################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI Alw44I AocI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BbeI BfrI BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI EagI EclXI Eco52I Eco81I EcoRV FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PflMI PinAI PmeI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 1651 CGCG CG^CG AflIII 1542 ACACGT A^C[GA][CT]GT AhdI 1388 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 122 AGCGCT AGC^GCT ApaI 1714 GGGCCC GGGCC^C AspEI 1388 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 1745 CTCGAG C^[CT]CG[GA]G AvaII 1387 GGACC G^G[AT]CC BalI 1656 TGGCCA TGG^CCA BamHI 33 GGATCC G^GATCC BanI 1447 GGTGCC G^G[CT][GA]CC BbrPI 1543 CACGTG CAC^GTG BcgI 661 GCATTTAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaAI 1543 CACGTG [CT]AC^GT[GA] BsaWI 419 ACCGGA [AT]^CCGG[AT] BseRI 595 GAGGAG GAGGAG(10/8) BsmI 1058 GAATGC GAATGC(1/-1) BsoBI 1745 CTCGAG C^[CT]CG[GA]G Bsp1407I 1103 TGTACA T^GTACA BspHI 39 TCATGA T^CATGA BsrGI 1103 TGTACA T^GTACA BstF5I 204 GGATG GGATG(2/0) BstUI 1651 CGCG CG^CG BstXI 1331 CCAACCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 1554 TTTAAA TTT^AAA EaeI 1656 TGGCCA [CT]^GGCC[GA] Eam1105I 1388 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1256 GAAGAG CTCTTC(1/4) Eco47III 122 AGCGCT AGC^GCT EcoNI 937 CCTGGGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 445 GAATTC G^AATTC EcoT22I 659 ATGCAT ATGCA^T FokI 204 GGATG GGATG(9/13) Ksp632I 1256 GAAGAG CTCTTC(1/4) MfeI 472 CAATTG C^AATTG MluNI 1656 TGGCCA TGG^CCA MscI 1656 TGGCCA TGG^CCA MunI 472 CAATTG C^AATTG MvnI 1651 CGCG CG^CG NcoI 1577 CCATGG C^CATGG NsiI 659 ATGCAT ATGCA^T PaeR7I 1745 CTCGAG C^TCGAG PmaCI 1543 CACGTG CAC^GTG PmlI 1543 CACGTG CAC^GTG PpuMI 1386 GGGACCC [GA]G^G[AT]CC[CT] PshAI 926 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 39 TCATGA T^CATGA ScaI 73 AGTACT AGT^ACT SexAI 635 ACCAGGT A^CC[AT]GGT SmlI 1745 CTCGAG C^T[CT][GA]AG SspBI 1103 TGTACA T^GTACA XhoI 1745 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AfaI 74 GTAC GT^AC 1104 GTAC AspHI 254 GTGCTC G[AT]GC[AT]^C 1411 GTGCTC BanII 996 GGGCTC G[GA]GC[CT]^C 1714 GGGCCC BbsI 109 GAAGAC GAAGAC(2/6) 1050 GAAGAC BbvCI 1718 CCTCAGC CCTCAGC(-5/-2) 886 GCTGAGG BciVI 1912 GGATAC GTATCC(6/5) 1032 GTATCC BclI 192 TGATCA T^GATCA 1899 TGATCA BcnI 1416 CCGGG CC^[GC]GG 1704 CCGGG BglI 877 GCCCCATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 951 GCCTATCTGGC BglII 568 AGATCT A^GATCT 1041 AGATCT BpuAI 109 GAAGAC GAAGAC(2/6) 1050 GAAGAC BsgI 13 GTGCAG GTGCAG(16/14) 169 GTGCAG BsiHKAI 254 GTGCTC G[AT]GC[AT]^C 1411 GTGCTC BspMI 1582 GCAGGT ACCTGC(4/8) 1638 GCAGGT BsrDI 1510 CATTGC GCAATG(2/0) 1872 CATTGC BstEII 558 GGTTACC G^GT[ACGT]ACC 1605 GGTGACC BstPI 558 GGTTACC G^GT[ACGT]ACC 1605 GGTGACC DsaI 1382 CCACGG C^C[GA][CT]GG 1577 CCATGG EcoO65I 558 GGTTACC G^GT[ACGT]ACC 1605 GGTGACC FauI 1649 CCCGC CCCGC(4/6) 1840 CCCGC FbaI 192 TGATCA T^GATCA 1899 TGATCA MaeII 1544 ACGT A^CGT 1933 ACGT MslI 1023 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1903 CACATCGGTG NciI 1416 CCGGG CC^[GC]GG 1704 CCGGG NspI 480 ACATGC [GA]CATG^[CT] 575 ACATGC RsaI 74 GTAC GT^AC 1104 GTAC StuI 949 AGGCCT AGG^CCT 1830 AGGCCT XcmI 266 CCATGTTCATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1068 CCAGCGTGGCTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AcsI AluI AlwI AlwNI ApoI BbvI BfaI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII