Cuts at: 2789 2789
Size: 6724
SbfI CC_TGCA'GG
Cuts at: 4076 4076
Size: 6724
ScaI AGT'ACT
Cuts at: 4972 6684 4972
Size: 1712 5012
SexAI A'CCwGG_T
Cuts at: 2529 2529
Size: 6724
SfcI C'TryA_G
Cuts at: 826 1045 2908 4072 5207 5888 6079 826
Size: 219 1863 1164 1135 681 191 1471
Fragments arranged by size:
1863 1471 1164 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6724
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6724
SmaI CCC'GGG
Cuts at: 1872 4051 1872
Size: 2179 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4828 5729 5991 820
Size: 197 926 2885 901 262 1553
Fragments arranged by size:
2885 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4515 493
Size: 4022 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6724
SphI G_CATG'C
Cuts at: 1132 4082 1132
Size: 2950 3774
SspI AAT'ATT
Cuts at: 5 52 2768 4648 5
Size: 47 2716 1880 2081
Fragments arranged by size:
2716 2081 1880 47
StuI AGG'CCT
Cuts at: 1517 3431 3995 1517
Size: 1914 564 4246
Fragments arranged by size:
4246 1914 564
StyI C'CwwG_G
Cuts at: 513 1065 2004 3345 3630 4163 513
Size: 552 939 1341 285 533 3074
Fragments arranged by size:
3074 1341 939 552 533 285
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4751 4768 4921 5106 5551 325
Size: 1255 3171 17 153 185 445 1498
Fragments arranged by size:
3171 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2091 2940 4970
Size: 274 80 33 51 1443 114 849 2030
Cuts at: 4970 6682 96
Size: 1712 138
Fragments arranged by size:
2030 1712 1443 849 274 138 114 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6684 6716 6684
Size: 32 6692
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6724
XbaI T'CTAG_A
Cuts at: 4060 4060
Size: 6724
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3721 3721
Size: 6724
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6724
XmaI C'CCGG_G
Cuts at: 1870 4049 1870
Size: 2179 4545
XmnI GAAnn'nnTTC
Cuts at: 4853 4853
Size: 6724
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4532 276
Size: 53 83 186 936 2998 2468
Fragments arranged by size:
2998 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I FseI FspAI KasI
NarI NruI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SfiI SfoI SrfI SwaI