(Circular) (Six-base) MAPSORT of: pFN21AE1411.seq Check: 5648 from: 1 to: 5302 pFN21AE1411 5302 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2291 2470 2291 Size: 179 5123 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3112 278 Size: 53 83 186 936 1576 2468 Fragments arranged by size: 2468 1576 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2645 1937 Size: 708 4594 Acc65I G'GTAC_C Cuts at: 2623 2623 Size: 5302 AclI AA'CG_TT Cuts at: 1563 3429 3802 1563 Size: 1866 373 3063 Fragments arranged by size: 3063 1866 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2069 2282 3365 4749 5017 1632 Size: 437 213 1083 1384 268 1917 Fragments arranged by size: 1917 1384 1083 437 268 213 AfeI AGC'GCT Cuts at: 2513 2513 Size: 5302 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5105 AflIII A'CryG_T Cuts at: 1203 2592 4201 5178 1203 Size: 1389 1609 977 1327 Fragments arranged by size: 1609 1389 1327 977 AgeI A'CCGG_T Cuts at: 4133 4133 Size: 5302 AhdI GACnn_n'nnGTC Cuts at: 4031 4031 Size: 5302 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5302 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5302 AlwNI CAG_nnn'CTG Cuts at: 2383 2542 3045 4617 2383 Size: 159 503 1572 3068 Fragments arranged by size: 3068 1572 503 159 ApaI G_GGCC'C Cuts at: 2201 2390 2201 Size: 189 5113 ApaLI G'TGCA_C Cuts at: 3359 4515 3359 Size: 1156 4146 ApoI r'AATT_y Cuts at: 1493 2611 2837 2989 5098 1493 Size: 1118 226 152 2109 1697 Fragments arranged by size: 2109 1697 1118 226 152 AseI AT'TA_AT Cuts at: 160 1032 3856 160 Size: 872 2824 1606 Fragments arranged by size: 2824 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5302 AvaI C'yCGr_G Cuts at: 1870 1943 2627 1870 Size: 73 684 4545 Fragments arranged by size: 4545 684 73 BamHI G'GATC_C Cuts at: 2319 2632 2999 2319 Size: 313 367 4622 Fragments arranged by size: 4622 367 313 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2623 4078 618 Size: 325 217 627 836 1455 1842 Fragments arranged by size: 1842 1455 836 627 325 217 BanII G_rGCy'C Cuts at: 1391 2201 2390 2403 2621 1391 Size: 810 189 13 218 4072 Fragments arranged by size: 4072 810 218 189 13 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4331 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2279 2313 3493 3527 1903 Size: 34 342 34 1180 34 3678 Fragments arranged by size: 3678 1180 342 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3194 4404 3194 Size: 1210 4092 BclI T'GATC_A Cuts at: 1548 1548 Size: 5302 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3913 136 Size: 107 122 71 631 605 337 1904 1525 Fragments arranged by size: 1904 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5297 5297 Size: 5302 BlpI GC'TnA_GC Cuts at: 2719 2719 Size: 5302 Bme1580I G_kGCm'C Cuts at: 1790 2201 2390 3363 4519 1790 Size: 411 189 973 1156 2573 Fragments arranged by size: 2573 1156 973 411 189 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5302 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2325 3033 3991 Size: 416 53 83 399 24 908 708 958 Cuts at: 3991 442 Size: 1753 Fragments arranged by size: 1753 958 908 708 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5302 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2066 3962 1239 Size: 827 1896 2579 Fragments arranged by size: 2579 1896 827 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3427 4292 4590 3427 Size: 865 298 4139 Fragments arranged by size: 4139 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3965 882 Size: 617 2466 2219 Fragments arranged by size: 2466 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3093 493 Size: 711 1889 2702 Fragments arranged by size: 2702 1889 711 BsaBI GATnn'nnATC Cuts at: 5296 5296 Size: 5302 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3109 3491 4126 275 Size: 53 83 186 936 1576 382 635 1451 Fragments arranged by size: 1576 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2157 3735 4133 4407 4554 4965 2157 Size: 1578 398 274 147 411 2494 Fragments arranged by size: 2494 1578 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2013 2043 1178 Size: 30 165 30 610 30 