(Circular) (Six-base) MAPSORT of: pFN21AB3491.seq Check: 7334 from: 1 to: 6739 pFN21AB3491 6739 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3578 3634 3578 Size: 56 6683 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4549 278 Size: 53 83 186 936 3013 2468 Fragments arranged by size: 3013 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4082 1937 Size: 2145 4594 Acc65I G'GTAC_C Cuts at: 4060 4060 Size: 6739 AclI AA'CG_TT Cuts at: 1563 4866 5239 1563 Size: 3303 373 3063 Fragments arranged by size: 3303 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2829 2909 4802 6186 6454 1632 Size: 1197 80 1893 1384 268 1917 Fragments arranged by size: 1917 1893 1384 1197 268 80 AfeI AGC'GCT Cuts at: 2129 2129 Size: 6739 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6542 AflIII A'CryG_T Cuts at: 1203 3547 5638 6615 1203 Size: 2344 2091 977 1327 Fragments arranged by size: 2344 2091 1327 977 AgeI A'CCGG_T Cuts at: 5570 5570 Size: 6739 AhdI GACnn_n'nnGTC Cuts at: 3398 5468 3398 Size: 2070 4669 AleI CACnn'nnGTG Cuts at: 1166 3032 3912 1166 Size: 1866 880 3993 Fragments arranged by size: 3993 1866 880 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3666 1184 Size: 2482 4257 AlwNI CAG_nnn'CTG Cuts at: 2473 2621 3011 4482 6054 2473 Size: 148 390 1471 1572 3158 Fragments arranged by size: 3158 1572 1471 390 148 ApaI G_GGCC'C Cuts at: 3723 3723 Size: 6739 ApaLI G'TGCA_C Cuts at: 4796 5952 4796 Size: 1156 5583 ApoI r'AATT_y Cuts at: 1493 2343 2396 2450 2759 3251 3432 3959 3980 Size: 850 53 54 309 492 181 527 21 Cuts at: 3980 4048 4274 4426 6535 1493 Size: 68 226 152 2109 1697 Fragments arranged by size: 2109 1697 850 527 492 309 226 181 152 68 54 53 21 AseI AT'TA_AT Cuts at: 160 1032 5293 160 Size: 872 4261 1606 Fragments arranged by size: 4261 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6739 AvaI C'yCGr_G Cuts at: 1870 1943 3750 4064 1870 Size: 73 1807 314 4545 Fragments arranged by size: 4545 1807 314 73 BamHI G'GATC_C Cuts at: 2038 4069 4436 2038 Size: 2031 367 4341 Fragments arranged by size: 4341 2031 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3452 4060 5515 618 Size: 325 217 627 1665 608 1455 1842 Fragments arranged by size: 1842 1665 1455 627 608 325 217 BanII G_rGCy'C Cuts at: 1391 3005 3723 4058 1391 Size: 1614 718 335 4072 Fragments arranged by size: 4072 1614 718 335 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2121 3062 928 Size: 971 222 941 4605 Fragments arranged by size: 4605 971 941 222 BbvCI CC'TCA_GC Cuts at: 2892 3724 2892 Size: 832 5907 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2655 2689 4930 4964 1903 Size: 34 718 34 2241 34 3678 Fragments arranged by size: 3678 2241 718 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3048 3911 4631 5841 3048 Size: 863 720 1210 3946 Fragments arranged by size: 3946 1210 863 720 BclI T'GATC_A Cuts at: 1548 2197 3904 1548 Size: 649 1707 4383 Fragments arranged by size: 4383 1707 649 BfrBI ATG'CAT Cuts at: 2666 2666 Size: 6739 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2888 2962 5350 Size: 107 122 71 631 605 1216 74 2388 Cuts at: 5350 136 Size: 1525 Fragments arranged by size: 2388 1525 1216 631 605 122 107 74 71 BglII A'GATC_T Cuts at: 2573 3046 6734 2573 Size: 473 3688 2578 Fragments arranged by size: 3688 2578 473 BlpI GC'TnA_GC Cuts at: 4156 4156 Size: 6739 Bme1580I G_kGCm'C Cuts at: 1790 3723 4800 5956 1790 Size: 1933 1077 1156 2573 Fragments arranged by size: 2573 1933 1156 1077 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6739 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2395 3766 4470 Size: 416 53 83 399 24 978 1371 704 Cuts at: 4470 5428 442 Size: 958 1753 Fragments arranged by size: 1753 1371 978 958 704 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6739 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2247 2450 2643 2951 3033 3189 3321 3333 Size: 1008 203 193 308 82 156 132 12 Cuts at: 3333 3396 3597 3791 5399 1239 Size: 63 201 194 1608 2579 Fragments arranged by size: 2579 1608 1008 308 203 201 194 193 156 132 82 63 12 Bpu10I CC'TnA_GC Cuts at: 2892 3724 2892 Size: 832 5907 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4864 5729 6027 4864 Size: 865 298 5576 Fragments arranged by size: 5576 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5402 882 Size: 617 3903 2219 Fragments arranged by size: 3903 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3550 4530 493 Size: 711 2346 980 2702 Fragments arranged by size: 2702 2346 980 711 BsaBI GATnn'nnATC Cuts at: 6733 6733 Size: 6739 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4546 4928 5563 275 Size: 53 83 186 936 3013 382 635 1451 Fragments arranged by size: 3013 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2424 5172 5570 5844 5991 6402 2424 Size: 2748 398 274 147 411 2761 Fragments arranged by size: 2761 2748 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3092 3122 1178 Size: 30 165 30 1689 30 4795 Fragments arranged by size: 4795 1689 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2615 1186 Size: 1429 5310 BseYI C'CCAG_C Cuts at: 3595 5942 6438 3595 Size: 2347 496 3896 Fragments arranged by size: 3896 2347 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2039 2195 1163 Size: 573 303 156 5707 Fragments arranged by size: 5707 573 303 156 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4189 4950 5978 1540 Size: 275 186 2188 761 1028 2301 Fragments arranged by size: 2301 2188 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2263 3420 4800 4885 5956 2263 Size: 1157 1380 85 1071 3046 Fragments arranged by size: 3046 1380 1157 1071 85 BsmI GAATG_Cn' Cuts at: 3069 4250 4343 3069 Size: 1181 93 5465 Fragments arranged by size: 5465 1181 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2263 3005 3420 3723 4058 4800 4885 Size: 399 473 742 415 303 335 742 85 Cuts at: 4885 5956 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 742 473 415 399 335 303 85 BspEI T'CCGG_A Cuts at: 6402 6402 Size: 6739 BspHI T'CATG_A Cuts at: 2044 4628 6358 2044 Size: 2584 1730 2425 Fragments arranged by size: 2584 2425 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3578 3634 4094 844 Size: 2734 56 460 3489 Fragments arranged by size: 3489 2734 460 56 BsrBI CCG'CTC Cuts at: 1394 1823 4626 1394 Size: 429 2803 3507 Fragments arranged by size: 3507 2803 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3514 3876 5234 5408 65 Size: 1183 2266 362 1358 174 1396 Fragments arranged by size: 2266 1396 1358 1183 362 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5383 5570 1700 Size: 110 3573 187 2869 Fragments arranged by size: 3573 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3108 96 Size: 3012 3727 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6739 BssSI C'ACGA_G Cuts at: 4799 5811 4799 Size: 1012 5727 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6739 BstBI TT'CG_AA Cuts at: 5543 5543 Size: 6739 BstEII G'GTnAC_C Cuts at: 1184 2563 3610 1184 Size: 1379 1047 4313 Fragments arranged by size: 4313 1379 1047 BstXI CCAn_nnnn'nTGG Cuts at: 3343 3343 Size: 6739 BstYI r'GATC_y Cuts at: 1972 2038 2573 3046 4069 4436 4821 4838 6290 Size: 66 535 473 1023 367 385 17 1452 Cuts at: 6290 6376 6388 6734 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1023 535 473 385 367 346 86 66 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3387 3582 513 Size: 552 939 1383 195 3670 Fragments arranged by size: 3670 1383 939 552 195 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6000 Fragments arranged by size: 6000 683 56 BtsI GCAGTG_nn' Cuts at: 3027 3039 4029 4255 5049 5069 3027 Size: 12 990 226 794 20 4697 Fragments arranged by size: 4697 990 794 226 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6739 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6704 DraI TTT'AAA Cuts at: 3561 4043 3561 Size: 482 6257 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5746 809 Size: 507 4430 1802 Fragments arranged by size: 4430 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3661 4186 5075 Size: 54 1425 50 275 18 1831 525 889 Cuts at: 5075 6704 8 Size: 1629 43 Fragments arranged by size: 1831 1629 1425 889 525 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4186 1537 Size: 275 2374 4090 Fragments arranged by size: 4090 2374 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3256 4669 1353 Size: 1903 1413 3423 Fragments arranged by size: 3423 1903 1413 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5326 5700 5846 387 Size: 1064 138 3737 374 146 1280 Fragments arranged by size: 3737 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2247 2450 2643 2829 2909 2951 3033 Size: 393 615 203 193 186 80 42 82 Cuts at: 3033 3189 3321 3333 3396 3597 3791 4802 5399 Size: 156 132 12 63 201 194 1011 597 Cuts at: 5399 6186 6454 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1011 787 615 597 393 268 203 201 194 193 186 156 132 82 80 63 42 12 EcoNI CCTnn'n_nnAGG Cuts at: 2946 2946 Size: 6739 EcoO109I rG'GnC_Cy Cuts at: 2898 3392 3719 3720 2898 Size: 494 327 1 5917 Fragments arranged by size: 5917 494 327 1 EcoRI G'AATT_C Cuts at: 2450 4048 4426 2450 Size: 1598 378 4763 Fragments arranged by size: 4763 1598 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3157 3157 Size: 6739 FspI TGC'GCA Cuts at: 5245 5245 Size: 6739 HaeII r_GCGC'y Cuts at: 1678 2131 2854 3408 5886 1678 Size: 453 723 554 2478 2531 Fragments arranged by size: 2531 2478 723 554 453 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2209 2241 2524 2556 2616 Size: 32 135 32 637 32 283 32 60 Cuts at: 2616 2648 3384 3416 3733 3765 3909 3941 5380 Size: 32 736 32 317 32 144 32 1439 Cuts at: 5380 5412 5454 5486 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1439 736 637 317 283 144 135 60 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4083 4329 669 Size: 988 281 2145 246 3079 Fragments arranged by size: 3079 2145 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6739 HpaI GTT'AAC Cuts at: 4329 4329 Size: 6739 KpnI G_GTAC'C Cuts at: 4064 4064 Size: 6739 MfeI C'AATT_G Cuts at: 2477 4338 2477 Size: 1861 4878 MluI A'CGCG_T Cuts at: 6615 6615 Size: 6739 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2428 5853 6037 1305 Size: 1123 3425 184 2007 Fragments arranged by size: 3425 2007 1123 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3663 10 Size: 54 1425 343 1831 3086 Fragments arranged by size: 3086 1831 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3032 3912 4697 5056 5215 518 Size: 648 1866 880 785 359 159 2042 Fragments arranged by size: 2042 1866 880 785 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2621 3575 3599 3811 3848 4155 4832 5980 Size: 1015 954 24 212 37 307 677 1148 Cuts at: 5980 6225 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1015 954 677 307 245 212 37 24 NcoI C'CATG_G Cuts at: 513 1065 2004 3582 513 Size: 552 939 1578 3670 Fragments arranged by size: 3670 1578 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6739 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6739 NotI GC'GGCC_GC Cuts at: 4186 4186 Size: 6739 NsiI A_TGCA'T Cuts at: 2668 2668 Size: 6739 NspI r_CATG'y Cuts at: 1132 1229 2489 2584 4097 5642 1132 Size: 97 1260 95 1513 1545 2229 Fragments arranged by size: 2229 1545 1513 1260 97 95 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6739 PciI A'CATG_T Cuts at: 5638 5638 Size: 6739 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6739 PfoI T'CCnGG_A Cuts at: 2466 3420 3708 2466 Size: 954 288 5497 Fragments arranged by size: 5497 954 288 PmeI GTTT'AAAC Cuts at: 4043 4043 Size: 6739 PmlI CAC'GTG Cuts at: 3550 3550 Size: 6739 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4808 4840 6321 6353 4808 Size: 32 1481 32 5194 Fragments arranged by size: 5194 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3392 3392 Size: 6739 PshAI GACnn'nnGTC Cuts at: 1613 2935 1613 Size: 1322 5417 PsiI TTA'TAA Cuts at: 4309 4309 Size: 6739 PspOMI G'GGCC_C Cuts at: 3719 3719 Size: 6739 PspXI vC'TCGA_Gb Cuts at: 3750 3750 Size: 6739 PstI C_TGCA'G Cuts at: 830 3034 3645 3748 4091 830 Size: 2204 611 103 343 3478 Fragments arranged by size: 3478 2204 611 343 103 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6739 PvuII CAG'CTG Cuts at: 2621 3575 3599 2621 Size: 954 24 5761 Fragments arranged by size: 5761 954 24 SalI G'TCGA_C Cuts at: 1936 4081 1936 Size: 2145 4594 SanDI GG'GwC_CC Cuts at: 3392 3392 Size: 6739 SbfI CC_TGCA'GG Cuts at: 4091 4091 Size: 6739 ScaI AGT'ACT Cuts at: 2080 4987 6699 2080 Size: 2907 1712 2120 Fragments arranged by size: 2907 2120 1712 SexAI A'CCwGG_T Cuts at: 2640 2640 Size: 6739 SfcI C'TryA_G Cuts at: 826 1045 2066 3030 3042 3169 3641 3744 4087 Size: 219 1021 964 12 127 472 103 343 Cuts at: 4087 5222 5903 6094 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1021 964 681 472 343 219 191 127 103 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6739 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6739 SmaI CCC'GGG Cuts at: 1872 4066 1872 Size: 2194 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3750 4843 5744 6006 820 Size: 197 926 1807 1093 901 262 1553 Fragments arranged by size: 1807 1553 1093 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4530 493 Size: 4037 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6739 SphI G_CATG'C Cuts at: 1132 4097 1132 Size: 2965 3774 SspI AAT'ATT Cuts at: 5 52 4663 5 Size: 47 4611 2081 Fragments arranged by size: 4611 2081 47 StuI AGG'CCT Cuts at: 1517 2956 3837 1517 Size: 1439 881 4419 Fragments arranged by size: 4419 1439 881 StyI C'CwwG_G Cuts at: 513 1065 2004 2205 3582 3786 4178 513 Size: 552 939 201 1377 204 392 3074 Fragments arranged by size: 3074 1377 939 552 392 204 201 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4766 4783 4936 5121 5566 325 Size: 1255 3186 17 153 185 445 1498 Fragments arranged by size: 3186 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2078 3108 4985 Size: 274 80 33 51 1443 101 1030 1877 Cuts at: 4985 6697 96 Size: 1712 138 Fragments arranged by size: 1877 1712 1443 1030 274 138 101 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6699 6731 6699 Size: 32 6707 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6739 XbaI T'CTAG_A Cuts at: 4075 4075 Size: 6739 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2278 3080 2278 Size: 802 5937 XhoI C'TCGA_G Cuts at: 1943 3750 1943 Size: 1807 4932 XmaI C'CCGG_G Cuts at: 1870 4064 1870 Size: 2194 4545 XmnI GAAnn'nnTTC Cuts at: 4868 4868 Size: 6739 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4547 276 Size: 53 83 186 936 3013 2468 Fragments arranged by size: 3013 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI