(Circular) (Six-base) MAPSORT of: pFN21AE5611.seq Check: 3465 from: 1 to: 6181 pFN21AE5611 6181 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2747 2747 Size: 6181 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2619 3991 278 Size: 53 83 186 936 1083 1372 2468 Fragments arranged by size: 2468 1372 1083 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2943 3524 1937 Size: 1006 581 4594 Fragments arranged by size: 4594 1006 581 Acc65I G'GTAC_C Cuts at: 3502 3502 Size: 6181 AclI AA'CG_TT Cuts at: 1563 4308 4681 1563 Size: 2745 373 3063 Fragments arranged by size: 3063 2745 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4244 5628 5896 1632 Size: 2612 1384 268 1917 Fragments arranged by size: 2612 1917 1384 268 AfeI AGC'GCT Cuts at: 2105 2144 2171 2105 Size: 39 27 6115 Fragments arranged by size: 6115 39 27 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5984 AflIII A'CryG_T Cuts at: 1203 2244 5080 6057 1203 Size: 1041 2836 977 1327 Fragments arranged by size: 2836 1327 1041 977 AgeI A'CCGG_T Cuts at: 5012 5012 Size: 6181 AhdI GACnn_n'nnGTC Cuts at: 4910 4910 Size: 6181 AleI CACnn'nnGTG Cuts at: 1166 2997 1166 Size: 1831 4350 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6181 AlwNI CAG_nnn'CTG Cuts at: 2122 2193 2407 2749 3042 3201 3924 5496 2122 Size: 71 214 342 293 159 723 1572 2807 Fragments arranged by size: 2807 1572 723 342 293 214 159 71 ApaI G_GGCC'C Cuts at: 2026 2074 2083 2214 2458 3468 2026 Size: 48 9 131 244 1010 4739 Fragments arranged by size: 4739 1010 244 131 48 9 ApaLI G'TGCA_C Cuts at: 4238 5394 4238 Size: 1156 5025 ApoI r'AATT_y Cuts at: 1493 3490 3716 3868 5977 1493 Size: 1997 226 152 2109 1697 Fragments arranged by size: 2109 1997 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4735 160 Size: 872 3703 1606 Fragments arranged by size: 3703 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 AvaI C'yCGr_G Cuts at: 1870 1943 2045 2266 2450 2953 3506 1870 Size: 73 102 221 184 503 553 4545 Fragments arranged by size: 4545 553 503 221 184 102 73 BamHI G'GATC_C Cuts at: 3511 3878 3511 Size: 367 5814 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2735 3020 3502 4957 618 Size: 325 217 627 948 285 482 1455 1842 Fragments arranged by size: 1842 1455 948 627 482 325 285 217 BanII G_rGCy'C Cuts at: 1391 2012 2026 2074 2083 2214 2305 2458 2960 Size: 621 14 48 9 131 91 153 502 Cuts at: 2960 3135 3468 3500 1391 Size: 175 333 32 4072 Fragments arranged by size: 4072 621 502 333 175 153 131 91 48 32 14 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2305 928 Size: 971 406 4804 Fragments arranged by size: 4804 971 406 BbvCI CC'TCA_GC Cuts at: 2166 2166 Size: 6181 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4372 4406 1903 Size: 34 2435 34 3678 Fragments arranged by size: 3678 2435 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2538 4073 5283 2538 Size: 1535 1210 3436 Fragments arranged by size: 3436 1535 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6181 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2078 3184 4792 Size: 107 122 71 631 605 406 1106 1608 Cuts at: 4792 136 Size: 1525 Fragments arranged by size: 1608 1525 1106 631 605 406 122 107 71 BglII A'GATC_T Cuts at: 6176 6176 Size: 6181 BlpI GC'TnA_GC Cuts at: 3598 3598 Size: 6181 Bme1580I G_kGCm'C Cuts at: 1790 2026 2074 2083 2214 2379 2458 3468 4242 Size: 236 48 9 131 165 79 1010 774 Cuts at: 4242 5398 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1010 774 236 165 131 79 48 9 BmgBI CAC'GTC Cuts at: 1318 2245 1318 Size: 927 5254 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2238 3912 4870 Size: 416 