Restriction map for
pFN21AE3607
(Circular) (Six-base) MAPSORT of: pFN21AE3607.seq Check: 4459 from: 1 to: 5458
pFN21AE3607 5458 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2123 2123
Size: 5458
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3268 278
Size: 53 83 186 936 1732 2468
Fragments arranged by size:
2468 1732 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2801 1937
Size: 864 4594
Acc65I G'GTAC_C
Cuts at: 2779 2779
Size: 5458
AclI AA'CG_TT
Cuts at: 1563 3585 3958 1563
Size: 2022 373 3063
Fragments arranged by size:
3063 2022 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2290 2477 3521 4905 5173 1632
Size: 658 187 1044 1384 268 1917
Fragments arranged by size:
1917 1384 1044 658 268 187
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5261
AflIII A'CryG_T
Cuts at: 1203 2747 4357 5334 1203
Size: 1544 1610 977 1327
Fragments arranged by size:
1610 1544 1327 977
AgeI A'CCGG_T
Cuts at: 4289 4289
Size: 5458
AhdI GACnn_n'nnGTC
Cuts at: 4187 4187
Size: 5458
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5458
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5458
AlwNI CAG_nnn'CTG
Cuts at: 2062 2443 3201 4773 2062
Size: 381 758 1572 2747
Fragments arranged by size:
2747 1572 758 381
ApaLI G'TGCA_C
Cuts at: 3515 4671 3515
Size: 1156 4302
ApoI r'AATT_y
Cuts at: 1493 2600 2767 2993 3145 5254 1493
Size: 1107 167 226 152 2109 1697
Fragments arranged by size:
2109 1697 1107 226 167 152
AseI AT'TA_AT
Cuts at: 160 1032 2211 4012 160
Size: 872 1179 1801 1606
Fragments arranged by size:
1801 1606 1179 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5458
AvaI C'yCGr_G
Cuts at: 1870 1943 2783 1870
Size: 73 840 4545
Fragments arranged by size:
4545 840 73
BamHI G'GATC_C
Cuts at: 2788 3155 2788
Size: 367 5091
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 2141 2779 4234 618
Size: 325 217 627 230 124 638 1455 1842
Fragments arranged by size:
1842 1455 638 627 325 230 217 124
BanII G_rGCy'C
Cuts at: 1391 2380 2671 2777 1391
Size: 989 291 106 4072
Fragments arranged by size:
4072 989 291 106
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2204 2389 928
Size: 971 305 185 3997
Fragments arranged by size:
3997 971 305 185
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3649 3683 1903
Size: 34 1712 34 3678
Fragments arranged by size:
3678 1712 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2317 3350 4560 2317
Size: 1033 1210 3215
Fragments arranged by size:
3215 1210 1033
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5458
BfrBI ATG'CAT
Cuts at: 2352 2352
Size: 5458
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4069 136
Size: 107 122 71 631 605 2397 1525
Fragments arranged by size:
2397 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2157 5453 2157
Size: 3296 2162
BlpI GC'TnA_GC
Cuts at: 2649 2875 2649
Size: 226 5232
Bme1580I G_kGCm'C
Cuts at: 1790 2091 3519 4675 1790
Size: 301 1428 1156 2573
Fragments arranged by size:
2573 1428 1156 301
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5458
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2050 2572 2750
Size: 416 53 83 399 24 633 522 178
Cuts at: 2750 3189 4147 442
Size: 439 958 1753
Fragments arranged by size:
1753 958 633 522 439 416 399 178
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2516 2675 4118 1239
Size: 1277 159 1443 2579
Fragments arranged by size:
2579 1443 1277 159
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2364 3583 4448 4746 2364
Size: 1219 865 298 3076
Fragments arranged by size:
3076 1219 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4121 882
Size: 617 2622 2219
Fragments arranged by size:
2622 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3249 493
Size: 711 2045 2702
Fragments arranged by size:
2702 2045 711
BsaBI GATnn'nnATC
Cuts at: 5452 5452
Size: 5458
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2526 3265 3647 4282
Size: 53 83 186 936 993 739 382 635
Cuts at: 4282 275
Size: 1451
Fragments arranged by size:
1451 993 936 739 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2133 3891 4289 4563 4710 5121 2133
Size: 1758 398 274 147 411 2470
Fragments arranged by size:
2470 1758 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5233
Fragments arranged by size:
5233 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2388 1186
Size: 1202 4256
BseYI C'CCAG_C
Cuts at: 2293 2330 4661 5157 2293
Size: 37 2331 496 2594
Fragments arranged by size:
2594 2331 496 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4885
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2908 3669 4697 1540
Size: 275 186 907 761 1028 2301
Fragments arranged by size:
2301 1028 907 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2133 2380 3519 3604 4675 2133
Size: 247 1139 85 1071 2916
Fragments arranged by size:
2916 1139 1071 247 85
BsmI GAATG_Cn'
Cuts at: 2298 2969 3062 2298
Size: 671 93 4694
Fragments arranged by size:
4694 671 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2133 2380 2671 2777 3519 3604
Size: 399 301 42 247 291 106 742 85
Cuts at: 3604 4675 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 301 291 247 106
85 42
BspEI T'CCGG_A
Cuts at: 5121 5121
Size: 5458
BspHI T'CATG_A
Cuts at: 3347 5077 3347
Size: 1730 3728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2123 2813 844
Size: 1279 690 3489
Fragments arranged by size:
3489 1279 690
BsrBI CCG'CTC
Cuts at: 1394 1823 3345 1394
Size: 429 1522 3507
Fragments arranged by size:
3507 1522 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2352 3953 4127 65
Size: 1183 1104 1601 174 1396
Fragments arranged by size:
1601 1396 1183 1104 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4102 4289 1700
Size: 110 2292 187 2869
Fragments arranged by size:
2869 2292 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5458
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5458
BssSI C'ACGA_G
Cuts at: 3518 4530 3518
Size: 1012 4446
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5458
BstBI TT'CG_AA
Cuts at: 4262 4262
Size: 5458
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5458
BstXI CCAn_nnnn'nTGG
Cuts at: 2263 2644 2263
Size: 381 5077
BstYI r'GATC_y
Cuts at: 1972 2157 2788 3155 3540 3557 5009 5095 5107
Size: 185 631 367 385 17 1452 86 12
Cuts at: 5107 5453 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 631 385 367 346 185 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2063 2063
Size: 5458
BtgI C'CryG_G
Cuts at: 513 1065 2145 513
Size: 552 1080 3826
Fragments arranged by size:
3826 1080 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4719
Fragments arranged by size:
4719 683 56
BtsI GCAGTG_nn'
Cuts at: 2444 2974 3768 3788 2444
Size: 530 794 20 4114
Fragments arranged by size:
4114 794 530 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5458
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5423
DraI TTT'AAA
Cuts at: 2762 2762
Size: 5458
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4465 809
Size: 507 3149 1802
Fragments arranged by size:
3149 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2445 2905 3794
Size: 54 1425 50 275 18 615 460 889
Cuts at: 3794 5423 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 615 460 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2905 1537
Size: 275 1093 4090
Fragments arranged by size:
4090 1093 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2492 3388 1353
Size: 1139 896 3423
Fragments arranged by size:
3423 1139 896
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2060 4045 4419 4565 387
Size: 1064 138 471 1985 374 146 1280
Fragments arranged by size:
1985 1280 1064 471 374 146 138
EcoICRI GAG'CTC
Cuts at: 2378 2378
Size: 5458
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2290 2477 2516 2675 3521 4118 4905
Size: 393 658 187 39 159 846 597 787
Cuts at: 4905 5173 1239
Size: 268 1524
Fragments arranged by size:
1524 846 787 658 597 393 268 187
159 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2066 2066
Size: 5458
EcoO109I rG'GnC_Cy
Cuts at: 2182 2182
Size: 5458
EcoRI G'AATT_C
Cuts at: 2600 2767 3145 2600
Size: 167 378 4913
Fragments arranged by size:
4913 378 167
FspI TGC'GCA
Cuts at: 3964 3964
Size: 5458
HaeII r_GCGC'y
Cuts at: 1678 4605 1678
Size: 2927 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2142 2174 2402 2434 4099
Size: 32 135 32 570 32 228 32 1665
