################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BclI BcnI BfaI BfrI BglI BlnI BsaAI BsaBI BsaI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FbaI FseI FspI HaeII HincII HindII HindIII HinfI HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AcsI 595 GAATTC [GA]^AATT[CT] AcyI 520 GACGCC G[GA]^CG[CT]C AfaI 555 GTAC GT^AC AflIII 742 ACATGT A^C[GA][CT]GT AlwI 27 GGATC GGATC(4/5) AocI 57 CCTGAGG CC^T[ACGT]AGG ApoI 595 GAATTC [GA]^AATT[CT] AseI 205 ATTAAT AT^TAAT AsnI 205 ATTAAT AT^TAAT AvaII 177 GGACC G^G[AT]CC BciVI 318 GGATAC GTATCC(6/5) BglII 152 AGATCT A^GATCT BlpI 643 GCTGAGC GC^T[ACGT]AGC Bpu1102I 643 GCTGAGC GC^T[ACGT]AGC BsaHI 520 GACGCC G[GA]^CG[CT]C BsaWI 128 ACCGGA [AT]^CCGG[AT] BseRI 368 GAGGAG GAGGAG(10/8) BsmAI 565 GTCTC GTCTC(1/5) BsmFI 176 GGGAC GGGAC(10/14) BsmI 293 GCATTC GAATGC(1/-1) BspLU11I 742 ACATGT A^CATGT BspMI 109 ACCTGC ACCTGC(4/8) BsrDI 348 CATTGC GCAATG(2/0) BstF5I 678 GGATG GGATG(2/0) BstYI 152 AGATCT [GA]^GATC[CT] Bsu36I 57 CCTGAGG CC^T[ACGT]AGG CelII 643 GCTGAGC GC^T[ACGT]AGC DraII 176 GGGACCT [GA]G^G[ACGT]CC[CT] DsaI 140 CCGTGG C^C[GA][CT]GG EaeI 440 TGGCCG [CT]^GGCC[GA] EarI 481 CTCTTC CTCTTC(1/4) Eco81I 57 CCTGAGG CC^T[ACGT]AGG EcoNI 57 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 176 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 595 GAATTC G^AATTC EcoT14I 158 CCAAGG C^C[AT][AT]GG EcoT22I 345 ATGCAT ATGCA^T FauI 101 CCCGC CCCGC(4/6) FokI 678 GGATG GGATG(9/13) HapII 129 CCGG C^CGG Hin1I 520 GACGCC G[GA]^CG[CT]C HpaII 129 CCGG C^CGG Ksp632I 481 CTCTTC CTCTTC(1/4) MaeIII 106 GTCAC ^GT[ACGT]AC MflI 152 AGATCT [GA]^GATC[CT] MspA1I 323 CCGCTG C[AC]G^C[GT]G MspI 129 CCGG C^CGG NlaIII 743 CATG CATG^ NsiI 345 ATGCAT ATGCA^T NspI 742 ACATGT [GA]CATG^[CT] PflMI 428 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 176 GGGACCT [GA]G^G[AT]CC[CT] PshBI 205 ATTAAT AT^TAAT RsaI 555 GTAC GT^AC SacI 371 GAGCTC GAGCT^C ScaI 554 AGTACT AGT^ACT SfcI 363 CTATAG C^T[GA][CT]AG SmlI 374 CTCAAG C^T[CT][GA]AG StyI 158 CCAAGG C^C[AT][AT]GG Tsp45I 106 GTCAC ^GT[GC]AC Van91I 428 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 152 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 31 CGCG CG^CG 103 CGCG AlwNI 52 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG 433 CAGTGGCTG AspHI 124 GAGCAC G[AT]GC[AT]^C 371 GAGCTC BanI 12 GGCACC G^G[CT][GA]CC 136 GGCACC BanII 371 GAGCTC G[GA]GC[CT]^C 662 GGGCTC BbsI 192 GAAGAC GAAGAC(2/6) 391 GTCTTC BpmI 526 CTCCAG CTGGAG(16/14) 649 CTGGAG BpuAI 192 GAAGAC GAAGAC(2/6) 391 GTCTTC BsiHKAI 124 GAGCAC G[AT]GC[AT]^C 371 GAGCTC BstUI 31 CGCG CG^CG 103 CGCG BstXI 251 CCATTGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 632 CCAATGTGCTGG CfoI 113 GCGC GCG^C 505 GCGC Cfr13I 177 GGACC G^G[ACGT]CC 534 GGCCC DpnII 28 GATC ^GATC 153 GATC HhaI 113 GCGC GCG^C 505 GCGC HinP1I 113 GCGC G^CGC 505 GCGC HphI 580 GGTGA GGTGA(8/7) 107 TCACC MboI 28 GATC ^GATC 153 GATC MseI 149 TTAA T^TAA 206 TTAA MslI 165 CATTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 252 CATTGGAGTG MvnI 31 CGCG CG^CG 103 CGCG NdeII 28 GATC ^GATC 153 GATC Sau3AI 28 GATC ^GATC 153 GATC Sau96I 177 GGACC G^G[ACGT]CC 534 GGCCC TaqI 266 TCGA T^CGA 666 TCGA Tru9I 149 TTAA T^TAA 206 TTAA Tsp509I 238 AATT ^AATT 596 AATT TthHB8I 266 TCGA T^CGA 666 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AciI AluI BbvI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstNI Cac8I DdeI EcoRII Fnu4HI HaeIII HgaI ItaI MboII MnlI MvaI MwoI NlaIV ScrFI SfaNI TseI TspRI XcmI