################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp718I AspEI AvrII BamHI BbeI BbrPI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII ClaI DraI DrdI Eam1105I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI SacII SalI SbfI ScaI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tru9I Tsp509I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 610 GACGTC GACGT^C AccI 937 GTCTAC GT^[AC][GT]AC AfaI 1140 GTAC GT^AC AflIII 239 ACGTGT A^C[GA][CT]GT AocI 510 CCTGAGG CC^T[ACGT]AGG Asp700I 789 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 709 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 351 TGCGCA TGC^GCA BalI 806 TGGCCA TGG^CCA BbsI 307 GTCTTC GAAGAC(2/6) BbvCI 160 CCTCAGC CCTCAGC(-5/-2) BciVI 522 GTATCC GTATCC(6/5) BfaI 568 CTAG C^TAG BpuAI 307 GTCTTC GAAGAC(2/6) BsgI 1053 CTGCAC GTGCAG(16/14) BsiEI 284 CGGCCG CG[GA][CT]^CG BsmI 789 GAATGC GAATGC(1/-1) BstYI 1359 AGATCC [GA]^GATC[CT] Bsu36I 510 CCTGAGG CC^T[ACGT]AGG CpoI 713 CGGTCCG CG^G[AT]CCG DraIII 529 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1370 CCGTGG C^C[GA][CT]GG EagI 284 CGGCCG C^GGCCG EarI 772 CTCTTC CTCTTC(1/4) EclXI 284 CGGCCG C^GGCCG Eco52I 284 CGGCCG C^GGCCG Eco81I 510 CCTGAGG CC^T[ACGT]AGG EcoNI 506 CCTACCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 351 TGCGCA TGC^GCA Ksp632I 772 CTCTTC CTCTTC(1/4) MaeI 568 CTAG C^TAG MflI 1359 AGATCC [GA]^GATC[CT] MluNI 806 TGGCCA TGG^CCA MscI 806 TGGCCA TGG^CCA MslI 988 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PflFI 709 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 990 CAGCTG CAG^CTG RsaI 1140 GTAC GT^AC RsrII 713 CGGTCCG CG^G[AT]CCG SacI 1126 GAGCTC GAGCT^C SapI 771 GCTCTTC GCTCTTC(1/4) SexAI 530 ACCTGGT A^CC[AT]GGT SfcI 694 CTACAG C^T[GA][CT]AG SfiI 66 GGCCCGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC TfiI 1272 GATTC G^A[AT]TC Tth111I 709 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 1359 AGATCC [GA]^GATC[CT] XmnI 789 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcyI 610 GACGTC G[GA]^CG[CT]C 1027 GACGCC AlwI 1360 GATCC GGATC(4/5) 905 GGATC BanI 730 GGCACC G^G[CT][GA]CC 1015 GGCACC BglI 67 GCCCGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1173 GCCCGACTGGC BpmI 55 CTCCAG CTGGAG(16/14) 1390 CTGGAG BsaHI 610 GACGTC G[GA]^CG[CT]C 1027 GACGCC BspMI 733 ACCTGC ACCTGC(4/8) 1109 ACCTGC BsrBI 8 CCGCTC CCGCTC(-3/-3) 289 GAGCGG BsrFI 977 ACCGGC [GA]^CCGG[CT] 1018 ACCGGC BssHII 428 GCGCGC G^CGCGC 557 GCGCGC Cfr10I 977 ACCGGC [GA]^CCGG[CT] 1018 ACCGGC Hin1I 610 GACGTC G[GA]^CG[CT]C 1027 GACGCC MaeII 239 ACGT A^CGT 611 ACGT NlaIII 777 CATG CATG^ 810 CATG PpuMI 758 AGGACCT [GA]G^G[AT]CC[CT] 843 GGGTCCC SfaNI 457 GATGC GCATC(5/9) 495 GATGC StuI 579 AGGCCT AGG^CCT 1049 AGGCCT TaqI 134 TCGA T^CGA 1334 TCGA TthHB8I 134 TCGA T^CGA 1334 TCGA XcmI 830 CCACTGACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1238 CCAACGGGAACCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccII AciI AluI AlwNI Aor51HI ApaI AspHI AvaI AvaII BanII BbvI BcnI BmyI BsaJI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI Eco47III EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIV PflMI PleI Sau3AI Sau96I ScrFI TseI Tsp45I TspRI Van91I