Cuts at: 4099 4131 4173 4205 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1665 570 228 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2802 3048 669
Size: 988 281 864 246 3079
Fragments arranged by size:
3079 988 864 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5458
HpaI GTT'AAC
Cuts at: 3048 3048
Size: 5458
KpnI G_GTAC'C
Cuts at: 2783 2783
Size: 5458
MfeI C'AATT_G
Cuts at: 3057 3057
Size: 5458
MluI A'CGCG_T
Cuts at: 5334 5334
Size: 5458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4572 4756 1305
Size: 3267 184 2007
Fragments arranged by size:
3267 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3636
Fragments arranged by size:
3636 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2174 2261 3416 3775 3934 518
Size: 648 1008 87 1155 359 159 2042
Fragments arranged by size:
2042 1155 1008 648 359 159 87
MspA1I CmG'CkG
Cuts at: 1606 2330 2874 3551 4699 4944 1606
Size: 724 544 677 1148 245 2120
Fragments arranged by size:
2120 1148 724 677 544 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4906
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5458
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5458
NotI GC'GGCC_GC
Cuts at: 2905 2905
Size: 5458
NsiI A_TGCA'T
Cuts at: 2354 2354
Size: 5458
NspI r_CATG'y
Cuts at: 1132 1229 2751 2816 4361 1132
Size: 97 1522 65 1545 2229
Fragments arranged by size:
2229 1545 1522 97 65
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5458
PciI A'CATG_T
Cuts at: 2747 4357 2747
Size: 1610 3848
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2439 1108
Size: 1331 4127
PmeI GTTT'AAAC
Cuts at: 2762 2762
Size: 5458
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3527 3559 5040 5072 3527
Size: 32 1481 32 3913
Fragments arranged by size:
3913 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2182 2182
Size: 5458
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5458
PsiI TTA'TAA
Cuts at: 3028 3028
Size: 5458
PstI C_TGCA'G
Cuts at: 830 2810 830
Size: 1980 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5458
SacI G_AGCT'C
Cuts at: 2380 2380
Size: 5458
SalI G'TCGA_C
Cuts at: 1936 2800 1936
Size: 864 4594
SbfI CC_TGCA'GG
Cuts at: 2810 2810
Size: 5458
ScaI AGT'ACT
Cuts at: 2561 3706 5418 2561
Size: 1145 1712 2601
Fragments arranged by size:
2601 1712 1145
SfcI C'TryA_G
Cuts at: 826 1045 2368 2806 3941 4622 4813 826
Size: 219 1323 438 1135 681 191 1471
Fragments arranged by size:
1471 1323 1135 681 438 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5458
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5458
SmaI CCC'GGG
Cuts at: 1872 2785 1872
Size: 913 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2379 3562 4463 4725 820
Size: 197 926 436 1183 901 262 1553
Fragments arranged by size:
1553 1183 926 901 436 262 197
SnaBI TAC'GTA
Cuts at: 493 3249 493
Size: 2756 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5458
SphI G_CATG'C
Cuts at: 1132 2816 1132
Size: 1684 3774
SspI AAT'ATT
Cuts at: 5 52 3382 5
Size: 47 3330 2081
Fragments arranged by size:
3330 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5458
StyI C'CwwG_G
Cuts at: 513 1065 2163 2897 513
Size: 552 1098 734 3074
Fragments arranged by size:
3074 1098 734 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3485 3502 3655 3840 4285 325
Size: 1255 1905 17 153 185 445 1498
Fragments arranged by size:
1905 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2559 3704 5416
Size: 274 80 33 51 1443 582 1145 1712
Cuts at: 5416 96
Size: 138
Fragments arranged by size:
1712 1443 1145 582 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5418 5450 5418
Size: 32 5426
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5458
XbaI T'CTAG_A
Cuts at: 2794 2794
Size: 5458
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2170 2260 2440 2011
Size: 159 90 180 5029
Fragments arranged by size:
5029 180 159 90
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5458
XmaI C'CCGG_G
Cuts at: 1870 2783 1870
Size: 913 4545
XmnI GAAnn'nnTTC
Cuts at: 3587 3587
Size: 5458
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3266 276
Size: 53 83 186 936 1732 2468
Fragments arranged by size:
2468 1732 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI