Restriction map for
pFN21AE1411
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BanI BbeI BbrPI BbsI
BbvCI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspHI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI EaeI EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MscI MslI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SspI SwaI
TaqI Tsp509I TspRI Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 152 TCCGGA T^CCGGA
AflIII 587 ACATGT A^C[GA][CT]GT
Aor51HI 506 AGCGCT AGC^GCT
AvaII 567 GGACC G^G[AT]CC
BamHI 314 GGATCC G^GATCC
BcgI 285 CGACCTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 217 CTAG C^TAG
BpmI 40 CTGGAG CTGGAG(16/14)
BsaWI 152 TCCGGA [AT]^CCGG[AT]
BseAI 152 TCCGGA T^CCGGA
BspEI 152 TCCGGA T^CCGGA
BspLU11I 587 ACATGT A^CATGT
BsrBI 27 CCGCTC CCGCTC(-3/-3)
BsrFI 350 ACCGGC [GA]^CCGG[CT]
BstYI 314 GGATCC [GA]^GATC[CT]
CfoI 507 GCGC GCG^C
Cfr10I 350 ACCGGC [GA]^CCGG[CT]
DdeI 386 CTGAG C^T[ACGT]AG
DpnII 315 GATC ^GATC
DraIII 460 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 398 CCATGG C^C[GA][CT]GG
EarI 271 GAAGAG CTCTTC(1/4)
Eco47III 506 AGCGCT AGC^GCT
EcoT14I 398 CCATGG C^C[AT][AT]GG
FauI 552 GCGGG CCCGC(4/6)
HaeII 506 AGCGCT [GA]GCGC^[CT]
HhaI 507 GCGC GCG^C
HinP1I 507 GCGC G^CGC
HphI 298 GGTGA GGTGA(8/7)
Ksp632I 271 GAAGAG CTCTTC(1/4)
MaeI 217 CTAG C^TAG
MaeII 464 ACGT A^CGT
MaeIII 299 GTGAC ^GT[ACGT]AC
MboI 315 GATC ^GATC
MflI 314 GGATCC [GA]^GATC[CT]
MroI 152 TCCGGA T^CCGGA
MseI 359 TTAA T^TAA
NcoI 398 CCATGG C^CATGG
NdeII 315 GATC ^GATC
NspI 587 ACATGT [GA]CATG^[CT]
PflMI 372 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 293 CTGCAG CTGCA^G
PvuII 364 CAGCTG CAG^CTG
Sau3AI 315 GATC ^GATC
SbfI 292 CCTGCAGG CCTGCA^GG
ScaI 21 AGTACT AGT^ACT
Sse8387I 292 CCTGCAGG CCTGCA^GG
StyI 398 CCATGG C^C[AT][AT]GG
Tru9I 359 TTAA T^TAA
Tsp45I 299 GTGAC ^GT[GC]AC
Van91I 372 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 314 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AfaI 22 GTAC GT^AC
84 GTAC
AlwI 315 GATCC GGATC(4/5)
314 GGATC
AlwNI 373 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
532 CAGAGCCTG
ApaI 192 GGGCCC GGGCC^C
381 GGGCCC
BsmAI 11 GAGAC GTCTC(1/5)
408 GTCTC
BsrI 72 ACTGG ACTGG(1/-1)
310 ACTGG
BstF5I 327 CATCC GGATG(2/0)
492 CATCC
EcoNI 87 CCTCTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
239 CCTGGGTCAGG
EcoRV 175 GATATC GAT^ATC
430 GATATC
FokI 327 CATCC GGATG(9/13)
492 CATCC
HapII 153 CCGG C^CGG
351 CCGG
HinfI 101 GAATC G^A[ACGT]TC
220 GATTC
HpaII 153 CCGG C^CGG
351 CCGG
MspA1I 364 CAGCTG C[AC]G^C[GT]G
438 CCGCTG
MspI 153 CCGG C^CGG
351 CCGG
RsaI 22 GTAC GT^AC
84 GTAC
SfcI 226 CTACAG C^T[GA][CT]AG
293 CTGCAG
StuI 147 AGGCCT AGG^CCT
485 AGGCCT
TfiI 101 GAATC G^A[AT]TC
220 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BsaJI BsgI BsiYI BslI BsmFI
Bsp1286I BspMI BssKI BstNI Cac8I
Cfr13I DraII EcoO109I EcoRII Fnu4HI
HaeIII ItaI MboII MnlI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
TseI