53 83 399 24 821 1674 958 Cuts at: 4870 442 Size: 1753 Fragments arranged by size: 1753 1674 958 821 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6181 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2045 3416 4841 1239 Size: 806 1371 1425 2579 Fragments arranged by size: 2579 1425 1371 806 Bpu10I CC'TnA_GC Cuts at: 2166 2166 Size: 6181 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4306 5171 5469 4306 Size: 865 298 5018 Fragments arranged by size: 5018 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4844 882 Size: 617 3345 2219 Fragments arranged by size: 3345 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3972 493 Size: 711 2768 2702 Fragments arranged by size: 2768 2702 711 BsaBI GATnn'nnATC Cuts at: 6175 6175 Size: 6181 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2616 3033 3988 4370 Size: 53 83 186 936 1083 417 955 382 Cuts at: 4370 5005 275 Size: 635 1451 Fragments arranged by size: 1451 1083 955 936 635 417 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4614 5012 5286 5433 5844 4614 Size: 398 274 147 411 4951 Fragments arranged by size: 4951 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5956 Fragments arranged by size: 5956 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6181 BseYI C'CCAG_C Cuts at: 2724 2772 5384 5880 2724 Size: 48 2612 496 3025 Fragments arranged by size: 3025 2612 496 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3043 1163 Size: 573 1307 4301 Fragments arranged by size: 4301 1307 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2292 3631 4392 5420 1540 Size: 275 186 291 1339 761 1028 2301 Fragments arranged by size: 2301 1339 1028 761 291 275 186 BsiHKAI G_wGCw'C Cuts at: 2325 2929 2967 3135 4242 4327 5398 2325 Size: 604 38 168 1107 85 1071 3108 Fragments arranged by size: 3108 1107 1071 604 168 85 38 BsmI GAATG_Cn' Cuts at: 2800 3692 3785 2800 Size: 892 93 5196 Fragments arranged by size: 5196 892 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2026 2074 2083 2214 2305 2325 Size: 399 222 14 48 9 131 91 20 Cuts at: 2325 2379 2458 2929 2960 2967 3135 3468 3500 Size: 54 79 471 31 7 168 333 32 Cuts at: 3500 4242 4327 5398 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 471 399 333 222 168 131 91 85 79 54 48 32 31 20 14 9 7 BspEI T'CCGG_A Cuts at: 5844 5844 Size: 6181 BspHI T'CATG_A Cuts at: 4070 5800 4070 Size: 1730 4451 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2747 3123 3536 844 Size: 1903 376 413 3489 Fragments arranged by size: 3489 1903 413 376 BsrBI CCG'CTC Cuts at: 1394 1823 2015 2296 4068 1394 Size: 429 192 281 1772 3507 Fragments arranged by size: 3507 1772 429 281 192 BsrDI GCAATG_nn' Cuts at: 65 1248 4676 4850 65 Size: 1183 3428 174 1396 Fragments arranged by size: 3428 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2982 3023 4825 5012 1700 Size: 110 1172 41 1802 187 2869 Fragments arranged by size: 2869 1802 1172 187 110 41 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6181 BssHII G'CGCG_C Cuts at: 1925 2433 2562 1925 Size: 508 129 5544 Fragments arranged by size: 5544 508 129 BssSI C'ACGA_G Cuts at: 4241 5253 4241 Size: 1012 5169 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6181 BstBI TT'CG_AA Cuts at: 4985 4985 Size: 6181 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6181 BstYI r'GATC_y Cuts at: 1972 3364 3511 3878 4263 4280 5732 5818 5830 Size: 1392 147 367 385 17 1452 86 12 Cuts at: 5830 6176 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1392 385 367 346 147 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2516 2516 Size: 6181 BtgI C'CryG_G Cuts at: 513 1065 2004 3375 513 Size: 552 939 1371 3319 Fragments arranged by size: 3319 1371 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2458 3289 487 Size: 683 56 1232 831 3379 Fragments arranged by size: 3379 1232 831 683 56 BtsI GCAGTG_nn' Cuts at: 2965 3697 4491 4511 2965 Size: 732 794 20 4635 Fragments arranged by size: 4635 794 732 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6181 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6146 DraI TTT'AAA Cuts at: 3485 3485 Size: 6181 DraIII CAC_nnn'GTG Cuts at: 2539 2539 Size: 6181 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5188 809 Size: 507 3872 1802 Fragments arranged by size: 3872 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2289 2409 2556 Size: 54 1425 50 275 18 459 120 147 Cuts at: 2556 2811 3628 4517 6146 8 Size: 255 817 889 1629 43 Fragments arranged by size: 1629 1425 889 817 459 275 255 147 120 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2289 3628 1537 Size: 275 477 1339 4090 Fragments arranged by size: 4090 1339 477 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2783 4111 1353 Size: 1430 1328 3423 Fragments arranged by size: 3423 1430 1328 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2639 3125 4768 5142 5288 387 Size: 1064 138 1050 486 1643 374 146 1280 Fragments arranged by size: 1643 1280 1064 1050 486 374 146 138 EcoICRI GAG'CTC Cuts at: 3133 3133 Size: 6181 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 3416 4244 4841 5628 5896 1239 Size: 393 413 1371 828 597 787 268 1524 Fragments arranged by size: 1524 1371 828 787 597 413 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2515 2515 Size: 6181 EcoO109I rG'GnC_Cy Cuts at: 2023 2070 2079 2080 2210 2404 2454 2764 2849 Size: 47 9 1 130 194 50 310 85 Cuts at: 2849 3039 3464 2023 Size: 190 425 4740 Fragments arranged by size: 4740 425 310 194 190 130 85 50 47 9 1 EcoRI G'AATT_C Cuts at: 3490 3868 3490 Size: 378 5803 FspI TGC'GCA Cuts at: 2358 4687 2358 Size: 2329 3852 HaeII r_GCGC'y Cuts at: 1678 2045 2107 2146 2173 2552 5328 1678 Size: 367 62 39 27 379 2776 2531 Fragments arranged by size: 2776 2531 379 367 62 39 27 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2149 2181 2433 2465 2512 Size: 32 135 32 577 32 252 32 47 Cuts at: 2512 2544 3462 3494 4822 4854 4896 4928 1373 Size: 32 918 32 1328 32 42 32 2626 Fragments arranged by size: 2626 1328 918 577 252 135 47 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3525 3771 669 Size: 988 281 1587 246 3079 Fragments arranged by size: 3079 1587 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6181 HpaI GTT'AAC Cuts at: 3771 3771 Size: 6181 KpnI G_GTAC'C Cuts at: 3506 3506 Size: 6181 MfeI C'AATT_G Cuts at: 3780 3780 Size: 6181 MluI A'CGCG_T Cuts at: 6057 6057 Size: 6181 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2163 2622 2701 3267 5295 5479 1305 Size: 858 459 79 566 2028 184 2007 Fragments arranged by size: 2028 2007 858 566 459 184 79 MscI TGG'CCA Cuts at: 10 64 1489 1832 2813 10 Size: 54 1425 343 981 3378 Fragments arranged by size: 3378 1425 981 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2997 4139 4498 4657 518 Size: 648 1831 1142 359 159 2042 Fragments arranged by size: 2042 1831 1142 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2724 2749 2997 3142 3597 4274 5422 5667 Size: 1118 25 248 145 455 677 1148 245 Cuts at: 5667 1606 Size: 2120 Fragments arranged by size: 2120 1148 1118 677 455 248 245 145 25 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4690 Fragments arranged by size: 4690 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6181 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6181 NotI GC'GGCC_GC Cuts at: 3628 3628 Size: 6181 NspI r_CATG'y Cuts at: 1132 1229 3539 5084 1132 Size: 97 2310 1545 2229 Fragments arranged by size: 2310 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2019 2656 3261 1348 Size: 671 637 605 4268 Fragments arranged by size: 4268 671 637 605 PciI A'CATG_T Cuts at: 5080 5080 Size: 6181 PflMI CCAn_nnn'nTGG Cuts at: 1108 2539 3042 3201 1108 Size: 1431 503 159 4088 Fragments arranged by size: 4088 1431 503 159 PfoI T'CCnGG_A Cuts at: 2097 2610 2097 Size: 513 5668 PmeI GTTT'AAAC Cuts at: 3485 3485 Size: 6181 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4250 4282 5763 5795 4250 Size: 32 1481 32 4636 Fragments arranged by size: 4636 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2764 2849 2764 Size: 85 6096 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6181 PsiI TTA'TAA Cuts at: 3751 3751 Size: 6181 PspOMI G'GGCC_C Cuts at: 2022 2070 2079 2210 2454 3464 2022 Size: 48 9 131 244 1010 4739 Fragments arranged by size: 4739 1010 244 131 48 9 PstI C_TGCA'G Cuts at: 830 3533 830 Size: 2703 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6181 PvuII CAG'CTG Cuts at: 2997 2997 Size: 6181 RsrII CG'GwC_CG Cuts at: 2719 2719 Size: 6181 SacI G_AGCT'C Cuts at: 3135 3135 Size: 6181 SalI G'TCGA_C Cuts at: 1936 3523 1936 Size: 1587 4594 SanDI GG'GwC_CC Cuts at: 2849 2849 Size: 6181 SapI GCTCTTCn'nnn_ Cuts at: 2783 2783 Size: 6181 SbfI CC_TGCA'GG Cuts at: 3533 3533 Size: 6181 ScaI AGT'ACT Cuts at: 4429 6141 4429 Size: 1712 4469 SexAI A'CCwGG_T Cuts at: 2535 2535 Size: 6181 SfcI C'TryA_G Cuts at: 826 1045 2699 3529 4664 5345 5536 826 Size: 219 1654 830 1135 681 191 1471 Fragments arranged by size: 1654 1471 1135 830 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2078 2078 Size: 6181 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6181 SmaI CCC'GGG Cuts at: 1872 3508 1872 Size: 1636 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4285 5186 5448 820 Size: 197 926 2342 901 262 1553 Fragments arranged by size: 2342 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3972 493 Size: 3479 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6181 SphI G_CATG'C Cuts at: 1132 3539 1132 Size: 2407 3774 SspI AAT'ATT Cuts at: 5 52 4105 5 Size: 47 4053 2081 Fragments arranged by size: 4053 2081 47 StuI AGG'CCT Cuts at: 1517 2586 3056 1517 Size: 1069 470 4642 Fragments arranged by size: 4642 1069 470 StyI C'CwwG_G Cuts at: 513 1065 2004 3620 513 Size: 552 939 1616 3074 Fragments arranged by size: 3074 1616 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2669 3113 3372 4208 4225 4378 4563 Size: 1255 1089 444 259 836 17 153 185 Cuts at: 4563 5008 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1089 836 445 444 259 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4427 6139 96 Size: 274 80 33 51 1443 2450 1712 138 Fragments arranged by size: 2450 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2491 2523 3277 3309 6141 6173 2491 Size: 32 754 32 2832 32 2499 Fragments arranged by size: 2832 2499 754 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2717 1316 Size: 1401 4780 XbaI T'CTAG_A Cuts at: 3517 3517 Size: 6181 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2842 3250 2842 Size: 408 5773 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6181 XmaI C'CCGG_G Cuts at: 1870 3506 1870 Size: 1636 4545 XmnI GAAnn'nnTTC Cuts at: 2798 4310 2798 Size: 1512 4669 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2617 3989 276 Size: 53 83 186 936 1083 1372 2468 Fragments arranged by size: 2468 1372 1083 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI SacII SfoI SrfI SwaI