Restriction map for
pFN21AB3491
(Linear) (Six-base) MAPSORT of: KIBB3491.seq Check: 1828 from: 1 to: 2034
KIBB3491.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1573 1629 2034
Size: 1573 56 405
Fragments arranged by size:
1573 405 56
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 824 904 2034
Size: 824 80 1130
Fragments arranged by size:
1130 824 80
AfeI AGC'GCT
Cuts at: 0 124 2034
Size: 124 1910
AflIII A'CryG_T
Cuts at: 0 1542 2034
Size: 1542 492
AhdI GACnn_n'nnGTC
Cuts at: 0 1393 2034
Size: 1393 641
AleI CACnn'nnGTG
Cuts at: 0 1027 1907 2034
Size: 1027 880 127
Fragments arranged by size:
1027 880 127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1661 2034
Size: 1661 373
AlwNI CAG_nnn'CTG
Cuts at: 0 468 616 1006 2034
Size: 468 148 390 1028
Fragments arranged by size:
1028 468 390 148
ApaI G_GGCC'C
Cuts at: 0 1718 2034
Size: 1718 316
ApoI r'AATT_y
Cuts at: 0 338 391 445 754 1246 1427 1954 1975
Size: 338 53 54 309 492 181 527 21
Cuts at: 1975 2034
Size: 59
Fragments arranged by size:
527 492 338 309 181 59 54 53
21
AvaI C'yCGr_G
Cuts at: 0 1745 2034
Size: 1745 289
BamHI G'GATC_C
Cuts at: 0 33 2034
Size: 33 2001
BanI G'GyrC_C
Cuts at: 0 1447 2034
Size: 1447 587
BanII G_rGCy'C
Cuts at: 0 1000 1718 2034
Size: 1000 718 316
Fragments arranged by size:
1000 718 316
BbsI GAAGACnn'nnnn_
Cuts at: 0 116 1057 2034
Size: 116 941 977
Fragments arranged by size:
977 941 116
BbvCI CC'TCA_GC
Cuts at: 0 887 1719 2034
Size: 887 832 315
Fragments arranged by size:
887 832 315
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 650 684 2034
Size: 650 34 1350
Fragments arranged by size:
1350 650 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1043 1906 2034
Size: 1043 863 128
Fragments arranged by size:
1043 863 128
BclI T'GATC_A
Cuts at: 0 192 1899 2034
Size: 192 1707 135
Fragments arranged by size:
1707 192 135
BfrBI ATG'CAT
Cuts at: 0 661 2034
Size: 661 1373
BglI GCCn_nnn'nGGC
Cuts at: 0 883 957 2034
Size: 883 74 1077
Fragments arranged by size:
1077 883 74
BglII A'GATC_T
Cuts at: 0 568 1041 2034
Size: 568 473 993
Fragments arranged by size:
993 568 473
Bme1580I G_kGCm'C
Cuts at: 0 1718 2034
Size: 1718 316
BmrI ACTGGGnnnn_n'
Cuts at: 0 390 1761 2034
Size: 390 1371 273
Fragments arranged by size:
1371 390 273
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 445 638 946 1028 1184 1316 1328
Size: 242 203 193 308 82 156 132 12
Cuts at: 1328 1391 1592 1786 2034
Size: 63 201 194 248
Fragments arranged by size:
308 248 242 203 201 194 193 156
132 82 63 12
Bpu10I CC'TnA_GC
Cuts at: 0 887 1719 2034
Size: 887 832 315
Fragments arranged by size:
887 832 315
BsaAI yAC'GTr
Cuts at: 0 1545 2034
Size: 1545 489
BsaWI w'CCGG_w
Cuts at: 0 419 2034
Size: 419 1615
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1087 1117 2034
Size: 1087 30 917
Fragments arranged by size:
1087 917 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 610 2034
Size: 610 1424
BseYI C'CCAG_C
Cuts at: 0 1590 2034
Size: 1590 444
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 190 2034
Size: 34 156 1844
Fragments arranged by size:
1844 156 34
BsiHKAI G_wGCw'C
Cuts at: 0 258 1415 2034
Size: 258 1157 619
Fragments arranged by size:
1157 619 258
BsmI GAATG_Cn'
Cuts at: 0 1064 2034
Size: 1064 970
Bsp1286I G_dGCh'C
Cuts at: 0 258 1000 1415 1718 2034
Size: 258 742 415 303 316
Fragments arranged by size:
742 415 316 303 258
BspHI T'CATG_A
Cuts at: 0 39 2034
Size: 39 1995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1573 1629 2034
Size: 1573 56 405
Fragments arranged by size:
1573 405 56
BsrDI GCAATG_nn'
Cuts at: 0 1509 1871 2034
Size: 1509 362 163
Fragments arranged by size:
1509 362 163
BsrGI T'GTAC_A
Cuts at: 0 1103 2034
Size: 1103 931
BstEII G'GTnAC_C
Cuts at: 0 558 1605 2034
Size: 558 1047 429
Fragments arranged by size:
1047 558 429
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1338 2034
Size: 1338 696
BstYI r'GATC_y
Cuts at: 0 33 568 1041 2034
Size: 33 535 473 993
Fragments arranged by size:
993 535 473 33
BtgI C'CryG_G
Cuts at: 0 1382 1577 2034
Size: 1382 195 457
Fragments arranged by size:
1382 457 195
BtsI GCAGTG_nn'