4437 Fragments arranged by size: 4437 610 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5302 BseYI C'CCAG_C Cuts at: 2050 2383 4505 5001 2050 Size: 333 2122 496 2351 Fragments arranged by size: 2351 2122 496 333 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2076 2492 2528 2573 1163 Size: 573 340 416 36 45 3892 Fragments arranged by size: 3892 573 416 340 45 36 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2752 3513 4541 1540 Size: 275 186 751 761 1028 2301 Fragments arranged by size: 2301 1028 761 751 275 186 BsiHKAI G_wGCw'C Cuts at: 3363 3448 4519 3363 Size: 85 1071 4146 Fragments arranged by size: 4146 1071 85 BsmI GAATG_Cn' Cuts at: 2813 2906 2813 Size: 93 5209 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2201 2390 2403 2621 3363 3448 4519 Size: 399 411 189 13 218 742 85 1071 Cuts at: 4519 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 411 399 218 189 85 13 BspEI T'CCGG_A Cuts at: 2157 4965 2157 Size: 2808 2494 BspHI T'CATG_A Cuts at: 3191 4921 3191 Size: 1730 3572 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2291 2301 2470 2657 844 Size: 1447 10 169 187 3489 Fragments arranged by size: 3489 1447 187 169 10 BsrBI CCG'CTC Cuts at: 1394 1823 2034 3189 1394 Size: 429 211 1155 3507 Fragments arranged by size: 3507 1155 429 211 BsrDI GCAATG_nn' Cuts at: 65 1248 3797 3971 65 Size: 1183 2549 174 1396 Fragments arranged by size: 2549 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2355 3946 4133 1700 Size: 110 545 1591 187 2869 Fragments arranged by size: 2869 1591 545 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5302 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5302 BssSI C'ACGA_G Cuts at: 3362 4374 3362 Size: 1012 4290 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5302 BstBI TT'CG_AA Cuts at: 4106 4106 Size: 5302 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5302 BstYI r'GATC_y Cuts at: 1972 2319 2632 2999 3384 3401 4853 4939 4951 Size: 347 313 367 385 17 1452 86 12 Cuts at: 4951 5297 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 347 346 313 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2403 513 Size: 552 939 399 3412 Fragments arranged by size: 3412 939 552 399 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4563 Fragments arranged by size: 4563 683 56 BtsI GCAGTG_nn' Cuts at: 2818 3612 3632 2818 Size: 794 20 4488 Fragments arranged by size: 4488 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5302 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5267 DraI TTT'AAA Cuts at: 2606 2606 Size: 5302 DraIII CAC_nnn'GTG Cuts at: 2470 2470 Size: 5302 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4309 809 Size: 507 2993 1802 Fragments arranged by size: 2993 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2749 3638 5267 Size: 54 1425 50 275 18 919 889 1629 Cuts at: 5267 8 Size: 43 Fragments arranged by size: 1629 1425 919 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2749 1537 Size: 275 937 4090 Fragments arranged by size: 4090 937 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2271 3232 1353 Size: 918 961 3423 Fragments arranged by size: 3423 961 918 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3889 4263 4409 387 Size: 1064 138 2300 374 146 1280 Fragments arranged by size: 2300 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 2069 2282 3365 3962 4749 5017 Size: 393 434 3 213 1083 597 787 268 Cuts at: 5017 1239 Size: 1524 Fragments arranged by size: 1524 1083 787 597 434 393 268 213 3 EcoNI CCTnn'n_nnAGG Cuts at: 2096 2248 2096 Size: 152 5150 EcoO109I rG'GnC_Cy Cuts at: 2197 2198 2387 2197 Size: 1 189 5112 Fragments arranged by size: 5112 189 1 EcoRI G'AATT_C Cuts at: 2611 2989 2611 Size: 378 4924 EcoRV GAT'ATC Cuts at: 2182 2437 2182 Size: 255 5047 FspI TGC'GCA Cuts at: 3808 3808 Size: 5302 HaeII r_GCGC'y Cuts at: 1678 2515 4449 1678 Size: 837 1934 2531 Fragments arranged by size: 2531 1934 837 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3943 3975 4017 4049 1373 Size: 32 135 32 2371 32 42 32 2626 Fragments arranged by size: 2626 2371 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2646 