Cuts at: 0 1022 1034 2024 2034
Size: 1022 12 990 10
Fragments arranged by size:
1022 990 12 10
DraI TTT'AAA
Cuts at: 0 1556 2034
Size: 1556 478
EaeI y'GGCC_r
Cuts at: 0 1656 2034
Size: 1656 378
EarI CTCTTCn'nnn_
Cuts at: 0 1251 2034
Size: 1251 783
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 445 638 824 904 946 1028 1184
Size: 242 203 193 186 80 42 82 156
Cuts at: 1184 1316 1328 1391 1592 1786 2034
Size: 132 12 63 201 194 248
Fragments arranged by size:
248 242 203 201 194 193 186 156
132 82 80 63 42 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 941 2034
Size: 941 1093
EcoO109I rG'GnC_Cy
Cuts at: 0 893 1387 1714 1715 2034
Size: 893 494 327 1 319
Fragments arranged by size:
893 494 327 319 1
EcoRI G'AATT_C
Cuts at: 0 445 2034
Size: 445 1589
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1152 2034
Size: 1152 882
HaeII r_GCGC'y
Cuts at: 0 126 849 1403 2034
Size: 126 723 554 631
Fragments arranged by size:
723 631 554 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 204 236 519 551 611 643 1379 1411
Size: 204 32 283 32 60 32 736 32
Cuts at: 1411 1728 1760 1904 1936 2034
Size: 317 32 144 32 98
Fragments arranged by size:
736 317 283 204 144 98 60 32
32 32 32 32 32
MfeI C'AATT_G
Cuts at: 0 472 2034
Size: 472 1562
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 423 2034
Size: 423 1611
MscI TGG'CCA
Cuts at: 0 1658 2034
Size: 1658 376
MslI CAynn'nnrTG
Cuts at: 0 1027 1907 2034
Size: 1027 880 127
Fragments arranged by size:
1027 880 127
MspA1I CmG'CkG
Cuts at: 0 616 1570 1594 1806 1843 2034
Size: 616 954 24 212 37 191
Fragments arranged by size:
954 616 212 191 37 24
NcoI C'CATG_G
Cuts at: 0 1577 2034
Size: 1577 457
NsiI A_TGCA'T
Cuts at: 0 663 2034
Size: 663 1371
NspI r_CATG'y
Cuts at: 0 484 579 2034
Size: 484 95 1455
Fragments arranged by size:
1455 484 95
PfoI T'CCnGG_A
Cuts at: 0 461 1415 1703 2034
Size: 461 954 288 331
Fragments arranged by size:
954 461 331 288
PmlI CAC'GTG
Cuts at: 0 1545 2034
Size: 1545 489
PpuMI rG'GwC_Cy
Cuts at: 0 1387 2034
Size: 1387 647
PshAI GACnn'nnGTC
Cuts at: 0 930 2034
Size: 930 1104
PspOMI G'GGCC_C
Cuts at: 0 1714 2034
Size: 1714 320
PspXI vC'TCGA_Gb
Cuts at: 0 1745 2034
Size: 1745 289
PstI C_TGCA'G
Cuts at: 0 1029 1640 1743 2034
Size: 1029 611 103 291
Fragments arranged by size:
1029 611 291 103
PvuII CAG'CTG
Cuts at: 0 616 1570 1594 2034
Size: 616 954 24 440
Fragments arranged by size:
954 616 440 24
SanDI GG'GwC_CC
Cuts at: 0 1387 2034
Size: 1387 647
ScaI AGT'ACT
Cuts at: 0 75 2034
Size: 75 1959
SexAI A'CCwGG_T
Cuts at: 0 635 2034
Size: 635 1399
SfcI C'TryA_G
Cuts at: 0 61 1025 1037 1164 1636 1739 2034
Size: 61 964 12 127 472 103 295
Fragments arranged by size:
964 472 295 127 103 61 12
SmlI C'TyrA_G
Cuts at: 0 1745 2034
Size: 1745 289
StuI AGG'CCT
Cuts at: 0 951 1832 2034
Size: 951 881 202
Fragments arranged by size:
951 881 202
StyI C'CwwG_G
Cuts at: 0 200 1577 1781 2034
Size: 200 1377 204 253
Fragments arranged by size:
1377 253 204 200
TatI w'GTAC_w
Cuts at: 0 73 1103 2034
Size: 73 1030 931
Fragments arranged by size:
1030 931 73
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 273 1075 2034
Size: 273 802 959
Fragments arranged by size:
959 802 273
XhoI C'TCGA_G
Cuts at: 0 1745 2034
Size: 1745 289
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AleI AlfI AlwNI
ApaI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII Bme1580I BmrI
BpmI Bpu10I BsaAI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BstEII
BstXI BstYI BtgI BtsI DraI EaeI EarI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I MfeI MmeI
MscI MslI MspA1I NcoI NsiI NspI PfoI PmlI
PpuMI PshAI PspOMI PspXI PstI PvuII SanDI ScaI
SexAI SfcI SmlI StuI StyI TatI XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BbeI BlpI BmgBI
BmtI BplI BpuEI BsaI BsaBI BsaHI BsiEI BsiWI
BsmBI BspEI BsrBI BsrFI BssHII BssSI BstAPI BstBI
BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI
EciI EcoICRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PflMI PmeI
PpiI PsiI PsrI PvuI RsrII SacI SacII SalI
SapI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TstI Tth111I
XbaI XmaI XmnI ZraI