2892 669 Size: 988 281 708 246 3079 Fragments arranged by size: 3079 988 708 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5302 HpaI GTT'AAC Cuts at: 2892 2892 Size: 5302 KpnI G_GTAC'C Cuts at: 2627 2627 Size: 5302 MfeI C'AATT_G Cuts at: 2901 2901 Size: 5302 MluI A'CGCG_T Cuts at: 5178 5178 Size: 5302 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2577 4416 4600 1305 Size: 1272 1839 184 2007 Fragments arranged by size: 2007 1839 1272 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3480 Fragments arranged by size: 3480 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3260 3619 3778 518 Size: 648 2094 359 159 2042 Fragments arranged by size: 2094 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2371 2445 2718 3395 4543 4788 1606 Size: 765 74 273 677 1148 245 2120 Fragments arranged by size: 2120 1148 765 677 273 245 74 NcoI C'CATG_G Cuts at: 513 1065 2004 2403 513 Size: 552 939 399 3412 Fragments arranged by size: 3412 939 552 399 NdeI CA'TA_TG Cuts at: 387 387 Size: 5302 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5302 NotI GC'GGCC_GC Cuts at: 2749 2749 Size: 5302 NspI r_CATG'y Cuts at: 1132 1229 2596 2660 4205 1132 Size: 97 1367 64 1545 2229 Fragments arranged by size: 2229 1545 1367 97 64 PasI CC'CwG_GG Cuts at: 1348 2244 1348 Size: 896 4406 PciI A'CATG_T Cuts at: 2592 4201 2592 Size: 1609 3693 PflMI CCAn_nnn'nTGG Cuts at: 1108 2383 1108 Size: 1275 4027 PmeI GTTT'AAAC Cuts at: 2606 2606 Size: 5302 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3371 3403 4884 4916 3371 Size: 32 1481 32 3757 Fragments arranged by size: 3757 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5302 PsiI TTA'TAA Cuts at: 2872 2872 Size: 5302 PspOMI G'GGCC_C Cuts at: 2197 2386 2197 Size: 189 5113 PstI C_TGCA'G Cuts at: 830 2302 2654 830 Size: 1472 352 3478 Fragments arranged by size: 3478 1472 352 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5302 PvuII CAG'CTG Cuts at: 2371 2371 Size: 5302 SalI G'TCGA_C Cuts at: 1936 2644 1936 Size: 708 4594 SbfI CC_TGCA'GG Cuts at: 2302 2654 2302 Size: 352 4950 ScaI AGT'ACT Cuts at: 2028 3550 5262 2028 Size: 1522 1712 2068 Fragments arranged by size: 2068 1712 1522 SfcI C'TryA_G Cuts at: 826 1045 2231 2298 2650 3785 4466 4657 826 Size: 219 1186 67 352 1135 681 191 1471 Fragments arranged by size: 1471 1186 1135 681 352 219 191 67 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5302 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5302 SmaI CCC'GGG Cuts at: 1872 2629 1872 Size: 757 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3406 4307 4569 820 Size: 197 926 1463 901 262 1553 Fragments arranged by size: 1553 1463 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3093 493 Size: 2600 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5302 SphI G_CATG'C Cuts at: 1132 2660 1132 Size: 1528 3774 SspI AAT'ATT Cuts at: 5 52 3226 5 Size: 47 3174 2081 Fragments arranged by size: 3174 2081 47 StuI AGG'CCT Cuts at: 1517 2154 2492 1517 Size: 637 338 4327 Fragments arranged by size: 4327 637 338 StyI C'CwwG_G Cuts at: 513 1065 2004 2403 2741 513 Size: 552 939 399 338 3074 Fragments arranged by size: 3074 939 552 399 338 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2042 2303 3329 3346 3499 3684 4129 Size: 1255 462 261 1026 17 153 185 445 Cuts at: 4129 325 Size: 1498 Fragments arranged by size: 1498 1255 1026 462 445 261 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2026 3548 5260 Size: 274 80 33 51 1443 49 1522 1712 Cuts at: 5260 96 Size: 138 Fragments arranged by size: 1712 1522 1443 274 138 80 51 49 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5262 5294 5262 Size: 32 5270 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5302 XbaI T'CTAG_A Cuts at: 2638 2638 Size: 5302 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5302 XmaI C'CCGG_G Cuts at: 1870 2627 1870 Size: 757 4545 XmnI GAAnn'nnTTC Cuts at: 3431 3431 Size: 5302 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3110 276 Size: 53 83 186 936 1576 2468 Fragments arranged by size: 2468 1576 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI